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Archives of Andrology
Journal of Reproductive Systems
Volume 17, 1986 - Issue 2
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Original Article

Ultrastructural Changes in the Acrosome of Human Sperm During Freezing and Thawing: A Pilot Trial

E. E. Oettlé Departments of Genesiology and Anatomy, Faculty of Veterinary Science, University of Pretoria, P. O. Box 12580, 0110, Onderstepoort, Republic of South Africa
&
J. T. Soley Departments of Genesiology and Anatomy, Faculty of Veterinary Science, University of Pretoria, P. O. Box 12580, 0110, Onderstepoort, Republic of South Africa
Pages 145-150 | Published online: 09 Jul 2009

References

  • Foote RH. Semen quality from the bull to the freezer: An assessment. Theriogeneology 1975; 3(6)219–234
  • Hackett A J, Macpherson JW. Some staining procedures for spermatozoa. A review. Can Vet J 1965; 6: 55–62
  • Harasymawycz J, Ball L, Seidel GE. Evaluation of bovine spermatozoal morphologic features after staining or fixation. Am J Vet Res 1976; 37: 1053–1057
  • Oettlé EE. Infertility in a Maltese poodle as a result of a sperm midpiece defect. J S Afr Vet Assoc 1985; 56(2)103–106
  • Oettlé EE. Changes in acrosome morphology during cooling and freezing of dog semen. Ann Reprod Sci 1986, in press
  • Pedersen H, Lebech PE. Ultrastructural changes in the human spermatozoon after freezing for artificial insemination. Fertil Steril 1971; 22(2)125–133
  • Polge C. Freezing of spermatozoa. Low Temperature Preservation in Medicine and Biology, MJ Ashwood-Smith, J Farrant. Pitman Medical Ltd., KentU.K. 1980; 45–64
  • Saake R G, White JM (1972) Semen quality tests and their relationship to fertility. In: Natl. Assoc. Anim. Breeders. Proceedings: IVth Tech Conf Artif Insem Reprod. 1972, 22–27
  • Schneider H. The evaluation of cryoprotective media for human semen. Frozen Human Semen—A Workshop upon the Cryobiology, and Its Role, in Artificial Insemination by Donor, D Richardson, D Joyce, M Symonds. Royal College of Obstetrics and Gynaecology, London 1979; 59–68
  • Watson PF. Use of a Giemsa stain to detect changes in acrosomes of frozen ram spermatozoa. Vet Rec 1975; 97: 12–15

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