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BioTechniques Volume 49, 2010 - Issue 1
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Fluorogenic “click” Reaction for Labeling and Detection of DNA in Proliferating Cells

Kai Li1 State Key Laboratory of Fine Chemicals,Dalian University of Technology (DUT),Dalian,China;2 Department of Chemistry and Biochemistry and Nanocenter,University of South Carolina,Columbia, SC,USAView further author information
,
L. Andrew Lee2 Department of Chemistry and Biochemistry and Nanocenter,University of South Carolina,Columbia, SC,USAView further author information
,
Xiaobing Lu1 State Key Laboratory of Fine Chemicals,Dalian University of Technology (DUT),Dalian,ChinaView further author information
&
Qian Wang2 Department of Chemistry and Biochemistry and Nanocenter,University of South Carolina,Columbia, SC,USACorrespondence[email protected]
View further author information
Pages 525-527 | Received 26 Oct 2009, Accepted 28 May 2010, Published online: 03 Apr 2018

References

  • Leif, R.C., J.H.Stein, and R.M.Zucker. 2004. A short history of the initial application of anti-5-BrdU to the detection and measurement of S phase. Cytometry A58:45–52.
  • Chehrehasa, F., A.C.B.Meedeniya, P.Dwyer, G.Abrahamsen, and A.MackaySim. 2009. EdU, a new thymidine analogue for labelling proliferating cells in the nervous system. J. Neurosci. Methods177:122–130.
  • Diermeier-Daucher, S., S.T.Clarke, D.Hill, A.Vollmann-Zwerenz, J.A.Bradford, and G.Brockhoff. 2009. Cell type specific applicability of 5-ethynyl-2′-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry. Cytometry A75:535–546.
  • Salic, A. and T.J.Mitchison. 2008. A chemical method for fast and sensitive detection of DNA synthesis in vivo. Proc. Natl. Acad. Sci. USA105:2415–2420.
  • Buck, S.B., J.Bradford, K.R.Gee, B.J.Agnew, S.T.Clarke, and A.Salic. 2008. Detection of S-phase cell cycle progression using 5-ethynyl 2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies. BioTechniques44:927–929.
  • Jao, C.Y. and A.Salic. 2008. Exploring RNA transcription and turnover in vivo by using click chemistry. Proc. Natl. Acad. Sci. USA105:15779–15784.
  • Rostovtsev, V.V., L.G.Green, V.V.Fokin, and K.B.Sharpless. 2002. A stepwise Huisgen cycloaddition process: copper (I)-catalyzed regioselective “ligation” of azides and terminal alkynes. Angew. Chem. Int. Ed. Engl.41:2596–2599.
  • Sivakumar, K., F.Xie, B.M.Cash, S.Long, H.N.Barnhill, and Q.Wang. 2004. A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org. Lett.6:4603–4606.
  • Beatty, K.E., J.C.Liu, F.Xie, D.C.Dieterich, E.M.Schuman, Q.Wang, and D.A.Tirrell. 2006. Fluorescence visualization of newly synthesized proteins in mammalian cells. Angew. Chem. Int. Ed. Engl.45:7364–7367.
  • Beatty, K.E., F.Xie, Q.Wang, and D.A.Tirrell. 2005. Selective dye-labeling of newly synthesized proteins in bacterial cells. J. Am. Chem. Soc.127:14150–14151.
  • Meneni, S., I.Ott, C.D.Sergeant, A.Sniady, R.Gust, and R.Dembinski. 2007. 5-Alkynyl-2′-deoxyuridines: chromatography-free synthesis and cytotoxicity evaluation against human breast cancer cells. Bioorg. Med. Chem.15:3082–3088.
  • Seela, F., V.R.Sirivolu, and P.Chittepu. 2008. Modification of DNA with octadiynyl side chains: Synthesis, base pairing, and formation of fluorescent coumarin dye conjugates of four nucleobases by the alkyne-azide “click” reaction. Bioconjug. Chem.19:211–224.
  • Le Droumaguet, C., C.Wang, and Q.Wang. 2010. Fluorogenic click reaction. Chem. Soc. Rev.39:1233–1239.
  • Xie, F., K.Siva kumar, Q.Zeng, M.A.Bruckman, B.Hodges, and Q.Wang. 2008. A fluorogenic ‘click’ reaction of azidoanthracene derivatives. Tetrahedron64:2906–2914.
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