Multiplex Digital PCR-Based Development and discussion of the Detection of Genetic Association Between Staphylococcus aureus and mecA

References

• Sanchini A. Recent Developments in Phenotypic and Molecular Diagnostic Methods for Antimicrobial Resistance Detection in Staphylococcus aureus: a Narrative Review. Diagnostics. 2022;12(1):208. doi:10.3390/diagnostics12010208
   Web of Science ®Google Scholar
• Zhao LL, Huang XC, Zhang T, et al. A point-of-care test device for MRSA rapid detection. J Pharmaceutical Biomedical Analysis. 2022;209.
   PubMed Web of Science ®Google Scholar
• Brondo J, Morneau K, Hopkins T, Yang LD, Cadena-Zuluaga J, Walter E. Correlation Between Patients With Methicillin-Resistant Staphylococcus aureus (MRSA) Nares Colonization and MRSA Diabetic Foot Infections. Int J Lower Extremity Wounds. 2022;21(4):502–505. doi:10.1177/1534734620963570
   PubMed Web of Science ®Google Scholar
• Palavecino EL. Clinical, Epidemiologic, and Laboratory Aspects of Methicillin-Resistant Staphylococcus aureus Infections. Methods Mol Biol. 2020;2069:1–28.
   PubMedGoogle Scholar
• Barcudi D, Sosa EJ, Lamberghini R, et al. MRSA dynamic circulation between the community and the hospital setting: new insights from a cohort study. J Infect. 2020;80(1):24–37. doi:10.1016/j.jinf.2019.10.001
   PubMed Web of Science ®Google Scholar
• Lee AS, de Lencastre H, Garau J, et al. Methicillin-resistant Staphylococcus aureus. Nature Reviews Disease Primers. 2018;4(1):18033. doi:10.1038/nrdp.2018.33
   PubMed Web of Science ®Google Scholar
• Lakhundi S, Zhang K. Methicillin-Resistant Staphylococcus aureus: molecular Characterization, Evolution, and Epidemiology. Clin Microbiol Rev. 2018;31(4). doi:10.1128/CMR.00020-18
   PubMed Web of Science ®Google Scholar
• Maree M, Thi Nguyen LT, Ohniwa RL, Higashide M, Msadek T, Morikawa K. Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. Nat Commun. 2022;13(1):2477. doi:10.1038/s41467-022-29877-2
   PubMed Web of Science ®Google Scholar
• Humphries R, Bobenchik AM, Hindler JA, Schuetz AN. Overview of Changes to the Clinical and Laboratory Standards Institute Performance Standards for Antimicrobial Susceptibility Testing, M100. J Clin Microbiol. 2021;59(12):657
   Web of Science ®Google Scholar
• Duan R, Wang P. Rapid and Simple Approaches for Diagnosis of Staphylococcus aureus in Bloodstream Infections. Pol J Microbiol. 2022;71(4):481–489. doi:10.33073/pjm-2022-050
   PubMed Web of Science ®Google Scholar
• Bridwell MR, Bajaj S, Gress TW, Hambuchen MD, Clay TB. Impact of MRSA polymerase chain reaction (PCR) wound swabs on antibiotic de-escalation in skin and soft tissue infections. Diagn Microbiol Infect Dis. 2022;103(3):115722. doi:10.1016/j.diagmicrobio.2022.115722
   PubMed Web of Science ®Google Scholar
• Nnadozie CF, Odume ON. Freshwater environments as reservoirs of antibiotic resistant bacteria and their role in the dissemination of antibiotic resistance genes. Environ Pollut. 2019;254(Pt B):113067. doi:10.1016/j.envpol.2019.113067
   PubMedGoogle Scholar
• Kolman S, Arielly H, Paitan Y. Evaluation of single and double-locus real-time PCR assays for methicillin-resistant Staphylococcus aureus (MRSA) surveillance. BMC Res Notes. 2010;3(1):110. doi:10.1186/1756-0500-3-110
   PubMedGoogle Scholar
• Bishr AS, Abdelaziz SM, Yahia IS, Yassien MA, Hassouna NA, Aboshanab KM. Association of Macrolide Resistance Genotypes and Synergistic Antibiotic Combinations for Combating Macrolide-Resistant MRSA Recovered from Hospitalized Patients. Biology. 2021;10(7). doi:10.3390/biology10070624
   PubMedGoogle Scholar
• Kojabad AA, Farzanehpour M, Galeh HEG, et al. Droplet digital PCR of viral ‎DNA/RNA, current progress, challenges, and future perspectives. J Med Virol. 2021;93(7):4182–4197. doi:10.1002/jmv.26846
   PubMed Web of Science ®Google Scholar
• de la Cruz Barron M, Kneis D, Elena AX, Bagra K, Berendonk TU, Klümper U. Quantification of the mobility potential of antibiotic resistance genes through multiplexed ddPCR linkage analysis. FEMS Microbiol Ecol. 2023;99(4). doi:10.1093/femsec/fiad031
   PubMed Web of Science ®Google Scholar
• Suo T, Liu X, Feng J, et al. ddPCR: a more accurate tool for SARS-CoV-2 detection in low viral load specimens. Emerg Microbes Infect. 2020;9(1):1259–1268. doi:10.1080/22221751.2020.1772678
   PubMed Web of Science ®Google Scholar
• Luo J, Li J, Yang H, Yu J, Wei H. Accurate Detection of Methicillin-Resistant Staphylococcus aureus in Mixtures by Use of Single-Bacterium Duplex Droplet Digital PCR. J Clin Microbiol. 2017;55(10):2946–2955. doi:10.1128/JCM.00716-17
   PubMed Web of Science ®Google Scholar
• Cruciani M, Sandini S, Etna MP, et al. Differential Responses of Human Dendritic Cells to Live or Inactivated Staphylococcus aureus: impact on Cytokine Production and T Helper Expansion. Front Immunol. 2019;10:2622. doi:10.3389/fimmu.2019.02622
   PubMed Web of Science ®Google Scholar
• Kelley K, Cosman A, Belgrader P, Chapman B, Sullivan DC. Detection of methicillin-resistant Staphylococcus aureus by a duplex droplet digital PCR assay. J Clin Microbiol. 2013;51(7):2033–2039. doi:10.1128/JCM.00196-13
   PubMed Web of Science ®Google Scholar
• Chen Y, Shi Y, Zhu W, et al. Combining CRISPR-Cas12a-Based Technology and Metagenomics Next Generation Sequencing: a New Paradigm for Rapid and Full-Scale Detection of Microbes in Infectious Diabetic Foot Samples. Front Microbiol. 2021;12:742040. doi:10.3389/fmicb.2021.742040
   PubMedGoogle Scholar
• Pannewick B, Baier C, Schwab F, Vonberg RP. Infection control measures in nosocomial MRSA outbreaks-Results of a systematic analysis. PLoS One. 2021;16(4):e0249837. doi:10.1371/journal.pone.0249837
   PubMed Web of Science ®Google Scholar
• Brown J, Paladino JA. Impact of Rapid Methicillin-Resistant Staphylococcus aureus Polymerase Chain Reaction Testing on Mortality and Cost Effectiveness in Hospitalized Patients with Bacteraemia. PharmacoEconomics. 2010;28(7):567–575. doi:10.2165/11533020-000000000-00000
   PubMed Web of Science ®Google Scholar
• Hiramatsu K, Hanaki H, Ino T, Yabuta K, Oguri T, Tenover FC. Methicillin-resistant Staphylococcus aureus clinical strain with reduced vancomycin susceptibility. J Antimicrob Chemother. 1997;40(1):135–136. doi:10.1093/jac/40.1.135
   PubMed Web of Science ®Google Scholar
• Bhowmik D, Das BJ, Pandey P, Chetri S, Chanda DD, Bhattacharjee A. An array of multiplex PCR assays for detection of staphylococcal chromosomal cassette mec (SCCmec) types among staphylococcal isolates. J Microbiol Methods. 2019;166:105733. doi:10.1016/j.mimet.2019.105733
   PubMed Web of Science ®Google Scholar
• Pereira EM, Schuenck RP, Malvar KL, et al. Staphylococcus aureus, Staphylococcus epidermidis and Staphylococcus haemolyticus: methicillin-resistant isolates are detected directly in blood cultures by multiplex PCR. Microbiol Res. 2010;165(3):243–249. doi:10.1016/j.micres.2009.03.003
   PubMedGoogle Scholar
• Tsai Y-H, Chen P-H, P-A Y, Chen C-L, Kuo LT, Huang K-C. A multiplex PCR assay for detection of Vibrio vulnificus, Aeromonas hydrophila, methicillin-resistant Staphylococcus aureus, Streptococcus pyogenes, and Streptococcus agalactiae from the isolates of patients with necrotizing fasciitis. Inter J Infect Dis. 2019;81:73–80. doi:10.1016/j.ijid.2019.01.037
   PubMed Web of Science ®Google Scholar
• Wang HY, Kim S, Kim J, Park SD, Uh Y, Lee H. Multiplex real-time PCR assay for rapid detection of methicillin-resistant staphylococci directly from positive blood cultures. J Clin Microbiol. 2014;52(6):1911–1920. doi:10.1128/JCM.00389-14
   PubMed Web of Science ®Google Scholar
• Huggett JF, Foy CA, Benes V, et al. The digital MIQE guidelines: minimum Information for Publication of Quantitative Digital PCR Experiments. Clin Chem. 2013;59(6):892–902. doi:10.1373/clinchem.2013.206375
   PubMed Web of Science ®Google Scholar
• Palavecino EL. Rapid Methods for Detection of MRSA in Clinical Specimens. Methods Mol Biol. 2020;2069:29–45.
   PubMedGoogle Scholar
• Trouillet-Assant S, Rasigade JP, Lustig S, et al. Ward-specific rates of nasal cocolonization with methicillin-susceptible and -resistant Staphylococcus spp. and potential impact on molecular methicillin-resistant Staphylococcus aureus screening tests. J Clin Microbiol. 2013;51(7):2418–2420. doi:10.1128/JCM.00491-13
   PubMed Web of Science ®Google Scholar
• Lipsky BA, Berendt AR, Cornia PB, et al. 2012 Infectious Diseases Society of America clinical practice guideline for the diagnosis and treatment of diabetic foot infections. Clin Infect Dis. 2012;54(12):e132–173. doi:10.1093/cid/cis346
   PubMed Web of Science ®Google Scholar