Characterization of Mamalian G_S-α Proteins Expressed in Yeast

References

• Stryer L., Bourne H. R. G Proteins: A family of signal transducers. Annu. Rev. Cell Biol. 1986; 2: 391–419
   PubMedGoogle Scholar
• Gilman A. G. G Proteins: Transducers of receptor-generated signals. Annu. Rev. Biochem. 1987; 56: 615–649
   PubMed Web of Science ®Google Scholar
• Deitzel C., Kurjan J. The yeast SCG1 gene: a Gα-like protein implicated in the a- and α-factor response pathway. Cell 1987; 50: 1001–1010
   PubMed Web of Science ®Google Scholar
• Miyajima I., Nakafuku M., Nakayama N., Brenner C., Miyajima A., Kaibuchi K., Arai K., Kaziro Y., Matsumoto K. GPA1, a haploid-specific essential gene, encodes a yeast homolog of mammalian G protein which may be involved in mating factor signal transduction. Cell 1987; 50: 1011–1019
   PubMed Web of Science ®Google Scholar
• Whiteway M., Hougan L., Dignard D., Thomas D. Y., Bell L., Saari G. C., Grant F. J., O'Hara P., MacKay V. L. The STE4 and STE18 genes of yeast encode potential β and γ subunits of the mating factor receptor-coupled G protein. Cell 1989; 56: 467–477
   PubMed Web of Science ®Google Scholar
• Kang Y.-S., Kane J., Kurjan J., Stadel J. M., Tipper D. J. Effects of expression of mammalian Gα and hybrid mammalian-yeast Gα proteins on the yeast pheromone response signal transduction pathway. Molec. Cell. Biol. 1990; 10: 2582–2590
   PubMed Web of Science ®Google Scholar
• Northup J. K., Sternweis P. C., Smigel M. D., Schleifer L. S., Ross E. M., Gilman A. G. Purification of the regulatory component of adenylate cyclase. Proc. Natl. Acad. Sci. USA 1980; 77: 6515–6520
   Web of Science ®Google Scholar
• Bokoch G. M., Katada T., Northup J. K., Ui M., Gilman A. G. Purification and properties of the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. J. Biol. Chem. 1984; 259: 3560–3567
   PubMed Web of Science ®Google Scholar
• Kuhn H. Light- and GTP-regulated interaction of GTPase and other proteins with bovine photoreceptor membranes. Nature (London) 1980; 283: 587–589
   PubMed Web of Science ®Google Scholar
• Codina J., Hildebrandt J. D., Sekura R. D., Birnbaumer M., Bryan J., Manclark R., Iyengar R., Birnbaumer L. NS and Ni the stimulatory and inhibitory regulatory components of adenylyl cyclase. J. Biol. Chem. 1984; 259: 5871–5886
   PubMed Web of Science ®Google Scholar
• Nukuda T., Tanabe T., Takahashi H., Noda M., Hirose T., Inayama S., Numa S. Primary structure of the α-subunit of bovine adenylate cyclase-stimulating G-protein deduced from the cDNA sequence. FEBS Lett. 1986; 195: 220–224
   PubMed Web of Science ®Google Scholar
• Robishaw J. D., Russell D. W., Harris B. A., Smigel M. D., Gilman A. G. Deduced primary structure of the α subunit of the GTP-binding stimulatory protein of adenylate cyclase. Proc. Natl. Acad. Sci. USA 1986; 83: 1251–1255
   PubMed Web of Science ®Google Scholar
• Itoh H., Kozasa T., Nagata S., Nakanimura S., Katada T., Ui M., Iwai S., Ohtsuka E., Kawasaki H., Suzuki K., Kaziro Y. Molecular cloning and sequence determination of cDNAs for α subunits of the guanine nucleotide-binding proteins GSGi and GO from rat brain. Proc. Natl. Acad. Sci. USA 1986; 83: 3776–3780
   PubMed Web of Science ®Google Scholar
• Bray P., Carter A., Simons C., Guo V., Puckett C., Kamholtz J., Speigel A., Nirenberg M. Human cDNA clones for four species of GαS signal transduction protein. Proc. Natl. Acad. Sci. USA 1986; 71: 8893–8897
   Google Scholar
• Robishaw J. D., Smigel M. D., Gilman A. G. Molecular basis for two forms of the G protein that stimulates adenylate cyclase. J. Biol. Chem. 1986; 261: 9587–9590
   PubMed Web of Science ®Google Scholar
• Kozasa T., Itoh H., Tsukamoto T., Kaziro Y. Isolation and characterization of the human GSα gene. Proc. Natl. Acad. Sci. USA 1988; 85: 2081–2085
   PubMed Web of Science ®Google Scholar
• Sternweis P. C., Northup J. K., Smigel M. D., Gilman A. G. The regulatory component of adenylate cyclase. J. Biol. Chem. 1981; 256: 11517–11526
   PubMed Web of Science ®Google Scholar
• Birnbaumer L., Codina J., Mattera R., Cerione R. A., Hildebrandt J. D., Sunyer T., Rojas F. J., Caron M. G., Lefkowitz R. J., Iyengar R. Regulation of hormone receptors and adenylyl cyclase by guanine nucleotide binding N proteins. Rec. Prog. Horm. Res. 1985; 41: 41–99
   PubMedGoogle Scholar
• Graziano M. P., Casey P. J., Gilman A. G. Expression of cDNAs for G proteins in Escherichia coli. J. Biol. Chem. 1987; 262: 11375–11381
   PubMed Web of Science ®Google Scholar
• Olate J., Mattera R., Codina J., Birnbaumer L. Reticulocyte lysates synthesize an active α subunit of the stimulatory G protein GS J. Biol. Chem. 1988; 263: 10394–10400
   Web of Science ®Google Scholar
• Stadel J. M., Johnson R. K., Mirabelli C. K., Powers D. A., Sung C.-M., Faucette L. F., McCabe F. L., Crooke S. T. Tumorgenicity of the cyc− variant of the S49 murine lymphoma deficient in the GS-α subunit of adenylate cyclase. Cancer Res. 1988; 48: 641–644
   PubMed Web of Science ®Google Scholar
• Jones D. T., Reed R. R. Molecular cloning of five GTP-binding protein cDNA species from rat olfactory neuroepithelium. J. Biol. Chem. 1987; 262: 14241–14249
   PubMed Web of Science ®Google Scholar
• Ecker D. J., Kahn M. I., Marsh J., Butt T. R., Crooke S. T. Chemical synthesis and expression of a cassette adapted ubiquitin gene. J. Biol. Chem. 1987; 262: 3524–3531
   PubMed Web of Science ®Google Scholar
• Ecker D. J., Butt T. R., Sternberg E. J., Neeper M. P., Debouck C., Gorman J. A., Crooke S. T. Yeast metallothionein function in metal ion detoxification. J. Biol. Chem. 1986; 261: 16895–16900
   PubMed Web of Science ®Google Scholar
• Young J. F., Desselberger U., Palese P., Ferguson B., Shatzman A. R., Rosenberg M. Efficient expression of influenza virus NS1 nonstructural proteins in Escherichia coli. Proc. Natl. Acad. Sci. USA 1983; 80: 6105–6109
   PubMed Web of Science ®Google Scholar
• Gross M., Sweet R. W., Sathe G., Yokoyama S., Fasano O., Goldfarb M., Wigler M., Rosenberg M. Purification and characterization of human H-ras proteins expressed in Escherichia coli. Molec. Cell. Biol. 1985; 5: 1015–1024
   PubMed Web of Science ®Google Scholar
• Rosenberg M., Ho Y., Shatzman A. The use of pKC-30 and its derivatives for controlled expression of genes. Methods Enzymol. 1983; 101: 123–138
   PubMed Web of Science ®Google Scholar
• Laemmli U. K. Cleavage of structural proteins during the assembly of the heads of bacteriophage T4. Nature (London) 1970; 227: 680–685
   PubMed Web of Science ®Google Scholar
• Towbin H., Staehelin T., Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci, USA 1979; 76: 4350–4353
   PubMed Web of Science ®Google Scholar
• Mumby S. M., Kahn R. A., Manning D. R., Gilman A. G. Antisera of designed specificity for subunits of guanine nucleotide-binding regulatory proteins. Proc. Natl. Acad. Sci. USA 1986; 83: 265–269
   PubMed Web of Science ®Google Scholar
• Ross E. M., Howlett A. C., Ferguson K. M., Gilman A. G. Reconstitution of hormone-sensitive adenylate cyclase activity with resolved components of the enzyme. J. Biol. Chem. 1978; 253: 6406–6412
   Web of Science ®Google Scholar
• Iyengar R., Bhat M. K., Riser M. E., Birnbaumer L. Receptor-specific desensitization of the S49 lymphoma cell adenylyl cyclase. J. Biol. Chem. 1981; 256: 4810–4815
   PubMed Web of Science ®Google Scholar
• Briggs M. M., Stadel J. M., Iyengar R., Lefkowitz R. J. Functional modification of the guanine nucleotide regulatory protein after desensitization of turkey erythrocytes by catecholamines. Arch. Biochem. Biophys. 1983; 224: 141–151
   Web of Science ®Google Scholar
• Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provensano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985; 150: 76–85
   PubMed Web of Science ®Google Scholar
• Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951; 193: 265–275
   PubMed Web of Science ®Google Scholar
• Graziano M. P., Freissmuth M., Gilman A. G. Expression of Gsα in Escherichia coli. J. Biol. Chem. 1989; 264: 409–418
   PubMed Web of Science ®Google Scholar
• Williams D. C., Frank R. M., Muth W. L., Burnett J. P. Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins. Science 1983; 215: 687–689
   Web of Science ®Google Scholar
• Sternweis P. C. The purified α subunits of GO and Gi from bovine brain require βγ for association with phospholipid vesicles. J. Biol. Chem. 1986; 261: 631–637
   PubMed Web of Science ®Google Scholar
• Linder M. E., Middleton P., Helper J. R., Taussig R., Gilman A. G., Mumby S. M. Lipid modification of G proteins: α subunits are palmitoylated. Proc. Natl. Acad. Sci. USA 1993; 90: 3675–3679
   PubMed Web of Science ®Google Scholar
• Buss J. E., Mumby S. M., Casey P. J., Gilman A. G. Myristoylated α subunits of guanine nucleotide-binding regulatory proteins. Proc. Natl. Acad. Sci. USA 1987; 84: 7493–7497
   PubMed Web of Science ®Google Scholar
• Hekman M., Holzhofoer A., Gierschik P., Im M.-J., Jakobs K.-H., Pfeuffer T., Helmreich J. M. Regulation of signal transfer from β1-adrenoceptor to adenylate cyclase by βγ subunits in a reconstituted system. Eur. J. Biochem. 1987; 169: 431–439
   PubMedGoogle Scholar
• Cerione R. A., Staniszewski R., Gierschik P., Codina J., Somers R. L., Birnbaumer L., Speigel A. M., Caron M. G., Lefkowitz R. J. Mechanism of guanine nucleotide regulatory protein-mediated inhibition of adenylate cyclase. J. Biol. Chem. 1986; 261: 9514–9520
   PubMed Web of Science ®Google Scholar