192
Views
4
CrossRef citations to date
0
Altmetric
Original Article

An ELISA to Measure Mucosal IgA Specific for Bacteroides Surface Antigens in Whole Gut Lavage Fluid

, , , &
Pages 129-136 | Received 20 Jan 1995, Accepted 21 Apr 1995, Published online: 11 Jul 2009

References

  • Allan E, Poxton I R., Barclay G R. Antibacteroides lipopolysaccharide IgG levels in healthy adults and sepsis patients. FEMS Immunology Medical Microbiology 1995; 11: 5–12
  • Barclay G R., Scott B B. Serological relationships between Escherichia coli and Salmonella smooth-and rough-mutant lipopolysaccharides as revealed by enzyme-linked immunosorbent assay for human immunoglobulin G antiendotoxin antibodies. Infection and Immunity 1987; 55: 2706–2714
  • Berg R D., Savage D C. Immunological responses and microorganisms indigenous to the gastrointestinal tract. American Journal of Clinical Nutrition 1972; 25: 1364–1371
  • Brydon W G., Choudari C P., Ferguson A. Relative specificity for active inflammatory bowel disease of plasma-derived proteins in gut lavage fluid. European Journal of Gastroenterology and Hepatology 1993; 5: 269–273
  • Choudari C P., O'Mahony S, Brydon G, Mwantembe O, Ferguson A. Gut lavage fluid protein concentrations: objective measures of disease activity in inflammatory bowel disease. Gastroenterology 1993; 104: 1064–1071
  • Delahooke D M., Barclay G R., Poxton I R. A re-appraisal of the biological activity of Bacteroides lipopolysaccharide. Journal of Medical Microbiology 1995; 42: 102–112
  • Delahooke D M., Barclay G R., Poxton I R. Tumor necrosis factor induction by an aqueous phenol-extracted lipopolysaccharide complex from Bacteroides species. Infection and Immunity 1995; 63: 840–846
  • Ferguson A, Croft N, Humphreys K. Technical Report: Results of immunological tests on faecal extracts are likely to be extremely misleading. Clinical and Experimental Immunology 1995, (in press)
  • Ferguson A, Ghosh S, Handy L M., Ghoudari C, Mwantembe O, McIntyre M A. Analysis of disease distribution, activity and complications in the patient with inflammatory bowel disease. Scandinavian Journal of Gastroenterology 1994; 29: 15–19
  • Ferguson A, Sallam J. Mucosal immunity to oral vaccines. Lancet 1992; 339: 179
  • Ferguson A, Sallam J, McLintock L, Croft N, Poxton I R. The gut as an immune organ; intestinal anti-endotoxin antibodies. Organ Metabolism and Nutrition: Ideas for Future Critical Care, JM Kinney, HN Tucker. Raven Press, New York 1994; 231–244
  • Ferguson A, Sallam J, O'Mahoney S, Poxton I R. Clinical investigation of gut immune responses. Advanced Drug Delivery 1995, (in press)
  • Filip C, Fletcher G, Wulff J L., Earhart C F. Solubilization of the cytoplasmic membrane of Escherichia coli by the ionic detergent sodium lauroyl sarcosinate. Journal of Bacteriology 1973; 115: 717–722
  • Finegold S M. General aspects of anaerobic infection. Anaerobic Infections in Humans, SM Finegold, WL George. Academic Press. 1989; 137–153
  • Hancock I C., Poxton I R. Bacterial Cell Surface Techniques. 1st edn. Wiley, ChichesterUK 1988
  • Hodges M, Kingstone K, Brydon W G., Sallam J, Ferguson A. Use of gut lavage to measure intestinal immunity in healthy Sierra Leonean children. Journal of Pediatric Gastroenterology and Nutrition 1994; 19: 65–70
  • Holbrook W P., Duerden B I., Deacon A G. The classification of Bacteroides melaninogenicus and related species. Journal of Applied Bacteriology 1977; 42: 259–273
  • Lowry O H., Rosebrough N J., Farr A L., Randall R J. Protein measurement with the Folin Phenol reagent. Journal of Biological Chemistry 1951; 193: 265–275
  • Namavar F, Theunissen E BM, Verweij-Van Vught A MJJ, Peerbooms P GH, Bal M, Hoitsma H FW, Maclaren D M. Epidemiology of the Bacteroides fragilis group in the colonic flora in 10 patients with colonic cancer. Journal of Medical Microbiology 1989; 29: 171–176
  • O'Mahony S, Anderson N, Nuki G, Ferguson A. Systemic and mucosal antibodies to klebsiella in patients with ankylosing spondylitis and Crohn's disease. Annals of Rheumatic Diseases 1992; 51: 1296–1300
  • O'Mahony S, Arranz E, Barton J R., Ferguson A. Dissociation between systemic and mucosal humoral responses in coeliac disease. Gut 1991; 32: 29–35
  • O'Mahony S, Barton J R., Crichton S, Ferguson A. Appraisal of gut lavage in the study of intestinal humoral immunity. Gut 1990; 31: 1341–1344
  • Poxton I R., Brown R, Collee J G. Detection of species-specific and cross-reactive cell surface antigens of Bacteroides species by an indirect enzyme-linked immunosorbent assay. Journal of Medical Microbiology 1982; 15: 223–231
  • Sallam J. Intestinal humoural immunity in man: IgA and anti-salmonella antibodies. PhD thesis. University of Edinburgh. 1995
  • Scott B B., Barclay G R. Endotoxin-polymyxin complexes in an improved enzymelinked immunosorbent assay for IgG antibodies in blood donor sera to gram-negative endotoxin core glycolipids. Vox Sanguinis 1987; 52: 272–280
  • Tzianabos A O., Onderdonk A B., Rosner B, Cisneros R L., Kasper D L. Structural features of polysaccharides that induce intraabdominal abscesses. Science 1993; 262: 416–419