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Original Article

Culture of human endometrial cells: a new simple technique to completely separate epithelial glands

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Pages 87-92 | Accepted 07 Sep 1992, Published online: 03 Aug 2009

References

  • Bayard F, Damilano S, Robel P, Baulieu E E. Estradiol and progesterone receptors in human endometrium. J Clin Endocrinol Metab 1978; 46: 635–47
  • Tseng L, Gurpide E. Induction of human endometrial estradiol dehydrogenase by progestins. Endocrinology 1975; 97: 825–33
  • Tseng L, Gurpide E. Estradiol and 20α-dihydroprogestero-ne dehydrogenase activities in human endometrium during the menstrual cycle. Endocrinology 1974; 94: 419–23
  • Bayard F, Louvet J P, Monrozies M, Boulard A, Pontonnier G. Endometrial progesterone concentrations during the menstrual cycle. J Clin Endocrinol Metab 1975; 41: 412–4
  • Tseng L, Gurpide E. Changes in the in vitro metabolism of estradiol by human endometrium during the menstrual cycle. J Obstet Gynecol 1972; 114: 1002–8
  • Chen L, Lindner H R. Mitogenic action of estradiol 17-P on human myometrial and endometrial cells in long-term tissue cultures. J Endocrinol 1973; 59: 87–97
  • Tseng L, Stolee A, Gurpide E. Quantitative studies on the uptake and metabolism of estrogens and progesterone by human endometrium. Endocrinology 1972; 90: 390–404
  • Liu H, Tseng L. Estradiol metabolism in isolated human endometrial epithelial glands and stromal cells. Endocrinology 1979; 104: 1674–81
  • Cooke P S, Uchima F A, Fujii D K, Bern H A. Restoration of normal morphology and estrogen responsiveness in cultured vaginal and uterine epithelia transplanted with stroma. Proc Natl Acad Sci 1986; 83: 2109–13
  • Roberts D K, Walker N J, Lavig L A. Ultrastructural evidence of stromal-epithelial interactions in the human endometrial cycle. Am J Obstet Gynecol 1988; 158: 854–61
  • Cunha G R, Bigsby R M, Cooke P S, Sugimura Y. Stromal-epithelial interactions in adult organs. Cell Diff 1985; 17: 137–48
  • Satyaswaroop P G, Bressler R S, De la Pena M M, Gurpide E. Isolation and culture of human endometrial glands. J Clin Endocrinol Metab 1979; 48: 639–41
  • Varma V A, Melin S A, Adamec T A, et al. Monolayer culture of human endometrium: methods of culture and identification of cell types. J Clin Endocrinol Metab 1982; 18: 911–4
  • Kirk D, King R JB, Heyes J, Peachey L, Hirsch P J, Taylor R WT. Normal human endometrium in cell culture. 1. Separation and characterization of epithelial and stromal components in vitro. J Clin Endocrinol Metab 1978; 14: 651–62
  • Osteen K G, Hill G A, Hargrove J T, Gorstein F. Development of a method to isolate and culture highly purified populations of stromal and epithelial cells from human endometrial biopsy specimens. Fertil Steril 1989; 52: 965–72
  • Trent J M, Davis J R, Payne C M. The establishment and morphologic characterization of finite cell lines from normal human endometrium. Am J Obstet Gynecol 1980; 136: 352–62
  • Centola G M, Cisar M, Knab D R. Establishment and morphologic characterization of normal human endometrium in vitro. Am J Obstet Gynecol 1984; 20: 451–62
  • Sternberg I A. The unlabeled antibody peroxidase antiper-oxidase (PAP) method. Immunocytochemis-try, J Wiley. Wiley-Lis, New York 1979; 104–69
  • Moll R, Franke W W, Schiller D L, Geiger B, Krepler R. The catalog of human cytokeratins: pattern of expression in normal epithelia, tumors and cultured cells. Cell 1982; 31: 11–20
  • Viganó' P, Vercellini P, Di Blasio A M, Colombo A, Candi-Ani G B, Vignali M. Deficient anti-endometrium lymphocyte-mediated cytotoxicity in patients with endometriosis. Fertil Steril 1991; 56: 894–9

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