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Original Article

The Tetrazolium Dyes MTS and XTT Provide New Quantitative Assays for Superoxide and Superoxide Dismutase

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Pages 283-289 | Received 01 Apr 1997, Published online: 07 Jul 2009

References

  • Beauchamp C., Fridovich I. Superoxide dismutase: improved assays and an assay Applicable to acrylamide gels. Analytical Biochemistry 1971; 44: 276–287
  • McCord J. M., Fridovich I. Superoxide dismutase. An enzymic function for erythrocuprein (hemo‐cuprein). Journal of Biological Chemistry 1969; 244: 6049–6055
  • Seidler E. The tetrazolium‐formazan system: design and histochemistry. Progress in Histochemistry and Cytochemistry 1991; 24: 1–86
  • Apostol I., Heinstein P. F., Low P. S. Rapid stimulation of an oxidative burst during elicitation of cultured plant cells. Plant Physiology 1989; 90: 109–116
  • Sutherland M. W. The generation of oxygen radicals during host plant responses to infection. Physiological and Molecular Plant Pathology 1991; 39: 79–93
  • Paull K. D., Shoemaker R. H., Boyd M. R., Parsons J. L., Risbood P. A., Barbera W. A., Sharma M. N., Baker D. C., Hand E., Scudiero D. A., Monks A., Alley M. C., Grote M. The synthesis of XTT: A new tetrazolium reagent that is bioreducible to a water‐soluble formazan. Journal of Heterocyclic Chemistry 1988; 25: 911–914
  • Scudiero J. D., Shoemaker R. H., Paull K. D., Monks A., Tiemey S., Nofziger T. H., Currens M. J., Seniff D., Boyd M. R. Evaluation of a soluble tetrazolium /formazan assay for cell growth and drug sensitivity in culture using human and other tumor cell lines. Cancer Research 1988; 48: 4827–4833
  • Barltrop J. A., Owen T. G., Cory A. H., Cory J. G. 5‐(3‐carboxymethoxyphenyl)‐2‐(4, 5‐dimethylthi‐azolyl)‐3‐(4‐sulfophenyl)tetrazolium, inner salt (MTS) and related analogs of 3‐(4, 5‐dimethylthiazolyl)‐2, 5‐diphenyltetrazolium bromide(MTT) reducing to purple water‐soluble formazans as cell‐viability indicators. Bioorganic & Medicinal Chemistry Letters 1991; 1: 611–614
  • Cory A. H., Owen T. C., Barltrop J. A., Cory J. G. Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture. Cancer Communications 1991; 3: 207–212
  • Goodwin C. J., Holt S. J., Downes S., Marshall N. J. Microculture tetrazolium assays: A comparison between two new tetrazolium salts, XTT and MTS. Journal of Immunological Methods 1995; 179: 95–103
  • Dunigan D. D., Waters S. B., Owen T. C. Aqueous soluble tetrazolium/formazan MTS as an indicator of NADH‐ and NADPH‐dependent dehydro‐genase activity. BioTechniques 1995; 19: 640–649
  • Goldstein S., Michel C., Bors W., Saran M., Czapski G. A critical reevaluation of some assay methods for superoxide dismutase activity. Free Radical Biology & Medicine 1988; 4: 295–303
  • Goldstein S., Czapski G. Comparison between different assays for superoxide dismutase‐like activity. Free Radical Research Communications 1991; 12–13; 5–10
  • Bielski B. H. J., Cabelli D. E., Arudi R. L., Ross A. B. Reactivity of HO2/O2‐‐ radicals in aqueous solution. Journal of Physical and Chemical Reference Data 1985; 14: 1041–1100
  • Massey V. The microestimation of succinate and the extinction coefficient of cytochrome c. Biophysica Biochimica Acta 1959; 34: 255–256
  • Spitz D. R., Oberley L. W. An assay for superoxide dismutase activity in mammalian tissue homogenates. Analytical Biochemistry 1989; 179: 8–18
  • Burdon R. H., Gill V., Rice‐Evans C. Reduction of a tetrazolium salt and superoxide generation in human tumor cells (HeLa). Free Radical Research Communications 1993; 18: 369–380
  • Hammond‐Kosack K. E., Jones J. D. G. Resistance gene‐dependent plant defense responses. The Plant Cell 1996; 8: 1773–1791
  • Azzi A., Montecucco C., Richter C. The use of acetylated ferricytochrome c for the detection of superoxide radicals produced in biological membranes. Biochemical and Biophysical Research Communications 1975; 65: 597–603

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