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Original Research

Simultaneous detection of eleven sexually transmitted agents using multiplexed PCR coupled with MALDI-TOF analysis

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Pages 2671-2682 | Published online: 28 Aug 2019

References

  • Unemo M, Bradshaw CS, Hocking JS, et al. Sexually transmitted infections: challenges ahead. Lancet Infect Dis. 2017;17(8):e235–e279. doi:10.1016/S1473-3099(17)30242-628701272
  • McKechnie ML, Hillman R, Couldwell D, et al. Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay. J Clin Microbiol. 2009;47(6):1871–1877. doi:10.1128/JCM.00120-0919357202
  • Cao B, Wang S, Tian Z, Hu P, Feng L, Wang L. DNA microarray characterization of pathogens associated with sexually transmitted diseases. PLoS One. 2015;10(7):e0133927. doi:10.1371/journal.pone.013392726208181
  • Gaydos C, Hardick J. Point of care diagnostics for sexually transmitted infections: perspectives and advances. Expert Rev Anti Infect Ther. 2014;12(6):657–672.24484215
  • Workowski KA, Bolan GA; Centers for Disease C, Prevention. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep. 2015;64(RR–03):1–137.
  • Karaer A, Mert I, Cavkaytar S, Batioglu S. Serological investigation of the role of selected sexually transmitted infections in the aetiology of ectopic pregnancy. Eur J Contracept Reprod Health Care. 2013;18(1):68–74.23256948
  • Trembizki E, Costa AM, Tabrizi SN, Whiley DM, Twin J. Opportunities and pitfalls of molecular testing for detecting sexually transmitted pathogens. Pathology. 2015;47(3):219–226.25714587
  • Van Dyck E, Ieven M, Pattyn S, Van Damme L, Laga M. Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by enzyme immunoassay, culture, and three nucleic acid amplification tests. J Clin Microbiol. 2001;39(5):1751–1756.11325985
  • Battle TJ, Golden MR, Suchland KL, et al. Evaluation of laboratory testing methods for Chlamydia trachomatis infection in the era of nucleic acid amplification. J Clin Microbiol. 2001;39(8):2924–2927.11474014
  • Wald A, Huang ML, Carrell D, Selke S, Corey L. Polymerase chain reaction for detection of herpes simplex virus (HSV) DNA on mucosal surfaces: comparison with HSV isolation in cell culture. J Infect Dis. 2003;188(9):1345–1351. doi:10.1086/37904314593592
  • Larsen SA, Steiner BM, Rudolph AH. Laboratory diagnosis and interpretation of tests for syphilis. Clin Microbiol Rev. 1995;8(1):1–21.7704889
  • Kriesel JD, Bhatia AS, Barrus C, Vaughn M, Gardner J, Crisp RJ. Multiplex PCR testing for nine different sexually transmitted infections. Int J STD AIDS. 2016;27(14):1275–1282. doi:10.1177/095646241561577526538551
  • Boel CH, van Herk CM, Berretty PJ, Onland GH, van Den Brule AJ. Evaluation of conventional and real-time PCR assays using two targets for confirmation of results of the COBAS AMPLICOR Chlamydia trachomatis/Neisseria gonorrhoeae test for detection of Neisseria gonorrhoeae in clinical samples. J Clin Microbiol. 2005;43(5):2231–2235. doi:10.1128/JCM.43.5.2231-2235.200515872247
  • Tabrizi SN, Unemo M, Golparian D, et al. Analytical evaluation of GeneXpert CT/NG, the first genetic point-of-care assay for simultaneous detection of Neisseria gonorrhoeae and Chlamydia trachomatis. J Clin Microbiol. 2013;51(6):1945–1947. doi:10.1128/JCM.00806-1323554203
  • Van Der Pol B, Ferrero DV, Buck-Barrington L, et al. Multicenter evaluation of the BDProbeTec ET system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs. J Clin Microbiol. 2001;39(3):1008–1016. doi:10.1128/JCM.39.3.1008-1016.200111230419
  • Song T, Ye A, Xie X, et al. Epidemiological investigation and antimicrobial susceptibility analysis of ureaplasma species and Mycoplasma hominis in outpatients with genital manifestations. J Clin Pathol. 2014;67(9):817–820. doi:10.1136/jclinpath-2014-20224824982440
  • Li K, Guo J, Zhao R, et al. Prevalence of 10 human polyomaviruses in fecal samples from children with acute gastroenteritis: a case-control study. J Clin Microbiol. 2013;51(9):3107–3109. doi:10.1128/JCM.01324-1323824769
  • Peng J, Gao L, Guo J, et al. Type-specific detection of 30 oncogenic human papillomaviruses by genotyping both E6 and L1 genes. J Clin Microbiol. 2013;51(2):402–408. doi:10.1128/JCM.01170-1223152557
  • Peng J, Li K, Zhang C, Gao L, Jin Q. Human papillomavirus and polyomavirus coinfections among Chinese men who have sex with men. J Infect. 2016;72(1):118–120. doi:10.1016/j.jinf.2015.09.01226416469
  • Peng J, Li K, Zhang C, Jin Q. MW polyomavirus and STL polyomavirus present in tonsillar tissues from children with chronic tonsillar disease. Clin Microbiol Infect. 2016;22(1):97e91–97e93. doi:10.1016/j.cmi.2015.08.028
  • Peng J, Yang F, Xiong Z, et al. Sensitive and rapid detection of viruses associated with hand foot and mouth disease using multiplexed MALDI-TOF analysis. J Clin Virol. 2013;56(2):170–174. doi:10.1016/j.jcv.2012.10.02023194776
  • Xiu L, Zhang C, Wu Z, Peng J. Establishment and application of a universal coronavirus screening method using MALDI-TOF mass spectrometry. Front Microbiol. 2017;8:1510. doi:10.3389/fmicb.2017.0151028848521
  • Zhang C, Xiao Y, Du J, et al. Application of multiplex PCR coupled with matrix-assisted laser desorption ionization-time of flight analysis for simultaneous detection of 21 common respiratory viruses. J Clin Microbiol. 2015;53(8):2549–2554. doi:10.1128/JCM.00943-1526019198
  • Zhang C, Xiu L, Xiao Y, Xie Z, Ren L, Peng J. Simultaneous detection of key bacterial pathogens related to pneumonia and meningitis using multiplex PCR coupled with mass spectrometry. Front Cell Infect Microbiol. 2018;8:107. doi:10.3389/fcimb.2018.0002629675400
  • Gao L, Zhou F, Li X, Yang Y, Ruan Y, Jin Q. Anal HPV infection in HIV-positive men who have sex with men from China. PLoS One. 2010;5(12):e15256. doi:10.1371/journal.pone.001525621151900
  • Kessler HH, Muhlbauer G, Rinner B, et al. Detection of Herpes simplex virus DNA by real-time PCR. J Clin Microbiol. 2000;38(7):2638–2642.10878056
  • Nicoll S, Brass A, Cubie HA. Detection of herpes viruses in clinical samples using real-time PCR. J Virol Methods. 2001;96(1):25–31.11516486
  • Jensen JS, Borre MB, Dohn B. Detection of Mycoplasma genitalium by PCR amplification of the 16S rRNA gene. J Clin Microbiol. 2003;41(1):261–266. doi:10.1128/jcm.41.1.261-266.200312517858
  • Del Prete R, Ronga L, Lestingi M, et al. Simultaneous detection and identification of STI pathogens by multiplex Real-Time PCR in genital tract specimens in a selected area of Apulia, a region of Southern Italy. Infection. 2017;45(4):469–477. doi:10.1007/s15010-017-1002-728260146
  • Schmitt M, Depuydt C, Stalpaert M, Pawlita M. Bead-based multiplex sexually transmitted infection profiling. J Infect. 2014;69(2):123–133. doi:10.1016/j.jinf.2014.04.00624814157
  • Cosentino LA, Danby CS, Rabe LK, et al. Use of nucleic acid amplification testing for diagnosis of extragenital sexually transmitted infections. J Clin Microbiol. 2017;55(9):2801–2807. doi:10.1128/JCM.00616-1728679521
  • Bercot B, Amarsy R, Goubard A, et al. Assessment of coinfection of sexually transmitted pathogen microbes by use of the anyplex II STI-7 molecular kit. J Clin Microbiol. 2015;53(3):991–993. doi:10.1128/JCM.03370-1425540390
  • Gaydos CA, Quinn TC, Willis D, et al. Performance of the APTIMA Combo 2 assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in female urine and endocervical swab specimens. J Clin Microbiol. 2003;41(1):304–309.12517865
  • Gaydos CA, Cartwright CP, Colaninno P, et al. Performance of the Abbott RealTime CT/NG for detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J Clin Microbiol. 2010;48(9):3236–3243.20668135
  • Lee SJ, Park DC, Lee DS, Choe HS, Cho YH. Evaluation of Seeplex(R) STD6 ACE detection kit for the diagnosis of six bacterial sexually transmitted infections. J Infect Chemother. 2012;18(4):494–500.22252268
  • Van Der Pol B. Profile of the triplex assay for detection of chlamydia, gonorrhea and trichomonas using the BD MAX System. Expert Rev Mol Diagn. 2017;17(6):539–547.28425774
  • Van Der Pol B, Williams JA, Fuller D, Taylor SN, Hook EW 3rd. Combined testing for chlamydia, gonorrhea, and trichomonas by use of the BD max CT/GC/TV assay with genitourinary specimen types. J Clin Microbiol. 2017;55(1):155–164.27795343
  • Tabrizi SN, Unemo M, Limnios AE, et al. Evaluation of six commercial nucleic acid amplification tests for detection of Neisseria gonorrhoeae and other Neisseria species. J Clin Microbiol. 2011;49(10):3610–3615.21813721
  • Jalal H, Stephen H, Curran MD, Burton J, Bradley M, Carne C. Development and validation of a rotor-gene real-time PCR assay for detection, identification, and quantification of Chlamydia trachomatis in a single reaction. J Clin Microbiol. 2006;44(1):206–213.16390971
  • O’Neil D, Doseeva V, Rothmann T, Wolff J, Nazarenko I. Evaluation of Chlamydia trachomatis and Neisseria gonorrhoeae detection in urine, endocervical, and vaginal specimens by a multiplexed isothermal thermophilic helicase-dependent amplification (tHDA) assay. J Clin Microbiol. 2011;49(12):4121–4125.21956990
  • Melendez JH, Huppert JS, Jett-Goheen M, et al. Blind evaluation of the microwave-accelerated metal-enhanced fluorescence ultrarapid and sensitive Chlamydia trachomatis test by use of clinical samples. J Clin Microbiol. 2013;51(9):2913–2920.23804384
  • Ripa T, Nilsson P. A variant of Chlamydia trachomatis with deletion in cryptic plasmid: implications for use of PCR diagnostic tests. Euro Surveill. 2006;11(11):E061109 061102.
  • Ripa T, Nilsson PA. A Chlamydia trachomatis strain with a 377-bp deletion in the cryptic plasmid causing false-negative nucleic acid amplification tests. Sex Transm Dis. 2007;34(5):255–256.17483723
  • Fieser N, Simnacher U, Tausch Y, et al. Chlamydia trachomatis prevalence, genotype distribution and identification of the new Swedish variant in Southern Germany. Infection. 2013;41(1):159–166.22855433
  • Seth-Smith HM, Thomson NR. Whole-genome sequencing of bacterial sexually transmitted infections: implications for clinicians. Curr Opin Infect Dis. 2013;26(1):90–98.23221768
  • Weerakoon KG, Gordon CA, Williams GM, et al. Droplet digital PCR diagnosis of human schistosomiasis: parasite cell-free DNA detection in diverse clinical samples. J Infect Dis. 2017;216(12):1611–1622.29029307
  • Blaya J, Lloret E, Santisima-Trinidad AB, Ros M, Pascual JA. Molecular methods (digital PCR and real-time PCR) for the quantification of low copy DNA of Phytophthora nicotianae in environmental samples. Pest Manag Sci. 2016;72(4):747–753.26012497
  • Belmonte FR, Martin JL, Frescura K, et al. Digital PCR methods improve detection sensitivity and measurement precision of low abundance mtDNA deletions. Sci Rep. 2016;6:25186.27122135
  • Kim EJ, Hladik W, Barker J, et al. Sexually transmitted infections associated with alcohol use and HIV infection among men who have sex with men in Kampala, Uganda. Sex Transm Infect. 2016;92(3):240–245.26424713
  • Choe HS, Lee DS, Lee SJ, et al. Performance of Anyplex II multiplex real-time PCR for the diagnosis of seven sexually transmitted infections: comparison with currently available methods. Int J Infect Dis. 2013;17(12):e1134–e1140.24095619
  • Zhu C, Liu J, Ling Y, et al. Prevalence and antimicrobial susceptibility of Ureaplasma urealyticum and Mycoplasma hominis in Chinese women with genital infectious diseases. Indian J Dermatol Venereol Leprol. 2012;78(3):406–407.