Advanced search
Publication Cover
BioTechniques Volume 49, 2010 - Issue 1
Submit an article Journal homepage
3,855
Views
51
CrossRef citations to date
0
Altmetric
Reports

Design of an in vivo Cleavable Disulfide Linker in Recombinant Fusion Proteins

Xiaoying Chen1 Department of Pharmacology and Pharmaceutical Sciences,University of Southern California School of Pharmacy,Los Angeles, CA,USA
,
Yun Bai1 Department of Pharmacology and Pharmaceutical Sciences,University of Southern California School of Pharmacy,Los Angeles, CA,USA
,
Jennica L. Zaro1 Department of Pharmacology and Pharmaceutical Sciences,University of Southern California School of Pharmacy,Los Angeles, CA,USA
&
Wei-Chiang Shen1 Department of Pharmacology and Pharmaceutical Sciences,University of Southern California School of Pharmacy,Los Angeles, CA,USACorrespondence[email protected]
Pages 513-518 | Received 01 Apr 2010, Accepted 17 May 2010, Published online: 03 Apr 2018

References

  • Uhlen, M., G.Forsberg, T.Moks, M.Hartmanis, and B.Nilsson. 1992. Fusion proteins in biotechnology. Curr. Opin. Biotechnol.3:363–369.
  • Amet, N., H.F.Lee, and W.C.Shen. 2009. Insertion of the designed helical linker led to increased expression of tf-based fusion proteins. Pharm. Res.26:523–528.
  • Bai, Y. and W.C.Shen. 2006. Improving the oral efficacy of recombinant granulocyte colony-stimulating factor and transferrin fusion protein by spacer optimization. Pharm. Res.23:2116–2121.
  • Wriggers, W., S.Chakravarty, and P.A.Jennings. 2005. Control of protein functional dynamics by peptide linkers. Biopolymers80:736–746.
  • Arai, R., H.Ueda, A.Kitayama, N.Kamiya, and T.Nagamune. 2001. Design of the linkers which effectively separate domains of a bifunctional fusion protein. Protein Eng.14:529–532.
  • Yao, Z., W.Dai, J.Perry, M.W.Brechbiel, and C.Sung. 2004. Effect of albumin fusion on the biodistribution of interleukin-2. Cancer Immunol. Immunother.53:404–410.
  • Shen, W.C. 1999. Emerging targeting concepts membrane-associated protein thiol-disulfide interchange activity: a potential target for antiviral and anti-tumor drug design. J. Drug Target.6:387–389.
  • Shah, D. and W.C.Shen. 1996. Transcellular delivery of an insulintransferrin conjugate in enterocyte-like Caco-2 cells. J. Pharm. Sci.85:1306–1311.
  • Widera, A.S., K.-J.Kim, E.D.Crandall, and W.-C.Shen. 2003. Transcytosis of GCSF-transferrin across rat alveolar epithelial cell monolayers. Pharm. Res.20:1231–1238.
  • Widera, A., Y.Bai, and W.C.Shen. 2004. The transepithelial transport of a G-CSF-transferrin conjugate in caco-2 cells and its myelopoietic effect in BDF1 mice. Pharm. Res.21:278–284.
  • Bai, Y., D.K.Ann, and W.C.Shen. 2005. Recombinant granulocyte colony-stimulating factor-transferrin fusion protein as an oral myelopoietic agent. Proc. Natl. Acad. Sci. USA102:7292–7296.
  • Aricescu, A.R., W.Lu, and E.Y.Jones. 2006. A time- and cost-efficient system for high-level protein production in mammalian cells. Acta Crystallogr. D Biol. Crystallogr.62:1243–1250.
  • Hem, A., A.J.Smith, and P.Solberg. 1998. Saphenous vein puncture for blood sampling of the mouse, rat, hamster, gerbil, guinea pig, ferret and mink. Lab. Anim.32:364–368.
  • Amet, N., W.Wang, and W.C.Shen. 2010. Human growth hormonetransferrin fusion protein for oral delivery in hypophysectomized rats. J. Control. Release141:177–182.
  • Tamada, T., E.Honjo, Y.Maeda, T.Okamoto, M.Ishibashi, M.Tokunaga, and R.Kuroki. 2006. Homodimeric cross-over structure of the human granulocyte colony-stimulating factor (GCSF) receptor signaling complex. Proc. Natl. Acad. Sci. USA103:3135–3140.
  • Saito, G., J.A.Swanson, and K.D.Lee. 2003. Drug delivery strategy utilizing conjugation via reversible disulfide linkages: role and site of cellular reducing activities. Adv. Drug Deliv. Rev.55:199–215.
  • Thorpe, P.E., P.M.Wallace, P.P.Knowles, M.G.Relf, A.N.Brown, G.J.Watson, R.E.Knyba, E.J.Wawrzynczak, and D.C.Blakey. 1987. New coupling agents for the synthesis of immunotoxins containing a hindered disulfide bond with improved stability in vivo. Cancer Res.47:5924–5931.
  • Letvin, N.L., V.S.Goldmacher, J.Ritz, J.M.Yetz, S.F.Schlossman, and J.M.Lambert. 1986. In vivo administration of lymphocytespecific monoclonal antibodies in nonhuman primates. In vivo stability of disulfide-linked immunotoxin conjugates. J. Clin. Invest.77:977–984.
  • Arpicco, S., F.Dosio, P.Brusa, P.Crosasso, and L.Cattel. 1997. New coupling reagents for the preparation of disulfide cross-linked conjugates with increased stability. Bioconjug. Chem.8:327–337.
  • Xia, C.Q., J.Wang, and W.C.Shen. 2000. Hypoglycemic effect of insulin-transferrin conjugate in streptozotocin-induced diabetic rats. J. Pharmacol. Exp. Ther.295:594–600.
  • DeLeve, L.D. and N.Kaplowitz. 1991. Glutathione metabolism and its role in hepatotoxicity. Pharmacol. Ther.52:287–305.
  • Noiva, R. 1999. Protein disulfide isomerase: the multifunctional redox chaperone of the endoplasmic reticulum. Semin. Cell Dev. Biol.10:481–493.
  • Mandel, R., H.J.Ryser, F.Ghani, M.Wu, and D.Peak. 1993. Inhibition of a reductive function of the plasma membrane by bacitracin and antibodies against protein disulfide-isomerase. Proc. Natl. Acad. Sci. USA90:4112–4116.
QR code for Honorbridge. Scan the QR code or visit https://partners.honorbridge.org/research/ .
QR code for Honorbridge: We might have what your research needs. Click the QR code or visit https://partners.honorbridge.org/research/ to learn more.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.