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Biocatalysis Volume 7, 1993 - Issue 3
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Original Article

Reaction Kinetics of Immobilized α-Chymotrypsin in Organic Media 1. Influence at Solvent Polarity

Ernst Wehtje Dept. of Biotechnology, Univ. of Lund, PO Box 124, S-221 00, Lund, Sweden
,
Patrick Adlercreutz Dept. of Biotechnology, Univ. of Lund, PO Box 124, S-221 00, Lund, Sweden
&
Bo Mattiasson Dept. of Biotechnology, Univ. of Lund, PO Box 124, S-221 00, Lund, Sweden
Pages 149-161 | Received 19 May 1992, Accepted 28 Aug 1992, Published online: 11 Jul 2009

References

  • Blanco R. M., Hailing P. J., Bastida A., Cuesta C., Guisan J. M. Effect of immiscible organic solvents on activity/stability of native chymotrypsin and immobilized‐stabilized derivatives. Biotechnol. Bioeng 1992; 39: 75–84
  • Carrea G., Colorabi F., Mazzola G., Cremonesi P., Antonini E. Immobilized hydroxysteroid dehydrogenases for the transformation of steroids in water‐organic solvent systems. Biotechnol. Bioeng 1979; 21: 39–48
  • Cassells J. M., Hailing P. J. Protease‐catalyzed peptide synthesis in low‐water organic two‐phase systems and problems affecting it. Biotechnol. Bioeng 1989; 33: 1489–1494
  • Cremonesi P., Carrea G., Ferrara L., Antonini E. Enzymatic preparation of 20β‐hydroxysteroids in a two‐phase system. Biotechnol. Bioeng 1975; 17: 1101–1108
  • Estrada P., Sanchez‐Muniz R., Acebal C., Arche R., Castillon M. Characterization and optimization of immobilized polyphenol oxidase in low‐water organic solvents. Biotechn. Appl. Biochem 1991; 14: 12–20
  • Hailing P. J. Effects of water on equilibria catalysed by hydrolytic enzymes in biphasic reaction systems. Enzyme Microb. Technol 1984; 6: 513–516
  • Hailing P. J. Solvent selection for biocatalysis in mainly organic systems: predictions of effects on equilibrium position. Biotechnol. Bioeng 1990; 35: 691–701
  • Hilhorst M. H., Spruyt R., Laane C., Veeger C. Rules for the regulation of enzyme activity in reversed micelles as illustrated by the conversion of apolar steroids by 20β‐ hydroxysteroid dehydrogenase. Eur. J. Biochem 1984; 144: 459–466
  • Kasche V., Michaelis G., Galunsky B. Binding of organic solvent molecules influences the P1′‐P2′ stereo‐ and sequence‐specificity of α‐chymotrypsin in kinetically controlled peptide synthesis. Biotechnol. Lett 1991; 13: 75–80
  • Kazandjian R. Z., Klibanov A. M. Regioselective oxidation of polyphenols catalyzed by phenol oxidase in chloroform. J. Am. Chem. Soc 1985; 107: 5448–5450
  • Kemp G., Dickinson M., Ratledge C. Activity and substrate specificity of the fatty alcohol oxidase of Candida tropicalis in organic solvents. Appl. Microbiol. Biotechnol 1991; 34: 441–445
  • Laane C., Boeren S., Vos K. V. C. Rules for Optimization of biocatalysis in organic solvents. Biotechnol. Bioeng 1987; 30: 81–87
  • Reslow M., Adlercreutz P., Mattiasson B. Organic solvents for bioorganic synthesis. Appl. Microbiol. Biotechnol 1987; 26: 1–8
  • Reslow M. Enzyme as catalysts in organic media. PhD. thesis, Lund University. 1989
  • Riddick J. A., Bunger W. B., Sakano T. K. Organic solvents. Physical properties and methods of purification4th ed. Wiley, New York 1986
  • Rupley J. A., Gratton E., Careri G. Water and globular proteins. TIBS 1983; 18–22
  • Stevenson D., Storer A. Papain in organic solvents: determination of conditions suitable for biocatalysis and the effect on substrate specificity and inhibition. Biotechnol. Bioeng 1991; 37: 519–527
  • Valivety R. H., Hailing P. J., Macrae A. R. Reaction rate with suspended lipase catalyst shows similar dependence on water activity in different organic solvents. Biochim. Biophys. Acta 1992; 1118: 218–222
  • Valivety R. H., Johnston G. A., Suckling C. J., Hailing P. J. Solvent effects on biocatalysis in organic systems: equilibrium position and rates of lipase catalyzed esterification. Biotechnol. Bioeng 1991; 38: 1137–1143
  • Wehtje E., Adlercreutz P., Mattiasson B. Formation of C—C bonds by mandelonitrile lyase in organic solvents. Biotechnol. Bioeng 1990; 36: 39–46
  • Wehtje E., Adlercreutz P., Mattiasson B. Reaction kinetics of immobilized α‐chymotrypsin in organic media. 2. Effect of solvent polarity. Biocatalysis 1993; 7: 163–176
  • Yamane T., Kojima Y., Ichiryu T., Nagata M., Shimizu S. Intramolecular esterification by lipase powder in microaqueous benzene: effects of moisture content. Biotechol. Bioeng 1988; 34: 838–843
  • Zaks A., Klibanov A. M. Enzymatic catalysis in organic media at 100°C. Science 1984; 224: 1249–1251
  • Zaks A., Klibanov A. M. Enzyme‐catalyzed processes in organic solvents. Proc. Natl. Acad. Sci. U.S.A 1985; 82: 3192–3196
  • Zaks A., Klibanov A. M. Enzymatic catalysis in nonaqueous solvents. J. Biol. Chem 1988a; 263: 3194–3201
  • Zaks A., Klibanov A. M. The effect of water on enzyme action in organic media. J. Biol. Chem 1988b; 263: 8017–8021

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