Human mesenchymal stromal cells preserve their stem features better when cultured in the Dulbecco's modified Eagle medium

References

• Prockop DJ. Marrow stroma cells as stem cells for non-hematopoietic tissues. Science. 1997;276:71–4.
   PubMed Web of Science ®Google Scholar
• Pittenger MF, MacKay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, . Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284:143–7.
   PubMed Web of Science ®Google Scholar
• De Bari C, Dell'Accio F, Vandenabeele F, Veermesch JR, Raymackers JM, Luyten FP. Skeletal muscle repair by adult human mesenchymal stem cells from synovial membrane. J Cell Biol. 2003;160:909–18.
   PubMed Web of Science ®Google Scholar
• Akijama Y, Radtke C, Honmou O, Kocsis JD. Remyelination of the spinal cord following intravenous delivery of bone marrow cells. Glia. 2002;39:229–36.
   PubMedGoogle Scholar
• Sato Y, Araki H, Kato J, Nakamura K, Kawano Y, Kobune M, . Human mesenchymal stem cells xenografted directly to rat liver are differentiated into human hepatocytes without fusion. Blood. 2005;106:756–63.
   PubMed Web of Science ®Google Scholar
• Woodbury D, Schwarz EJ, Prockop DJ, Black IB. Adult rat and human bone marrow stromal cells differentiate into neurons. Neurosci Res. 2000;61:364–70.
   Web of Science ®Google Scholar
• Barry FP. Biology and clinical applications of mesenchymal stem cells. Birth Defects Res C Embryo Today. 2003;48: 3464–74.
   Google Scholar
• Bartholomew A, Sturgeon C, Siatskas M, Ferrer K, McIntosh K, Patil S, . Mesenchymal stem cells suppress lymphocyte proliferation in vitro and prolong skin graft survival in vivo. Exp Hematol. 2002;31:42–8.
   Google Scholar
• Le Blanc K, Frassoni F, Ball L, Locatelli F, Roelofs H, Lewis I, . Developmental Committee of the European Group for Blood and Marrow Transplantation, mesenchymal stem cells for treatment of steroid-resistant, severe, acute graft-versus-host-disease: a phase II study. Lancet. 2008;371:1579–86.
   PubMed Web of Science ®Google Scholar
• Sbano P, Cuccia A, Mazzanti B, Urbani S, Giusti B, Lapini I, . Use of donor bone marrow mesenchymal stem cells for treatment of skin allograft rejection in a preclinical rat model. Arch Dermatol Res. 2008;300:115–24.
   PubMedGoogle Scholar
• Urbani S, Caporale R, Lombardini L, Bosi A, Saccardi R. Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells. Cytotherapy. 2006;8: 243–53.
   PubMed Web of Science ®Google Scholar
• Colter DC, Sekiya I, Prockop DJ. Identification of a subpopulation of rapidly self-renewing and multipotential adult stem cells in colonies of human marrow stromal cells. Proc Natl Acad Sci USA. 2001;98:7841–5.
   PubMed Web of Science ®Google Scholar
• Lee RH, Hsu SC, Munoz J, Jung JS, Lee NR, Pochampally R, Prockop DJ. A subset of human rapidly self renewing marrow stromal cells preferentially engraft in mice. Blood. 2006;107:2153–61.
   PubMed Web of Science ®Google Scholar
• Colter DC, Class R, Di Girolamo CM, Prockop DJ. Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow. Cell Biology. 2000;97: 3213–8.
   Google Scholar
• Lee KA, Wooyoung S, Paik MJ, Lee SC, Shim JY, Ahn YH, . Analysis of changes in the viability and gene expression profiles of human mesenchymal stromal cells over time. Cytotherapy. 2009;11:688–97.
   PubMed Web of Science ®Google Scholar
• Sorensen MH, Friis T, Bindslev L, Mortensen S, Johnsen HE, Kastrup J. Comparison of different culture conditions for human mesenchymal stromal cells for clinical stem cell therapy. Scand J Clin Lab Invest. 2008;68:192–203.
   PubMedGoogle Scholar
• Wagner W, Ho AD. Mesenchymal stem cells preparations comparing apples and oranges. Stem Cell Rev. 2007;3: 239–48.
   PubMed Web of Science ®Google Scholar
• Wagner W, Feldmann RE Jr, Seckinger A, Maurer MH, Wein F, Krause U, . The heterogeneity of human mesenchymal stem cells preparations: evidence from simultaneous analysis of proteomes and transcriptomes. Exp Hematol. 2006;34:536–48.
   PubMed Web of Science ®Google Scholar
• Wenisch S, Trinkaus K, Hild A, Hose D, Heiss C, Alt V, . Immunochemical, ultrastructural and electrophysiological investigations of bone-derived stem cells in the course of neuronal differentiation. Bone. 2006;38:911–21.
   PubMedGoogle Scholar
• Titorencu I, Jinga VV, Constantinescu E, Gafencu AV, Ciohodaru C, Manolescu I, . Proliferation, differentiation and characterization of osteoblasts from human BM mesenchymal cells. Cytotherapy. 2007;9:682–96.
   PubMed Web of Science ®Google Scholar
• Jazayeri M, Allameh A, Soleimani M, Jazayeri SH, Piryaei A, Kazemnejad S. Molecular and ultrastructural characterization of endothelial cells differentiated from human bone marrow mesenchymal stem cells. Cell Biol Int. 2008;32:1183–92.
   PubMed Web of Science ®Google Scholar
• Pasquinelli G, Tazzari P, Ricci F, Vaselli C, Buzzi M, Conte R, . Ultrastructural characteristics of human mesenchymal stromal (stem) cells derived from bone marrow and term placenta. Ultrastruct Pathos. 2007;31:23–8.
   PubMed Web of Science ®Google Scholar
• Castro-Malaspina H, Gay RE, Resnick G, Kapoor N, Meyers P, Chiarieri D, . Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny. Blood. 1980;56:289–301.
   PubMed Web of Science ®Google Scholar
• Friedenstein AJ, Gorskaja JF, Kulagina NN. Fibroblast precursors in normal and irradiated mouse hematopoietic organs. Exp Hematol. 1976;4:267–74.
   PubMed Web of Science ®Google Scholar
• Mazzanti B, Aldinucci A, Biagioli T, Barilaro A, Urbani S, Dal Pozzo S, . Differences in mesenchymal stem cell cytokine profiles between MS patients and healthy donors: implication for assessment of disease activity and treatment. J Neuroimmunol. 2008;199:142–50.
   PubMed Web of Science ®Google Scholar
• Wuchter P, Boda-Heggemann J, Straub BK, Grund C, Kuhn C, Krause U, . Processus and recessus adhaerentes: giant adherens cell junction systems connect and attract human mesenchymal stem cells. Cell Tissue Res. 2007;328: 499–514.
   PubMed Web of Science ®Google Scholar
• Franke WW, Grund C, Jackson BW, Illmensee K. Formation of cytoskeletal elements during mouse embryogenesis. IV. Ultrastructure of primary mesenchymal cells and their cell–cell interactions. Differentiation. 1983;25:121–41.
   PubMedGoogle Scholar
• Li YM, Benisch TP, Zeck S, Meissner-Weigl J, Schneider D, Limbert C, . Effects of high glucose on mesenchymal stem cell proliferation and differentiation. Biochem Biophys Res Commun. 2007;363:209–15.
   PubMedGoogle Scholar
• Stolzing A, Coleman N, Scutt A. Glucose-induced replicative senescence in mesenchymal stem cells, Rejuvenation Res. 2006;9:31–5.
   PubMedGoogle Scholar
• Sotiropoulou PA, Perez SA, Salagianni M, Baxevanis CN, Papamichail M. Characterization of the optimal culture conditions for clinical scale production of human mesenchymal stem cells. Stem Cells. 2006;24:462–71.
   PubMed Web of Science ®Google Scholar