Advanced search
Publication Cover
BioTechniques Volume 55, 2013 - Issue 6
Submit an article Journal homepage
4,876
Views
39
CrossRef citations to date
0
Altmetric
Benchmarks

A Method for High-Throughput PCR-Based Genotyping of Larval Zebrafish Tail Biopsies

Robert N. Wilkinson1 Department of Cardiovascular Science,University of Sheffield,Medical School,Sheffield,United KingdomCorrespondence[email protected]
View further author information
,
Stone Elworthy2 Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics,University of Sheffield,Sheffield,United KingdomView further author information
,
Philip W. Ingham2 Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics,University of Sheffield,Sheffield,United KingdomView further author information
&
Fredericus J.M. van Eeden2 Department of Biomedical Science/ MRC Centre for Developmental and Biomedical Genetics,University of Sheffield,Sheffield,United KingdomView further author information
Pages 314-316 | Received 21 Aug 2013, Accepted 22 Oct 2013, Published online: 03 Apr 2018

References

  • Bedell, V.M., Y.Wang, J.M.Campbell, T.L.Poshusta, C.G.Starker, R.G.Krug, 2nd, W.Tan, S.G.Penheiter, et al.. 2012. In vivo genome editing using a high-efficiency TALEN system. Nature491:114–118.
  • Sander, J.D., L.Cade, C.Khayter, D.Reyon, R.T.Peterson, J.K.Joung, and J.R.Yeh. 2011. Targeted gene disruption in somatic zebrafish cells using engineered TALENs. Nat. Biotechnol.29:697–698.
  • Zu, Y., X.Tong, Z.Wang, D.Liu, R.Pan, Z.Li, Y.Hu, Z.Luo, et al.. 2013. TALEN-mediated precise genome modification by homologous recombination in zebrafish. Nat. Methods10:329–331.
  • Hwang, W.Y., Y.Fu, D.Reyon, M.L.Maeder, S.Q.Tsai, J.D.Sander, R.T.Peterson, J.R.Yeh, and J.K.Joung. 2013. Efficient genome editing in zebrafish using a CRISPR-Cas system. Nat. Biotechnol.31:227–229.
  • Kawakami, K. and N.Hopkins. 1996. Rapid identification of transgenic zebrafish. Trends Genet.12:9–10.
  • Westerfield, M. 1993. The zebrafish book. A guide for the laboratory use of zebrafish (Danio rerio). Univ. of Oregon Press, Eugene.
  • Meeker, N.D., S.A.Hutchinson, L.Ho, and N.S.Trede. 2007. Method for isolation of PCR-ready genomic DNA from zebrafish tissues. Biotechniques43:610–614.
  • van Rooijen, E., E.E.Voest, I.Logister, J.Korving, T.Schwerte, S.Schulte- Merker, R.H.Giles, and F.J.van Eeden. 2009. Zebrafish mutants in the von Hippel- Lindau tumor suppressor display a hypoxic response and recapitulate key aspects of Chuvash polycythemia. Blood113:6449–6460.
  • van Rooijen, E., E.E.Voest, I.Logister, J.Bussmann, J.Korving, F.J.van Eeden, R.H.Giles, and S.Schulte-Merker. 2010. von Hippel-Lindau tumor suppressor mutants faithfully model pathological hypoxia-driven angiogenesis and vascular retinopathies in zebrafish. Disease models & mechanisms3:343–353.
QR code for Honorbridge. Scan the QR code or visit https://partners.honorbridge.org/research/ .
QR code for Honorbridge: We might have what your research needs. Click the QR code or visit https://partners.honorbridge.org/research/ to learn more.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.