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Expert Review of Molecular Diagnostics Volume 15, 2015 - Issue 12
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Towards the realization of clinical extracellular vesicle diagnostics: challenges and opportunities

Akira YokoiDivision of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan
,
Yusuke YoshiokaDivision of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, Japan
&
Takahiro OchiyaDivision of Molecular and Cellular Medicine, National Cancer Center Research Institute, Tokyo, JapanCorrespondence[email protected]
Pages 1555-1566 | Published online: 26 Oct 2015

References

  • Papers of special note have been highlighted as:
  • * of interest
  • ** of considerable interest
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* One of the novel methodologies which overcome several obstacles in EV research. The ExoScreen assay, can exploit circulating EVs in the serum via an amplified luminescent proximity homogeneous assay using photosensitizer-beads and 2 types of antibodies without the need for any purification steps

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* One of the novel methodologies which overcome several obstacles in EV research. The nPLEX assay, which is based on surface plasmon resonance that enables the label-free, high-throughput analysis of proteins in EVs

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** First study to identify miRNAs in EVs released from human and murine mast cell lines. This is one of the pioneering studies laid the foundation for EV-associated RNA research

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* This study was one of the first to describe that the miRNA in EVs can serve as a disease biomarker. This also described the relevance of the correlation between EpCAM and EV

** First study to identify the function of EV. The value of EVs began to be recognized, and EVs were no longer considered inconsequential

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