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Research Article

Xylarinase: a novel clot busting enzyme from an endophytic fungus Xylaria curta

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Pages 1502-1511 | Received 16 Oct 2015, Accepted 28 Jan 2016, Published online: 31 Mar 2016

References

  • Mander P, Cho SS, Simkhada JR, et al. A low molecular weight chymotrypsin-like novel fibrinolytic enzyme from Streptomyces sp. CS624. Process Biochem 2011;46:1449–55
  • Mensah GA, Morany AE, Rothz GA, Narulah J. The global burden of ischemic heart disease in 1990 and 2010: the Global Burden of Disease 2010 study. Circulation 2014;9:183–4
  • Shirasaka N, Naitou M, Okamura K, et al. Purification and characterization of a fibrinolytic protease from Aspergillus oryzae KSK-3. Mycoscience 2012;53:354–64
  • White HD, Van de Werf FJJ. Thrombolysis for acute myocardial infarction. Circulation 1998;97:1632–46
  • Thames MD, Sease DR, Damian A. Ischemic heart diseases: an overview. Adv Stud Med 2004;4:794–802
  • Lu F, Sun L, Lu Z, et al. Isolation and identification of an endophytic strain EJS-3 producing novel fibrinolytic enzymes. Curr Microbiol 2007;54:435–9
  • Ellis K, Brener S. New fibrinolytic agents for MI: as effective as current agents, but easier to administer. Cleve Clin J Med 2004;71:20–37
  • Mackman N. Triggers, targets and treatments for thrombosis. Nature 2008;451:914–18
  • Choi BS, Sapkota K, Choi JH, et al. Herinase: a novel bi-functional fibrinolytic protease from the monkey head mushroom, Hericium erinaceum. Appl Biochem Biotechnol 2013;170:609–22
  • Kim HC, Choi BS, Sapkota K, et al. Purification and characterization of a novel, highly potent fibrinolytic enzyme from Paecilomyces tenuipes. Process Biochem 2011;46:1545–53
  • Kotb E. Activity assessment of microbial fibrinolytic enzymes. Appl Microbiol Biotechnol 2013;97:6647–65
  • Babu V, Devi CS. In vitro thrombolytic activity of purified streptokinase extracted from Streptococcus equinus VIT_VB2 isolated from bovine milk. J Thromb Thrombolysis 2015;39:71–8
  • Yadav S, Datt M, Singh S, Sahani S. Role of the 88–97 loop in plasminogen activation by streptokinase probed through site-specific mutagenesis. Biochim Biophys Acta 2008;1784:1310–18
  • Peng Y, Yang X, Zhang Y. Microbial fibrinolytic enzymes: an overview of source, production, properties, and thrombolytic activity in vivo. Appl Microbiol Biotechnol 2005;69:126–32
  • Rovati JI, Delgado OD, Figueroa LIC, Farina JI. A novel source of fibrinolytic activity: Bionectria sp., an unconventional enzyme-producing fungus isolated from Las Yungas rainforest (Tucumán, Argentina). World J Microb Biot 2010;26:55–62
  • Bacon CW, White JF. Microbial endophytes. New York: Marcel Dekker; 2000
  • Rodriguez RJ, White JF, Arnold AE, Redman RS. Fungal endophytes: diversity and functional roles. New Phytol 2009;182:314–30
  • White JFJ, Torres MS. Is plant endophyte-mediated defensive mutualism the result of oxidative stress protection? Physiol Plant 2010;138:440–6
  • White J, Bacon CW. The secret world of endophytes in perspective. Fungal Ecol 2012;5:287–8
  • Li Y, Shuang JL, Yuan WW, et al. Verticase: a fibrinolytic enzyme produced by Verticillium sp. Tj33, an endophyte of Trachelospermum jasminoides. J Integr Plant Biol 2007;49:1548–54
  • Ueda M, Kubo T, Miyatake K, Nakamura T. Purification and characterization of fibrinolytic alkaline protease from Fusarium sp. BLB. Appl Microbiol Biotechnol 2007;74:331–8
  • Wu B, Wu L, Chen D, et al. Purification and characterization of a novel fibrinolytic protease from Fusarium sp. CPCC 480097. J Ind Microbiol Biotechnol 2009;36:451–9
  • Schulz B, Wanke U, Draeger S, Aust HJ. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol Res 1993;97:1447–50
  • Ali UF, Ibrahim ZM. Production and some properties of fibrinolytic enzyme from Rhizomucor miehei (Cooney & Emerson) Schipper. J Appl Sci Res 2008;4:892–9
  • Bi Q, Chu J, Feng Y, et al. Purification and characterization of a new serine protease with fibrinolytic activity from the marine invertebrate, Urechis unicinctus. Appl Biochem Biotechnol 2013;170:525–40
  • Cui L, Dong MS, Chen XH, et al. A novel fibrinolytic enzyme from Cordyceps militaris, a Chinese traditional medicinal mushroom. World J Microb Biot 2008;24:483–9
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein–dye binding. Anal Biochem 1976;72:248–54
  • Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond) 1970;227:680–5
  • Blum H, Beier H, Gross HJ. Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis 1987;8:93–9
  • Bi Q, Han B, Liu W, et al. UFEIII, a fibrinolytic protease from the marine invertebrate, Urechis unicinctus. Biotechnol Lett 2013;35:1115–20
  • Kim SH, Choi NS, Lee WY. Fibrin zymography: a direct analysis of fibrinolytic enzymes on gels. Anal Biochem 1998;263:115–16
  • Kang SR, Choi JH, Kim DW, et al. A bifunctional protease from green alga Ulva pertusa with anticoagulant properties: partial purification and characterization. J Appl Phycol 2016;28:599--607
  • Astrup T, Mullertz S. The fibrin plate method for estimating fibrinolytic activity. Arch Biochem Biophys 1952;40:346–51
  • Matsubara K, Hori K, Matsuura Y, Miyazawa K. Purification and characterization of a fibrinolytic enzyme and identification of fibrinogen clotting enzyme in a marine green alga, Codium divaricatum. Comp Biochem Physiol B, Biochem Mol Biol 2000;125:137–43
  • Ju X, Cao X, Sun Y, et al. Purification and characterization of a fibrinolytic enzyme from Streptomyces sp. XZNUM 00004. World J Microbiol Biotechnol 2012;28:2479–86
  • Wu B, Wu L, Ruan L, et al. Screening of endophytic fungi with antithrombotic activity and identification of a bioactive metabolite from the endophytic fungal strain CPCC 480097. Curr Microbiol 2009;58:522–7
  • Mukherjee AK, Rai SK, Thakur R, et al. Bafibrinase: a non-toxic, non-hemorrhagic, direct-acting fibrinolytic serine protease from Bacillus sp. strain AS-S20-I exhibits in vivo anticoagulant activity and thrombolytic potency. Biochimie 2012;94:1300–8
  • White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: White BA, ed. PCR protocols: a guide to methods and applications. New York (NY): Academic Press; 1990;315–22
  • Meshram V, Kapoor N, Saxena S. Muscodor kashayum sp. nov. – a new volatile anti-microbial producing endophytic fungus. Mycology 2013;4:196–204
  • Tamura K, Peterson D, Peterson N, et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011;28:2731–9
  • Marder VJ, Novokhatny V. Direct fibrinolytic agents: biochemical attributes, preclinical foundation and clinical potential. J Thromb Haemost 2010;8:433–44
  • Cha WS, Park SS, Kim SJ, Choi D. Biochemical and enzymatic properties of a fibrinolytic enzyme from Pleurotus eryngii cultivated under solid-state conditions using corn cob. Bioresour Technol 2010;101:6475–81
  • Kim SB, Lee DW, Cheigh CI, et al. Purification and characterization of a fibrinolytic subtilisin-like protease of Bacillus subtilis TP-6 from an Indonesian fermented soybean, Tempeh. J Ind Microbiol Biotechnol 2006;33:436–44
  • Wang CT, Ji BP, Li B, et al. Purification and characterization of a fibrinolytic enzyme of Bacillus subtilis DC33, isolated from Chinese traditional Douchi. J Ind Microbiol Biotechnol 2006;33:750–8
  • Simkhada JR, Cho SS, Mander P, et al. Purification, biochemical properties and antithrombotic effect of a novel Streptomyces enzyme on carrageenan-induced mice tail thrombosis model. Thromb Res 2012;129:176–82
  • Hatsuzawa K, Nagahama M, Takahashi S, et al. Purification and characterization of furin, a Kex2-like processing endoprotease, produced in Chinese hamster ovary cells. J Biol Chem 1992;267:16094–9
  • Choi JH, Sapkota K, Park SE, et al. Thrombolytic, anticoagulant and antiplatelet activities of codiase, a bi-functional fibrinolytic enzyme from Codium fragile. Biochimie 2013;95:1266–77

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