The Current ‘State of Play’ of Regenerative Medicine in Horses: What the Horse Can Tell the Human

References

• Smith RK , BirchHL , GoodmanS , HeinegardD , GoodshipAE . The influence of ageing and exercise on tendon growth and degeneration – hypotheses for the initiation and prevention of strain-induced tendinopathies . Comp. Biochem. Physiol. A Mol. Integr. Physiol.133 , 1039 – 1050 ( 2002 ).
   PubMed Web of Science ®Google Scholar
• Lui PP , MaffulliN , RolfC , SmithRK . What are the validated animal models for tendinopathy?Scand. J. Med. Sci. Sports21 , 3 – 17 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Dudhia J , ScottCM , DraperERet al. Aging enhances a mechanically-induced reduction in tendon strength by an active process involving matrix metalloproteinase activity . Aging Cell6 , 547 – 556 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Lavagnino M , ArnoczkySP , EgerbacherM , GardnerKL , BurnsME . Isolated fibrillar damage in tendons stimulates local collagenase mRNA expression and protein synthesis . J. Biomech.39 , 2355 – 2362 ( 2006 ).
   PubMed Web of Science ®Google Scholar
• Smith RK , GoodshipAE . The effect of early training and the adaptation and conditioning of skeletal tissues . Vet. Clin. North Am. Equine Pract.24 , 37 – 51 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Thorpe CT , StreeterI , PinchbeckGLet al. Aspartic acid racemization and collagen degradation markers reveal an accumulation of damage in tendon collagen that is enhanced with aging . J. Biol. Chem.285 , 15674 – 15681 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Heinemeier KM , SchjerlingP , HeinemeierJ , MagnussonSP , KjaerM . Lack of tissue renewal in human adult Achilles tendon is revealed by nuclear bomb 14C . FASEB J.27 ( 5 ), 2074 – 2049 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Heinemeier KM , BjerrumSS , SchjerlingP , KjaerM . Expression of extracellular matrix components and related growth factors in human tendon and muscle after acute exercise . Scand. J. Med. Sci. Sports23 , e150 – e161 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Mokone GG , GajjarM , SeptemberAVet al. The guanine-thymine dinucleotide repeat polymorphism within the tenascin-C gene is associated with achilles tendon injuries . Am. J. Sports Med.33 , 1016 – 1021 ( 2005 ).
   PubMed Web of Science ®Google Scholar
• Mokone GG , SchwellnusMP , NoakesTD , CollinsM . The COL5A1 gene and Achilles tendon pathology . Scand. J. Med. Sci. Sports16 , 19 – 26 ( 2006 ).
   PubMed Web of Science ®Google Scholar
• Tully LJ , MurphyA , SmithRKWet al. Polymorphisms in TNC and COL5A1 genes are associated with risk of superficial digital flexor tendinopathy in National Hunt Thoroughbred racehorses . Equine Vet. J.46 ( 3 ), 289 – 293 ( 2014 ).
   PubMed Web of Science ®Google Scholar
• Goodship AE , BrownPN , SilverIA , JenkinsD , KirbyM . Use of carbon fibre for tendon repair . Vet. Rec.102 ( 14 ), 322 ( 1978 ).
   PubMed Web of Science ®Google Scholar
• Goodship AE , BrownPN , YeatsJJ , JenkinsDH , SilverIA . An assessment of filamentous carbon fibre for the treatment of tendon injury in the horse . Vet. Rec.106 , 217 – 221 ( 1980 ).
   PubMed Web of Science ®Google Scholar
• Eliashar E , SchrammeMC , SchumacherJ , IkadaY , SmithRK . Use of a bioabsorbable implant for the repair of severed digital flexor tendons in four horses . Vet. Rec.148 , 506 – 509 ( 2001 ).
   PubMed Web of Science ®Google Scholar
• Gibson KT , BurbidgeHM , RobertsonID . The effects of polyester (terylene) fibre implants on normal equine superficial digital flexor tendon . NZ Vet. J.50 , 186 – 194 ( 2002 ).
   PubMed Web of Science ®Google Scholar
• Dowling BA , DartAJ , HodgsonDR , RoseRJ , WalshWR . The effect of recombinant equine growth hormone on the biomechanical properties of healing superficial digital flexor tendons in horses . Vet. Surg.31 , 320 – 324 ( 2002 ).
   PubMed Web of Science ®Google Scholar
• Dahlgren LA , van der MeulenMC , BertramJE , StarrakGS , NixonAJ . Insulin-like growth factor-I improves cellular and molecular aspects of healing in a collagenase-induced model of flexor tendinitis . J. Orthop. Res.20 , 910 – 919 ( 2002 ).
   PubMed Web of Science ®Google Scholar
• Ortved KF , NixonAJ , MohammedHO , FortierLA . Treatment of subchondral cystic lesions of the medial femoral condyle of mature horses with growth factor enhanced chondrocyte grafts: a retrospective study of 49 cases . Equine Vet. J.44 , 606 – 613 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Smith JJ , RossMW , SmithRK . Anabolic effects of acellular bone marrow, platelet rich plasma, and serum on equine suspensory ligament fibroblasts in vitro . Vet. Comp. Orthop. Traumatol.19 , 43 – 47 ( 2006 ).
   PubMed Web of Science ®Google Scholar
• Schnabel LV , MohammedHO , MillerBJet al. Platelet rich plasma (PRP) enhances anabolic gene expression patterns in flexor digitorum superficialis tendons . J. Orthop. Res.25 , 230 – 240 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Schnabel LV , SoneaHO , JacobsonMS , FortierLA . Effects of platelet rich plasma and acellular bone marrow on gene expression patterns and DNA content of equine suspensory ligament explant cultures . Equine Vet. J.40 , 260 – 265 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Castelijns G , CrawfordA , SchafferJet al. Evaluation of a filter-prepared platelet concentrate for the treatment of suspensory branch injuries in horses . Vet. Comp. Orthop. Traumatol.24 , 363 – 369 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Bosch G , van SchieHT , de GrootMWet al. Effects of platelet-rich plasma on the quality of repair of mechanically induced core lesions in equine superficial digital flexor tendons: a placebo-controlled experimental study . J. Orthop. Res.28 , 211 – 217 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Shah M , Foreman , DM , FergusonMWJ . Neutralisation of TGF-β1 and TGF-β2 or exogenous addition of TGF-β3 to cutaneous rat wounds reduces scarring . J. Cell Sci.10 , 985 – 1002 ( 1995 ).
   Google Scholar
• Bosch G , MolemanM , BarneveldA , van WeerenPR , van SchieHT . The effect of platelet-rich plasma on the neovascularization of surgically created equine superficial digital flexor tendon lesions . Scand. J. Med. Sci. Sports21 , 554 – 561 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Waselau M , SutterWW , GenoveseRL , BertoneAL . Intralesional injection of platelet-rich plasma followed by controlled exercise for treatment of midbody suspensory ligament desmitis in Standardbred racehorses . J. Am. Vet. Med. Assoc.232 , 1515 – 1520 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Dominici M , Le BlancK , MuellerIet al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement . Cytotherapy8 , 315 – 317 ( 2006 ).
   PubMed Web of Science ®Google Scholar
• de Mattos Carvalho A , AlvesAL , GolimMAet al. Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue . Vet. Immunol. Immunopathol.132 , 303 – 306 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Ranera B , LyahyaiJ , RomeroAet al. Immunophenotype and gene expression profiles of cell surface markers of mesenchymal stem cells derived from equine bone marrow and adipose tissue . Vet. Immunol. Immunopathol.144 , 147 – 154 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• De Schauwer C , PiepersS , Van de WalleGRet al. In search for cross-reactivity to immunophenotype equine mesenchymal stromal cells by multicolor flow cytometry . Cytometry81 , 312 – 323 ( 2012 ).
   PubMedGoogle Scholar
• Radcliffe CH , FlaminioMJ , FortierLA . Temporal analysis of equine bone marrow aspirate during establishment of putative mesenchymal progenitor cell populations . Stem Cells Dev.19 , 269 – 282 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Clegg PD . Characterisation of mesenchymal stem cells . Presented at : Conference on Regenerative Medicine.Saguaro Lake Ranch , Mesa, AZ, USA , 1 – 5May 2011 .
   Google Scholar
• Barry F , DwyerR , O’BrienT , KavanaghC , DuffyG , MurphyM . Differentiation of mesenchymal stem cells . Presented at : Conference on Regenerative Medicine.Saguaro Lake Ranch , Mesa, AZ, USA , 1 – 5May 2011 .
   Google Scholar
• Li F , LuSJ , HonigGR . Hematopoietic cells from primate embryonic stem cells . Methods Enzymol.418 , 243 – 251 ( 2006 ).
   PubMed Web of Science ®Google Scholar
• Guest DJ , AllenWR . Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts . Stem Cells Dev.16 , 789 – 796 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Guest DJ , SmithMR , AllenWR . Equine embryonic stem-like cells and mesenchymal stromal cells have different survival rates and migration patterns following their injection into damaged superficial digital flexor tendon . Equine Vet. J.42 , 636 – 642 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Watts AE , YeagerAE , KopyovOV , NixonAJ . Fetal derived embryonic-like stem cells improve healing in a large animal flexor tendonitis model . Stem Cell Res. Ther.2 ( 1 ), 4 ( 2011 ).
   PubMedGoogle Scholar
• Hackett CH , FortierLA . Embryonic stem cells and iPS cells: sources and characteristics . Vet. Clin. North Am. Equine Pract.27 , 233 – 242 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Nagy K , SungHK , ZhangPet al. Induced pluripotent stem cell lines derived from equine fibroblasts . Stem Cell Rev.7 , 693 – 702 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Stemcca GM , BressanF , MazieroRet al. 197 induced pluripotent stem cells (iPS) derived from equine umbilical cord cells using lentivirus vector . Reprod. Fertil. Dev.26 ( 1 ), 213 ( 2013 ).
   Google Scholar
• Nishimori M , YakushijiH , MoriMet al. Tumorigenesis in cells derived from induced pluripotent stem cells . Hum. Cell.27 ( 1 ), 29 – 35 ( 2014 ).
   PubMed Web of Science ®Google Scholar
• Vidal MA , KilroyGE , LopezMJ , JohnsonJR , MooreRM , GimbleJM . Characterization of equine adipose tissue-derived stromal cells: adipogenic and osteogenic capacity and comparison with bone marrow-derived mesenchymal stromal cells . Vet. Surg.36 , 613 – 622 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Gimble JM , BunnellBA , ChiuES , GuilakF . Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let’s not get lost in translation . Stem Cells29 , 749 – 754 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Koch TG , HeerkensT , ThomsenPD , BettsDH . Isolation of mesenchymal stem cells from equine umbilical cord blood . BMC Biotechnol.30 ( 7 ), 26 ( 2007 ).
   Google Scholar
• Koch TG , ThomsenPD , BettsDH . Improved isolation protocol for equine cord blood-derived mesenchymal stromal cells . Cytotherapy11 ( 4 ), 443 – 447 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• De Schauwer C , MeyerE , CornilliePet al. Optimization of the isolation, culture, and characterization of equine umbilical cord blood mesenchymal stromal cells . Tissue Eng. Part C Methods.17 ( 11 ), 1061 – 1070 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Cremonesi F , VioliniS , Lange-ConsiglioAet al. Isolation, in vitro culture and characterization of foal umbilical cord stem cells at birth . Vet. Res. Commun. Suppl.1 , S139 – S142 ( 2008 ).
   Google Scholar
• Gittel CB , RibitschJ , BrehmW . Efficiency of adipogenic differentiation methods in mesenchymal stromal cells from diverse sources . Regen. Med.6 , 203 ( 2011 ).
   Web of Science ®Google Scholar
• Burk J , Ribitsch , I , GittelCet al. Growth and differentiation characteristics of equine mesenchymal stromal cells derived from different sources . Vet. J.195 , 98 – 106 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Berg L , KochT , HeerkensTet al. Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood . Vet. Comp. Orthop. Traumatol.22 , 363 – 370 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Lange-Consiglio , A , CorradettiB , BizzaroDet al. Characterization and potential applications of progenitor-like cells isolated from horse amniotic membrane . J. Tiss. Eng. Regen. Med.6 ( 8 ), 622 – 635 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Lange-Consiglio A , CorradettiB , MeucciA , BizzaroD , CremonesiF . Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment . Equine Vet. J.45 ( 6 ), 737 – 744 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Lange-Consiglio A , TassanS , CorradettiBet al. Investigating the efficacy of amnion-derived compared with bone marrow-derived mesenchymal stromal cells in equine tendon and ligament injuries . Cytotherapy15 ( 8 ), 1011 – 1012 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Jones EA , CrawfordA , EnglishAet al. Synovial fluid mesenchymal stem cells in health and early osteoarthritis: detection and functional evaluation at the single-cell level . Arthritis Rheum.58 , 1731 – 1740 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Morito T , MunetaT , HaraKet al. Synovial fluid-derived mesenchymal stem cells increase after intra-articular ligament injury in humans . Rheumatology47 , 1137 – 1143 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Nishimura K , SolchagaLA , CaplanAI , YooJU , GoldbergVM , JohnstoneB . Chondroprogenitor cells of synovial tissue . Arthritis Rheum.42 , 2631 – 2637 ( 1999 ).
   PubMed Web of Science ®Google Scholar
• Endres M , NeumannK , HäuplTet al. Synovial fluid recruits human mesenchymal progenitors from subchondral spongious bone marrow . J. Orthop. Res.25 , 1299 – 1307 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Garvican ER , AlvesAG , SmithRKW , DudhiaJ . Mesenchymal stem cells modulate release of matrix proteins from tendon surfaces in vitro: a potential beneficial therapeutic effect . Regen. Med.9 ( 3 ), 295 – 308 ( 2014 ).
   PubMed Web of Science ®Google Scholar
• Jones EA , EnglishA , HenshawKet al. Enumeration and phenotypic characterization of synovial fluid multipotential mesenchymal progenitor cells in inflammatory and degenerative arthritis . Arthritis Rheum.50 , 817 – 827 ( 2004 ).
   PubMed Web of Science ®Google Scholar
• McCarthy HE , BaraJJ , BrakspearK , SinghraoSK , ArcherCW . The comparison of equine articular cartilage progenitor cells and bone marrow-derived stromal cells as potential cell sources for cartilage repair in the horse . Vet. J.192 , 345 – 351 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Stewart AA , BarrettJG , ByronCRet al. Comparison of equine tendon-, muscle- and bone marrow-derived cells cultured on tendon matrix . Am. J. Vet. Res.70 , 750 – 757 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Alves AG , StewartAA , DudhiaJet al. Cell-based therapies for tendon and ligament injuries . Vet. Clin. North Am. Equine Pract.27 , 315 – 333 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Lange-Consiglio A , RossiD , TassanSet al. Conditioned medium from horse amniotic membrane-derived multipotent progenitor cells: immunomodulatory activity in vitro and first clinical application in tendon and ligament injuries in vivo . Stem Cell Dev.22 ( 22 ), 3015 – 3024 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Koch M , LehnhardtA , HuXet al. Isogeneic MSC application in a rat model of acute renal allograft rejection modulates immune response but does not prolong allograft survival . Transpl. Immunol.29 ( 1–4 ), 43 – 50 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Yoo KH , JangIK , LeeMWet al. Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues . Cell Immunol.259 ( 2 ), 150 – 156 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Guest DJ , OuseyJC , SmithMRW . Defining the expression of marker genes in equine mesenchymal stromal cells . Stem Cells Cloning1 , 1 – 9 ( 2008 ).
   PubMedGoogle Scholar
• Carrade DD , OwensSD , GaluppoLDet al. Clinicopathologic findings following intra-articular injection of autologous and allogeneic placentally derived equine mesenchymal stem cells in horses . Cytotherapy13 ( 4 ), 419 – 430 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Carrade , DD , AffolterVK , OuterbridgeCAet al. Intradermal injections of equine allogeneic umbilical cord-derived mesenchymal stem cells are well tolerated and do not elicit immediate or delayed hypersensitivity reactions . Cytotherapy13 ( 10 ), 1180 – 1192 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Guest DJ , SmithMR , AllenWR . Equine embryonic stem-like cells and mesenchymal stromal cells have different survival rates and migration patterns following their injection into damaged superficial digital flexor tendon . Equine Vet. J.42 ( 7 ), 636 – 642 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Carrade DD , LameMW , KentMSet al. Comparative analysis of the immunomodulatory properties of equine adult-derived mesenchymal stem cells . Cell. Med.4 , 1 – 11 ( 2012 ).
   PubMedGoogle Scholar
• Kang JW , KangKS , KooHCet al. Soluble factors-mediated immunomodulatory effects of canine adipose tissue-derived mesenchymal stem cells . Stem Cells Dev.17 ( 4 ), 681 – 693 ( 2008 ).
   PubMed Web of Science ®Google Scholar
• Fadok VA , BrattonDL , KonowalA , Freed , PW , WestcottJY , HensonPM . Macrophages that have ingested apoptotic cells in vitro inhibit proinflammatory cytokine production through autocrine/paracrine mechanisms involving TGF-beta, PGE2, and PAF . J. Clin. Invest.101 ( 4 ), 890 – 898 ( 1998 ).
   PubMed Web of Science ®Google Scholar
• Schebesch C , KodeljaV , MüllerCet al. Alternatively activated macrophages actively inhibit proliferation of peripheral blood lymphocytes and CD4+ T cells in vitro . Immunology92 ( 4 ), 478 – 486 ( 1997 ).
   PubMed Web of Science ®Google Scholar
• Kim J , HemattiP . Mesenchymal stem cell-educated macrophages: a novel type of alternatively activated macrophages . Exp. Hematol.37 ( 12 ), 1445 – 1453 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Betancourt AM . New cell-based therapy paradigm: induction of bone marrow-derived multipotent mesenchymal stromal cells into pro-inflammatory MSC1 and anti-inflammatory MSC2 phenotypes . Adv. Biochem. Eng. Biotechnol.130 , 163 – 197 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Romieu-Mourez R , FrançoisM , BoivinMN , BouchentoufM , SpanerDE , GalipeauJ . Cytokine modulation of TLR expression and activation in mesenchymal stromal cells leads to a proinflammatory phenotype . J. Immunol.182 , 7963 – 7973 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Betancourt A , BunnellBA . The anti-inflammatory/pro-inflammatory switch of MSCs . Presented at : Conference on Regenerative Medicine.Saguaro Lake Ranch , Mesa, AZ, USA , 1 – 5May 2011 .
   Google Scholar
• Smith RKW , WerlingN , DakinSGet al. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally-occurring tendinopathy . PLoS ONE8 ( 9 ), e75697 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Smith MM , SakuraiG , SmithSMet al. Modulation of aggrecan and ADAMTS expression in ovine tendinopathy induced by altered strain . Arthritis Rheum.58 , 1055 – 1066 ( 2008 ).
   PubMedGoogle Scholar
• Guo Y , SuL , WuJet al. Assessment of the green florescence protein labeling method for tracking implanted mesenchymal stem cells . Cytotechnology64 , 391 – 401 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Kasashima Y , TomitaA , KuwanoA , GoodshipAE , SmithRKW . In vivo tracking of implanted bone marrow-derived mesenchymal stem cells labelled by CM-DiL . Presented at : Conference on Regenerative Medicine.Saguaro Lake Ranch , Mesa, AZ, USA , 1 – 5May 2011 .
   Google Scholar
• Sole A , SprietM , GaluppoLDet al. Scintigraphic evaluation of intra-arterial and intravenous regional limb perfusion of allogeneic bone marrow-derived mesenchymal stem cells in the normal equine distal limb using (99m) Tc-HMPAO . Equine Vet. J.44 , 594 – 599 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Sole A , SprietM , PadgettKAet al. Distribution and persistence of technetium-99 hexamethyl propylene amine oxime-labelled bone marrow-derived mesenchymal stem cells in experimentally induced tendon lesions after intratendinous injection and regional perfusion of the equine distal limb . Equine Vet. J.45 ( 6 ), 726 – 723 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Becerra P , Valdes VazquezMA , DudhiaJet al. Distribution of injected technetium(99m)-labeled mesenchymal stem cells in horses with naturally occurring tendinopathy . J. Orthop. Res.31 , 1096 – 1102 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Trela JM , SprietM , PadgettKAet al. Scintigraphic comparison of intra-arterial injection and distal intra-venous regional limb perfusion for administration of mesenchymal stem cells to the equine foot . Equine Vet. J.45 ( 6 ), 726 – 731 ( 2013 ).
   PubMedGoogle Scholar
• Smith RK , KordaM , BlunnGW , GoodshipAE . Isolation and implantation of autologous equine mesenchymal stem cells from bone marrow into the superficial digital flexor tendon as a potential novel treatment . Equine Vet. J.35 , 99 – 102 ( 2003 ).
   PubMed Web of Science ®Google Scholar
• Schnabel LV , LynchME , van der MeulenMCet al. Mesenchymal stem cells and insulin-like growth factor-I gene-enhanced mesenchymal stem cells improve structural aspects of healing in equine flexor digitorum superficialis tendons . J. Orthop. Res.27 ( 10 ), 1392 – 1398 ( 2009 ).
   PubMed Web of Science ®Google Scholar
• Crovace A , LacitignolaL , RossiG , FranciosoE . Histological and immunohistochemical evaluation of autologous cultured bone marrow mesenchymal stem cells and bone marrow mononucleated cells in collagenase-induced tendinitis of equine superficial digital flexor tendon . Vet. Med. Int.250978 ( 2010 ).
   PubMedGoogle Scholar
• Smith RK , WerlingNJ , DakinSGet al. Beneficial effects of autologous bone marrow-derived mesenchymal stem cells in naturally occurring tendinopathy . PLoS ONE8 ( 9 ), e75697 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Godwin EE , YoungNJ , DudhiaJ , BeamishIC , SmithRK . Implantation of bone marrow-derived mesenchymal stem cells demonstrates improved outcome in horses with overstrain injury of the superficial digital flexor tendon . Equine Vet. J.44 , 25 – 32 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Caniglia CJ , SchrammeMC , SmithRK . The effect of intralesional injection of bone marrow derived mesenchymal stem cells and bone marrow supernatant on collagen fibril size in a surgical model of equine superficial digital flexor tendonitis . Equine Vet. J.44 ( 5 ), 587 – 593 ( 2012 ).
   PubMed Web of Science ®Google Scholar
• Ricco S , RenziS , Del BueMet al. Allogeneic adipose tissue-derived mesenchymal stem cells in combination with platelet rich plasma are safe and effective in the therapy of superficial digital flexor tendonitis in the horse . Int. J. Immunopathol. Pharmacol.26 ( Suppl. 1 ), 61 – 68 ( 2013 ).
   PubMedGoogle Scholar
• Kang JG , ParkSB , SeoMS , KimHS , ChaeJS , KangKS . Characterization and clinical application of mesenchymal stem cells from equine umbilical cord blood . J. Vet. Sci.14 ( 3 ), 367 – 371 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• Fortier LA , PotterHG , RickeyEJet al. Concentrated bone marrow aspirate improves full-thickness cartilage repair compared with microfracture in the equine model . J. Bone. Joint Surg. Am.92 , 1927 – 1937 ( 2010 ).
   PubMed Web of Science ®Google Scholar
• Wilke MM , NydamDV , NixonAJ . Enhanced early chondrogenesis in articular defects following arthroscopic mesenchymal stem cell implantation in an equine model . J. Orthop. Res.25 , 913 – 925 ( 2007 ).
   PubMed Web of Science ®Google Scholar
• Martinello T , BronziniI , PerazziAet al. Effects of in vivo applications of peripheral blood-derived mesenchymal stromal cells (PB-MSCs) and platlet-rich plasma (PRP) on experimentally injured deep digital flexor tendons of sheep . J. Orthop. Res.31 , 306 – 314 ( 2013 ).
   PubMed Web of Science ®Google Scholar
• McIlwraith CW , FrisbieDD , RodkeyWGet al. Evaluation of intra-articular mesenchymal stem cells to augment healing of microfractured chondral defects . Arthroscopy27 , 1552 – 1561 ( 2011 ).
   PubMed Web of Science ®Google Scholar
• Frisbie DD , StewartMC . Cell-based therapies for equine joint disease . Vet. Clin. North Am. Equine Pract.27 , 335 – 349 ( 2011 ).
   PubMed Web of Science ®Google Scholar
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   Google Scholar