Sputum Gene Expression Reveals Dysregulation of Mast Cells and Basophils in Eosinophilic COPD

References

• Lozano R, Naghavi M, Foreman K, et al. Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet. 2012;380(9859):2095–2128. doi:10.1016/S0140-6736(12)61728-0
   PubMed Web of Science ®Google Scholar
• Wedzicha JA, Brill SE, Allinson JP, Donaldson GC. Mechanisms and impact of the frequent exacerbator phenotype in chronic obstructive pulmonary disease. BMC Med. 2013;11:181. doi:10.1186/1741-7015-11-181
   PubMed Web of Science ®Google Scholar
• Barnes PJ. Inflammatory mechanisms in patients with chronic obstructive pulmonary disease. J Allergy Clin Immunol. 2016;138(1):16–27. doi:10.1016/j.jaci.2016.05.011
   PubMed Web of Science ®Google Scholar
• Negewo NA, McDonald VM, Baines KJ, et al. Peripheral blood eosinophils: a surrogate marker for airway eosinophilia in stable COPD. Int J Chron Obstruct Pulmon Dis. 2016;11:1495–1504. doi:10.2147/COPD.S100338
   PubMed Web of Science ®Google Scholar
• Hiles SA, Gibson PG, McDonald VM. Disease burden of eosinophilic airway disease: comparing severe asthma, COPD and asthma–COPD overlap. Respirology. 2021;26:52–61.
   PubMed Web of Science ®Google Scholar
• Kim VL, Coombs NA, Staples KJ, et al. Impact and associations of eosinophilic inflammation in COPD: analysis of the AERIS cohort. Eur Respir J. 2017;50(4):1700853. doi:10.1183/13993003.00853-2017
   PubMed Web of Science ®Google Scholar
• Proboszcz M, Mycroft K, Paplinska-Goryca M, et al. Relationship between Blood and Induced Sputum Eosinophils, Bronchial Hyperresponsiveness and Reversibility of Airway Obstruction in Mild-to-Moderate Chronic Obstructive Pulmonary Disease. Copd. 2019;16(5–6):354–361. doi:10.1080/15412555.2019.1675150
   PubMedGoogle Scholar
• Hastie AT, Martinez FJ, Curtis JL, et al. Association of sputum and blood eosinophil concentrations with clinical measures of COPD severity: an analysis of the SPIROMICS cohort. Lancet Respir Med. 2017;5(12):956–967. doi:10.1016/S2213-2600(17)30432-0
   PubMed Web of Science ®Google Scholar
• Vedel-Krogh S, Nielsen SF, Lange P, Vestbo J, Nordestgaard BG. Blood Eosinophils and Exacerbations in Chronic Obstructive Pulmonary Disease. The Copenhagen General Population Study. Am J Respir Crit Care Med. 2016;193(9):965–974. doi:10.1164/rccm.201509-1869OC
   PubMed Web of Science ®Google Scholar
• Ortega H, Llanos JP, Lafeuille MH, et al. Burden of disease associated with a COPD eosinophilic phenotype. Int J Chron Obstruct Pulmon Dis. 2018;13:2425–2433. doi:10.2147/COPD.S170995
   PubMed Web of Science ®Google Scholar
• Bradding P, Arthur G. Mast cells in asthma – state of the art. Clin Exp Allergy. 2016;46(2):194–263.
   PubMed Web of Science ®Google Scholar
• Karasuyama H, Miyake K, Yoshikawa S, Yamanishi Y. Multifaceted roles of basophils in health and disease. J Allergy Clin Immunol. 2018;142(2):370–380. doi:10.1016/j.jaci.2017.10.042
   PubMed Web of Science ®Google Scholar
• Komi DEA, Mortaz E, Amani S, Tiotiu A, Folkerts G, Adcock IM. The Role of Mast Cells in IgE-Independent Lung Diseases. Clin Rev Allergy Immunol. 2020;58(3):377–387. doi:10.1007/s12016-020-08779-5
   PubMed Web of Science ®Google Scholar
• Fricker M, Qin L, Niessen N, et al. Relationship of sputum mast cells with clinical and inflammatory characteristics of asthma. Clin Exp Allergy. 2020;50(6):696–707. doi:10.1111/cea.13609
   PubMed Web of Science ®Google Scholar
• Kuo CS, Pavlidis S, Loza M, et al. T-helper cell type 2 (Th2) and non-Th2 molecular phenotypes of asthma using sputum transcriptomics in U-BIOPRED. Eur Respir J. 2017;49(2):1602135. doi:10.1183/13993003.02135-2016
   PubMed Web of Science ®Google Scholar
• Peters MC, Ringel L, Dyjack N, et al. A Transcriptomic Method to Determine Airway Immune Dysfunction in T2-High and T2-Low Asthma. Am J Respir Crit Care Med. 2019;199(4):465–477. doi:10.1164/rccm.201807-1291OC
   PubMed Web of Science ®Google Scholar
• Winter NA, Qin L, Gibson PG, et al. Sputum mast cell/basophil gene expression relates to inflammatory and clinical features of severe asthma. J Allergy Clin Immunol. 2021. doi:10.1016/j.jaci.2021.01.033
   Web of Science ®Google Scholar
• Ekberg-Jansson A, Amin K, Bake B, et al. Bronchial mucosal mast cells in asymptomatic smokers relation to structure, lung function and emphysema. Respir Med. 2005;99(1):75–83. doi:10.1016/j.rmed.2004.05.013
   PubMed Web of Science ®Google Scholar
• Gosman MM, Postma DS, Vonk JM, et al. Association of mast cells with lung function in chronic obstructive pulmonary disease. Respir Res. 2008;9:64. doi:10.1186/1465-9921-9-64
   PubMed Web of Science ®Google Scholar
• Wen Y, Reid DW, Zhang D, Ward C, Wood-Baker R, Walters EH. Assessment of airway inflammation using sputum, BAL, and endobronchial biopsies in current and ex-smokers with established COPD. Int J Chron Obstruct Pulmon Dis. 2010;5:327–334.
   PubMedGoogle Scholar
• Jogdand P, Mori M, Bergqvist A, Walls A, Sanden C, Erjefält J. Mapping of eosinophil and basophils in COPD lung tissues. Eur Respir J. 2015;46(suppl 59):PA384.
   Google Scholar
• Dwyer DF, Barrett NA, Austen KF. The Immunological Genome Project C. Expression profiling of constitutive mast cells reveals a unique identity within the immune system. Nat Immunol. 2016;17:878–887. doi:10.1038/ni.3445
   PubMed Web of Science ®Google Scholar
• Vieira Braga FA, Kar G, Berg M, et al. A cellular census of human lungs identifies novel cell states in health and in asthma. Nat Med. 2019;25(7):1153–1163. doi:10.1038/s41591-019-0468-5
   PubMed Web of Science ®Google Scholar
• Jiang J, Faiz A, Berg M, et al. Gene signatures from scRNA-seq accurately quantify mast cells in biopsies in asthma. Clin Exp Allergy. 2020;50:1428–1431. doi:10.1111/cea.13732
   PubMed Web of Science ®Google Scholar
• McDonald VM, Clark VL, Cordova-Rivera L, Wark PAB, Baines KJ, Gibson PG. Targeting treatable traits in severe asthma: a randomised controlled trial. Eur Respir J. 2020;55(3):1901509. doi:10.1183/13993003.01509-2019
   PubMed Web of Science ®Google Scholar
• Gibson PG, Wlodarczyk JW, Hensley MJ, et al. Epidemiological Association of Airway Inflammation with Asthma Symptoms and Airway Hyperresponsiveness in Childhood. Am J of Respir Crit Care Med. 1998;158(1):36–41. doi:10.1164/ajrccm.158.1.9705031
   PubMed Web of Science ®Google Scholar
• Simpson JL, Scott R, Boyle MJ, Gibson PG. Inflammatory subtypes in asthma: assessment and identification using induced sputum. Respirology. 2006;11(1):54–61. doi:10.1111/j.1440-1843.2006.00784.x
   PubMed Web of Science ®Google Scholar
• Baines KJ, Simpson JL, Wood LG, Scott RJ, Gibson PG. Transcriptional phenotypes of asthma defined by gene expression profiling of induced sputum samples. J Allergy Clin Immunol. 2011;127(1):153–160. e151–159. doi:10.1016/j.jaci.2010.10.024
   PubMed Web of Science ®Google Scholar
• Baines KJ, Simpson JL, Wood LG, et al. Sputum gene expression signature of 6 biomarkers discriminates asthma inflammatory phenotypes. J Allergy Clin Immunol. 2014;133(4):997–1007. doi:10.1016/j.jaci.2013.12.1091
   PubMed Web of Science ®Google Scholar
• Berthon BS, Gibson PG, Wood LG, MacDonald-Wicks LK, Baines KJ. A sputum gene expression signature predicts oral corticosteroid response in asthma. Eur Respir J. 2017;49(6):1700180. doi:10.1183/13993003.00180-2017
   PubMed Web of Science ®Google Scholar
• Peters MC, Mekonnen ZK, Yuan S, Bhakta NR, Woodruff PG, Fahy JV. Measures of gene expression in sputum cells can identify TH2-high and TH2-low subtypes of asthma. J Allergy Clin Immunol. 2014;133(2):388–394. doi:10.1016/j.jaci.2013.07.036
   PubMed Web of Science ®Google Scholar
• Krystel-Whittemore M, Dileepan KN, Wood JG. Mast Cell: a Multi-Functional Master Cell. Front Immunol. 2016;6:620. doi:10.3389/fimmu.2015.00620
   PubMed Web of Science ®Google Scholar
• Falkencrone S, Poulsen LK, Bindslev-Jensen C, et al. IgE-mediated basophil tumour necrosis factor alpha induces matrix metalloproteinase-9 from monocytes. Allergy. 2013;68(5):614–620. doi:10.1111/all.12143
   PubMed Web of Science ®Google Scholar
• Shibata S, Miyake K, Tateishi T, et al. Basophils trigger emphysema development in a murine model of COPD through IL-4–mediated generation of MMP-12–producing macrophages. Proc Natl Acad Sci U S A. 2018;115(51):13057–13062. doi:10.1073/pnas.1813927115
   PubMed Web of Science ®Google Scholar
• Miyake K, Karasuyama H. Emerging roles of basophils in allergic inflammation. Allergol Int. 2017;66(3):382–391. doi:10.1016/j.alit.2017.04.007
   PubMed Web of Science ®Google Scholar
• Wang G, Baines KJ, Fu JJ, et al. Sputum mast cell subtypes relate to eosinophilia and corticosteroid response in asthma. Eur Respir J. 2016;47(4):1123–1133. doi:10.1183/13993003.01098-2015
   PubMed Web of Science ®Google Scholar
• Singh D, Bassi M, Balzano D, et al. COPD patients with chronic bronchitis and higher sputum eosinophil counts show increased type-2 and PDE4 gene expression in sputum. J Cell Mol Med. 2021;25(2):905–918. doi:10.1111/jcmm.16146
   PubMed Web of Science ®Google Scholar
• Altman MC, Lai Y, Nolin JD, et al. Airway epithelium–shifted mast cell infiltration regulates asthmatic inflammation via IL-33 signaling. J Clin Invest. 2020;129(11):4979–4991. doi:10.1172/JCI126402
   Web of Science ®Google Scholar
• Roufosse F. Targeting the Interleukin-5 Pathway for Treatment of Eosinophilic Conditions Other than Asthma. Front Med. 2018;5:49. doi:10.3389/fmed.2018.00049
   PubMed Web of Science ®Google Scholar
• McLeod JJA, Baker B, Ryan JJ. Mast cell production and response to IL-4 and IL-13. Cytokine. 2015;75(1):57–61. doi:10.1016/j.cyto.2015.05.019
   PubMed Web of Science ®Google Scholar
• Borriello F, Longo M, Spinelli R, et al. IL-3 synergises with basophil-derived IL-4 and IL-13 to promote the alternative activation of human monocytes. Eur J Immunol. 2015;45(7):2042–2051. doi:10.1002/eji.201445303
   PubMed Web of Science ®Google Scholar
• Sarajlic M, Neuper T, Föhrenbach Quiroz KT, et al. IL-1β Induces SOCS2 Expression in Human Dendritic Cells. Int J Mol Sci. 2019;20:23. doi:10.3390/ijms20235931
   Web of Science ®Google Scholar
• Hu J, Winqvist O, Flores-Morales A, Wikström AC, Norstedt G. SOCS2 influences LPS induced human monocyte-derived dendritic cell maturation. PLoS One. 2009;4(9):e7178. doi:10.1371/journal.pone.0007178
   PubMed Web of Science ®Google Scholar
• Li Y, Qi X, Liu B, Huang H. The STAT5-GATA2 pathway is critical in basophil and mast cell differentiation and maintenance. J Immunol. 2015;194(9):4328–4338. doi:10.4049/jimmunol.1500018
   PubMed Web of Science ®Google Scholar
• Desai A, Sowerwine K, Liu Y, et al. GATA-2-deficient mast cells limit IgE-mediated immediate hypersensitivity reactions in human subjects. J Allergy Clin Immunol. 2019;144(2):613–617.e614. doi:10.1016/j.jaci.2019.05.007
   PubMed Web of Science ®Google Scholar
• Huang H, Li Y, Liang J, Finkelman FD. Molecular Regulation of Histamine Synthesis. Front Immunol. 2018;9:1392. doi:10.3389/fimmu.2018.01392
   PubMed Web of Science ®Google Scholar
• Cahill KN, Katz HR, Cui J, et al. KIT Inhibition by Imatinib in Patients with Severe Refractory Asthma. N Engl J Med. 2017;376(20):1911–1920. doi:10.1056/NEJMoa1613125
   PubMed Web of Science ®Google Scholar
• Baines KJ, Negewo NA, Gibson PG, et al. A Sputum 6 Gene Expression Signature Predicts Inflammatory Phenotypes and Future Exacerbations of COPD. Int J Chron Obstruct Pulmon Dis. 2020;15:1577–1590. doi:10.2147/COPD.S245519
   PubMed Web of Science ®Google Scholar
• Dougherty RH, Sidhu SS, Raman K, et al. Accumulation of intraepithelial mast cells with a unique protease phenotype in T(H)2-high asthma. J Allergy Clin Immunol. 2010;125(5):1046–1053.e1048. doi:10.1016/j.jaci.2010.03.003
   PubMed Web of Science ®Google Scholar
• Balzar S, Fajt ML, Comhair SA, et al. Mast cell phenotype, location, and activation in severe asthma. Data from the Severe Asthma Research Program. Am J Respir Crit Care Med. 2011;183(3):299–309. doi:10.1164/rccm.201002-0295OC
   PubMed Web of Science ®Google Scholar
• Inclan-Rico JM, Ponessa JJ, Valero-Pacheco N, et al. Basophils prime group 2 innate lymphoid cells for neuropeptide-mediated inhibition. Nat Immunol. 2020;21(10):1181–1193. doi:10.1038/s41590-020-0753-y
   PubMed Web of Science ®Google Scholar