Nano-microparticles as immune adjuvants: correlating particle sizes and the resultant immune responses

References

• Davis JP. Experience with hepatitis A and B vaccines. Am. J. Med.118(10 Suppl. 1), 7–15 (2005).
   PubMed Web of Science ®Google Scholar
• Look M, Bandyopadhyay A, Blum JS, Fahmy TM. Application of nanotechnologies for improved immune response against infectious diseases in the developing world. Adv. Drug Deliv. Rev.62(4–5), 378–393 (2009).
   PubMed Web of Science ®Google Scholar
• O’Hagan DT, Valiante NM. Recent advances in the discovery and delivery of vaccine adjuvants. Nat. Rev. Drug Discov.2(9), 727–735 (2003).
   PubMed Web of Science ®Google Scholar
• Perrie Y, Mohammed AR, Kirby DJ, McNeil SE, Bramwell VW. Vaccine adjuvant systems: enhancing the efficacy of sub-unit protein antigens. Int. J. Pharm.364(2), 272–280 (2008).
   PubMed Web of Science ®Google Scholar
• Copland MJ, Baird MA, Rades T et al. Liposomal delivery of antigen to human dendritic cells. Vaccine21(9–10), 883–890 (2003).
   PubMed Web of Science ®Google Scholar
• Schijns V. Immunological concepts for vaccine adjuvant activity. Curr. Opin. Immunol.12(4), 456–463 (2000).
   PubMed Web of Science ®Google Scholar
• Caputo A, Sparnacci K, Ensoli B, Tondelli L. Functional polymeric nano/microparticles for surface adsorption and delivery of protein and DNA vaccines. Curr. Drug Deliv.5(4), 230–242 (2008).
   PubMedGoogle Scholar
• Petrovsky N, Aguilar JC. Vaccine adjuvants: current state and future trends. Immunol. Cell Biol.82(5), 488–496 (2004).
   PubMed Web of Science ®Google Scholar
• Chang S, Warner J, Liang L, Fairman J. A novel vaccine adjuvant for recombinant flu antigens. Biologicals37(3), 141–147 (2009).
   PubMed Web of Science ®Google Scholar
• Singh M, Briones M, Ott G, O’Hagan DT. Cationic microparticles: a potent delivety system for DNA vaccines. Proc. Natl Acad. Sci. USA97, 811–816 (2000).
   PubMed Web of Science ®Google Scholar
• Kalkanidis M, Pietersz GA, Xiang SD et al. Methods for nano-particle based vaccine formulation and evaluation of their immunogenicity. Methods40(1), 20–29 (2006).
   PubMed Web of Science ®Google Scholar
• Peek LJ, Middaugh CR, Berkland C. Nanotechnology in vaccine delivery. Adv. Drug Deliv. Rev.60(8), 915–928 (2008).
   PubMed Web of Science ®Google Scholar
• Scheerlinck J-PY, Greenwood DLV. Virus-sized vaccine delivery systems. Drug Discov. Today13(19–20), 882–887 (2008).
   PubMed Web of Science ®Google Scholar
• Aguilar JC, Rodríguez EG. Vaccine adjuvants revisited. Vaccine25(19), 3752–3762 (2007).
   PubMed Web of Science ®Google Scholar
• O’Hagan DT, Singh M. Microparticles as vaccine adjuvants and delivery systems. Expert Rev. Vaccines2, 269–283 (2003).
   PubMedGoogle Scholar
• Schmidt CS, Morrow WJW, Sheikh NA. Smart adjuvants. Expert Rev. Vaccines6, 391–400 (2007).
   PubMed Web of Science ®Google Scholar
• SayIn B, Somavarapu S, Li XW, Sesardic D, Senel S, Alpar OH. TMC-MCC (N-trimethyl chitosan-mono-N-carboxymethyl chitosan) nanocomplexes for mucosal delivery of vaccines. Eur. J. Pharm. Sci.38(4), 362–369 (2009).
   PubMed Web of Science ®Google Scholar
• Gómez S, Gamazo C, San Roman B et al. A novel nanoparticulate adjuvant for immunotherapy with Lolium perenne. J. Immunol. Methods348(1–2), 1–8 (2009).
   PubMed Web of Science ®Google Scholar
• Gunaseelan S, Gunaseelan K, Deshmukh M, Zhang X, Sinko PJ. Surface modifications of nanocarriers for effective intracellular delivery of anti-HIV drugs. Adv. Drug Deliv. Rev.62(4–5), 518–531 (2010).
   PubMed Web of Science ®Google Scholar
• Reed SG, Bertholet S, Coler RN, Friede M. New horizons in adjuvants for vaccine development. Trends Immunol.30(1), 23–32 (2009).
   PubMed Web of Science ®Google Scholar
• Jones KS. Biomaterials as vaccine adjuvants. Biotechnol. Progress24(4), 807–814 (2008).
   PubMed Web of Science ®Google Scholar
• Mallapragada SK, Narasimhan B. Immunomodulatory biomaterials. Int. J. Pharm.364(2), 265–271 (2008).
   PubMed Web of Science ®Google Scholar
• Thomas C, Gupta V, Ahsan F. Influence of surface charge of PLGA particles of recombinant hepatitis B surface antigen in enhancing systemic and mucosal immune responses. Int. J. Pharm.379(1), 41–50 (2009).
   PubMed Web of Science ®Google Scholar
• Reddy ST, Rehor A, Schmoekel HG, Hubbell JA, Swartz MA. In vivo targeting of dendritic cells in lymph nodes with poly(propylene sulfide) nanoparticles. J. Controlled Release112(1), 26–34 (2006).
   PubMed Web of Science ®Google Scholar
• Walter E, Dreher D, Kok M et al. Hydrophilic poly(D,L-lactide-co-glycolide) microspheres for the delivery of DNA to human-derived macrophages and dendritic cells. J. Controlled Release76(1–2), 149–168 (2001).
   PubMed Web of Science ®Google Scholar
• Rice-Ficht AC, Arenas-Gamboa AM, Kahl-McDonagh MM, Ficht TA. Polymeric particles in vaccine delivery. Curr. Opin. Microbiol.13(1), 106–112 (2009).
   Web of Science ®Google Scholar
• Thomasin C, Corradin G, Men Y, Merkle HP, Gander B. Tetanus toxoid and synthetic malaria antigen containing poly(lactide)/poly(lactide-co-glycolide) microspheres: importance of polymer degradation and antigen release for immune response. J. Controlled Release41(1–2), 131–145 (1996).
   Web of Science ®Google Scholar
• Slütter B, Soema PC, Ding Z, Verheul R, Hennink W, Jiskoot W. Conjugation of ovalbumin to trimethyl chitosan improves immunogenicity of the antigen. J. Controlled Release143(2), 207–214 (2010).
   PubMed Web of Science ®Google Scholar
• O’Hagan DT. Microparticles and polymers for the mucosal delivery of vaccines. Adv. Drug Deliv. Rev.34(2–3), 305–320 (1998).
   PubMed Web of Science ®Google Scholar
• Kazzaz J, Neidleman J, Singh M, Ott G, O’Hagan DT. Novel anionic microparticles are a potent adjuvant for the induction of cytotoxic T lymphocytes against recombinant p55 gag from HIV-1. J. Controlled Release67(2–3), 347–356 (2000).
   PubMed Web of Science ®Google Scholar
• Jain S, Yap WT, Irvine DJ. Synthesis of protein-loaded hydrogel particles in an aqueous two-phase system for coincident antigen and CpG oligonucleotide delivery to antigen-presenting cells. Biomacromolecules6(5), 2590–2600 (2005).
   PubMed Web of Science ®Google Scholar
• Shen Z, Reznikoff G, Dranoff G, Rock K. Cloned dendritic cells can present exogenous antigens on both MHC class I and class II molecules. J. Immunol.158(6), 2723–2730 (1997).
   PubMed Web of Science ®Google Scholar
• Sharma S, Mukkur TKS, Benson HAE, Chen Y. Pharmaceutical aspects of intranasal delivery of vaccines using particulate systems. J. Pharm. Sci.98(3), 812–843 (2009).
   PubMed Web of Science ®Google Scholar
• Katare YK, Muthukumaran T, Panda AK. Influence of particle size, antigen load, dose and additional adjuvant on the immune response from antigen loaded PLA microparticles. Int. J. Pharm.301(1–2), 149–160 (2005).
   PubMed Web of Science ®Google Scholar
• Wendorf J, Singh M, Chesko J et al. A practical approach to the use of nanoparticles for vaccine delivery. J. Pharm. Sci.95(12), 2738–2750 (2006).
   PubMed Web of Science ®Google Scholar
• Gutierro I, Hernández RM, Igartua M, Gascón AR, Pedraz JL. Size dependent immune response after subcutaneous, oral and intranasal administration of BSA loaded nanospheres. Vaccine21(1–2), 67–77 (2002).
   PubMed Web of Science ®Google Scholar
• Jung T, Kamm W, Breitenbach A, Hungerer K-D, Hundt E, Kissel T. Tetanus toxoid loaded nanoparticles from sulfobutylated poly(vinyl alcohol)-graft-poly(lactide-co-glycolide): evaluation of antibody response after oral and nasal application in mice. Pharm. Res.18(3), 352–360 (2001).
   PubMed Web of Science ®Google Scholar
• Vila A, Sanchez A, Evora C, Soriano I, Jato J, Alonso M. PEG-PLA nanoparticles as carriers for nasal vaccine delivery. J. Aerosol Med.17, 174–185 (2004).
   PubMedGoogle Scholar
• Wendorf J, Chesko J, Kazzaz J et al. A comparison of anionic nanoparticles and microparticles as vaccine delivery systems. Hum. Vaccin.4(1), 44–49 (2008).
   PubMed Web of Science ®Google Scholar
• Manocha M, Pal PC, Chitralekha KT et al. Enhanced mucosal and systemic immune response with intranasal immunization of mice with HIV peptides entrapped in PLG microparticles in combination with Ulex Europaeus-I lectin as M cell target. Vaccine23(48–49), 5599–5617 (2005).
   PubMed Web of Science ®Google Scholar
• Ying M, Gander B, Merkle HP, Corradin G. Induction of sustained and elevated immune responses to weakly immunogenic synthetic malarial peptides by encapsulation in biodegradable polymer microspheres. Vaccine14(15), 1442–1450 (1996).
   PubMed Web of Science ®Google Scholar
• Panyam J, Labhasetwar V. Biodegradable nanoparticles for drug and gene delivery to cells and tissue. Adv. Drug Deliv. Rev.55(3), 329–347 (2003).
   PubMed Web of Science ®Google Scholar
• Elamanchili P, Diwan M, Cao M, Samuel J. Characterization of poly(D,L,-lactic-co-glycolic acid) based nanoparticulate system for enhanced delivery of antigens to dendritic cells. Vaccine22(19), 2406–2412 (2004).
   PubMed Web of Science ®Google Scholar
• Cui Z, Mumper RJ. Topical immunization using nanoengineered genetic vaccines. J. Controlled Release81(1–2), 173–184 (2002).
   PubMed Web of Science ®Google Scholar
• Cui Z, Mumper RJ. Genetic Immunization Using nanoparticles engineered from microemulsion precursors. Pharm. Res.19(7), 939–946 (2002).
   PubMed Web of Science ®Google Scholar
• Sloat BR, Sandoval MA, Hau AM, He Y, Cui Z. Strong antibody responses induced by protein antigens conjugated onto the surface of lecithin-based nanoparticles. J. Controlled Release141(1), 93–100 (2010).
   PubMed Web of Science ®Google Scholar
• Tobío M, Gref R, Sánchez A, Langer R, Alonso MJ. Stealth PLA-PEG nanoparticles as protein carriers for nasal administration. Pharm. Res.15(2), 270–275 (1998).
   PubMed Web of Science ®Google Scholar
• Rieger J, Freichels H, Imberty A et al. Polyester nanoparticles presenting mannose residues: toward the development of new vaccine delivery systems combining biodegradability and targeting properties. Biomacromolecules10(3), 651–657 (2009).
   PubMed Web of Science ®Google Scholar
• Andrianov AK, Marin A, Roberts BE. Polyphosphazene polyelectrolytes: a link between the formation of noncovalent complexes with antigenic proteins and immunostimulating activity. Biomacromolecules6(3), 1375–1379 (2005).
   PubMed Web of Science ®Google Scholar
• Zwiorek K, Bourquin C, Battiany J et al. Delivery by cationic gelatin nanoparticles strongly increases the immunostimulatory effects of CpG oligonucleotides. Pharm. Res.25(3), 551–562 (2008).
   PubMed Web of Science ®Google Scholar
• Coppi G, Iannuccelli V, Sala N, Bondi M. Alginate microparticles for polymyxin B Peyer’s patches uptake: microparticles for antibiotic oral administration. J. Microencap. Micro Nano Carriers21(8), 829–839 (2004).
   Google Scholar
• Okada H, Toguchi H. Biodegradable microspheres in drug delivery. Crit. Rev. Ther. Drug Carrier Syst.12(1), 1–99 (1995).
   PubMed Web of Science ®Google Scholar
• Florindo HF, Pandit S, Lacerda L, Gonçalves LMD, Alpar HO, Almeida AJ. The enhancement of the immune response against S. equi antigens through the intranasal administration of poly-[var e]-caprolactone-based nanoparticles. Biomaterials30(5), 879–891 (2009).
   PubMed Web of Science ®Google Scholar
• Freitas S, Merkle HP, Gander B. Microencapsulation by solvent extraction/evaporation: reviewing the state of the art of microsphere preparation process technology. J. Controlled Release102(2), 313–332 (2005).
   PubMed Web of Science ®Google Scholar
• Fifis T, Gamvrellis A, Crimeen-Irwin B et al. Size-dependent immunogenicity: therapeutic and protective properties of nano-vaccines against tumors. J. Immunol.173(5), 3148–3154 (2004).
   PubMed Web of Science ®Google Scholar
• Sharp F, Ruane D, Claas B et al. Uptake of particulate vaccine adjuvants by dendritic cells activates the NALP3 inflammasome. Proc. Natl Acad. Sci. USA106, 870–875 (2009).
   PubMed Web of Science ®Google Scholar
• Kreuter J. Nanoparticles and microparticles for drug and vaccine delivery. J. Anat.189, 503–505 (1996).
   PubMed Web of Science ®Google Scholar
• Mumper RJ, Cui Z, Oyewumi MO. Nanotemplate engineering of cell specific nanoparticles. J. Dispers. Sci. Technol.24(3), 569–588 (2003).
   Web of Science ®Google Scholar
• Monfardini C, Veronese M. Stabilization of substances in circulation. Bioconjug. Chem.9, 418–450 (1998).
   PubMed Web of Science ®Google Scholar
• Kim GJ, Nie S. Targeted cancer nanotherapy. Mater. Today8(8 Suppl. 1), 28–33 (2005).
   Web of Science ®Google Scholar
• Wu NZ, Da D, Rudoll TL, Needham D, Whorton AR, Dewhirst MW. Increased microvascular permeability contributes to preferential accumulation of stealth liposomes in tumor tissue. Cancer Res.53(16), 3765–3770 (1993).
   PubMed Web of Science ®Google Scholar
• Xiang SD, Scholzen A, Minigo G et al. Pathogen recognition and development of particulate vaccines: does size matter? Methods40(1), 1–9 (2006).
   PubMed Web of Science ®Google Scholar
• Kanchan V, Panda AK. Interactions of antigen-loaded polylactide particles with macrophages and their correlation with the immune response. Biomaterials28(35), 5344–5357 (2007).
   PubMed Web of Science ®Google Scholar
• Johansen P, Estevez F, Zurbriggen R et al. Towards clinical testing of a single-administration tetanus vaccine based on PLA/PLGA microspheres. Vaccine19(9–10), 1047–1054 (2000).
   PubMed Web of Science ®Google Scholar
• Johansen P, Gander B, Merkle HP, Sesardic D. Ambiguities in the preclinical quality assessment of microparticulate vaccines. Trends Biotechnol.18(5), 203–211 (2000).
   PubMed Web of Science ®Google Scholar
• Caputo A, Brocca-Cofano E, Castaldello A et al. Characterization of immune responses elicited in mice by intranasal co-immunization with HIV-1 Tat, gp140 DV2Env and/or SIV Gag proteins and the nontoxicogenic heat-labile Escherichia coli enterotoxin. Vaccine26(9), 1214–1227 (2008).
   PubMed Web of Science ®Google Scholar
• Mann JFS, Shakir E, Carter KC, Mullen AB, Alexander J, Ferro VA. Lipid vesicle size of an oral influenza vaccine delivery vehicle influences the Th1/Th2 bias in the immune response and protection against infection. Vaccine27(27), 3643–3649 (2009).
   PubMed Web of Science ®Google Scholar
• Cruz LJ, Tacken PJ, Fokkink R et al. Targeted PLGA nano- but not microparticles specifically deliver antigen to human dendritic cells via DC-SIGN in vitro. J. Controlled Release (2010) (In Press).
   PubMedGoogle Scholar
• Newman KD, Elamanchili P, Kwon GS, Samuel J. Uptake of poly(D,L-lactic-co-glycolic acid) microspheres by antigen-presenting cells in vivoJ. Biomed. Mater. Res.60(3), 480–486 (2002).
   PubMed Web of Science ®Google Scholar
• Tabata Y, Inoue Y, Ikada Y. Size effect on systemic and mucosal immune responses induced by oral administration of biodegradable microspheres. Vaccine14(17–18), 1677–1685 (1996).
   PubMed Web of Science ®Google Scholar
• Panyam J, Labhasetwar V. Dynamics of endocytosis and exocytosis ofpoly(D,L-lactide-co-glycolide) nanoparticles in vascular smooth muscle cells. Pharm. Res.20(2), 212–220 (2003).
   PubMed Web of Science ®Google Scholar
• Horisawa E, Kubota K, Tuboi I et al. Size-dependency of D,L-lactide/glycolide copolymer particulates for intra-articular delivery system on phagocytosis in rat synovium. Pharm. Res.19(2), 132–139 (2002).
   PubMed Web of Science ®Google Scholar
• Doe B, Selby M, Barnett S, Baenziger J, Walker CM. Induction of cytotoxic T lymphocytes by intramuscular immunization with plasmid DNA is facilitated by bone marrow-derived cells. Proc. Natl Acad. Sci. USA16, 8578–8583 (1996).
   Google Scholar
• Zinkernagel RM, Ehl S, Aichele P, Oehen S, Kündig T, Hengartner H. Antigen localisation regulates immune responses in a dose- and time-dependent fashion: a geographical view of immune reactivity. Immunol. Rev.156(1), 199–209 (1997).
   PubMed Web of Science ®Google Scholar
• Bramwell VW, Perrie Y. Particulate delivery systems for vaccines: what can we expect? J. Pharm. Pharmacol.58, 717–728 (2006).
   PubMed Web of Science ®Google Scholar
• Brewer JM, Pollock KGJ, Tetley L, Russell DG. Vesicle size influences the trafficking, processing, and presentation of antigens in lipid vesicles. J. Immunol.173(10), 6143–6150 (2004).
   PubMed Web of Science ®Google Scholar
• Mottram PL, Leong D, Crimeen-Irwin B et al. Type 1 and 2 immunity following vaccination is influenced by nanoparticle size: formulation of a model vaccine for respiratory syncytial virus. Mol. Pharm.4(1), 73–84 (2006).
   Web of Science ®Google Scholar
• Audran R, Peter K, Dannull J et al. Encapsulation of peptides in biodegradable microspheres prolongs their MHC class-I presentation by dendritic cells and macrophages in vitro. Vaccine21(11–12), 1250–1255 (2003).
   PubMed Web of Science ®Google Scholar
• Balasse E, Odot J, Gatouillat G, Andry M-C, Madoulet C. Enhanced immune response induced by BSA loaded in hydroxyethylstarch microparticles. Int. J. Pharm.353(1–2), 131–138 (2008).
   PubMed Web of Science ®Google Scholar
• Reddy ST, van der Vlies AJ, Simeoni E et al. Exploiting lymphatic transport and complement activation in nanoparticle vaccines. Nat. Biotech.25(10), 1159–1164 (2007).
   PubMed Web of Science ®Google Scholar
• Manolova V, Flace A, Bauer M, Schwarz K, Saudan P, Bachmann Martin F. Nanoparticles target distinct dendritic cell populations according to their size. Eur. J. Immunol.38(5), 1404–1413 (2008).
   PubMed Web of Science ®Google Scholar
• Mutwiri G, Benjamin P, Soita H, Babiuk LA. Co-administration of polyphosphazenes with CpG oligodeoxynucleotides strongly enhances immune responses in mice immunized with Hepatitis B virus surface antigen. Vaccine26(22), 2680–2688 (2008).
   PubMed Web of Science ®Google Scholar
• Briones M, Singh M, Ugozzoli M et al. The preparation, characterization, and evaluation of cationic microparticles for DNA vaccine delivery. Pharm. Res.18(5), 709–712 (2001).
   PubMed Web of Science ®Google Scholar
• O’Hagan D, Singh M, Ugozzoli M et al. Induction of potent immune responses by cationic microparticles with adsorbed human immunodeficiency virus DNA vaccines. J. Virol.75(19), 9037–9043 (2001).
   PubMed Web of Science ®Google Scholar
• Borges O, Borchard G, Verhoef JC, de Sousa A, Junginger HE. Preparation of coated nanoparticles for a new mucosal vaccine delivery system. Int. J. Pharm.299(1–2), 155–166 (2005).
   PubMed Web of Science ®Google Scholar
• Foged C, Brodin B, Frokjaer S, Sundblad A. Particle size and surface charge affect particle uptake by human dendritic cells in an in vitro model. Int. J. Pharm.298(2), 315–322 (2005).
   PubMed Web of Science ®Google Scholar
• Neutra MR, Kozlowski PA. Mucosal vaccines: the promise and the challenge. Nat. Rev. Immunol.6(2), 148–158 (2006).
   PubMed Web of Science ®Google Scholar
• Nakaoka R, Inoue Y, Tabata Y, Ikada Y. Size effect on the antibody production induced by biodegradable microspheres containing antigen. Vaccine14(13), 1251–1256 (1996).
   PubMed Web of Science ®Google Scholar
• Mohammed AR, Bramwell VW, Coombes AGA, Perrie Y. Lyophilisation and sterilisation of liposomal vaccines to produce stable and sterile products. Methods40(1), 30–38 (2006).
   PubMed Web of Science ®Google Scholar
• Memisoglu-Bilensoy E, Hincal AA. Sterile, injectable cyclodextrin nanoparticles: Effects of γ irradiation and autoclaving. Int. J. Pharm.311(1–2), 203–208 (2006).
   PubMed Web of Science ®Google Scholar
• Konan YN, Cerny R, Favet J, Berton M, Gurny R, Allémann E. Preparation and characterization of sterile sub-200 nm meso-tetra(4-hydroxylphenyl)porphyrin-loaded nanoparticles for photodynamic therapy. Eur. J. Pharm. Biopharm.55(1), 115–124 (2003).
   PubMed Web of Science ®Google Scholar
• Konan YN, Gurny R, Allémann E. Preparation and characterization of sterile and freeze-dried sub-200 nm nanoparticles. Int. J. Pharm.233(1–2), 239–252 (2002).
   PubMed Web of Science ®Google Scholar
• Masson V, Maurin F, Fessi H, Devissaguet JP. Influence of sterilization processes on poly([var e]-caprolactone) nanospheres. Biomaterials18(4), 327–335 (1997).
   PubMed Web of Science ®Google Scholar
• Sintzel MB, Merkli A, Tabatabay C, Gurny R. Influence of irradiation sterilization on polymers used as drug carriers: a review. Drug Develop. Industrial Pharm.23(9), 857–878 (1997).
   Web of Science ®Google Scholar
• Mehnert W, Mäder K. Solid lipid nanoparticles: production, characterization and applications. Adv. Drug Deliv. Rev.47(2–3), 165–196 (2001).
   PubMed Web of Science ®Google Scholar
• Rollot JM, Couvreur P, Roblot-Treupel L, Puisieux F. Physicochemical and morphological characterization of polyisobutyl cyanoacrylate nanocapsules. J. Pharm. Sci.75(4), 361–364 (1986).
   PubMed Web of Science ®Google Scholar
• Bozdag S, Dillen K, Vandervoort J, Ludwig A. The effect of freeze-drying with different cryoprotectants and γ-irradiation sterilization on the characteristics of ciprofloxacin HCl-loaded poly(D,L-lactide-glycolide) nanoparticles. J. Pharm. Pharmacol.57, 699–708 (2005).
   PubMed Web of Science ®Google Scholar
• Quintanar-Guerrero D, Ganem-Quintanar A, Allemann E, Fessi H, Doelker E. Influence of the stabilizer coating layer on the purification and freeze-drying of poly(D,L-lactic acid) nanoparticles prepared by an emulsion-diffusion technique. J. Microencapsul.15(1), 107–119 (1998).
   PubMed Web of Science ®Google Scholar
• Glavas-Dodov M, Fredro-Kumbaradzi E, Goracinova K et al. The effects of lyophilization on the stability of liposomes containing 5-FU. Int. J. Pharm.291(1–2), 79–86 (2005).
   PubMed Web of Science ®Google Scholar
• Chen D, Kristensen D. Opportunities and challenges of developing thermostable vaccines. Expert Rev. Vaccines8, 547–557 (2009).
   PubMed Web of Science ®Google Scholar
• Sloat BS, Sandoval MA, Cui Z. Towards preserving the immunogenicity of protein antigens carried by nanoparticles while avoiding the cold chain. Int. J. Pharm.393(1–2), 197–202 (2010).
   PubMed Web of Science ®Google Scholar
• Nixon DF, Hioe C, Chen P-D et al. Synthetic peptides entrapped in microparticles can elicit cytotoxic T cell activity. Vaccine14(16), 1523–1530 (1996).
   PubMed Web of Science ®Google Scholar
• Nagamoto T, Hattori Y, Takayama K, Maitani Y. Novel chitosan particles and chitosan-coated emulsions inducing immune response via intranasal vaccine delivery. Pharm. Res.21(4), 671–674 (2004).
   PubMed Web of Science ®Google Scholar
• Igartua M, Hernández RM, Esquisabel A, Gascón AR, Calvo MB, Pedraz JL. Enhanced immune response after subcutaneous and oral immunization with biodegradable PLGA microspheres. J. Controlled Release56(1–3), 63–73 (1998).
   PubMed Web of Science ®Google Scholar