1
Views
6
CrossRef citations to date
0
Altmetric
Original Article

New Techniques:The Use of Fixed-Frozen Tissue in Routine Ultrastructural Evaluation and Immunogold Labeling

, , &
Pages 351-362 | Accepted 13 Aug 1991, Published online: 10 Jul 2009

References

  • Stirling J. W. Immuno- and affinity probes for electron microscopy: a review of labeling and preparation techniques. J Histochem Cyto-chem. 1990; 38: 147–175
  • Carlemalm E., Garavito R. M., Villiger W. Resin development for electron microscopy and an analysis of embedding at low temperature. J Microsc. 1982; 126: 123–143
  • Roth J. Postembedding labeling on Lowicryl K4M tissue sections: detection and modification of cellular components. Methods Cell Biol. 1989; 31: 513–551
  • Luft J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961; 9: 409–414
  • Loesser K. E., Doane K. J., Wilson F. J., Roisen F. J., Malamed S. Improved immunoelectron micro-scopic method for localizing cytoskeletal proteins in Lowicryl K4M embedded tissues. J Histochem Cytochem. 1986; 34: 1477–1485
  • Scopsi L., Larsson L. I., Bastholm L., Hartvig-Nielsen M. Silver-enhanced colloidal gold probes as markers for scanning electron microscopy. Histochemistry. 1986; 86: 35–41
  • Scopsi L., Pilotti S., Rilke F. Immunocytochemical localization and identification of members of the pancreatic polypeptide (PP)-fold family in human thyroid C cells and medullary carcinomas. Regul Peptides. 1990; 30: 89–104
  • Bendayan M. Double immunocytochemical labeling applying the protein A-gold technique. J Histochem Cytochem. 1982; 30: 81–85
  • Larsson L-I., Scopsi L., Modena D., Racchetti G., Galante Y. M. Immunocytochemical characterization of mouse monoclonal ACTH antibodies with a note on staining conditions and control procedures. Histochemistry. 1989; 91: 115–122
  • McGinley D. M., Posalaky Z., Posalaky I. P. The use of fresh-frozen tissue in diagnostic electron microscopy. Ultrastruct Pathol. 1984; 6: 89–98
  • Iguchi H., Natori S., Kato K., Nawata H., Chretien M. Different processing of chomogranin B into GAWK-immunoreactive fragments in the bovine adrenal medulla and pituitary gland. Life Sci. 1988; 43: 1945–1952
  • Ottersen O. P., Storm-Mathisen J. Glutamate-and GABA-containing neurons in the mouse and rat brain, as demonstrated with a new immunocytochemical technique. J Comp Neurol. 1984; 229: 374–392
  • Madsen O. D., Cohen R. M., Fitch F. W., Rubenstein A. H., Steiner D. F. The production and characterization of monoclonal antibodies specific for human proinsulin using a sensitive microdot assay procedure. Endocrinology. 1983; 113: 2135–2144
  • Scopsi L., Ferrari C., Pilotti S., et al. Immunocytochemical localization and identification of prosomatostatin gene products in medullary carcinoma of human thyroid gland. Hum Pathol. 1990; 21: 820–830
  • Comitti R., Racchetti G., Gnocchi P., Morandi E., Galante Y. M. A monoclonal-based, two-site enzyme immunoassay of human insulin. J Immunol Methods. 1987; 99: 25–37
  • Scopsi L., Arias J., Houen G., Racchetti G., Fos-sati G. L., Galante Y. M. Monoclonal antibodies against calcitonin. Characterization and application in light and electron microscopy im-munocytochemistry. Histochemistry. 1988; 88: 113–125
  • Orskov C., Hoist J. J. Radio-immunoassays for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2). Scand J C/in Lab Invest. 1987; 47: 165–174
  • Larsson L-I., Stengaard-Pedersen K. Enkephalin/endorphin-related peptides in antro-pyloric gastrin cells. J Histochem Cytochem. 1981; 29: 1088–1098
  • Larsson L-L., Rehfeld J. F. Characterization of antral gastrin cells with region-specific anti-sera. J Histochem Cytochem. 1977; 25: 1317–1321
  • Pfahl H., Heilig B., Bethäuser H., Hüfner M., Schmidt-Gayk H., Junker M. Development of immunoradiometric assay for human thyro-globulin using monoclonal antibodies and the biotin/avidin system. J Clin Chem Clin Biochem. 1988; 26: 435–439

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.