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Establishing Isogenic Inducible Cell Lines Using Founder Reporter Lines and Recombinase-Mediated Cassette Exchange

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Pages 233-242 | Received 09 Mar 2013, Accepted 01 Jul 2013, Published online: 03 Apr 2018

References

  • Markstein, M., C.Pitsouli, C.Villalta, S.E.Celniker, and N.Perrimon. 2008. Exploiting position effects and the gypsy retrovirus insulator to engineer precisely expressed transgenes. Nat. Genet.40:476–483.
  • Meerbrey, K.L., G.Hu, J.D.Kessler, K.Roarty, M.Z.Li, J.E.Fang, J.I.Herschkowitz, A.E.Burrows, et al.. 2011. The pINDUCER lentiviral toolkit for inducible RNA interference in vitro and in vivo. Proc. Natl. Acad. Sci. USA108:3665–3670.
  • Weidenfeld, I., M.Gossen, R.Low, D.Kentner, S.Berger, D.Gorlich, D.Bartsch, H.Bujard, and K.Schonig. 2009. Inducible expression of coding and inhibitory RNAs from retargetable genomic loci. Nucleic Acids Res.37:e50.
  • Wong, E.T., J.L.Kolman, Y.C.Li, L.D.Mesner, W.Hillen, C.Berens, and G.M.Wahl. 2005. Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange. Nucleic Acids Res.33:e147.
  • Khandelia, P., K.Yap, and E.V.Makeyev. 2011. Streamlined platform for short hairpin RNA interference and transgenesis in cultured mammalian cells. Proc. Natl. Acad. Sci. USA108:12799–12804.
  • Schlake, T. and J.Bode. 1994. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry33:12746–12751.
  • Cheon, D.J. and S.Orsulic. 2011. Mouse models of cancer. Annu. Rev. Pathol.6:95–119.
  • McMullin, R.P., L.N.Mutton, and C.J.Bieberich. 2009. Hoxb13 regulatory elements mediate transgene expression during prostate organogenesis and carcinogenesis. Dev. Dyn.238:664–672.
  • Soyal, S.M., A.Mukherjee, K.Y.S.Lee, J.Li, H.G.Li, F.J.DeMayo, and J.P.Lydon. 2005. Cre-mediated recombination in cell lineages that express the progesterone receptor. Genesis41:58–66.
  • Campeau, E., V.E.Ruhl, F.Rodier, C.L.Smith, B.L.Rahmberg, J.O.Fuss, J.Campisi, P.Yaswen, et al.. 2009. A versatile viral system for expression and depletion of proteins in mammalian cells. PLoS ONE4:e6529.
  • Nam, H.S. and R.Benezra. 2009. High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell5:515–526.
  • Anderson, R.P., E.Voziyanova, and Y.Voziyanov. 2012. Flp and Cre expressed from Flp-2A-Cre and Flp-IRES-Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange. Nucleic Acids Res.40:e62.
  • Guan, B., T.L.Wang, and M.Shih Ie. 2011. ARID1A, a factor that promotes formation of SWI/SNF-mediated chromatin remodeling, is a tumor suppressor in gynecologic cancers. Cancer Res.71:6718–6727.
  • Barry, S.C., B.Harder, M.Brzezinski, L.Y.Flint, J.Seppen, and W.R.Osborne. 2001. Lentivirus vectors encoding both central polypurine tract and posttranscriptional regulatory element provide enhanced transduction and transgene expression. Hum. Gene Ther.12:1103–1108.
  • Brough, R., A.M.Papanastasiou, and A.C.Porter. 2007. Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics. BMC Mol. Biol.8:30.
  • Turan, S., C.Zehe, J.Kuehle, J.Qiao, and J.Bode. 2013. Recombinase-mediated cassette exchange (RMCE) - a rapidly-expanding toolbox for targeted genomic modifications. Gene515:1–27.