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BioTechniques Volume 38, 2005 - Issue 1
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Short Technical Reports

Combined sacB-Based Negative Selection and cre-lox Antibiotic Marker Recycling for Efficient Gene Deletion in Pseudomonas Aeruginosa

Lauriane Quénée1 Centre Hospitalier Universitaire de Grenoble,Grenoble,FranceView further author information
,
Danièle Lamotte1 Centre Hospitalier Universitaire de Grenoble,Grenoble,FranceView further author information
&
Benoît Polack1 Centre Hospitalier Universitaire de Grenoble,Grenoble,FranceCorrespondence[email protected]
View further author information
Pages 63-67 | Received 01 Jun 2004, Accepted 09 Aug 2004, Published online: 30 May 2018

References

  • Stover, C.K., X.Q.Pham, A.L.Erwin, S.D.Mizoguchi, P.Warrener, M.J.Hickey, F.S.L.Brinkman, W.O.Hufnagle, et al.. 2000. Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen. Nature406:959–964.
  • Banwart, B., M.L.Splaingard, P.M.Farrell, M.J.Rock, P.L.Havens, J.Moss, M.E.Ehrmantraut, D.W.Frank, et al.. 2002. Children with cystic fibrosis produce an immune response against exoenzyme S, a type III cytotoxin of Pseudomonas aeruginosa. J. Infect. Dis.185:269–270.
  • Moss, J., M.E.Ehrmantraut, B.D.Banwart, D.W.Frank, and J.T.Barbieri. 2001. Sera from adult patients with cystic fibrosis contain antibodies to Pseudomonas aeruginosa type III apparatus. Infect. Immun.69:1185–1188.
  • Roy-Burman, A., R.H.Savel, S.Racine, B.L.Swanson, N.S.Revadigar, J.Fujimoto, T.Sawa, D.W.Frank, et al.. 2001. Type III protein secretion is associated with death in lower respiratory and systemic Pseudomonas aeruginosa infections. J. Infect. Dis.183:1767–1774.
  • Schweizer, H.P. and T.T.Hoang. 1995. An improved system for gene replacement and xylE fusion analysis in Pseudomonas aeruginosa. Gene158:15–22.
  • Marx, C.J. and M.E.Lidstrom. 2002. Broad-host-range cre-lox system for antibiotic marker recycling in Gram-negative bacteria. BioTechniques33:1062–1067.
  • Palmeros, B., J.Wild, W.Szybalski, S.Le Borgne, G.Hernandez-Chavez, G.Gosset, F.Valle, and F.Bolivar. 2000. A family of removable cassettes designed to obtain antibiotic-resistance-free genomic modifications of Escherichia coli and other bacteria. Gene247:255–264.
  • Ayres, E.K., V.J.Thomson, G.Merino, D.Balderes, and D.H.Figurski. 1993. Precise deletions in large bacterial genomes by vector-mediated excision (VEX). The trfA gene of promiscuous plasmid RK2 is essential for replication in several Gram-negative hosts. J. Mol. Biol.230:174–185.
  • Sternberg, N. and D.Hamilton. 1981. Bacteriophage P1 site-specific recombination. I. Recombination between loxP sites. J. Mol. Biol.150:467–486.
  • Toussaint, B., I.Delic-Attree, and P.M.Vignais. 1993. Pseudomonas aeruginosa contains an IHF-like protein that binds to the algD promoter. Biochem. Biophys. Res. Commun.196:416–421.
  • Schweizer, H.D. 1993. Small broad-host-range gentamycin resistance gene cassettes for site-specific insertion and deletion mutagenesis. BioTechniques15:831–834.
  • Simon, R., U.Priefer, and A.Puhler. 1983. A broad rang mobilization system for in vivo genetic ingineering: transposon mutagenesis in Gram-negative bacteria. BioTechnology1:784–791.
  • Enderle, P.J. and M.A.Farwell. 1998. Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells. BioTechniques25:954–956.
  • Frank, D.W. 1997. The exoenzyme S regulon of Pseudomonas aeruginosa. Mol. Microbiol.26:621–629.
  • Wilson, J.W., D.H.Figurski, and C.A.Nickerson. 2004. VEX-capture: a new technique that allows in vivo excision, cloning, and broad-host-range transfer of large bacterial genomic DNA segments. J. Microbiol. Methods57:297–308.
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