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Original Article

Peptide Synthesis by Papain in Alkali Halide Media

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Pages 255-269 | Published online: 11 Jul 2009

References

  • Arakawa T., Timasheff S. N. Preferential interactions of proteins with salts in concentrated solutions. Biochemistry 1982; 23: 6545–6550
  • Arnon R. Papain. Methods Enzymol 1970; 19: 226–244
  • Combes D., Ye W. N., Zwick A., Monsan P. Effect of salts on enzyme stability. Ann. N.Y. Acad. Sci 1988; 542: 59–62
  • Gupta M. N. Enzyme function in organic solvents. Eur. J. Biochem 1992; 203: 25–32
  • Hahn-Hägerdal B. Water activity: a possible external regulator in biotechnological processes. Enzyme Microb. Technol 1986; 8: 322–327
  • Henley J. P., Sadana A. Categorization of enzyme deactivation using a series type mechanism. Enzyme Microb. Technol 1985; 7: 50–60
  • Hernández-Arana A., Soriano-Garcia M. Detection and characterisation by circular dichroism of a stable intermediate state formed in thermal unfolding of papain. Biochim. Biophys. Acta 1988; 954: 170–175
  • Kamp R. M. High-sensitivity amino acid analysis using high performance liquid chromatography and precolumn derivatization. LC-GC, Int 1991; 4: 40–46
  • Kasche V. Proteases in peptide synthesis. Proteolytic enzymes: A practical approach, R. J. Beynon, J. S. Bond. IRL Press, New York 1989; 125–143
  • Kullmann W. Advantages of enzymatic peptide synthesis. Enzyme Peptide Synthesis, W. Kullman. CRC Press, Inc., Boca Raton, Florida 1987; 37–40
  • Lozano P., Iborra J. L., Manjón A., Combes D. One step synthesis of Gly-Gly-PheNH2 from N-unprotected amino acid derivates by papain in one-phase-liquid media. Bioctechnol. Lett 1992; 14: 933–936
  • Lozano P., Combes D. Effect of alkali halides on α-chymotrypsin activity in the plastein reaction. J. Sci. Food Agric 1993; 62: 245–252
  • Lozano P., Cano J., Iborra J. L., Manjón A. Glycylglycylphenylalaninamide synthesis catalysed by papain in a medium containing polyols. Biotechnol. Appl. Biochem 1993; 18: 67–74
  • Lozano P., Combes D., Iborra J. L. Effect of polyois on α-chymotrypsin thermostability: a mechanistic analysis of the enzyme stabilizaton. J. Biotechnol 1994a; 35: 9–18
  • Lozano P., Iborra J. L., Rocha A. J. P. Cryo-enzymatic synthesis of kyothorphin in saline environments. Biochem. Molec. Biol. Intl 1994b; 33: 91–97
  • Melander W., Horvarth C. Salts effects on hydrophobic interaction in precipitation and chromatography of proteins: An interpretation of the lyotropic series. Arch. Biochem. Biophys 1977; 18: 200–215
  • Mozhaev V. V., Berezin I. V., Martinek K. Structure-stability relationship in proteins: Fundamental tasks and strategy for the development of stabilized enzyme catalysts for biotechnology. CRC Critical Rev. Biochem 1988; 23: 235–282
  • Przybycien T. M., Bailey J. E. Solubility-activity relationships in the inorganic salt-induced precipitation of α-chymotrypsin. Enzyme Microb. Technol 1989; 11: 264–276
  • Schmid R. D. Stabilized soluble enzymes. Adv. Biochem. Eng 1979; 12: 41–118
  • Shih Y. Ch., Prausnitz J. M., Blanch H. W. Some characteristic of protein precipitation by salts. Biotechnol. Bioeng 1992; 40: 1155–1164
  • Sigmaplot Scientific Graphic System. Jandel Scientific Co. 1992, Version 5.01
  • Stevenson D. E., Storer A. C. Papain in organic solvents: Determination of conditions suitable for biocatalysis and the effect on substrate specificity and inhibitions. Biotecnol. Bioeng 1991; 37: 519–527
  • von Hippel P. H., Peticolas V., Schack L., Karlson L., Hamabato A., Chang S. Model studies on the effects of neutral salts on the conformational stability of biological macromolecules. Biochemistry 1973; 12: 1256–1282
  • Wada A., Saito Y., Ohogushi M. Multiphasic conformaton transition of globular protein under denaturing conditions. Biopolymers 1983; 22: 93–99

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