Advanced search
Publication Cover
Expert Review of Molecular Diagnostics Volume 12, 2012 - Issue 7
Submit an article Journal homepage
155
Views
20
CrossRef citations to date
0
Altmetric
Theme: Infectious Diseases - Review

PCR for the molecular diagnosis of mycotic keratitis

Philip A Thomas Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirapalli 620001, India; Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirapalli 620001, India. [email protected]
,
Philip A Teresa Institute of Ophthalmology, Joseph Eye Hospital, Tiruchirapalli 620001, India
,
Joseph Theodore Thomas Diagnostic and Research Centre, Tiruchirapalli 620001, India
&
Pitchairaj Geraldine Department of Animal Science, Bharathidasan University, Tiruchirapalli 620024, India; Department of Animal Science, Bharathidasan University, Tiruchirapalli 620024, India
Pages 703-718 | Published online: 09 Jan 2014

References

  • Tuft SJ, Shortt AJ. Surgical rehabilitation following severe ocular burns. Eye (Lond.) 23(10), 1966–1971 (2009).
  • Wang L, Sun S, Jing Y, Han L, Zhang H, Yue J. Spectrum of fungal keratitis in central China. Clin. Experiment. Ophthalmol. 37(8), 763–771 (2009).
  • Nath R, Baruah S, Saikia L, Devi B, Borthakur AK, Mahanta J. Mycotic corneal ulcers in upper Assam. Indian J. Ophthalmol. 59(5), 367–371 (2011).
  • Keay LJ, Gower EW, Iovieno A et al. Clinical and microbiological characteristics of fungal keratitis in the United States, 2001–2007: a multicenter study. Ophthalmology 118(5), 920–926 (2011).
  • Thomas PA, Geraldine P. Infectious keratitis. Curr. Opin. Infect. Dis. 20(2), 129–141 (2007).
  • Thomas PA, Leck AK, Myatt M. Characteristic clinical features as an aid to the diagnosis of suppurative keratitis caused by filamentous fungi. Br. J. Ophthalmol. 89(12), 1554–1558 (2005).
  • Vaddavalli PK, Garg P, Sharma S, Sangwan VS, Rao GN, Thomas R. Role of confocal microscopy in the diagnosis of fungal and Acanthamoeba keratitis. Ophthalmology 118(1), 29–35 (2011).
  • Alfonso EC. Genotypic identification of Fusarium species from ocular sources: comparison to morphologic classification and antifungal sensitivity testing (an AOS thesis). Trans. Am. Ophthalmol. Soc. 106, 227–239 (2008).
  • Thomas PA. Current perspectives on ophthalmic mycoses. Clin. Microbiol. Rev. 16(4), 730–797 (2003).
  • Thomas PA, Kaliamurthy J, Jesudasan CA, Geraldine P. Use of chlorazol black E mounts of corneal scrapes for diagnosis of filamentous fungal keratitis. Am. J. Ophthalmol. 145(6), 971–976 (2008).
  • Marangon FB, Miller D, Alfonso EC. Impact of prior therapy on the recovery and frequency of corneal pathogens. Cornea 23(2), 158–164 (2004).
  • Keay L, Edwards K, Naduvilath T et al. Microbial keratitis predisposing factors and morbidity. Ophthalmology 113(1), 109–116 (2006).
  • Leck AK, Thomas PA, Hagan M et al. Aetiology of suppurative corneal ulcers in Ghana and south India, and epidemiology of fungal keratitis. Br. J. Ophthalmol. 86(11), 1211–1215 (2002).
  • Seitzman GD, Cevallos V, Margolis TP. Rose bengal and lissamine green inhibit detection of herpes simplex virus by PCR. Am. J. Ophthalmol. 141(4), 756–758 (2006).
  • Procop GW. Molecular diagnostics for the detection and characterization of microbial pathogens. Clin. Infect. Dis. 45(Suppl. 2), S99–S111 (2007).
  • Perlin DS. Antifungal drug resistance: do molecular methods provide a way forward? Curr. Opin. Infect. Dis. 22(6), 568–573 (2009).
  • Alexandrakis G, Sears M, Gloor P. Postmortem diagnosis of Fusarium panophthalmitis by the polymerase chain reaction. Am. J. Ophthalmol. 121(2), 221–223 (1996).
  • Alexandrakis G, Jalali S, Gloor P. Diagnosis of Fusarium keratitis in an animal model using the polymerase chain reaction. Br. J. Ophthalmol. 82(3), 306–311 (1998).
  • He D, Hao J, Zhang B et al. Pathogenic spectrum of fungal keratitis and specific identification of Fusarium solani. Invest. Ophthalmol. Vis. Sci. 52(5), 2804–2808 (2011).
  • Iwen PC, Hinrichs SH, Rupp ME. Utilization of the internal transcribed spacer regions as molecular targets to detect and identify human fungal pathogens. Med. Mycol. 40(1), 87–109 (2002).
  • Perlin DS, Zhao Y. Molecular diagnostic platforms for detecting Aspergillus. Med. Mycol. 47(Suppl. 1), S223–S232 (2009).
  • Itahashi M, Higaki S, Fukuda M, Shimomura Y. Detection and quantification of pathogenic bacteria and fungi using real-time polymerase chain reaction by cycling probe in patients with corneal ulcer. Arch. Ophthalmol. 128(5), 535–540 (2010).
  • Ferrer C, Alió JL. Evaluation of molecular diagnosis in fungal keratitis. Ten years of experience. J. Ophthalmic Inflamm. Infect. 1(1), 15–22 (2011).
  • Goldschmidt P, Degorge S, Benallaoua D et al. New strategy for rapid diagnosis and characterization of keratomycosis. Ophthalmology 119(5), 945–950 (2012).
  • Kumar M, Shukla PK. Use of PCR targeting of internal transcribed spacer regions and single-stranded conformation polymorphism analysis of sequence variation in different regions of rRNA genes in fungi for rapid diagnosis of mycotic keratitis. J. Clin. Microbiol. 43(2), 662–668 (2005).
  • Kumar M, Mishra NK, Shukla PK. Sensitive and rapid polymerase chain reaction-based diagnosis of mycotic keratitis through single-stranded conformation polymorphism. Am. J. Ophthalmol. 140(5), 851–857 (2005).
  • Gaudio PA, Gopinathan U, Sangwan V, Hughes TE. Polymerase chain reaction-based detection of fungi in infected corneas. Br. J. Ophthalmol. 86(7), 755–760 (2002).
  • Vengayil S, Panda A, Satpathy G et al. Polymerase chain reaction-guided diagnosis of mycotic keratitis: a prospective evaluation of its efficacy and limitations. Invest. Ophthalmol. Vis. Sci. 50(1), 152–156 (2009).
  • Ferrer C, Colom F, Frasés S, Mulet E, Abad JL, Alió JL. Detection and identification of fungal pathogens by PCR and by ITS2 and 5.8S ribosomal DNA typing in ocular infections. J. Clin. Microbiol. 39(8), 2873–2879 (2001).
  • Ferrer C, Muñoz G, Alió JL, Abad JL, Colomm F. Polymerase chain reaction diagnosis in fungal keratitis caused by Alternaria alternata. Am. J. Ophthalmol. 133(3), 398–399 (2002).
  • Mancini N, Ossi CM, Perotti M et al. Direct sequencing of Scedosporium apiospermum DNA in the diagnosis of a case of keratitis. J. Med. Microbiol. 54(Pt 9), 897–900 (2005).
  • Mancini N, Perotti M, Ossi CM et al. Rapid molecular identification of fungal pathogens in corneal samples from suspected keratomycosis cases. J. Med. Microbiol. 55(Pt 11), 1505–1509 (2006).
  • Bagyalakshmi R, Therese KL, Madhavan HN. Application of semi-nested polymerase chain reaction targeting internal transcribed spacer region for rapid detection of panfungal genome directly from ocular specimens. Indian J. Ophthalmol. 55(4), 261–265 (2007).
  • Bagyalakshmi R, Therese KL, Prasanna S, Madhavan HN. Newer emerging pathogens of ocular nonsporulating molds (NSM) identified by polymerase chain reaction (PCR)-based DNA sequencing technique targeting internal transcribed spacer (ITS) region. Curr. Eye Res. 33(2), 139–147 (2008).
  • Embong Z, Wan Hitam WH, Yean CY et al. Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis. BMC Ophthalmol. 8, 7 (2008).
  • Ghosh A, Basu S, Datta H, Chattopadhyay D. Evaluation of polymerase chain reaction-based ribosomal DNA sequencing technique for the diagnosis of mycotic keratitis. Am. J. Ophthalmol. 144(3), 396–403 (2007).
  • Kim E, Chidambaram JD, Srinivasan M et al. Prospective comparison of microbial culture and polymerase chain reaction in the diagnosis of corneal ulcer. Am. J. Ophthalmol. 146(5), 714–723, 723.e1 (2008).
  • Menassa N, Bosshard PP, Kaufmann C, Grimm C, Auffarth GU, Thiel MA. Rapid detection of fungal keratitis with DNA-stabilizing FTA filter paper. Invest. Ophthalmol. Vis. Sci. 51(4), 1905–1910 (2010).
  • Badiee P, Alborzi A, Nejabat M. Detection of Aspergillus keratitis in ocular infections by culture and molecular method. Int. Ophthalmol. 31(4), 291–296 (2011).
  • Balne PK, Reddy AK, Kodiganti M, Gorli SR, Garg P. Evaluation of three PCR assays for the detection of fungi in patients with mycotic keratitis. Br. J. Ophthalmol. 96(6), 911–912 (2012).
  • Lau A, Chen S, Sorrell T et al. Development and clinical application of a panfungal PCR assay to detect and identify fungal DNA in tissue specimens. J. Clin. Microbiol. 45(2), 380–385 (2007).
  • Rishi K, Font RL. Keratitis caused by an unusual fungus, Phoma species. Cornea 22(2), 166–168 (2003).
  • Ferrer C, Montero J, Alió JL, Abad JL, Ruiz-Moreno JM, Colom F. Rapid molecular diagnosis of posttraumatic keratitis and endophthalmitis caused by Alternaria infectoria. J. Clin. Microbiol. 41(7), 3358–3360 (2003).
  • Ferrer C, Alió JL, Mulet ME, Rodríguez AE, Colom MF. Polymerase chain reaction and DNA typing for diagnosis of infectious crystalline keratopathy. J. Cataract Refract. Surg. 32(12), 2142–2145 (2006).
  • Donnio A, Desbois N, Boiron P et al. [Mycotic keratitis and endophthalmitis caused by unusual fungi: Lasiodiplodia theobromae]. J. Fr. Ophtalmol. 29(2), e4 (2006).
  • Suzuki T, Hori N, Miyake T, Hori Y, Mochizuki K. Keratitis caused by a rare fungus, Malassezia restricta. Jpn. J. Ophthalmol. 51(4), 292–294 (2007).
  • Yoon S, Kim S, Lee KA, Kim H. [A case of Scedosporium apiospermum keratitis confirmed by a molecular genetic method]. Korean J. Lab. Med. 28(4), 307–311 (2008).
  • Laich FS, Alcoba-Flórez J, Pérez-Roth E, Bahaya Y, Delgado JL, Méndez-Alvarez S. Molecular characterization of Alternaria alternata causing ocular infection: detection of IGS-RFLP intraspecific polymorphism. Med. Mycol. 46(6), 615–619 (2008).
  • Ferrer C, Pérez-Santonja JJ, Rodríguez AE et al. New pyrenochaeta species causing keratitis. J. Clin. Microbiol. 47(5), 1596–1598 (2009).
  • Guarro J, Rubio C, Gené J et al. Case of keratitis caused by an uncommon Fusarium species. J. Clin. Microbiol. 41(12), 5823–5826 (2003).
  • Kredics L, Varga J, Kocsubé S et al. Case of keratitis caused by Aspergillus tamarii. J. Clin. Microbiol. 45(10), 3464–3467 (2007).
  • Oechsler RA, Feilmeier MR, Ledee DR et al. Utility of molecular sequence analysis of the ITS rRNA region for identification of Fusarium spp. from ocular sources. Invest. Ophthalmol. Vis. Sci. 50(5), 2230–2236 (2009).
  • Manikandan P, Vismer HF, Kredics L et al. Corneal ulcer due to Neocosmospora vasinfecta in an immunocompetent patient. Med. Mycol. 46(3), 279–284 (2008).
  • Kredics L, Varga J, Kocsubé S et al. Infectious keratitis caused by Aspergillus tubingensis. Cornea 28(8), 951–954 (2009).
  • Azor M, Gené J, Cano J, Manikandan P, Venkatapathy N, Guarro J. Less-frequent Fusarium species of clinical interest: correlation between morphological and molecular identification and antifungal susceptibility. J. Clin. Microbiol. 47(5), 1463–1468 (2009).
  • Manikandan P, Varga J, Kocsubé S et al. Mycotic keratitis due to Aspergillus nomius. J. Clin. Microbiol. 47(10), 3382–3385 (2009).
  • Dong YL, Xie LX, Yin HM, Sun SY, Huang HN. [Preliminary study on the use of DNA suspension array for the identification of corneal pathogenic fungi]. Zhonghua. Yan Ke Za Zhi. 45(2), 110–114 (2009).
  • Manikandan P, Varga J, Kocsubé S et al. Keratitis caused by the recently described new species Aspergillus brasiliensis: two case reports. J. Med. Case Rep. 4, 68 (2010).
  • Shivaprakash MR, Appannanavar SB, Dhaliwal M et al. Colletotrichum truncatum: an unusual pathogen causing mycotic keratitis and endophthalmitis. J. Clin. Microbiol. 49(8), 2894–2898 (2011).

Website

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Order Reprints Request Corporate Permissions

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

Request Academic Permissions

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.