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Scandinavian Journal of Clinical and Laboratory Investigation Volume 41, 1981 - Issue 6
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Original Article

Application of the law of mass action to design and performance of receptor assays for 1,25(OH)2 vitamin D

J. T. Dabek Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
,
M. Härkönen Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
&
H. Adlercreutz Department of Clinical Chemistry, University of Helsinki, Meilahti Hospital, Helsinki, Finland
Pages 543-550 | Received 19 May 1980, Accepted 10 Jan 1981, Published online: 08 Jul 2009

References

  • Brumbaugh P. F., Haussler D. H., Bressler R., Haussler M. R. Radioreceptor assay for 1α,25-dihydroxy-vitamin D3. Science 1974; 183: 1089
  • Brumbaugh P. F., Haussler D. H., Bursa K. M., Haussler M. R. Filter assay for 1α,25-dihydroxy-vitamin D3. Utilization of the hormones target tissue chromatin receptor. Biochemistry 1974; 13: 4091
  • Dabek J. T. Plasma profile of hydroxylated vitamin D metabolites: methods and results in normals for spring-winter in southern Finland. Ann. clin. Res 1980; 12: 17
  • Dabek J. T., Härkönen M., Adlercreutz H. A sensitive simplified receptor assay for 1,25(OH)2 vitamin D. Scand. J. clin. Lab. Invest 1981; 41: 151
  • Eisman J. A., Hamstra A. J., Kream B. E., De Luca M. F. A sensitive, precise and convenient method for determination of 1,25-dihydroxy vitamin D in human plasma. Arch. Biochem. Biophys 1976; 176: 235
  • Lund B., Lund B., Sørensen O. H. Measurement of circulating 1,25-dihydroxyvitamin D in man. Changes in serum concentrations during treatment with lα-hydroxy-cholecalciferol. Acta endocr. (Kbh) 1979; 91: 338
  • Rodbard D., Munson P. J., De Lean A. Improved curve-fitting, parallelism testing, characterization of sensitivity and specificity, validation and optimization for radioligand assays. Radioimmunoassay and Related Procedures in Medicine. International Atomic Energy Agency, Vienna 1978; Vol. 1.: 469
  • Taylor C. M., Hann J., St. John J., Wallace J. E., Mawer E. B. 1,25-Dihydroxycholecalciferol in human serum and its relationship with other metabolites of vitamin D-3. Clin. chim. Acta 1979; 96: 1
  • Wilson D., Sarfaty G., Clarris B., Douglas M., Crawshaw K. The prediction of standard curves & errors for the assay of estradiol by competitive protein binding. Steroids 1971; 18: 77
  • Yalow R. S., Berson S. A. General principles of radioimmunoassay. Radioisotopes in Medicine: in vitro studies, R. L. Hayes, F. A. Goswitz, B. E.P. Murphy. US Atomic Energy Agency: Symposium, Oak Ridge 1968; 32, Series No. 13

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