Advanced search
Publication Cover
BioTechniques Volume 65, 2018 - Issue 4
Submit an article Journal homepage
2,390
Views
3
CrossRef citations to date
0
Altmetric
Benchmark

Mammalian cell culture density determination using a laser through-beam sensor

Sarah M Rue1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USACorrespondence[email protected]
,
Paul W Anderson1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA;2 Lilly Biotechnology Center,San Diego, CA 92121, USA
,
Jessica M Miller1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA
,
Salvatore G Fanale1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA
,
Jim Y Chang1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA
,
Scott M Glaser1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA
&
Scott A Lesley1 Genomics Institute of the Novartis Research Foundation,San Diego, CA 92121, USA;3 Merck Research Laboratories,Palo Alto, CA 94304, USA
 show all
Pages 224-226 | Received 25 Apr 2018, Accepted 07 Aug 2018, Published online: 04 Oct 2018

References

  • Koehn J , HuntI. High-throughput protein production (HTPP): a review of enabling technologies to expedite protein production. Methods Mol. Biol.498, 1–18 (2009).
  • Battle T , AntonssonB, FegerG, BessonD. A high-throughput mammalian protein expression, purification, aliquoting and storage pipeline to assemble a library of the human secretome. Comb. Chem. High Throughput Screen.9(9), 639–649 (2006).
  • Rue SM , AndersonPW, GaylordMR, MillerJM, GlaserSM, LesleySA. A high-throughput system for transient and stable protein production in mammalian cells. In: Methods in Molecular Biology. VincentelliR (Ed.). (2018).
  • Louis KS , SiegelAC. Cell viability analysis using trypan blue: manual and automated methods. Methods Mol. Biol.740, 7–12 (2011).
  • Myers JA , CurtisBS, CurtisWR. Improving accuracy of cell and chromophore concentration measurements using optical density. BMC Biophysics6(1), 4 (2013).
  • Brescia P , RaymondK, BanksP. Monitoring cell growth in 2 μl volumes using a microplate reader (2011). www.biotek.com/resources/application-notes/monitoring-cell-growth-in-2-l-volumes-using-a-microplate-reader/.
  • Ude C , Schmidt-HagerJ, FindeisM, JohnGT, ScheperT, BeutelS. Application of an online-biomass sensor in an optical multisensory platform prototype for growth monitoring of biotechnical relevant microorganism and cell lines in single-use shake flasks. Sensors14(9), 17390–17405 (2014).
  • Debreczeny MP , RomeroJ, PetersenE. US 2009/0075248 A1 (2009).
  • Allier C , BordyT, HervéLet al. Label-free cell viability assay using lens-free microscopy. International Society for Optics and Photonics10497, doi:10.1117/12/2289811 (2018).
QR code for Honorbridge. Scan the QR code or visit https://partners.honorbridge.org/research/ .
QR code for Honorbridge: We might have what your research needs. Click the QR code or visit https://partners.honorbridge.org/research/ to learn more.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.