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Original Article

Platelet-Derived Growth Factor Induces Phosphatidylinositol 3-Kinase Release from the Middle T-pp60c-src Complex and Association with the Platelet-Derived Growth Factor Receptor

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Pages 41-51 | Received 07 Jul 1993, Accepted 17 Sep 1993, Published online: 11 Jul 2009

References

  • Auger K. R., Carpenter C. L., Shoelson S. E., Piwnica-Worms H., Cantley L. C. Polyoma virus middle T antigen-pp60c-src complex associates with purified phosphatidylinositol 3-kinase in vitro. J. Biol. Chem. 1992; 267: 5408–5415
  • Burgess A. W., Lloyd C. J., Nice E. C. Murine epidermal growth factor: heterogeneity on high resolution ion-exchange chromatography. EMBO J. 1983; 2: 2065–2069
  • Carpenter C. L., Auger K. R., Chanudhuri M., Yoakim M., Schaffhausen B., Shoelson S., Cantley L. C. Phosphoinositide 3-kinase is activated by phosphopeptides that bind to the SH2 domains of the 85-kDa subunit. J. Biol. Chem. 1993; 268: 9478–9483
  • Carpenter C. L., Cantley L. C. Phosphoinositide kinases. Biochemistry 1990; 29: 11147–11156
  • Carpenter C. L., Duckworth B. C., Auger K. R., Cohen B., Schaffhausen B. S., Cantley L. C. Purification and characterization of phosphoinositide 3-kinase from rat liver. J. Biol. Chem. 1990; 265: 19704–19711
  • Cartwright C. A., Kaplan P. L., Cooper J. A., Hunter T., Eckhart W. Altered sites of tyrosine phosphorylation in pp60c-src associated with polyomavirus middle tumour antigen. Mol. Cell. Biol. 1986; 6: 1562–1570
  • Cheng S. H., Piwnica-Worms H., Harvey R. W., Roberts T. M., Smith A. E. The carboxy terminus of pp60c-src is a regulatory domain and is involved in complex formation with the middle-T antigen of polyomavirus. Mol. Cell. Biol. 1988; 8: 1736–1747
  • Cherington V., Morgan B., Spiegelman B. M., Roberts T. M. Recombinant retroviruses that transduce individual polyoma tumor antigens: Effects on growth and differentiation. Proc. Natl Acad. Sci. USA 1986; 83: 4307–4311
  • Cleveland D. W. Peptide mapping in one dimension by limited proteolysis of sodium dodecyl sulfate-solubilized proteins. Meth. Enzymol. 1983; 96: 222–229
  • Cohen B., Liu Y., Druker B., Roberts T. M., Schaffhausen B. S. Characterization of pp85, a target of oncogenes and growth factor receptors. Mol. Cell. Biol. 1990a; 10: 2909–2915
  • Cohen B., Yoakim M., Piwnica-Worms H., Roberts T. M., Schaffhausen B. S. Tyrosine phosphorylation is a signal for the trafficking of pp85, an 85-kDa phosphorylated polypeptide associated with phosphatidylinositol kinase activity. Proc. Natl Acad. Sci. USA 1990b; 87: 4458–4462
  • Coughlin S. R., Escobedo J. A., Williams L. T. Role of phosphatidylinositol kinase in PDGF receptor signal transduction. Science 1989; 243: 1191–1194
  • Courtneidge S. A. Activation of pp60c-src kinase by middle T antigen binding or by dephosphorylation. EMBO J. 1985; 4: 1471–1477
  • Courtneidge S. A., Heber A. An 81 kDa protein complexed with middle T antigen and pp60c-src A possible phosphatidylinositol kinase. Cell 1987; 50: 1031–1037
  • Courtneidge S. A., Read M., Wilson J. B., Fried M. Cytoplasmic interaction between pp60c-src and a truncated polyoma virus middle T antigen. Oncogene Res. 1989; 4: 75–80
  • Decker S. J. Transmembrane signalling by epidermal growth factor receptors lacking autophosphorylation sites. J. Biol. Chem. 1993; 268: 9176–9179
  • Druker B. J., Ling L. E., Cohen B., Roberts T. M., Schaffhausen B. S. A completely transformation-defective point mutant of polyomavirus middle T antigen which retains full associated phosphatidylinositol kinase activity. J. Virol. 1990; 64: 4454–4461
  • Escobedo J. A., Navankasattusas S., Kavanaugh W. M., Milfay D., Fried V. A., Williams L. T. cDNA cloning of a novel 85 kDa protein that has SH2 domains and regulates binding of P13-kinase to the PDGF β-receptor. Cell 1991; 65: 75–82
  • Fantl W. J., Escobedo J. A., Martin G. A., Turck C. W., del Rosario M., McCormick F., Williams L. T. Distinct phosphotyrosines on a growth factor receptor bind to specific molecules that mediate different signalling pathways. Cell 1992; 69: 413–423
  • Gould K. L., Hunter T. Platelet-derived growth factor induces multisite phosphorylation of pp60c-src and increases its protein-tyrosine kinase activity. Mol. Cell. Biol. 1988; 8: 3345–3356
  • Hiles I. D., Otsu M., Volinia S., Fry M. J., Gout I., Dhand R., Panayotou G., Ruiz-Larrea F., Thompson A., Totty N. F., Hsuan J. J., Courtneidge S. A., Parker P. J., Waterfield M. D. Phosphatidylinositol 3-kinase: Structure and expression of the 110 kDa catalytic subunit. Cell 1992; 70: 419–429
  • Hunter T., Hutchinson M. A., Eckhart W. Polyoma middle-sized T antigen can be phosphorylated on tyrosine at multiple sites in vitro. EMBO J. 1984; 3: 73–79
  • Kashishian A., Kazlauskas A., Cooper J. A. Phosphorylation sites in the PDGF receptor with different specificities for binding GAP and P13 kinase in vivo. EMBO J. 1992; 11: 1373–1382
  • Kavanaugh W. M., Klippel A., Escobedo J. A., Williams L. T. Modification of the 85-kDa subunit of phosphatidylinositol-3 kinase in platelet-derived growth factor-stimulated cells. Mol. Cell. Biol. 1992; 12: 3415–3424
  • Kazlauskas A., Cooper J. A. Autophosphorylation of the PDGF receptor in the kinase insert region regulates interactions with cell proteins. Cell 1989; 58: 1121–1133
  • Kazlauskas A., Cooper J. A. Phosphorylation of the PDGF receptor β subunit creates a tight binding site for phosphatidylinositol 3 kinase. EMBO J. 1990; 9: 3279–3286
  • Klippel A., Escobedo J. A., Fantl W. J., Williams L. T. The C-terminal SH2 domain of p85 accounts for the high affinity and specificity of the binding of phosphatidylinositol 3-kinase to phosphorylated platelet-derived growth factor β receptor. Mol. Cell. Biol. 1992; 12: 1451–1459
  • Kypta R. M., Goldberg Y., Ulug E. T., Courtneidge S. A. Association between the PDGF receptor and members of the src family of tyrosine kinases. Cell 1990; 62: 481–492
  • Ling L. E., Druker B. J., Cantley L. C., Roberts T. M. Transformation-defective mutants of polyomavirus middle T antigen associate with phosphatidylinositol 3-kinase (PI 3-kinase) but are unable to maintain wild-type levels of PI 3-kinase products in intact cells. J. Virol. 1992; 66: 1702–1708
  • Majerus P. W., Ross T. S., Cunningham T. W., Caldwell K. K., Jefferson A. B., Bansal V. S. Recent insights in phosphatidylinositol signalling. Cell 1990; 63: 459–465
  • Markland W., Oostra B. A., Harvey R., Markham A. F., Colledge W. H., Smith A. E. Site-directed mutagenesis of polyomavirus middle-T antigen sequences encoding tyrosine 315 and tyrosine 250. J. Virol. 1986; 59: 384–391
  • McGlade C. J., Ellis C., Reedijk M., Anderson D., Mbamalu G., Reith A. D., Panayotou G., End P., Bernstein A., Kazlauskas A., Waterfield M. D., Pawson T. SH2 domains of the p85α subunit of phosphatidylinositol 3-kinase regulate binding to growth factor receptors. Mol Cell. Biol. 1992; 12: 991–997
  • Novak U., Griffin B. E. Requirement for the C-terminal region of middle T-antigen in cellular transformation by polyoma virus. Nucl. Acids Res. 1981; 9: 2055–2073
  • Otsu M., Hiles I., Gout I., Fry M. J., Ruiz-Larrea F., Panayotou G., Thompson A., Dhand R., Hsuan J., Totty N., Smith A. D., Morgan S. J., Courtneidge S. A., Parker P. J., Waterfield M. D. Characterization of two 85 kDa proteins that associate with receptor tyrosine kinases, middle-T/pp60c-src complexes, and PI 3-kinase. Cell 1991; 65: 91–104
  • Pallas D. C., Shahrik L. K., Martin B. L., Jaspers S., Miller T. B., Brautigan D. L., Roberts T. M. Polyoma small and middle T antigens and SV40 small t antigen form stable complexes with protein phosphatase 2A. Cell 1990; 60: 167–176
  • Rake J. B., Quinones M. A., Faller D. V. Inhibition of platelet-derived growth factor-mediated signal transduction by transforming ras. J. Biol. Chem. 1991; 266: 5348–5352
  • Read S. M., Northcote D. H. Minimization of variation in the response to different proteins of the Coomassie Blue G dye-binding assay for protein. Anal Biochem. 1981; 116: 53–64
  • Roussel M. F., Shurtleff S. A., Downing J. R., Sherr C. J. A point mutation at tyrosine-809 in the human colony-stimulating factor 1 receptor impairs mitogenesis without abrogating tyrosine kinase activity, association with phosphatidylinositol 3-kinase, or induction of c-fos and junB genes. Proc. Natl Acad. Sci. USA 1990; 87: 6738–6742
  • Segawa K. Cooperation of mitogenic growth factors with polyoma virus middle T antigen in transformation of secondary cultured rat cells. Biochem. Biophys. Res. Commun. 1986; 136: 921–926
  • Segawa K., Ito Y. Enhancement of polyoma virus middle T antigen tyrosine phosphorylation by epidermal growth factor. Nature 1983; 304: 742–744
  • Shibasaki F., Homma Y., Takenawa T. Two types of phosphatidylinositol 3-kinase from bovine thymus. Monomer and heterodimer form. J. Biol. Chem. 1991; 266: 8108–8114
  • Skolnik E. Y., Margolis B., Mohammadi M., Lowenstein E., Fischer R., Drepps A., Ullrich A., Schlessinger J. Cloning of P13 kinase-associated p85 utilizing a novel method for expression/cloning of target proteins for receptor tyrosine kinases. Cell 1991; 65: 83–90
  • Susa M., Keeler M., Varticovski L. Platelet-derived growth factor activates membrane-associated phosphatidylinositol 3-kinase and mediates its translocation from the cytosol. Detection of enzyme activity in detergent-solubilized cell extracts. J. Biol. Chem. 1992; 267: 22951–22956
  • Talmage D. A., Freund R., Young A. T., Dahl J., Dawe C. J., Benjamin T. L. Phosphorylation of middle T by pp60c-src: A switch for binding of phosphatidylinositol 3-kinase and optimal tumorigenesis. Cell 1989; 59: 55–65
  • Ullrich A., Schlessinger J. Signal transduction by receptors with tyrosine kinase activity. Cell 1990; 61: 203–212
  • Ulug E. T., Cartwright A. J., Courtneidge S. A. Characterization of the interaction of polyomavirus middle T antigen with type 2A protein phosphatase. J. Virol. 1992; 66: 1458–1467
  • Valius M., Kazlauskas A. Phospholipase C-γ1 and phosphatidylinositol 3 kinase are the downstream mediators of the PDGF receptor's mitogenic signal. Cell 1993; 73: 321–334
  • Walker F., Burgess A. W. Reconstitution of the high affinity epidermal growth factor receptor on cell-free membranes after transmodulation by platelet-derived growth factor. J. Biol Chem. 1991; 226: 2746–2752
  • Walter G., Ruediger R., Slaughter C., Mumby M. Association of protein phosphatase 2A with polyoma virus medium tumor antigen. Proc. Natl Acad. Sci. USA 1990; 87: 2521–2525
  • Whitman M., Kaplan D. R., Schaffhausen B., Cantley L., Roberts T. M. Type I phosphatidylinositol kinase makes a novel phospholipid, phosphatidylinositol-3-phosphate. Nature 1985; 315: 239–242
  • Whitman M., Downes C. P., Keeler M., Keller T., Cantley L. Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation. Nature 1988; 332: 644–646
  • Yoakim M., Hou W., Liu Y., Carpenter C. L., Kapeller R., Schaffhausen B. S. Interactions of polyomavirus middle T with the SH2 domains of the pp85 subunit of phosphatidylinositol-3-kinase. J. Virol. 1992; 66: 5485–5491

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