The aspHS gene as a new target for detecting Aspergillus fumigatus during infections by quantitative real-time PCR

References

• Summerbell R. Pathogenic fungi in humans and animals. In: Howard DH (ed). Ascomycetes: Aspergillus, Fusarium, Sporothrix, Piedraia and their Relatives. 2nd edn. New York: Marcel Decker, 2003: 237–498.
   Google Scholar
• Abad A, Fernandez-Molina JV, Bikandi J, et al. What makes Aspergillus fumigatus a successful pathogen? Genes and molecules involved in invasive aspergillosis. Rev Iberoam Micol 2010; 27: 155–182.
   PubMed Web of Science ®Google Scholar
• del Palacio A, Cuetara MS, Ponton J. Invasive aspergillosis. Rev Iberoam Micol 2003; 20: 77–78.
   Google Scholar
• Quindós G, Eraso E. Importancia actual de la aspergilosis invasora [Current importance of invasive aspergillosis]. In: Pontón J (ed). Aspergilosis Invasora [Invasive Aspergillosis]. 2nd edn. Bilbao, Spain: Revista Iberoamericana de Micología, 2008: 3–6.
   Google Scholar
• De Pauw B, Walsh TJ, Donnelly JP, et al. Revised definitions of invasive fungal disease from the European Organization for Research and Treatment of Cancer/Invasive Fungal Infections Cooperative Group and the National Institute of Allergy and Infectious Diseases Mycoses Study Group (EORTC/MSG) Consensus Group. Clin Infect Dis 2008; 46: 1813–1821.
   PubMed Web of Science ®Google Scholar
• del Palacio A, Cuétara MS, Alhambra A. Detecci n de ant genos [Antigen detection] (ELISA: Platelia Aspergillus). In: Pontón J (ed). Aspergilosis Invasora [Invasive Aspergillosis]. 2nd edn. Bilbao, Spain: Revista Iberoamericana de Micología, 2008: 87–101.
   Google Scholar
• Pazos C, Pontón J, del Palacio A. Contribution of (1,3)-beta-D-glucan chromogenic assay to diagnosis and therapeutic monitoring of invasive aspergillosis in neutropenic adult patients: a comparison with serial screening for circulating galactomannan. J Clin Microbiol 2005; 43: 299–305.
   PubMed Web of Science ®Google Scholar
• Barnes PD, Marr KA. Risks, diagnosis and outcomes of invasive fungal infections in haematopoietic stem cell transplant recipients. Br J Haematol 2007; 139: 519–531.
   PubMed Web of Science ®Google Scholar
• Hachem RY, Kontoyiannis DP, Chemaly RF, Jiang Y, Reitzel R, Raad I. Utility of galactomannan enzyme immunoassay and (1,3) beta- D-glucan in diagnosis of invasive fungal infections: low sensitivity for Aspergillus fumigatus infection in hematologic malignancy patients. J Clin Microbiol 2009; 47: 129–133.
   PubMed Web of Science ®Google Scholar
• White PL, Mengoli C, Bretagne S, et al. Evaluation of Aspergillus PCR protocols for testing serum specimens. J Clin Microbiol 2011; 49: 3842–3848.
   PubMed Web of Science ®Google Scholar
• Rementeria A, Lopez-Molina N, Ludwig A, et al. Genes and molecules involved in Aspergillus fumigatus virulence. Rev Iberoam Micol 2005; 22: 1–23.
   PubMedGoogle Scholar
• Abad-Diaz-de-Cerio A. Development of molecular techniques based on PCR for detection of Aspergillus fumigatus [Desarrollo de técnicas moleculares basadas en la PCR para la detección de Aspergillus fumigatus] , PhD thesis. Universidad del Pa s Vasco [University of the Basque Country (UPV/EHU)], Leioa, Spain, 2009.
   Google Scholar
• Hummel M, Baust C, Kretschmar M, et al. Detection of Aspergillus DNA by a nested PCR assay is superior to blood culture in an experimental murine model of invasive aspergillosis. J Med Microbiol 2004; 53: 803–806.
   PubMed Web of Science ®Google Scholar
• Liu D, Coloe S, Baird R, Pederson J. Rapid mini-preparation of fungal DNA for PCR. J Clin Microbiol 2000; 38: 471.
   PubMed Web of Science ®Google Scholar
• Hoorfar J, Ahrens P, Radstrom P. Automated 5′ nuclease PCR assay for identification of Salmonella enterica. J Clin Microbiol 2000; 38: 3429–3435.
   PubMed Web of Science ®Google Scholar
• Dennis Lo YM, Allen Chan KC. Setting up a polymerase chain reaction laboratory. In: Dennis Lo YM, Chiu RWK, Allen Chan KC (eds). Clinical Applications of PCR. 2nd edn. New Jersey: Humana Press, 2006: 11–18.
   Google Scholar
• Bustin SA, Benes V, Garson JA, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009; 55: 611–622.
   PubMed Web of Science ®Google Scholar
• Barnes PD, Marr KA. Risks, diagnosis and outcomes of invasive fungal infections in haematopoietic stem cell transplant recipients. Br J Haematol 2007; 139: 519–531.
   PubMed Web of Science ®Google Scholar
• Chamilos G, Luna M, Lewis RE, et al. Invasive fungal infections in patients with hematologic malignancies in a tertiary care cancer center: an autopsy study over a 15-year period (1989–2003). Haematologica 2006; 91: 986–989.
   PubMed Web of Science ®Google Scholar
• Costa C, Costa JM, Desterke C, et al. Real-time PCR coupled with automated DNA extraction and detection of galactomannan antigen in serum by enzyme-linked immunosorbent assay for diagnosis of invasive aspergillosis. J Clin Microbiol 2002; 40: 2224–2227.
   PubMed Web of Science ®Google Scholar
• Sanguinetti M, Posteraro B, Pagano L, et al. Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis. J Clin Microbiol 2003; 41: 3922–3925.
   PubMed Web of Science ®Google Scholar
• Challier S, Boyer S, Abachin E, Berche P. Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis. J Clin Microbiol 2004; 42: 844–846.
   PubMed Web of Science ®Google Scholar
• Yokota K, Shimada H, Kamaguchi A, Sakaguchi O. Studies on the toxin of Aspergillus fumigatus. VII. Purification and some properities of hemolytic toxin (asp-hemolysin) from culture filtrates and mycelia. Microbiol Immunol 1977; 21: 11–22.
   PubMed Web of Science ®Google Scholar
• Ebina K, Sakagami H, Yokota K, Kondo H. Cloning and nucleotide sequence of cDNA encoding Asp-hemolysin from Aspergillus fumigatus. Biochim Biophys Acta 1994; 1219: 148–150.
   PubMed Web of Science ®Google Scholar
• Kumagai T, Nagata T, Kudo Y, Fukuchi Y, Ebina K, Yokota K. Cytotoxic activity and cytokine gene induction of Asp-hemolysin to murine macrophages. Nihon Ishinkin Gakkai Zasshi 1999; 40: 217–222.
   Google Scholar
• Gravelat FN, Doedt T, Chiang LY, et al. In vivo analysis of Aspergillus fumigatus developmental gene expression determined by real-time reverse transcription-PCR. Infect Immun 2008; 76: 3632–3639.
   PubMed Web of Science ®Google Scholar
• Wartenberg D, Lapp K, Jacobsen ID, et al. Secretome analysis of Aspergillus fumigatus reveals Asp-hemolysin as a major secreted protein. Int J Med Microbiol 2011; 301: 602–611.
   PubMed Web of Science ®Google Scholar
• Khot PD, Ko DL, Hackman RC, Fredricks DN. Development and optimization of quantitative PCR for the diagnosis of invasive aspergillosis with bronchoalveolar lavage fluid. BMC Infect Dis 2008; 8: 73.
   PubMed Web of Science ®Google Scholar
• Balajee SA, Gribskov JL, Hanley E, Nickle D, Marr KA. Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus. Eukaryot Cell 2005; 4: 625–632.
   PubMedGoogle Scholar
• Balajee SA, Nickle D, Varga J, Marr KA. Molecular studies reveal frequent misidentification of Aspergillus fumigatus by morphotyping. Eukaryot Cell 2006; 5: 1705–1712.
   PubMedGoogle Scholar
• Alcazar-Fuoli L, Mellado E, Alastruey-Izquierdo A, Cuenca-Estrella M, Rodriguez-Tudela JL. Aspergillus section Fumigati: antifungal susceptibility patterns and sequence-based identification. Antimicrob Agents Chemother 2008; 52: 1244–1251.
   PubMed Web of Science ®Google Scholar
• Vinh DC, Shea YR, Sugui JA, et al. Invasive aspergillosis due to Neosartorya udagawae. Clin Infect Dis 2009; 49: 102–111.
   PubMed Web of Science ®Google Scholar
• Cenci E, Perito S, Enssle KH, et al. Th1 and Th2 cytokines in mice with invasive aspergillosis. Infect Immun 1997; 65: 564–570.
   PubMed Web of Science ®Google Scholar
• Latge JP. Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 1999; 12: 310–350.
   PubMed Web of Science ®Google Scholar
• Tuon FF. A systematic literature review on the diagnosis of invasive aspergillosis using polymerase chain reaction (PCR) from bronchoalveolar lavage clinical samples. Rev Iberoam Micol 2007; 24: 89–94.
   PubMedGoogle Scholar
• Paterson DL, Singh N. Invasive aspergillosis in transplant recipients. Medicine (Baltimore) 1999; 78: 123–138.
   PubMed Web of Science ®Google Scholar
• Abu Al-Soud W, Radstrom P. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J Clin Microbiol 2000; 38: 4463–4470.
   PubMed Web of Science ®Google Scholar