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Hemoglobin
international journal for hemoglobin research
Volume 34, 2010 - Issue 2
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Short Communication

A Rapid Single-Tube Multiplex Polymerase Chain Reaction Assay for the Seven Most Prevalent α-Thalassemia Deletions and αααanti 3.7 α-Globin Gene Triplication

Arjan de Mare Medisch Spectrum Twente Hospital Group, Enschede, The Netherlands
,
Antoinette Heijs-Oude Groeneger Medisch Spectrum Twente Hospital Group, Enschede, The Netherlands
,
Sander Schuurman Medisch Spectrum Twente Hospital Group, Enschede, The Netherlands
,
Frank A.T.J.M. van den Bergh Medisch Spectrum Twente Hospital Group, Enschede, The Netherlands
&
Jennichjen Slomp Medisch Spectrum Twente Hospital Group, Enschede, The NetherlandsCorrespondence[email protected]
Pages 184-190 | Received 18 Aug 2009, Accepted 24 Dec 2009, Published online: 30 Mar 2010

REFERENCES

  • Urbinati F, Madigan C, Malik P. Pathophysiology and therapy for haemoglobinopathies. Part II: thalassaemias. Expert Rev Mol Med. 2006;8(10):1–26.
  • Higgs DR, Weatherall DJ. The α thalassaemias. Cell Mol Life Sci. 2009;66(7):1154–1162.
  • Voon HP, Vadolas J. Controlling α-globin: a review of α-globin expression and its impact on β-thalassemia. Haematologica. 2008;93(12):1868–1876.
  • Trent RJ, Higgs DR, Clegg JB, Weatherall DJ. A new triplicated α-globin gene arrangement in man. Br J Haematol. 1981;49(1):149–152.
  • Goossens M, Dozy AM, Embury SH, Triplicated α-globin loci in humans. Proc Natl Acad Sci USA. 1980;77(1):518–521.
  • Camaschella C, Kattamis AC, Petroni D, Different hematological phenotypes caused by the interaction of triplicated α-globin genes and heterozygous β-thalassemia. Am J Hematol. 1997; 55(2):83–88.
  • Giordano PC, Bakker-Verwij M, Harteveld CL. Frequency of α-globin gene triplications and their interaction with β-thalassemia mutations. Hemoglobin. 2009;33(2):124–131.
  • Panigrahi I, Mahapatra M, Kumar R, Kumar G, Choudhry VP, Saxena R. Jaundice and α gene triplication in β-thalassemia: association or causation? Hematology. 2006;11(2):109–112.
  • Traeger-Synodinos J, Kanavakis E, Vrettou C, The triplicated α-globin gene locus in β-thalassaemia heterozygotes: clinical, haematological, biosynthetic and molecular studies. Br J Haematol. 1996;95(3):467–471.
  • Premawardhena A, Fisher CA, Olivieri NF, A novel molecular basis for β thalassemia intermedia poses new questions about its pathophysiology. Blood. 2005;106(9):3251–3255.
  • Carnley BP, Prior JF, Gilbert A, The prevalence and molecular basis of hemoglobinopathies in Cambodia. Hemoglobin. 2006;30(4):463–470.
  • Ma SK, Chan AY, Chan LC, Chui DHK, Waye JS. Compound heterozygosity for triplicated α-globin gene and (– –(SEA)) α-globin gene deletion: implication for thalassaemia screening. Br J Haematol. 2000;110(2):498–499.
  • Bergstrome JA, Poon A. Evaluation of a single-tube multiplex polymerase chain reaction screen for detection of common α-thalassemia genotypes in a clinical laboratory. Am J Clin Pathol. 2002; 118(1):18–24.
  • Chong SS, Boehm CD, Cutting GR, Higgs DR. Simplified multiplex-PCR diagnosis of common Southeast Asian deletional determinants of α-thalassemia. Clin Chem. 2000;46(10):1692–1695.
  • Tan AS, Quah TC, Low PS, Chong SS. A rapid and reliable 7-deletion multiplex polymerase chain reaction assay for α-thalassemia. Blood. 2001;98(1):250–251.
  • Liu YT, Old JM, Miles K, Fisher CA, Weatherall DJ, Clegg JB. Rapid detection of α-thalassaemia deletions and α-globin gene triplication by multiplex polymerase chain reactions. Br J Haematol. 2000;108(2):295–299.
  • Chong SS, Boehm CD, Higgs DR, Cutting GR. Single-tube multiplex-PCR screen for common deletional determinants of α-thalassemia. Blood. 2000;95(1):360–362.
  • Wang W, Ma ES, Chan AY, Single-tube multiplex-PCR screen for anti-3.7 and anti-4.2 α-globin gene triplications. Clin Chem. 2003;49(10):1679–1682.

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