In vitro reduction of colistin susceptibility and comparative genomics reveals multiple differences between MCR-positive and MCR-negative colistin-resistant Escherichia coli

References

• Poirel L, Jayol A, Nordmann P. Polymyxins: antibacterial activity, susceptibility testing, and resistance mechanisms encoded by plasmids or chromosomes. Clin Microbiol Rev. 2017;30(2):557–596. doi:10.1128/CMR.00064-1628275006
   PubMed Web of Science ®Google Scholar
• Liu YY, Wang Y, Walsh TR, et al. Emergence of plasmid-mediated colistin resistance mechanism MCR-1 in animals and human beings in China: a microbiological and molecular biological study. Lancet Infect Dis. 2016;16(2):161–168. doi:10.1016/S1473-3099(15)00424-726603172
   PubMed Web of Science ®Google Scholar
• Wang X, Liu Y, Qi X, et al. Molecular epidemiology of colistin-resistant Enterobacteriaceae in inpatient and avian isolates from China: high prevalence of mcr-negative Klebsiella pneumoniae. Int J Antimicrob Agents. 2017;50(4):536–541. doi:10.1016/j.ijantimicag.2017.05.00928668693
   PubMed Web of Science ®Google Scholar
• Cao L, Li X, Xu Y, Shen J. Prevalence and molecular characteristics of mcr-1 colistin resistance in Escherichia coli: isolates of clinical infection from a Chinese University Hospital. Infect Drug Resist. 2018;11:1597–1603. doi:10.2147/IDR.S16672630310295
   PubMed Web of Science ®Google Scholar
• Li Y, Lin X, Yao X, et al. Synergistic antimicrobial activity of colistin in combination with rifampin and azithromycin against Escherichia coli producing MCR-1. Antimicrob Agents Chemother. 2018;62(12):e01631–e01618. doi:10.1128/AAC.01631-18
   Web of Science ®Google Scholar
• Lee JY, Ko KS. Mutations and expression of PmrAB and PhoPQ related with colistin resistance in Pseudomonas aeruginosa clinical isolates. Diagn Microbiol Infect Dis. 2014;78(3):271–276. doi:10.1016/j.diagmicrobio.2013.11.02724412662
   PubMed Web of Science ®Google Scholar
• Thi Khanh Nhu N, Dw R, Do Hoang Nhu T, et al. The induction and identification of novel Colistin resistance mutations in Acinetobacter baumannii and their implications. Sci Rep. 2016;6:28291. doi:10.1038/srep2829127329501
   PubMed Web of Science ®Google Scholar
• Hua X, Liu L, Fang Y, et al. Colistin resistance in acinetobacter baumannii MDR-ZJ06 revealed by a multiomics approach. Front Cell Infect Micro. 2017;7:45. doi:10.3389/fcimb.2017.00045
   Web of Science ®Google Scholar
• Fernandez L, Alvarez-Ortega C, Wiegand I, et al. Characterization of the polymyxin B resistome of Pseudomonas aeruginosa. Antimicrob Agents Chemother. 2013;57(1):110–119. doi:10.1128/AAC.01583-1223070157
   PubMed Web of Science ®Google Scholar
• Quesada A, Porrero MC, Tellez S, Palomo G, Garcia M, Dominguez L. Polymorphism of genes encoding PmrAB in colistin-resistant strains of Escherichia coli and Salmonella enterica isolated from poultry and swine. J Antimicrob Chemother. 2015;70(1):71–74. doi:10.1093/jac/dku32025150146
   PubMed Web of Science ®Google Scholar
• Arena F, Henrici De Angelis L, Cannatelli A, et al. Colistin resistance caused by inactivation of the mgrb regulator is not associated with decreased virulence of sequence type 258 KPC carbapenemase-producing klebsiella pneumoniae. Antimicrob Agents Chemother. 2016;60(4):2509–2512. doi:10.1128/AAC.02981-1526824959
   PubMed Web of Science ®Google Scholar
• Phan MD, Nhu NTK, Achard MES, et al. Modifications in the pmrB gene are the primary mechanism for the development of chromosomally encoded resistance to polymyxins in uropathogenic Escherichia coli. J Antimicrob Chemother. 2017;72(10):2729–2736. doi:10.1093/jac/dkx20429091192
   PubMed Web of Science ®Google Scholar
• Luo Q, Yu W, Zhou K, et al. Molecular epidemiology and colistin resistant mechanism of MCR-Positive and MCR-Negative clinical isolated Escherichia Coli. Front Microbiol. 2017;8:2262.29250039
   PubMed Web of Science ®Google Scholar
• Zhou K, Luo Q, Wang Q, et al. Silent transmission of an IS1294b-deactivated mcr-1 gene with inducible colistin resistance. Int J Antimicrob Agents. 2018;51(6):822–828. doi:10.1016/j.ijantimicag.2018.01.00429339296
   PubMed Web of Science ®Google Scholar
• Rosche WA, Foster PL. Determining mutation rates in bacterial populations. Methods. 2000;20(1):4–17. doi:10.1006/meth.1999.090110610800
   PubMed Web of Science ®Google Scholar
• Deatherage DE, Barrick JE. Identification of mutations in laboratory-evolved microbes from next-generation sequencing data using breseq. Methods Mol Biol. 2014;1151:165–188. doi:10.1007/978-1-4939-0554-6_1224838886
   PubMedGoogle Scholar
• San Millan A, Toll-Riera M, Qi Q, MacLean RC. Interactions between horizontally acquired genes create a fitness cost in Pseudomonas aeruginosa. Nat Commun. 2015;6:6845. doi:10.1038/ncomms784525897488
   PubMed Web of Science ®Google Scholar
• Liu D, Liu ZS, Hu P, et al. Characterization of surface antigen protein 1 (SurA1) from Acinetobacter baumannii and its role in virulence and fitness. Vet Microbiol. 2016;186(15):126–138. doi:10.1016/j.vetmic.2016.02.01827016767
   PubMed Web of Science ®Google Scholar
• Bachman MA, Breen P, Deornellas V, et al. Genome-Wide Identification of Klebsiella pneumoniae Fitness Genes during Lung Infection. mBio. 2015;6(3):e00775. doi:10.1128/mBio.00775-1526060277
   PubMed Web of Science ®Google Scholar
• Garcia-Quintanilla M, Pulido MR, Moreno-Martinez P, et al. Activity of host antimicrobials against multidrug-resistant Acinetobacter baumannii acquiring colistin resistance through loss of lipopolysaccharide. Antimicrob Agents Chemother. 2014;58(5):2972–2975. doi:10.1128/AAC.02642-1324566189
   PubMed Web of Science ®Google Scholar
• Lin MF, Lin YY, Lan CY. Contribution of EmrAB efflux pumps to colistin resistance inAcinetobacter Baumannii. J Microbiol. 2017;55(2):130–136.28120193
   PubMed Web of Science ®Google Scholar
• Machado D, Antunes J, Simoes A, et al. Contribution of efflux to colistin heteroresistance in a multidrug resistant Acinetobacter baumannii clinical isolate. J Med Microbiol. 2018;67(6):740–749. doi:10.1099/jmm.0.00074129717972
   PubMed Web of Science ®Google Scholar
• Ni W, Li Y, Guan J, et al. Effects of efflux pump inhibitors on colistin resistance in multidrug-resistant gram-negative bacteria. Antimicrob Agents Chemother. 2016;60(5):3215–3218. doi:10.1128/AAC.00248-1626953203
   PubMed Web of Science ®Google Scholar
• Poole K, Lau CH, Gilmour C, Hao Y, Lam JS. Polymyxin susceptibility in pseudomonas aeruginosa linked to the MexXY-OprM multidrug efflux system. Antimicrob Agents Chemother. 2015;59(12):7276–7289. doi:10.1128/AAC.01785-1526369970
   PubMed Web of Science ®Google Scholar
• Eguchi Y, Okada T, Minagawa S, et al. Signal transduction cascade between EvgA/EvgS and PhoP/PhoQ two-component systems of Escherichia Coli. J Bacteriol. 2004;186(10):3006–3014.15126461
   PubMed Web of Science ®Google Scholar
• Nishino K, Yamaguchi A. Overexpression of the response regulator evgA of the two-component signal transduction system modulates multidrug resistance conferred by multidrug resistance transporters. J Bacteriol. 2001;183(4):1455–1458. doi:10.1128/JB.183.4.1455-1458.200111157960
   PubMed Web of Science ®Google Scholar
• Velkov T, Deris ZZ, Huang JX, et al. Surface changes and polymyxin interactions with a resistant strain of Klebsiella Pneumoniae. Innate Immun. 2014;20(4):350–363. doi:10.1177/175342591349333723887184
   PubMed Web of Science ®Google Scholar
• Mu X, Wang N, Li X, et al. The effect of colistin resistance-associated mutations on the fitness of acinetobacter baumannii. Front Microbiol. 2016;7:1715. doi:10.3389/fmicb.2016.0171527847502
   PubMed Web of Science ®Google Scholar
• Zusman O, Altunin S, Koppel F, Dishon Benattar Y, Gedik H, Paul M. Polymyxin monotherapy or in combination against carbapenem-resistant bacteria: systematic review and meta-analysis. J Antimicrob Chemother. 2017;72(1):29–39. doi:10.1093/jac/dkw37727624572
   PubMed Web of Science ®Google Scholar
• Gregoire N, Aranzana-Climent V, Magreault S, Marchand S, Clinical pharmacokinetics CW. Pharmacodynamics of colistin. Clin Pharmacokinet. 2017;56(12):1441–1460. doi:10.1007/s40262-017-0561-128550595
   PubMed Web of Science ®Google Scholar