Alginate Beads as a Promising Tool for Successful Production of Viable and Pluripotent Human-Induced Pluripotent Stem Cells in a 3D Culture System

References

• Lewitzky M, Yamanaka S. Reprogramming somatic cells towards pluripotency by defined factors. Curr Opin Biotechnol. 2007;18:467–473. doi:10.1016/j.copbio.2007.09.007
   PubMed Web of Science ®Google Scholar
• Lei Y, Schaffer DV. A fully defined and scalable 3D culture system for human pluripotent stem cell expansion and differentiation. Proc Natl Acad Sci USA. 2013;110:E5039–E5048. doi:10.1073/pnas.1309408110
   PubMed Web of Science ®Google Scholar
• Wu X, Su J, Wei J, Jiang N, Ge X. Recent advances in three-dimensional stem cell culture systems and applications. Stem Cells Int. 2021;2021:1–13. doi:10.1155/2021/9477332
   Web of Science ®Google Scholar
• Chaicharoenaudomrung N, Kunhorm P, Noisa P. Three-dimensional cell culture systems as an in vitro platform for cancer and stem cell modeling. World J Stem Cells. 2019;11(12):1065–1083. doi:10.4252/wjsc.v11.i12.1065
   PubMed Web of Science ®Google Scholar
• Bluhmki T, Traub S, Müller A-K, et al. Functional human iPSC-derived alveolar-like cells cultured in a miniaturized 96-Transwell air–liquid interface model. Sci Rep. 2021;11(1). doi:10.1038/s41598-021-96565-4
   PubMed Web of Science ®Google Scholar
• Kinney MA, Sargent CY, McDevitt TC. The multiparametric effects of hydrodynamic environments on stem cell culture. Tissue Eng Part B Rev. 2011;17:249–262. doi:10.1089/ten.teb.2011.0040
   PubMed Web of Science ®Google Scholar
• Chowdhury F, Li Y, Poh YC, Yokohama-Tamaki T, Wang N, Tanaka TS. Soft substrates promote homogeneous self-renewal of embryonic stem cells via downregulating cell-matrix tractions. PLoS One. 2010;5(12):e15655. doi:10.1371/journal.pone.0015655
   PubMed Web of Science ®Google Scholar
• Hwang YS, Cho J, Tay F, et al. The use of murine embryonic stem cells, alginate encapsulation, and rotary microgravity bioreactor in bone tissue engineering. Biomaterials. 2009;30:499–507. doi:10.1016/j.biomaterials.2008.07.028
   PubMed Web of Science ®Google Scholar
• Randle WL, Cha JM, Hwang YS, et al. Integrated 3-dimensional expansion and osteogenic differentiation of murine embryonic stem cells. Tissue Eng. 2007;13:2957–2970. doi:10.1089/ten.2007.0072
   PubMedGoogle Scholar
• Orive G, Hernández RM, Gascón AR, et al. Cell encapsulation: promise and progress. Nat Med. 2003;9:104–107. doi:10.1038/nm0103-104
   PubMed Web of Science ®Google Scholar
• Dean SK, Yulyana Y, Williams G, Sidhu KS, Tuch BE. Differentiation of encapsulated embryonic stem cells after transplantation. Transplantation. 2006;82:1175–1184. doi:10.1097/01.tp.0000239518.23354.64
   PubMed Web of Science ®Google Scholar
• Batorsky A, Liao J, Lund AW, Plopper GE, Stegemann JP. Encapsulation of adult human mesenchymal stem cells within collagen-agarose microenvironments. Biotechnol Bioeng. 2005;92:492–500. doi:10.1002/bit.20614
   PubMed Web of Science ®Google Scholar
• Wang X, Wang W, Ma J, Guo X, Yu X, Ma X. Proliferation and differentiation of mouse embryonic stem cells in APA microcapsule: a model for studying the interaction between stem cells and their niche. Biotechnol Prog. 2006;22(3):791–800. doi:10.1021/bp050386n
   PubMed Web of Science ®Google Scholar
• Dang SM, Gerecht-Nir S, Chen J, Itskovitz-Eldor J, Zandstra PW. Controlled, scalable embryonic stem cell differentiation culture. Stem Cells. 2004;22:275–282. doi:10.1634/stemcells.22-3-275
   PubMed Web of Science ®Google Scholar
• Liu TL, Song K, Song K, et al. Culture of neural stem cells in calcium alginate beads. Biotechnol Prog. 2006;22:1683–1689. doi:10.1002/bp060185z
   PubMed Web of Science ®Google Scholar
• Serra M, Brito C, Correia C, Alves PM. Process engineering of human pluripotent stem cells for clinical application. Trends Biotechnol. 2012;30:350–359. doi:10.1016/j.tibtech.2012.03.003
   PubMed Web of Science ®Google Scholar
• Unger C, Skottman H, Blomberg P, Dilber MS, Hovatta O. Good manufacturing practice and clinical-grade human embryonic stem cell lines. Hum Mol Genet. 2008;17:R48‒R53. doi:10.1093/hmg/ddn079
   PubMed Web of Science ®Google Scholar
• Watanabe K, Ueno M, Kamiya D, et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat Biotechnol. 2007;25:681–686. doi:10.1038/nbt1310
   PubMed Web of Science ®Google Scholar
• Alsobaie S, Alsobaie T, Mantalaris S. Rho-associated protein kinase inhibitor and hypoxia synergistically enhance the self-renewal, survival rate, and proliferation of human stem cells. Stem Cells Cloning. 2022;15(7):43–52. doi:10.2147/SCCAA.S365776
   PubMedGoogle Scholar
• Alsobaie T. Generation of functional mature type II pneumocytes from human induced pluripotent stem cells in 3D dynamic culture [PhD Thesis]. London, UK: Imperial College London; 2019. doi: 10.25560/87943.
   Google Scholar
• Hu Q, Khanna P, Wong BSE, et al. Oxidative stress promotes exit from the stem cell state and spontaneous neuronal differentiation. Oncotarget. 2018;9:4223–4238. doi:10.18632/oncotarget.23786
   PubMedGoogle Scholar
• Mecham RP. Overview of extracellular matrix. Curr Protoc Cell Biol. 2001;10:1. PMID: 18228295. doi:10.1002/0471143030.cb1001s00
   Google Scholar
• Alsobaie S Characterisation of encapsulated embryonic stem cells using silac-based proteomics [Dissertation]. London, UK: Imperial College London; 2016. doi: 10.25560/40561.
   Google Scholar
• Siti-Ismail N, Bishop AE, Polak JM, Mantalaris A. The benefit of human embryonic stem cell encapsulation for prolonged feeder-free maintenance. Biomaterials. 2008;29:3946–3952. doi:10.1016/j.biomaterials.2008.04.027
   PubMed Web of Science ®Google Scholar
• Li YJ, Chung EH, Rodriguez RT, Firpo MT, Healy KE. Hydrogels as artificial matrices for human embryonic stem cell self-renewal. J Biomed Mater Res A. 2006;79:1–5. doi:10.1002/jbm.a.30732
   PubMedGoogle Scholar
• Gerecht S, Burdick JA, Ferreira LS, Townsend SA, Langer R, Vunjak-Novakovic G. Hyaluronic acid hydrogel for controlled self-renewal and differentiation of human embryonic stem cells. Proc Natl Acad Sci USA. 2007;104:11298–11303. doi:10.1073/pnas.0703723104
   PubMed Web of Science ®Google Scholar
• Jha R, Wu Q, Singh M, et al. Simulated microgravity and 3D culture enhance induction, viability, proliferation and differentiation of cardiac progenitors from human pluripotent stem cells. Sci Rep. 2016;6:30956. doi:10.1038/srep30956
   PubMed Web of Science ®Google Scholar
• Grimm D, Wehland M, Pietsch J, et al. Growing tissues in real and simulated microgravity: new methods for tissue engineering. Tissue Eng Part B Rev. 2014;20:555–566. doi:10.1089/ten.teb.2013.0704
   PubMed Web of Science ®Google Scholar
• Yuge L, Kajiume T, Tahara H, et al. Microgravity potentiates stem cell proliferation while sustaining the capability of differentiation. Stem Cells Dev. 2006;15:921–929. doi:10.1089/scd.2006.15.921
   PubMed Web of Science ®Google Scholar
• Han J, Chen L, Luo G, Dai B, Wang X, Dai J. Three-dimensional culture may promote cell reprogramming. Organogenesis. 2013;9:118–120. doi:10.4161/org.24708
   PubMed Web of Science ®Google Scholar
• Claassen DA, Desler MM, Rizzino A. ROCK inhibition enhances the recovery and growth of cryopreserved human embryonic stem cells and human induced pluripotent stem cells. Mol Reprod Dev. 2009;76:722–732. doi:10.1002/mrd.21021
   PubMed Web of Science ®Google Scholar
• Chapman S, McDermott DH, Shen K, Jang MK, McBride AA. The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional. Stem Cell Res Ther. 2014;5:60. doi:10.1186/scrt449
   PubMed Web of Science ®Google Scholar
• Piltti J, Bygdell J, Fernández-Echevarría C, Marcellino D, Lammi MJ. Rho-kinase inhibitor Y-27632 and hypoxia synergistically enhance chondrocytic phenotype and modify S100 protein profiles in human chondrosarcoma cells. Sci Rep. 2017;7:3708. doi:10.1038/s41598-017-03958-5
   PubMed Web of Science ®Google Scholar
• Park JH, Ryu JM, Han HJ. Involvement of caveolin-1 in fibronectin-induced mouse embryonic stem cell proliferation: role of FAK, RhoA, PI3K/Akt, and ERK 1/2 pathways. J Cell Physiol. 2011;226:267–275. doi:10.1002/jcp.22338
   PubMed Web of Science ®Google Scholar
• Yue B. Biology of the extracellular matrix: an overview. J Glaucoma. 2014;23(8 Suppl 1):S20–S23. doi:10.1097/IJG.0000000000000108
   PubMedGoogle Scholar
• Laperle A, Hsiao C, Lampe M, et al. α-5 Laminin synthesized by human pluripotent stem cells promotes self-renewal. Stem Cell Rep. 2015;5:195–206. doi:10.1016/j.stemcr.2015.06.009
   PubMed Web of Science ®Google Scholar
• Domogatskaya A, Rodin S, Tryggvason K. Functional diversity of laminins. Annu Rev Cell Dev Biol. 2012;28:523–553. doi:10.1146/annurev-cellbio-101011-155750
   PubMed Web of Science ®Google Scholar
• Ahmed M, Ffrench-Constant C. Extracellular matrix regulation of stem cell behavior. Curr Stem Cell Rep. 2016;2:197–206. doi:10.1007/s40778-016-0056-2
   PubMed Web of Science ®Google Scholar
• Sevilla CA, Dalecki D, Hocking DC. Regional fibronectin and collagen fibril co-assembly directs cell proliferation and microtissue morphology. PLoS One. 2013;8:e77316. doi:10.1371/journal.pone.0077316
   PubMed Web of Science ®Google Scholar
• García AJ, Vega MD, Boettiger D. Modulation of cell proliferation and differentiation through substrate-dependent changes in fibronectin conformation. Mol Biol Cell. 1999;10:785–798. doi:10.1091/mbc.10.3.785
   PubMed Web of Science ®Google Scholar
• Rodin S, Antonsson L, Niaudet C, et al. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment. Nat Commun. 2014;5:3195. doi:10.1038/ncomms4195
   PubMed Web of Science ®Google Scholar
• Ludwig TE, Levenstein ME, Jones JM, et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol. 2006;24:185–187. doi:10.1038/nbt1177
   PubMed Web of Science ®Google Scholar
• Hosseinkhani H, Hosseinkhani M, Gabrielson NP, Pack DW, Khademhosseini A, Kobayashi H. DNA nanoparticles encapsulated in 3D tissue-engineered scaffolds enhance osteogenic differentiation of mesenchymal stem cells. J Biomed Mater Res A. 2008;85:47–60. doi:10.1002/jbm.a.31327
   PubMed Web of Science ®Google Scholar
• Farias E, Lu M, Li X, Schnapp LM. Integrin alpha8beta1-fibronectin interactions promote cell survival via PI3 kinase pathway. Biochem Biophys Res Commun. 2005;329:305–311. doi:10.1016/j.bbrc.2005.01.125
   PubMed Web of Science ®Google Scholar
• Forristal CE, Wright KL, Hanley NA, Oreffo ROC, Houghton FD. Hypoxia inducible factors regulate pluripotency and proliferation in human embryonic stem cells cultured at reduced oxygen tensions. Reproduction. 2010;139:85–97. doi:10.1530/REP-09-0300
   PubMed Web of Science ®Google Scholar
• Pimton P, Lecht S, Stabler CT, Johannes G, Schulman ES, Lelkes SI. Hypoxia enhances differentiation of mouse embryonic stem cells into definitive endoderm and distal lung cells. Stem Cells Dev. 2015;24:663–676. doi:10.1089/scd.2014.0343
   PubMed Web of Science ®Google Scholar
• Hawkins KE, Sharp TV, McKay TR. The role of hypoxia in stem cell potency and differentiation. Regen Med. 2013;8:771–782. doi:10.2217/rme.13.71
   PubMed Web of Science ®Google Scholar
• Yasmin R, Shah M, Khan SA, Ali R. Gelatin nanoparticles: a potential candidate for medical applications. Nanotechnol Rev. 2017;6(2). doi:10.1515/ntrev-2016-0009
   Web of Science ®Google Scholar
• Rawat S, Sharma Y, Majood M, Mohanty S. 3D Culturing of Stem Cells: An Emerging Technique for Advancing Fundamental Research in Regenerative Medicine. IntechOpen; 2023. doi:10.5772/intechopen.109671
   Google Scholar
• Jensen C, Teng Y. Is it time to start transitioning from 2D to 3D cell culture? Front Molec Bio. 2020;7. doi:10.3389/fmolb.2020.00033
   Web of Science ®Google Scholar