17
Views
33
CrossRef citations to date
0
Altmetric
Original Article

Ligand-Induced Homo-and Hetero-Dimerization of Platelet-Derived Growth Factor α- and β-Receptors in Intact Cells

, , , &
Pages 1-14 | Received 09 Aug 1991, Accepted 22 Aug 1991, Published online: 11 Jul 2009

References

  • Bishayee S., Majumdar S., Khire J., Das M. Ligand‐induced dimerization of the platelet‐derived growth factor receptor. Monomer‐dimer interconversion occurs independent of receptor phosphorylation. J. Biol. Chem. 1989; 264: 11699–11705
  • Blobel G., Dobberstein B. Transfer of proteins across membranes. II. Reconstitution of functional rough microsomes from heterologous components. J. Cell. Biol. 1975; 67: 835–851
  • Bowen‐Pope D. F., Hart C. E., Seifert R. A. Sera and conditioned media contain different isoforms of platelet‐derived growth factor (PDGF) which bind to different classes of PDGF receptor. J. Biol. Chem. 1989; 264: 2502–2508
  • Claesson‐Welsh L., Eriksson A., Moren A., Severinsson L., Ek B., Östman A., Betsholtz C., Heldin C. ‐H. cDNA cloning and expression of a human platelet‐derived growth factor (PDGF) receptor specific for B‐chain‐containing PDGF molecules. Mol. Cell. Biol. 1988; 8: 3476–3486
  • Claesson‐Welsh L., Eriksson A., Westermark B., Heldin C. ‐H. cDNA cloning and expression of the human A‐type platelet‐derived growth factor (PDGF) receptor establishes structural similarity to the B type PDGF receptor. Proc. Natl. Acad. Sci. USA 1989a; 86: 4917–4921
  • Claesson‐Welsh L., Hammacher A., Westermark B., Heldin C. ‐H., Nister M. Identification and structural analysis of the A type receptor for platelet‐derived growth factor: Similarities with the B type receptor. J. Biol. Chem. 1989b; 264: 1742–1747
  • Glenn K., Bowen‐Pope D. F., Ross R. Platelet‐derived Growth Factor III. Identification of a platelet‐derived growth factor receptor by affinity labeling. J. Biol. Chem. 1982; 257: 5172–5176
  • Gronwald R. G. K., Grant F. J., Haldeman B. A., Hart C. E., O'Hara P. J., Hagen F. S., Ross R., Bowen‐Pope D. F., Murray M. J. Cloning and expression of a cDNA coding for the human platelet‐derived growth factor receptor: evidence for more than one receptor class. Proc. Natl. Acad. Sci. USA 1988; 85: 3435–3439
  • Hammacher A., Nistér M., Westermark B., Heldin C. ‐H. A human glioma cell line secretes three structurally and functionally different dimeric forms of platelet‐derived growth factor. Eur. J. Biochem. 1988a; 176: 179–186
  • Hammacher A., Hellman U., Johnsson A., Östman A., Gunnarson K., Westermark B., Wasteson A., Heldin C. ‐H. A major part of platelet‐derived growth factor purified from human platelets is a heterodimer of one A and one B chain. J. Biol. Chem. 1988b; 263: 16493–16498
  • Hammacher A., Mellström K., Heldin C. ‐H., Westermark B. Isoform‐specific induction of actin reorganization by platelet‐derived growth factor suggests that the functionally active receptor is a dimer. EMBO J. 1989; 8: 2489–2495
  • Heldin C. ‐H., Ek B., Rönnstrand L. Characterization of the receptor for platelet‐derived growth factor on human fibroblasts. Demonstration of an intimate relationship with a 185,000 dalton substrate for the platelet‐derived growth factor‐stimulated kinase. J. Biol. Chem. 1983; 258: 10054–10061
  • Heldin C. ‐H., Johnsson A., Wennergren S., Wernstedt C., Betsholtz C., Westermark B. A human osteosarcoma cell line secretes a growth factor structurally related to a homodimer of PDGF A chains. Nature 1986; 319: 511–514
  • Heldin C. ‐H., Backström G., Östman A., Hammacher A., Ronnstrand L., Rubin K., Nister M., Westermark B. Binding of different dimeric forms of PDGF to human fibroblasts: evidence for two separate receptor types. EMBO J. 1988; 7: 1387–1394
  • Heldin C. ‐H., Ernlund A., Rorsman C., Rönnstrand L. Dimerization of B‐type platelet‐derived growth factor receptors occurs after ligand binding and is closely associated with receptor kinase activation. J. Biol. Chem. 1989; 264: 8905–8912
  • Heldin C. ‐H., Westermark B. Platelet‐derived growth factor: mechanism of action and possible in vivo function. Cell Regulation 1990; 1: 555–566
  • Kankaraj P., Raj S., Khan S. A., Bishayee S. Ligand‐induced interaction between α‐ and β‐type platelet derived growth factor (PDGF) receptors: Role of receptor heterodimers in kinase activation. Biochemistry 1991; 30: 1761–1767
  • Kazlauskas A., Cooper J. A. Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins. Cell 1989; 58: 1121–1133
  • Kelly J. D., Haldeman B. A., Grant F. J., Murray M. J., Seifert R. A., Bowen‐Pope D. F., Cooper J. A., Kazlauskas A. Platelet‐derived growth factor (PDGF) stimulates PDGF receptor subunit dimerization and intersubunit transphosphorylation. J. Biol. Chem. 1991; 266: 8987–8992
  • Li W., Schlessinger J. Platelet‐derived growth factor (PDGF)‐induced disulphide‐linked dimerization of PDGF receptor in living cells. Mol. Cell. Biol. 1991; 11: 3756–3761
  • Matsui T., Heidaran M., Miki T., Toru M., Popescu N., La Rochelle W., Kraus M., Pierce J., Aaronson S. A. Isolation of a novel receptor cDNA establishes the existence of two PDGF receptor genes. Science 1989; 243: 800–803
  • Östman A., Rail L., Hammacher A., Wormstead M. A., Coit D., Valenzuela P., Betsholtz C., Westermark B., Heldin C. ‐H. Synthesis and assembly of a functionally active recombinant platelet‐derived growth‐factor AB heterodimer. J. Biol. Chem. 1988; 263: 16202–16208
  • Östman A., Bäckström G., Fong N., Betsholtz C., Wernstedt C., Hellman U., Westermark B., Valenzuela P., Heldin C. ‐H. Expression of three recombinant homodimeric isoforms of PDGF in Saccharomyces cerevisiae: Evidence for difference in receptor binding and functional activities. Growth Factors 1989; 1: 271–281
  • Raines E. W., Bowen‐Pope D. F., Ross R. Peptide growth factors and their receptors. Handbook of Experimental Pharmacology, M. B. Sporn, A. B. Roberts. Springer‐Verlag, Heidelberg 1990; Vol. 95: 173–262, part I
  • Rönnstrand L., Terracio L., Claesson‐Welsh L., Heldin C. ‐H., Rubin K. Characterization of two monoclonal antibodies reactive with the external domain of the platelet‐derived growth factor receptor. J. Biol. Chem. 1988; 263: 10429–10435
  • Seifert R. A., Hart C. E., Philips P. E., Forstrom J. W., Ross R., Murray M., Bowen‐Pope D. F. Two different subunits associate to create isoform‐specific platelet‐derived growth factor receptors. J. Biol. Chem. 1989; 264: 8771–8778
  • Stroobant P., Waterfield M. D. Purification and properties of porcine platelet‐derived growth factor. EMBO J. 1984; 3: 2963–2967
  • Westermark B., Siegbahn A., Heldin C. ‐H., Claesson‐Welsh L. B‐type receptor for platelet‐derived growth factor mediates a chemotactic response by means of ligand‐induced activation of the receptor protein‐tyrosine kinase. Proc. Natl. Acad. Sci. USA 1990; 87: 128–132
  • Yarden Y., Escobedo J. A., Kuang W. ‐J., Yang‐Feng T. L., Daniel T. O., Tremble P. M., Chen E. Y., Ando M. E., Harkins R. N., Francke U., Friend V. A., Ullrich A., Williams L. T. Structure of the receptor for platelet‐derived growth factor helps define a family of closely related growth factor receptors. Nature 1986; 323: 226–232

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.