Formulation and characterization of chlorhexidine HCl nanoemulsion as a promising antibacterial root canal irrigant: in-vitro and ex-vivo studies

References

• Mandal A, Bera A. Surfactant stabilized nanoemulsion: characterization and application in enhanced oil recovery. World Acad Sci Eng Technol. 2012;67:21–26.
   Google Scholar
• Patrycja S, Halina S. Water solubilization using nonionic surfactants from renewable sources in microemulsion systems. J Surfactants Deterg. 2012;15(4):485–494. doi:10.1007/s11743-011-1323-y22707876
   PubMedGoogle Scholar
• Mohan S, Gurtu A, Singhal A, Mehrotra A. Nanotechnology: its implications in conservative dentistry and endodontics. J Dent Sci Or Rehab. 2013;6:9–14.
   Google Scholar
• Rowe RC, Sheskey PJ, Owen SC, editors. Handbook of Pharmaceutical Excipients. London: Pharmaceutical press; 2009:162–165.
   Google Scholar
• Senior N. Some observations on the formulation and properties of chlorhexidine. J Soc Cosmet Chem. 1973;24(4):259–278.
   Google Scholar
• Venghat S, Hegde M, Shetty C. Irrigants used in endodontics. Int J Curr Microbiol App Sci. 2014;3(3):126–132.
   Google Scholar
• Torabinejad M, Handysides R, Khademi A, Bakland LK. Clinical implications of the smear layer in endodontics: a review. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;94:658–666. doi:10.1067/moe.2002.12896212464887
   PubMed Web of Science ®Google Scholar
• Murad CF, Sassone LM, Souza MC, et al. Antimicrobial activity of sodium hypochlorite, chlorhexidine and MTAD® against Enterococcus faecalis biofilm on human dentin matrix in vitro. RSBO Revista Sul-Brasileira de Odontologia. 2012;9(2):143–150.
   Google Scholar
• Plotino G, Cortese T, Grande NM, et al. New technologies to improve root canal disinfection. Braz Dent J. 2016;27(1):3–8. doi:10.1590/0103-644020160072627007337
   PubMedGoogle Scholar
• Gu LS, Kim JR, Ling J, et al. Review of contemporary irrigant agitation techniques and devices. J Endod. 2009;35(6):791–804. doi:10.1016/j.joen.2009.03.01019482174
   PubMed Web of Science ®Google Scholar
• Roni MA, Jalil RU. Comparative study of ibuprofen solubility in synthetic and natural lipid vehicles. Dhaka Univ J Pharma Sci. 2011;10(1):65–66. doi:10.3329/dujps.v10i1.10018
   Google Scholar
• Rushikesh J, Vipul M, Ravindra G, et al. Development and validation of UV-visible spectophotometic method for estimation of selected antiseptic drug in bulk and pharmaceutical dosage form. World J Pharm Pharm Sci. 2016;5(9):1197–1205.
   Google Scholar
• Nornoo AO, Zheng H, Lopes LB, et al. Oral microemulsions of paclitaxel: in situ and pharmacokinetic studies. Eur J Pharm Biopharm. 2009;71(2):310–317. doi:10.1016/j.ejpb.2008.08.01518793723
   PubMed Web of Science ®Google Scholar
• Patel J, Kevin G, Patel A, Raval M, Sheth N. Design and development of a self-nanoemulsifying drug delivery system for telmisartan for oral drug delivery. Int J Pharm Investig. 2011;1(2):112–118. doi:10.4103/2230-973X.82431
   PubMedGoogle Scholar
• Drais HK, Hussein AA. Formulation and characterization of carvedilol nanoemulsion oral liquid dosage form. Int J Pharm Pharm Sci. 2015;7(12):209–216.
   Google Scholar
• Thakur A, Walia MK, Kumar SL. Nanoemulsion in enhancement of bioavailability of poorly soluble drugs: a review. Pharmacophore. 2013;4(1):15–25.
   Google Scholar
• Rajinikanth PS, Suyu Y, Garg S. Development and in-vitro characterization of self-nanoemulsifying drug delivery systems of valsartan. World Acad Sci Eng Technol. 2012;72:1418–1423.
   Google Scholar
• Harika K, Debnath S, Babu MN. Formulation and evaluation of nanoemulsion of amphotericin B. IJNTPS. 2015;4(5):114–122.
   Google Scholar
• Zhang P, Liu Y, Feng N, Xu J. Preparation and evaluation of self microemulsifying drug delivery system of oridonin. Int J Pharm. 2008;355(1–2):269–276. doi:10.1016/j.ijpharm.2007.12.02618242895
   PubMed Web of Science ®Google Scholar
• Agubata CO, Nzekwe IT, Obitte NC, et al. Effect of oil, surfactant and co-surfactant concentrations on the phase behaviour, physicochemical properties and drug release from self-emulsifying drug delivery systems. J Drug Discov Develop and Deliv. 2014;1(1):1–7.
   Google Scholar
• Patel HC, Parmar G, Seth AK, et al. Formulation and evaluation of O/W nanoemulsion of ketoconazole. Int J Pharma Sci. 2013;4(4):338–351.
   Google Scholar
• Singh G, Pai RS. Optimized self-nanoemulsifying drug delivery system of atazanavir with enhanced oral bioavailability: in vitro/in vivo characterization. Expert Opin Drug Deliv. 2014;11(7):1023–1032. doi:10.1517/17425247.2014.91356624820316
   PubMed Web of Science ®Google Scholar
• Xhevdet A, Stubljar D, Kriznar I, et al. The disinfecting efficacy of root canals with laser photodynamic therapy. J Lasers Med Sci. 2014;5(1):19.25606335
   PubMedGoogle Scholar
• Berber VB, Gomes BP, Sena NT, et al. Efficacy of various concentrations of NaOCl and instrumentation techniques in reducing Enterococcus faecalis within root canals and dentinal tubules. Int Endod J. 2006;39(1):10–17. doi:10.1111/j.1365-2591.2005.01038.x16409323
   PubMed Web of Science ®Google Scholar
• Palazzi F, Blasi A, Mohammadi Z, et al. Penetration of sodium hypochlorite modified with surfactants into root canal dentin. Braz Dent J. 2016;27(2):208–216. doi:10.1590/0103-644020160065027058386
   PubMedGoogle Scholar
• Polenik P, Netolicky J Deposition of fluorescent magnetic nanoparticles into dentinal tubules. International Conference on Nanotechnology: Fundamentals and Applications, Prague, Czech 2014;147.
   Google Scholar
• Bumb SS, Bhaskar DJ, Agali CR, et al. Assessment of photodynamic therapy (PDT) in disinfection of deeper dentinal tubules in a root canal system: an in vitro study. Jcdr. 2014;8(11):ZC67. doi:10.7860/JCDR/2014/6788.395625584321
   PubMedGoogle Scholar
• Gomes BP, Ferraz CC, Me V, et al. In vitro antimicrobial activity of several concentrations of sodium hypochlorite and chlorhexidine gluconate in the elimination of Enterococcus faecalis. Int Endod J. 2001;34(6):424–428.11556507
   PubMed Web of Science ®Google Scholar
• Siqueira JF, Machado AG, Silveira RM, et al. Evaluation of the effectiveness of sodium hypochlorite used with three irrigation methods in the elimination of Enterococcus faecalis from the root canal, in vitro. Int Endod J. 1997;30(4):279–282.9477814
   PubMed Web of Science ®Google Scholar
• Agrawal V, Rao MR, Dhingra K, Gopal VR, Mohapatra A, Mohapatra A. An in vitro comparison of antimicrobial effcacy of three root canal irrigants-BioPure MTAD, 2% chlorhexidine gluconate and 5.25% sodium hypochlorite as a final rinse against E. faecalis. J Contemp Dent Pract. 2013;14(5):842–847.24685785
   PubMedGoogle Scholar
• Saber SE, El-Hady SA. Development of an intracanal mature Enterococcus faecalis biofilm and its susceptibility to some antimicrobial intracanal medications; an in vitro study. Eur J Dent. 2012;6(1):43.22229006
   PubMedGoogle Scholar
• Mithra NH, Krishna RS, Shishir S, Veenna SA. Comparative evaluation of bactericidal effects on Enterococcus faecalis using diode laser irradiation, sodium hypochlorite and chlorhexidine gluconate irrigation”-an in vitro study. Oral Health Dent Manag. 2013;12(3):145–150.24352305
   PubMedGoogle Scholar
• Castelo-Baz P, Bahillo J, Seoane-Prado R, Gude F, DeMoor R. Combined sodium hypochlorite and 940 nm diode laser treatment against mature E. faecalis biofilms in-vitro. J Lasers Med Sci. 2012;3(3):116.
   Google Scholar
• Bago I, Plečko V, GabrićPandurić D, et al. Antimicrobial efficacy of a high power diode laser, photo activated disinfection, conventional and sonic activated irrigation during root canal treatment. Int Endod J. 2013;46(4):339–347. doi:10.1111/j.1365-2591.2012.02120.x22970886
   PubMedGoogle Scholar
• Francescutti Murad C, Moura Sassone L, Souza MC, et al. Antimicrobial activity of sodium hypochlorite, chlorhexidine and MTAD against Enterococcus faecalis biofilm on human dentin matrix in vitro. RSBO Revista Sul-Brasileira de Odontologia. 2012;9(2):143–50.
   Google Scholar
• Frough-Reyhani M, Ghasemi N, Soroush-Barhaghi M, et al. Antimicrobial efficacy of different concentration of sodium hypochlorite on the biofilm of Enterococcus faecalis at different stages of development. J Clin Exp Dent. 2016;8(5):e480.27957257
   PubMedGoogle Scholar
• Qazi JI, Asif HI, Shahid R. Economical method for estimation of bacterial viable count. Pakistan J Zool. 2008;40(4):289–294.
   Web of Science ®Google Scholar
• Moghadas L, Shahmoradi M, Narimani T. Antimicrobial activity of a new nanobased endodontic irrigation solution: in vitro study. Dent Hypotheses. 2012;3(4):142. doi:10.4103/2155-8213.106838
   Google Scholar
• Benita S, editor. Microencapsulation: Methods and Industrial Applications. Boca Raton, CRC Press; 2005.
   Google Scholar
• Mason TG, Wilking JN, Meleson K, et al. Nanoemulsions: formation, structure and physical properties. J Phys. 2006;18:R635–R666.
   Google Scholar
• Costa P, Lobo JM. Modeling and comparison of dissolution profiles. Eur J Pharm Sci. 2001;13(2):123–133.11297896
   PubMed Web of Science ®Google Scholar
• Sarpal K, Pawar YB, Bansal AK. Self-emulsifying drug delivery systems: a strategy to improve oral bioavailability. Crips. 2010;11(3):42–49.
   Google Scholar
• Narayanan LL, Vaishnavi C. Endodontic microbiology. J Conserv Dent. 2010;13(4):233. doi:10.4103/0972-0707.7338621217951
   PubMedGoogle Scholar
• Zehnder M. Root canal irrigants. J Endod. 2006;32(5):389–398. doi:10.1016/j.joen.2005.09.01416631834
   PubMed Web of Science ®Google Scholar
• Mohammadi Z, Soltani MK, Shalavi S. An update on the management of endodontic biofilms using root canal irrigants and medicaments. Iran Endod J. 2014;9(2):89.24688576
   PubMedGoogle Scholar
• Portenier I, Waltimo TM, Haapasalo M. Enterococcus faecalis– the root canal survivor and ‘star’in post treatment disease. Endod Topics. 2003;6(1):135–159. doi:10.1111/j.1601-1546.2003.00040.x
   Google Scholar
• Marion J, FranzoniarruDa ME, de MoraiS CA. Chlorhexidine and its applications in Endodontics: a literature review. Dent Press Endod. 2013;3:36–54.
   Google Scholar
• Kamath P, Kundabala M, Shenoy S, et al. An evaluation of horizontal depth of penetration of various irrigants into the dentinal tubules when used alone and in combination with diode laser: an in vitro study. J Interdisc Dent. 2014;4(3):130. doi:10.4103/2229-5194.147331
   Google Scholar
• George S, Kishen A, Song P. The role of environmental changes on monospecies biofilm formation on root canal wall by Enterococcus faecalis. J Endod. 2005;31(12):867–872.16306820
   PubMed Web of Science ®Google Scholar
• Menezes MM, Valera MC, Jorge AO, et al. In vitro evaluation of the effectiveness of irrigants and intracanal medicaments on microorganisms within root canals. Int Endod J. 2004;37(5):311–319. doi:10.1111/j.0143-2885.2004.0079915086752
   PubMedGoogle Scholar
• Ferraz CC, de Almeida Gomes BP, Zaia AA, et al. In vitro assessment of the antimicrobial action and the mechanical ability of chlorhexidine gel as an endodontic irrigant. J Endod. 2001;27(7):452–455.11503994
   PubMed Web of Science ®Google Scholar
• Ahangari Z, Samiee M, Yolmeh MA, Eslami G. Antimicrobial activity of three root canal irrigants on enterococcus faecalis: an in vitro study. Iran Endod J. 2008;3(2):33.24171016
   PubMedGoogle Scholar
• Moritz A, Gutknecht N, Goharkhay K, Schoop U, Wernisch J, Sperr W. In vitro irradiation of infected root canals with a diode laser: results of microbiologic, infrared spectrometric, and stain penetration examinations. Quint Int. 1997;28(3):205–209.
   PubMedGoogle Scholar
• Nusstein JM. Endodontic sonic and ultrasonic irrigant activation. Clin Dent Revi. 2018;2(1):22. doi:10.1007/s41894-018-0037-1
   Google Scholar