3,137
Views
14
CrossRef citations to date
0
Altmetric
Research Article

Simple ITC method for activity and inhibition studies on human salivary α-amylase

, , , &
Pages 1648-1653 | Received 09 Dec 2015, Accepted 29 Feb 2016, Published online: 06 Apr 2016

References

  • Freyer MW, Lewis EA. Isothermal titration calorimetry: experimental design, data analysis, and probing macromolecule/ligand binding and kinetic interactions. Methods Cell Biol 2008;84:79–113
  • Saboury AA, Divsalar A, Ataie G, et al Inhibition study of adenosine deaminase by caffeine using spectroscopy and isothermal titration calorimetry. Acta Biochim Pol 2003;50:849–55
  • Perspicace S, Rufer AC, Thoma R, et al Isothermal titration calorimetry with micelles: thermodynamics of inhibitor binding to carnitine palmitoyltransferase 2 membrane protein. FEBS Open Bio 2013;3:204–11
  • Dahot MU, Saboury AA, Moosavi-Movahedi AA. Inhibition of β-amylase activity by calcium, magnesium and zinc ions determined by spectrophotometry and isothermal titration calorimetry. Inhib Med Chem 2004;19:157–60
  • D’Amico S, Sohier JS, Feller G. Kinetics and energetics of ligand binding determined by microcalorimetry: insights into active site mobility in a psychrophilic α-amylase. J Mol Biol 2006;385:1296–304
  • Karim N, Okada H, Kidokoro S. Calorimetric evaluation of the activity and the mechanism of cellulases for the hydrolysis of cello-oligosaccharides accompanied by the mutarotation reaction of the hydrolyzed products. Thermochim Acta 2004;412:91–6
  • Baumann MJ, Murphy L, Lei N, et al Advantages of isothermal titration calorimetry for xylanase kinetics in comparison to chemical-reducing-end assays. Anal Biochem 2011;410:19–26
  • Todd MJ, Gomez J. Enzyme kinetics determined using calorimetry: a general assay for enzyme activity? Anal Biochem 2001;296:179–87
  • Draczkowski P, Tomaszuk A, Halczuk P, et al Determination of affinity and efficacy of acetylcholinesterase inhibitors using isothermal titration calorimetry. BBA Gen Subjects 2015;1860:967–74
  • Maximova K, Trylska J. Kinetics of trypsin catalyzed hydrolysis determined by isothermal titration calorimetry. Anal Biochem 2015;486:24–34
  • Saboury AA, Divsalar A, Jafari GA, et al A product inhibition study on adenosine deaminase by spectroscopy and calorimetry. J Biochem Mol Biol 2003;35:302–5
  • Jeoh T, Baker JO, Ali MK, et al β-d-Glucosidase reaction kinetics from isothermal titration microcalorimetry. Anal Biochem 2005;347:244–53
  • Kandra L. α-Amylases of medical and industrial importance. J Mol Struct 2003;666–667:487–98
  • Ramasubbu N, Ragunath C, Sundar K, et al Structure-function relationships in human salivary α-amylase: role of aromatic residues. Biologia 2005;16:47–56
  • Pickup JC, William G. Textbook of diabetes. 3rd ed. Vol. 2. Oxford: Blackwell Science Ltd.; 2003:73.1–.21
  • Kaufman RA, Tietz NW. Recent advances in measurement of amylase activity-a comparative study. Clin Chem 1980;26:846–53
  • Zhizhuang X, Storms R, Tsang A. A quantitative starch–iodine method for measuring alpha-amylase and glucoamylase activities. Anal Biochem 2006;351:146–8
  • Manitchotpisi P, Skory CD, Leathers TD, et al α-Amylase activity during pullulan production and α-amylase gene analyses of Aureobasidium pullulans. J Ind Microbiol Biotechnol 2011;38:1211–18
  • Tran PL, Lee JS, Park KH. Experimental evidence for a 9-binding subsite of Bacillus licheniformis thermostable α-amylase. FEBS Lett 2014;588:620–4
  • Mótyán JA, Fazekas E, Mori H, et al Transglycosylation by barley α-amylase. J Mol Catal B Enzym 2011;72:229–37
  • Xu Y, Zhang Z, Li L, et al Catechins play key role in green tea extract-induced postprandial hypoglycemic potential in vitro. Eur Food Res Technol 2013;237:89–99
  • Lorentz K, Gütschow B, Renner F. Evaluation of a direct α-amylase assay using 2-chloro-4-nitrophenyl-α-D-maltotrioside. Clin Chem Lab Med 2005;37:1053–62
  • Manaharan T, Appleton D, Cheng HM, Palanisamy UD. Flavonoids isolated from Syzygium aqueum leaf extract as potential antihyperglycaemic agents. Food Chem 2012;132:1802–7
  • Kumar PS, Sudha S. Evaluation of alpha-amylase and alpha-glucosidase inhibitory properties of selected seaweeds from Gulf of Mannar. Int Res J Pharm 2012;3:128–30
  • Kellogg J, Grace MH, Lila MA. Phlorotannins from Alaskan seaweed inhibit carbolytic enzyme activity. Marine Drugs 2014;12:5277–94
  • Lorentz K. Routine alpha-amylase assay using protected 4-nitrophenyl-1, 4-alpha-D-maltoheptaoside and a novel alpha-glucosidase. Clin Chem 2000;46:644–9
  • Morishita Y, Iinuma Y, Nakashima N, et al Total and pancreatic amylase measured with 2-chloro-4-nitrophenyl-4-O-beta-D-galactopyranosylmaltoside. Clin Chem 2000;46:928–33
  • Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248–54
  • Farkas E, Jánossy L, Harangi J, et al Synthesis of chromogenic substrates of alpha-amylases on a cyclodextrin basis. Carbohydr Res 1997;303:407–15
  • Winn-Deen ES, David H, Sigler G, Chavez R. Development of a direct assay for alpha-amylase. Clin Chem 1988;34:2005–8
  • Transtrum MK, Hansen LD, Quinn C. Enzyme kinetics determined by single-injection isothermal titration calorimetry. Methods 2015;76:194–200
  • Ragunath C, Manuel SGA, Venkataraman V, et al Probing the role of aromatic residues at the secondary saccharide binding sites of human salivary α-amylase in substrate hydrolysis and bacterial binding. J Mol Biol 2008;384:1232–48
  • Aguirre C, Condado-Morales I, Olguin LF, Costas M. Isothermal titration calorimetry determination of individual rate constants of trypsin catalytic activity. Anal Biochem 2015;479:18–27
  • Kawamura-Konishi Y, Watanabe N, Saito M, et al Isolation of a new phlorotannin, a potent inhibitor of carbohydrate hydrolyzing enzymes, from the brown alga Sargassum patens. J Agric Food Chem 2012;60:5565–70
  • Lo Piparo E, Scheib H, Frei N, et al Flavonoids for controlling starch digestion: structural requirements for inhibiting human α-amylase. J Med Chem 2008;51:3555–61

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.