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Original Article

Prolonged retention of cross-linked trypsin in calcium alginate microspheres

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Pages 51-61 | Received 16 Aug 1995, Accepted 09 Nov 1995, Published online: 26 Jun 2009

References

  • Bond J. S. Commercially available proteases. Proteolytic Enzymes—A Practical Approach, R. J. Beynon, J. S. Bond. IRL Press, Oxford 1989, Appendix II
  • Broun G. B. Chemically aggregated enzymes. Methods in Enzymology XLIV— Immobilised Enzymes, K. Mosbach, 1976; 263–280
  • Clair N. L.St, Navia M. A. Cross-linked enzyme crystals as robust biocatalysts. Journal American Chemical Socity 1992; 114: 7314–7316
  • Khan S. S., Siddiqi A. M. Studies on chemically aggregated pepsin using glutaraldehyde. Biotechnology and Bioengineeing 1985; XXVII: 415–419
  • Nakagawa T., Izawa K., Yagi S., Shibukawa A., Tanaka H., Tashima T., Imai M. Development of effective cross-linking method for bioactive substance-enzyme immobilization using glutaraldehyde oligomers. Chemical and Pharmaceutical Bulletin 1989; 37(9)2463–2466
  • Tanaka H., Matsumura M., Veliky I. A. Diffusion characteristics of substrates in Ca-Alginate gel beads. Biotechnology and Bioengineering 1983; XXVI: 053–058
  • Tanaka H., K-Urosawa H., Kokufuta E., Veliky I. A. Preparation of immobilized glucoamylase using Ca-Alginate gel coated with partially quarternized poly(ethy1eneimine). Biotechnology and Bioengineering 1984; XXVI: 1393–1394
  • Wong S. S., Wong L. J. C. Chemical crosslinking and the stabilization of proteins and enzymes. Enzyme and Microbiological Technology 1992; 14: 866–874

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