19
Views
34
CrossRef citations to date
0
Altmetric
Original Article

Expression of Cyp2B1 in Freshly Isolated and Proliferating Cultures of Epithelial Rat Lung Cells

, , , , , & show all
Pages 627-649 | Received 05 May 1995, Accepted 17 Jun 1996, Published online: 02 Jul 2009

References

  • Sorokin SP. The cells of the lung. Morphology of Experimental Respiratory Carcinogenesis, P Nettesheim. AEC Symposium Series, 1970; 21: 3–44
  • Devereux TR, Domin BA, Philpot RM. Xenobiotic metabolism by isolated pulmonary cells. Pharmacol Ther. 1989; 41: 243–256
  • Boyd MR. Evidence for the Clara cell as a site of cytochrome P450-dependent mixed-function oxidase activity in lung. Nature 1977; 269: 713–715
  • Devereux TR, Fouts JR. Isolation of pulmonary cells and use in studies of xenobiotic metabolism. Methods of Enzymology, W Jakoby. Academic, New York 1981; 77: 147–154
  • Devereux TR, Fouts JR. Xenobiotic metabolism by alveolar type II cells isolated from rabbit lung. Biochem Pharmacol. 1981; 30: 1231–1237
  • Philpot RM, Wolf CR. The properties and distribution of the enzymes of pulmonary cytochrome P-450-dependent monoxygenase systems. Rev Biochem Toxicol 1981; 3: 51–76
  • Minchin RF, Boyd MR. Localization of metabolic activtion and deactivation systems in the lung: significance to the pulmonary toxicity of xenobiotics. Annu Rev Pharmacol Toxicol. 1983; 23: 217–238
  • Baron J, Voigt JM. Localization, distribution, and induction of xenobiotic-metabolizing enzymes and aryl hydrocarbon hydroxylase activity within lung. Pharmacol Ther. 1990; 47: 419–445
  • Serabjit-Singh CJ, Wolf CR, Philpot RM. Cytochrome P-450: localization in rabbit lung. Science 1980; 207: 1469–1470
  • Serabjit-Singh CJ, Nishio SJ, Philpot RM, Plopper CG. The distribution of cytochrome P-450 monooxygenase in cells of rabbit lung: an ultrastructural immunocytochemical characterization. Mol Pharmacol. 1988; 33: 279–289
  • Guengerich FP. Purification and characterization of xenobiotic-metabolizing enzymes from lung tissue. Pharmacol Ther. 1990; 45: 299–307
  • De Waziers I, Cugnenc PH, Yang CS, Leroux J-P, Beaune PH. Cytochrome P450 isoenzymes, epoxide hydrolase and glutathione tranferases in rat and human hepatic and extrahepatic tissues. J Pharmacol Exp Ther. 1990; 253: 387–394
  • Kimura S, Kozak CA, Gonzalez FJ. Identification of a novel P450 expressed in rat lung: cDNA cloning and sequence, chromosome mapping, and induction by 3-methylcholanthrene. Bio-chemistry 1989; 28: 3798–3803
  • Bissell DM, Guzelian PS. Microsomal functions and phenotypic change in adult rat hepatocytes in primary monolayer culture. Gene Expression and Carcinogenesis in Cultured Liver, LE Gerchenson, EB Thompson. Academic, New York 1975; 119–136
  • Steward AR, Dannan GA, Guzelian PS, Guengerich FP. Changes in the concentration of seven forms of cytochrome P-450 in primary cultures of adult rat hepatocvtes. Mol Pharmacol. 1985; 27: 125–132
  • Reiners JJ, Jr, Pavone A, Cantu AR, Auerbach C, Malkinson AM. Differential expression of cytochrome P-450 in proliferating and quiescent cultures of murine lung epithelial cells. Biochem Biophys Res Commun. 1992; 183: 193–198
  • Kauffman SL. Cell proliferation in the mammalian lung. Int Rev Exp Pathol. 1980; 22: 131–191
  • Haugen A, Aune I. Culture of rabbit pulmonary Clara cells. Proc Soc Exp Biol Med. 1986; 182: 277–281
  • Richards RJ, Davies N, Atkins J, Oreffo VIC. Isolation, biochemical characterization, and culture of lung type II cells of the rat. Lung. 1987; 165: 143–158
  • Kalina M, Riklis S. Cytochemical approach to the development and behaviour of rat alveolar type II cells in culture. Histochemistry 1988; 88: 175–179
  • Adamson IYR, King GM, Young L. Influence of extracellular matrix and collagen components on alveolar type II cell morphology and function. In Vitro Cell Dev Biol. 1989; 25: 494–502
  • Dobbs LG. Isolation and culture of alveolar type II cells. Am J Physiol. 1990; 258: L134–L147
  • Leslie CC, McCormick-Shannon K, Mason RJ, Shannon JM. Proliferation of rat alveolar epithelial cells in low density primary culture. Am J Respir Cell Mol Biol. 1993; 9: 64–72
  • Kalina M, Riklis S, Blau H. Pulmonary epithelial cell proliferation in primary culture of alveolar type II cells. Exp Lung Res. 1993; 19: 153–175
  • Johansson I, Ekstrøm G, Scholte B, Puzycki D, Jornvall H, Ingelman-Sundberg M. Ethanol-, fasting-, and aceton-inducible cytchromes P450 in rat liver: regulation and characteristics of enzymes belonging to the IIB and HE gene subfamilies. Biochemistry 1988; 27: 1925–1934
  • Lund J, Xordlund L, Gustafsson J. Partial purification of a binding protein for polychlorinated biphenyls from rat lung cytosol: physicochemical and immunochemical characterization. Biochemistrv. 1988; 27: 7895–7901
  • Dahl JE, Becher R, Låg M, Schuller HM, Dybing E. Formation of genotoxic products from N'-nitrosoheptamethyleneimine (NHMI), 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN) by isolated rabbit lung cells. Cell Biol Toxicol. 1990; 6: 399–409
  • Dobbs LG, Gonzalez R, Williams MC. An improved method for isolating type II cells in high yield and purity. Am Rev Respir Dis. 1986; 134: 141–145
  • Mason R f, Walker SR, Shields BA, Henson JE, Williams MC. Identification of rat alveolar type II epithelial cells with a tannic acid and polychrome stain. Am Rev Respir Dis. 1985; 131: 786–788
  • Huitfeldt HS, Brandtzaeg P. Various keratin antibodies produce immunohistochemical staining of human myocardium and myometrium. Histochemistry 1985; 83: 381–389
  • Vindeløv LL, Christensen IJ, Nissen NI. A detergent-trypsin method for the preparation of nuclei for flow cytometric DNA analysis. Cytometry 1983; 3: 323–327
  • Adesnik M, Bar-Nun S, Maschio F, Zunich M, Lippman A, Baird E. Mechanism of induction of cytochrome P-450 by phenobarbital. J Biol Chem. 1981; 256: 10340–10345
  • Boehringer Mannheim GmbH, Biochemica. The DIG System User's Guide for Filter Hybridization. Mannheim, Germany 1993
  • Omiecinski CJ. Tissue-specific expression of rat mRNAs homologous to cytochromes P-450b and P-450e. Nucleic Acids Res. 1986; 14: 1525–1539
  • Scholte BJ, Te Velde B, Groot GSP, De Vries J. Induction of cytochrome P-450 b, e-type isozymes by polychlorinated biphenyls in rat liver: molecular cloning of induced mRNA. Eur J Biochem. 1985; 151: 67–73
  • Cleveland DW, Lopata MA, Macdonald RJ, Cowan NJ, Rutter WJ, Kirschner MW. Number and evolutionary conservation of α- and β-tubulin and cytoplasmic β- and γ-actin genes using specific cloned cDNA probes. Cell. 1980; 20: 95–105
  • Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987; 162: 156–159
  • Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press;, New York 1989
  • Dajani OF, Refsnes M, Guren TK, Horn RS, Thoresen GH, Christoffersen T. Elevated glucose concentrations inhibit DNA synthesis and expression of c-myc in cultured hepatocytes. Biochem Biophys Res Commun. 1994; 202: 1476–1482
  • Kendrick NC, Johansen JJ, Lee PR, Santek DA. Optimization of an HP Scanjet for quantification of protein electrophoresis gels. Anal Biochem. 1994; 219: 297–304
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680–685
  • Mikalsen A, Alexander J, Wallin H, Ingelman-Sundberg M, Andersen RA. Reductive metabolism and protein binding of chromium(VI) by P450 protein enzymes. Carcinogenesis. 1991; 12: 825–831
  • Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979; 76: 4350–4354
  • Guy J, Ramasubbareddy D, Mukherjee AB. Surfactant-producing rabbit pulmonary alveolar type II cells synthesize and secrete an antiinflammatory protein, uteroglobin. Biochem Biophys Res Commun. 1992; 189: 662–669
  • Jones KG, Holland JF, Fouts JR. Benzo(a)pyrene hydroxylase activity in enriched populations of Clara cells and alveolar type II cells from control and β-naphthoflavone-pretreated rats. Cancer Res. 1982; 42: 4658–4663
  • Rabovsky J, Judy DJ, Sapola NA, Pailes WH, McPeek M, Castranova V. Cytochrome P450-dependent alkoxyphenoxazone dealkylase activity in rat alveolar type II cells: effect of pretreatment with β-naphthoflavone. Cell Biochem Func. 1989; 7: 79–89
  • Plopper CG, Hyde DM, Buckpitt AR, et al. Clara cells. The Lung: Scientific Foundations, RG Crystal, JB West. Raven, New York 1991; 215–228
  • Martin J, Dinsdale D, White INH. Characterization of Clara and type II cells isolated from rat lung by fluorescence-activated flow cytometry. Biochem J. 1993; 295: 73–80
  • Palmer KC. Clara cell adenomas of the mouse lung. Interaction with alveolar type 2 cells. Am J Pathol. 1985; 120: 455–163
  • Jones KG, Holland JF, Foureman GL, Bend JR, Fouts JR. Xenobiotic metabolism in Clara cells and alveolar type II cells isolated from lungs of rats treated with β-naphthoflavone. J Pharmacol Exp Ther. 1983; 225: 316–319
  • Marcus CB, Wilson NM, Keith IM, Jefcoate CR, Omiecinski CJ. Selective expression of cytochrome P450 isozymes by 4-n-alkyl-methylenedioxybenzenes in rat lung cells. Arch Biochem Biophys. 1990; 277: 17–25
  • Wortelboer HM, De Kruif CA, Van Iersel AAJ, Falke HE, Noordhoek J, Blaauboer BJ. The isoenzyme pattern of cytochrome P450 in rat hepatocytes in primary culture, comparing different enzyme activities in microsomal incubations and in intact monolayers. Biochem Pharmacol. 1990; 40: 2525–2534
  • Kocarek TA, Schuetz EG, Guzelian PS. Expression of multiple forms of cytochrome P450 mRNAs in primary cultures of rat hepatocytes maintained on matrigel. Mol Pharmacol. 1993; 43: 328–334
  • Marie IJ, Dalet C, Blanchard J-M, et al. Inhibition of cytochrome P-450p (P450 IIIA3) gene expression during liver regeneration from two-thirds hepatectomv in the rat. Biochem Pharmacol. 1988; 37: 3515–3521
  • Liddle C, Murray M, Kartell GC. Effect of liver regeneration on hepatic cytochrome P450 isozymes and serum sex steroides in the male rat. Gastroenterology 1989; 96: 864–872

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.