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ORIGINAL RESEARCH

Direct Identification, Antimicrobial Susceptibility Testing, and Extended-Spectrum β-Lactamase and Carbapenemase Detection in Gram-Negative Bacteria Isolated from Blood Cultures

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Pages 1587-1599 | Published online: 06 Apr 2022

References

  • McNamara JF, Righi E, Wright H, Hartel GF, Harris P, Paterson DL. Long-term morbidity and mortality following bloodstream infection: a systematic literature review. J Infect. 2018;77:1–8. doi:10.1016/j.jinf.2018.03.005
  • Kern WV, Rieg S. Burden of bacterial bloodstream infection-a brief update on epidemiology and significance of multidrug-resistant pathogens. Clin Microbiol Infect. 2020;26:151–157. doi:10.1016/j.cmi.2019.10.031
  • Hogan CA, Watz N, Budvytiene I, Banaei N. Rapid antimicrobial susceptibility testing by VITEK®2 directly from blood cultures in patients with Gram-negative rod bacteremia. Diagn Microbiol Infect Dis. 2019;94:116–121. doi:10.1016/j.diagmicrobio.2019.01.001
  • Kadri SS, Lai YL, Warner S, et al. Inappropriate empirical antibiotic therapy for bloodstream infections based on discordant in-vitro susceptibilities: a retrospective cohort analysis of prevalence, predictors, and mortality risk in US hospitals. Lancet Infect Dis. 2021;21(2):241–251. doi:10.1016/S1473-3099(20)30477-1
  • Magarifuchi H, Hamada Y, Oho M, Kusaba K, Urakami T, Aoki Y. Clinical utility of direct application of matrix-assisted laser desorption ionization time-of-flight mass spectrometry and rapid disk diffusion test in presumptive antimicrobial therapy for bacteremia. J Infect Chemother. 2018;24:881–886. doi:10.1016/j.jiac.2018.07.014
  • Peri AM, Harris PNA, Paterson DL. Culture-independent detection systems for bloodstream infection. Clin Microbiol Infect. 2022;28(2):195–201. doi:10.1016/j.cmi.2021.09.039
  • Johnsson A, Wong A, Özenci V. The impact of delayed analysis of positive blood cultures on the performance of short-term culture followed by MALDI-TOF MS. J Microbiol Methods. 2020;177:106027. doi:10.1016/j.mimet.2020.106027
  • Machen A, Drake T, Wang YF. Same day identification and full panel antimicrobial susceptibility testing of bacteria from positive blood culture bottles made possible by a combined lysis-filtration method with MALDI-TOF VITEK mass spectrometry and the VITEK2 system. PLoS One. 2014;9:e87870. doi:10.1371/journal.pone.0087870
  • Carretero O, Rivas G, Loras C, Orellana MA. Rapid identification of bacteria directly from positive blood cultures by a modified method using a serum separator tube and matrix-assisted laser desorption ionization- time of flight MS. J Med Microbiol. 2020;69:1373–1380. doi:10.1099/jmm.0.001270
  • Dai Y, Xu X, Yan X, et al. Evaluation of a rapid and simplified protocol for direct identification of microorganisms from positive blood cultures by using Matrix Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS). Front Cell Infect Microbiol. 2021;11:632679. doi:10.3389/fcimb.2021.632679
  • Romero-Gómez MP, Gómez-Gil R, Paño-Pardo JR, Mingorance J. Identification and susceptibility testing of microorganism by direct inoculation from positive blood culture bottles by combining MALDI-TOF and Vitek-2 compact is rapid and effective. J Infect. 2012;65:513–520. doi:10.1016/j.jinf.2012.08.013
  • Idelevich EA, Hoy M, Knaack D, et al. Direct determination of carbapenem-resistant Enterobacteriaceae and Pseudomonas aeruginosa from positive blood cultures using laser scattering technology. Int J Antimicrob Agents. 2018;51:221–226. doi:10.1016/j.ijantimicag.2017.10.009
  • Idelevich EA, Storck LM, Sparbier K, Drews O, Kostrzewa M, Becker K. Rapid direct susceptibility testing from positive blood cultures by the matrix-assisted laser desorption ionization-time of flight mass spectrometry-based direct-on-target microdroplet growth assay. J Clin Microbiol. 2018;56. doi:10.1128/JCM.00913-18
  • Prod’Hom G, Durussel C, Greub G. A simple blood-culture bacterial pellet preparation for faster accurate direct bacterial identification and antibiotic susceptibility testing with the VITEK 2 system. J Med Microbiol. 2013;62:773–777. doi:10.1099/jmm.0.049361-0
  • Idelevich EA, Becker K. How to accelerate antimicrobial susceptibility testing. Clin Microbiol Infect. 2019;25:1347–1355. doi:10.1016/j.cmi.2019.04.025
  • Shin J, Shina S, Jung SH, et al. Duplex dPCR system for rapid identification of Gram-negative pathogens in the blood of patients with bloodstream infection: a culture-independent approach. J Microbiol Biotechnol. 2021;31(11):1481–1489. doi:10.4014/jmb.2103.03044
  • Chalhoub H, Sáenz Y, Rodriguez-Villalobos H, et al. High-level resistance to meropenem in clinical isolates of Pseudomonas aeruginosa in the absence of carbapenemases: role of active efflux and porin alterations. Int J Antimicrob Agents. 2016;48:740–743. doi:10.1016/j.ijantimicag.2016.09.012
  • Culbreath K, Petti CA. Balancing enthusiasm for innovative technologies with optimizing value: an approach to adopt new laboratory tests for infectious diseases using bloodstream infections as exemplar. Open Forum Infect Dis. 2015;2:v75. doi:10.1093/ofid/ofv075
  • Idelevich EA, Becker K. Identification and susceptibility testing from shortly incubated cultures accelerate blood culture diagnostics at no cost. Clin Infect Dis. 2016;62:268–269. doi:10.1093/cid/civ824
  • Bard JD, Lee F. Why can’t we just use PCR? the role of genotypic versus phenotypic testing for antimicrobial resistance testing. Clin Microbiol Newsl. 2018;40:87–95. doi:10.1016/j.clinmicnews.2018.05.003
  • Adams-Sapper S, Nolen S, Donzelli GF, et al. Rapid induction of high-level carbapenem resistance in heteroresistant KPC-producing Klebsiella pneumoniae. Antimicrob Agents Chemother. 2015;59:3281–3289. doi:10.1128/AAC.05100-14
  • Jett B, Free L, Sahm DF. Factors influencing the Vitek Gram-positive susceptibility system’s detection of vanB-encoded vancomycin resistance among enterococci. J Clin Microbiol. 1996;34:701–706. doi:10.1128/jcm.34.3.701-706.1996
  • Anton-Vazquez V, Hine P, Krishna S, Chaplin M, Planche T. Rapid versus standard antimicrobial susceptibility testing to guide treatment of bloodstream infection. Cochrane Database Syst Rev. 2021;5(5):CD013235. doi:10.1002/14651858.CD013235.pub2
  • Giacobbe DR, Giani T, Bassetti M, Marchese A, Viscoli C, Rossolini GM. Rapid microbiological tests for bloodstream infections due to multidrug resistant Gram-negative bacteria: therapeutic implications. Clin Microbiol Infect. 2020;26:713–722. doi:10.1016/j.cmi.2019.09.023
  • Harris P, Tambyah PA, Lye DC, et al. Effect of piperacillin-tazobactam vs meropenem on 30-day mortality for patients with E. coli or Klebsiella pneumoniae bloodstream infection and ceftriaxone resistance: a randomized clinical trial. JAMA. 2018;320:984–994. doi:10.1001/jama.2018.12163
  • Bush K, Bradford PA. Epidemiology of β-lactamase-producing pathogens. Clin Microbiol Rev. 2020;33. doi:10.1128/CMR.00047-19
  • Clinical and Laboratory Standards Institute. M100 Performance Standards for Antimicrobial Susceptibility Testing. 29th ed. Wayne, PA: CLSI; 2019.
  • The European Committee on Antimicrobial Susceptibility Testing. Rapid AST directly from blood culture bottles. Version 10.0, 2020; Available from: http://www.eucast.org. Accessed March 25, 2022.
  • Clinical and Laboratory Standards Institute. M100 Performance Standards for Antimicrobial Susceptibility Testing. 31st ed. Wayne, PA: CLSI; 2021.
  • Sze DTT, Lau CCY, Chan TM, Ma ESK, Tang BSF. Comparison of novel rapid diagnostic of blood culture identification and antimicrobial susceptibility testing by accelerate pheno system and biofire filmarray blood culture identification and biofire filmarray blood culture identification 2 panels. BMC Microbiol. 2021;21(1):350. doi:10.1186/s12866-021-02403-y
  • Keshta AS, Elamin N, Hasan MR, et al. Evaluation of rapid immunochromatographic tests for the direct detection of extended spectrum beta-lactamases and carbapenemases in Enterobacterales isolated from positive blood cultures. Microbiol Spectr. 2021;9(3):e0078521. doi:10.1128/Spectrum.00785-21
  • Bizzini A, Greub G. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry, a revolution in clinical microbial identification. Clin Microbiol Infect. 2010;16:1614–1619. doi:10.1111/j.1469-0691.2010.03311.x
  • Tanner H, Evans JT, Gossain S, Hussain A. Evaluation of three sample preparation methods for the direct identification of bacteria in positive blood cultures by MALDI-TOF. BMC Res Notes. 2017;10:48. doi:10.1186/s13104-016-2366-y
  • Tian Y, Zheng B, Wang B, Lin Y, Li M. Rapid identification and multiple susceptibility testing of pathogens from positive-culture sterile body fluids by a combined MALDI-TOF mass spectrometry and Vitek susceptibility system. Front Microbiol. 2016;7:523. doi:10.3389/fmicb.2016.00523
  • Pan HW, Li W, Li RG, Li Y, Zhang Y, Sun EH. Simple sample preparation method for direct microbial identification and susceptibility testing from positive blood cultures. Front Microbiol. 2018;9:481. doi:10.3389/fmicb.2018.00481
  • Wu S, Xu J, Qiu C, Xu L, Chen Q, Wang X. Direct antimicrobial susceptibility tests of bacteria and yeasts from positive blood cultures by using serum separator gel tubes and MALDI-TOF MS. J Microbiol Methods. 2019;157:16–20. doi:10.1016/j.mimet.2018.12.011
  • Gu YF, Li Y, Zhang XL, Yu LM, Huang BH, Sun CM. A new method aimed to quickly identify pathogen and drug susceptibility test based on matrix-assisted laser desorption/ionization time of flight mass spectrometry combined with flow cytometry. Surg Infect (Larchmt). 2019;20:219–224. doi:10.1089/sur.2018.145
  • Kavipriya D, Prakash SS, Dhandapani S, Rajshekar D, Sastry AS. Evaluation of the performance of direct susceptibility test by VITEK-2 from positively flagged blood culture broth for Gram-negative bacilli. J Lab Physicians. 2021;13(4):374–379. doi:10.1055/s-0041-1732489
  • Gabriele B, Iannaccone M, Boattini M, Cavallo R, Costa C. Assessment of rapid direct E-test on positive blood culture for same-day antimicrobial susceptibility. Braz J Microbiol. 2019;50:953–959. doi:10.1007/s42770-019-00139-6
  • De Gheldre Y, Avesani V, Berhin C, Delmée M, Glupczynski Y. Evaluation of oxoid combination discs for detection of extended-spectrum beta-lactamases. J Antimicrob Chemother. 2003;52:591–597. doi:10.1093/jac/dkg415