A semi-automated micronucleus-centromere assay to assess low-dose radiation exposure in human lymphocytes

References

• Ainsbury EA, Lloyd DC. 2010. Dose estimation software for radiation biodosimetry. Health Physics 98:290–295.
   PubMed Web of Science ®Google Scholar
• Bolognesi C, Lando C, Forni A, Landini E, Scarpato R, Migliore L, Bonassi S. 1999. Chromosomal damage and ageing: Effect on micronuclei frequency in peripheral blood lymphocytes. Age and Ageing 28:393–397.
   PubMed Web of Science ®Google Scholar
• Catalan J, Autio K, Kuosma E, Norppa H. 1998. Age-dependent inclusion of sex chromosomes in lymphocyte micronuclei of man. American Journal of Human Genetics 63:1464–1472.
   PubMedGoogle Scholar
• Carere A, Antoccia A, Cimini D, Crebelli R, Degrassi F, Leopardi P, Marcon F, Sgura A, Tanzarella C, Zijno A. 1999. Analysis of chromosome loss and non-disjunction in cytokinesis-blocked lymphocytes of 24 male subjects. Mutagenesis 14:491–496.
   PubMed Web of Science ®Google Scholar
• Cho YH, Kim YJ, An YS, Woo HD, Choi SY, Kang CM, Chung HW. 2009. Micronucleus-centromere assay and DNA repair gene polymorphism in lymphocytes of industrial radiographers. Mutation Research 680:17–24.
   PubMed Web of Science ®Google Scholar
• Darroudi F, Meijers CM, Hadjidekova V, Natarajan AT. 1996. Detection of aneugenic and clastogenic potential of X-rays, directly and indirectly acting chemicals in human hepatoma (Hep G2) and peripheral blood lymphocytes, using the micronucleus assay and fluorescent in situ hybridization with a DNA centromeric probe. Mutagenesis 11:425–433.
   PubMed Web of Science ®Google Scholar
• Dias FL, Antunes LMG, Rezende PA, Carvalho FES, Silva CMD, Matheus JM, Oliveira JV, Lopes GP, Pereira GA, Balarin MAS. 2007. Cytogenetic analysis in lymphocytes from workers occupationally exposed to low levels of ionizing radiation. Environmental Toxicology and Pharmacology 23:228–233.
   PubMed Web of Science ®Google Scholar
• Fenech M, Morley AA. 1985. Measurement of micronuclei in lymphocytes. Mutation Research 147:29–36.
   PubMed Web of Science ®Google Scholar
• Fenech M, Neville S, Rinaldi J. 1994. Sex is an important variable affecting spontaneous micronucleus frequency in cytokinesis-blocked lymphocytes. Mutation Research 313:203–207.
   PubMed Web of Science ®Google Scholar
• Fenech M. 1998. Important variables that influence base-line micronucleus frequency in cytokinesis-blocked lymphocytes – a biomarker for DNA damage in human populations. Mutation Research 404:155–165.
   PubMed Web of Science ®Google Scholar
• Huber R, Salassidis K, Kulka U, Braselmann H, Bauchinger M. 1996. Detection of centromeres in vinblastine- and radiation-induced micronuclei of human lymphocytes using FISH with an alpha satellite pancentromeric DNA probe. Environmental and Molecular Mutagenesis 27:105–109.
   PubMedGoogle Scholar
• Lindberg HK, Falck GC, Jarventaus H, Norppa H. 2008. Characterization of chromosomes and chromosomal fragments in human lymphocyte micronuclei by telomeric and centromeric FISH. Mutagenesis 23:371–376.
   PubMedGoogle Scholar
• Muller WU, Streffer C. 1991. Biological indicators for radiation damage. International Journal of Radiation Biology 59:863–873.
   PubMed Web of Science ®Google Scholar
• Norppa H, Renzi L, Lindholm C. 1993. Detection of whole chromosomes in micronuclei of cytokinesis-blocked human lymphocytes by antikinetochore staining and in situ hybridization. Mutagenesis 8:519–525.
   PubMedGoogle Scholar
• Norppa H, Falck GCM. 2003. What do human micronuclei contain? Mutagenesis 18:221–233.
   PubMed Web of Science ®Google Scholar
• Pala FS, Alkaya F, Tabakcioglu K, Tokatli F, Uzal C, Parlar S, Algunes C. 2008. The effects of micronuclei with whole chromosome on biological dose estimation. Turkish Journal of Biology 32:283–290.
   Web of Science ®Google Scholar
• Romm H, Oestreicher U, Kulka U. 2009. Cytogenetic damage analysed by the dicentric assay. Annali dell'Istituto Superiori di Sanita 45:251–259.
   PubMed Web of Science ®Google Scholar
• Sari-Minodier I, Orsiere T, Bellon L, Pompili J, Sapin C, Botta A. 2002. Cytogenetic monitoring of industrial radiographers using the micronucleus assay. Mutation Research 521:37–46.
   PubMed Web of Science ®Google Scholar
• Sari-Minodier I, Orsiere T, Auquier P, Martin F, Botta A. 2007. Cytogenetic monitoring by use of the micronucleus assay among hospital workers exposed to low doses of ionizing radiation. Mutation Research 629:111–121.
   PubMed Web of Science ®Google Scholar
• Schunck C, Johannes T, Varga D, Lorch T, Plesch A. 2004. New developments in automated cytogenetic imaging: unattended scoring of dicentric chromosomes, micronuclei, single cell gel electrophoresis, and fluorescence signals. Cytogenetic and Genome Research 104:383–389.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, De Ridder L. 1991. Biological dosimetry using the micronucleus assay for lymphocytes: Interindividual differences in dose-response. Health Physics 61:623–630.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, De Ridder L. 1996. A cytogenetic study of radiological workers: Effect of age, smoking and radiation burden on the micronucleus frequency. Mutation Research 360:75–82.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, De Ridder L, Touil N, Kirsch-Volders M, Lambert V, Laurent C. 1999a. Inter-laboratory comparison of cytogenetic endpoints for the biomonitoring of radiological workers. International Journal of Radiation Biology 75:23–34.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, Barbe M, Aousalah B, De Ridder L. 1999b. A cytogenetic study of nuclear power plant workers using the micronucleus-centromere assay. Mutation Research 445:105–111.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, Morthier R, Aousalah B, De Ridder L. 2000. Cytogenetic monitoring of hospital workers occupationally exposed to ionising radiation using the micronucleus centromere assay. Mutagenesis 15:245–249.
   PubMed Web of Science ®Google Scholar
• Thierens H, Vral A, Barbe M, De Ridder L. 2002. Micronucleus assay reveals no radiation effects among nuclear power plant workers. Health Physics 83:178–182.
   PubMedGoogle Scholar
• Varga D, Johannes T, Jainta S, Schwarz-Boeger U, Kiechle M, Garcia BP, Vogel W. 2004. An automated scoring procedure for the micronucleus test by image analysis. Mutagenesis 19:391–397.
   PubMed Web of Science ®Google Scholar
• Vaurijoux A, Gruel G, Pouzoulet F, Gregoire E, Martin C, Rochlefevre S, Voisin P, Roy L. 2009. Strategy for population triage based on dicentric analysis. Radiation Research 171: 541–548.
   PubMed Web of Science ®Google Scholar
• Vral A, Verhaegen F, Thierens H, de Ridder L. 1994. The in vitro cytokinesis-block micronucleus assay: A detailed description of an improved slide preparation technique for the automated detection of micronuclei in human lymphocytes. Mutagenesis 9: 439–443.
   PubMed Web of Science ®Google Scholar
• Vral A, Thierens H, De Ridder L. 1997. In vitro micronucleus-centromere assay to detect radiation-damage induced by low doses in human lymphocytes. International Journal of Radiation Biology 71:61–68.
   PubMed Web of Science ®Google Scholar
• Willems P, August L, Slabbert J, Romm H, Oestreicher U, Thierens H, Vral A. 2010. Automated micronucleus (MN) scoring for population triage in case of large scale radiation events. International Journal of Radiation Biology 86:2–11.
   PubMed Web of Science ®Google Scholar
• Weier HU, Lucas JN, Poggensee M, Segraves R, Pinkel D, Gray JW. 1991. Two-color hybridization with high complexity chromosome-specific probes and a degenerate alpha satellite probe DNA allows unambiguous discrimination between symmetrical and asymmetrical translocations. Chromosoma 100:371–379.
   PubMed Web of Science ®Google Scholar
• Wojcik A, Kowalska M, Bouzyk E, Buraczewska I, Kobialko G, Jarocewicz N, Szumiel I. 2000. Validation of the micronucleus-centromere assay for biological dosimetry. Genetics and Molecular Biology 23:1083–1085.
   Web of Science ®Google Scholar