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Research Article

Isoniazid-induced apoptosis in HepG2 cells: Generation of oxidative stress and Bcl-2 down-regulation

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Pages 242-251 | Received 01 Feb 2010, Accepted 17 Mar 2010, Published online: 30 Apr 2010

References

  • Bai J, Rodriguez AM, Melendez JA, Cederbaum AI. 1999. Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury. J Biol Chem 274:26217–26224.
  • Beutler E, Kuhl W, Saenz GF, Rodriguez W. 1991. Mutation analysis of glucose-6-phosphate dehydrogenase (G6PD) variants in Costa Rica. Hum Genet 87:462–464.
  • Bhadauria S, Singh G, Sinha N, Srivastava S. 2007. Isoniazid induces oxidative stress, mitochondrial dysfunction and apoptosis in Hep G2 cells. Cell Mol Biol (Noisy-le-grand) 53:102–114.
  • Brandon EF, Raap CD, Meijerman I, Beijnen JH, Schellens JH. 2003. An update on in vitro test methods in human hepatic drug biotransformation research: pros and cons. Toxicol Appl Pharmacol 189:233–246.
  • Brent JA, Rumack BH. 1993. Role of free radicals in toxic hepatic injury. I. Free radical biochemistry. J Toxicol Clin Toxicol 31:139–171.
  • Chowdhury A, Santra A, Bhattacharjee K, Ghatak S, Saha DR, Dhali GK. 2006. Mitochondrial oxidative stress and permeability transition in isoniazid and rifampicin induced liver injury in mice. J Hepatol 45:117–126.
  • Cox AG, Hampton MB. 2007. Bcl-2 over-expression promotes genomic instability by inhibiting apoptosis of cells exposed to hydrogen peroxide. Carcinogenesis 28:2166–2171.
  • Gurumurthy P, Ramachandran G, Vijayalakshmi S, Kumar AK, Venkatesan P, Chandrasekaran V, Vjayasekaran V, Kumaraswami V, Prabhakar R. 1999. Bioavailability of rifampicin, isoniazid and pyrazinamide in a triple drug formulation: comparison of plasma and urine kinetics. Int J Tuberc Lung Dis 3:119–125.
  • Hewitt NJ, Hewitt P. 2004. Phase I and II enzyme characterization of two sources of HepG2 cell lines. Xenobiotica 34:243–256.
  • Huang YS, Chern HD, Su WJ, Wu JC, Chang SC, Chiang CH, Chang FY, Lee SD. 2003. Cytochrome P450 2E1 genotype and the susceptibility to antituberculosis drug-induced hepatitis. Hepatology 37:924–930.
  • Huang YS, Chern HD, Su WJ, Wu JC, Lai SL, Yang SY, Chang FY, Lee SD. 2002. Polymorphism of the N-acetyltransferase 2 gene as a susceptibility risk factor for antituberculosis drug-induced hepatitis. Hepatology 35:883–889.
  • Kakkar P, Das B, Viswanathan PN. 1984. A modified spectrophotometric assay of superoxide dismutase. Indian J Biochem Biophys 21:130–132.
  • Knasmuller S, Cavin C, Chakraborty A, Darroudi F, Majer BJ, Huber WW, Ehrlich VA. 2004a. Structurally related mycotoxins ochratoxin A, ochratoxin B, and citrinin differ in their genotoxic activities and in their mode of action in human-derived liver (HepG2) cells: implications for risk assessment. Nutr Cancer 50:190–197.
  • Knasmuller S, Mersch-Sundermann V, Kevekordes S, Darroudi F, Huber WW, Hoelzl C, Bichler J, Majer BJ. 2004b. Use of human-derived liver cell lines for the detection of environmental and dietary genotoxicants; current state of knowledge. Toxicology 198:315–328.
  • Lee YJ, Chen JC, Amoscato AA, Bennouna J, Spitz DR, Suntharalingam M, Rhee JG. 2001. Protective role of Bcl2 in metabolic oxidative stress-induced cell death. J Cell Sci 114:677–684.
  • Leopold JA, Zhang YY, Scribner AW, Stanton RC, Loscalzo J. 2003. Glucose-6-phosphate dehydrogenase overexpression decreases endothelial cell oxidant stress and increases bioavailable nitric oxide. Arterioscler Thromb Vasc Biol 23:411–417.
  • Lowry OH, Rosebrough NZ, Farr AL, Randall RJ. 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275.
  • Miller FJ Jr, Gutterman DD, Rios CD, Heistad DD, Davidson BL. 1998. Superoxide production in vascular smooth muscle contributes to oxidative stress and impaired relaxation in atherosclerosis. Circ Res 82:1298–1305.
  • Nishikimi M, Appaji N, Yagi K. 1972. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 46:849–854.
  • Nogae I, Johnston M. 1990. Isolation and characterization of the ZWF1 gene of Saccharomyces cerevisiae, encoding glucose-6-phosphate dehydrogenase. Gene 96:161–169.
  • Pandolfi PP, Sonati F, Rivi R, Mason P, Grosveld F, Luzzatto L. 1995. Targeted disruption of the housekeeping gene encoding glucose 6-phosphate dehydrogenase (G6PD): G6PD is dispensable for pentose synthesis but essential for defense against oxidative stress. EMBO J 14:5209–5215.
  • Peretti E, Karlaganis G, Lauterburg BH. 1987. Increased urinary excretion of toxic hydrazino metabolites of isoniazid by slow acetylators. Effect of a slow-release preparation of isoniazid. Eur J Clin Pharmacol 33:283–286.
  • Pugazhenthi S, Nesterova A, Jambal P, Audesirk G, Kern M, Cabell L, Eves E, Rosner MR, Boxer LM, Reusch JE. 2003. Oxidative stress-mediated down-regulation of bcl-2 promoter in hippocampal neurons. J Neurochem 84:982–996.
  • Salvemini F, Franze A, Iervolino A, Filosa S, Salzano S, Ursini MV. 1999. Enhanced glutathione levels and oxidoresistance mediated by increased glucose-6-phosphate dehydrogenase expression. J Biol Chem 274:2750–2757.
  • Santhosh S, Sini TK, Anandan R, Mathew PT. 2006. Effect of chitosan supplementation on antitubercular drugs-induced hepatotoxicity in rats. Toxicology 219:53–59.
  • Sarich TC, Adams SP, Petricca G, Wright JM. 1999. Inhibition of isoniazid-induced hepatotoxicity in rabbits by pretreatment with an amidase inhibitor. J Pharmacol Exp Ther 289:695–702.
  • Sarich TC, Youssefi M, Zhou T, Adams SP, Wall RA, Wright JM. 1996. Role of hydrazine in the mechanism of isoniazid hepatotoxicity in rabbits. Arch Toxicol 70:835–840.
  • Sarich TC, Zhou T, Adams SP, Bain AI, Wall RA, Wright JM. 1995. A model of isoniazid-induced hepatotoxicity in rabbits. J Pharmacol Toxicol Methods 34:109–116.
  • Scholl A, Anders F. 1973. Electrophoretic variation of enzyme proteins in platyfish and swordtails (poeciliidae, teleostei). Arch Genet (Zur) 46:121–129.
  • Schwab CE, Tuschl H. 2003. In vitro studies on the toxicity of isoniazid in different cell lines. Hum Exp Toxicol 22:607–615.
  • Sinha AK. 1971. Address by dr. A.K.N. Sinha President-Elect, I.M.A. J Indian Med Assoc 56:161–174.
  • Slater AF, Stefan C, Nobel I, van den Dobbelsteen DJ, Orrenius S. 1995. Signalling mechanisms and oxidative stress in apoptosis. Toxicol Lett 82:149–153.
  • Socci DJ, Bjugstad KB, Jones HC, Pattisapu JV, Arendash GW. 1999. Evidence that oxidative stress is associated with the pathophysiology of inherited hydrocephalus in the H-Tx rat model. Exp Neurol 155:109–117.
  • Sodhi CP, Rana SV, Mehta SK, Vaiphei K, Attri S, Thakur S, Mehta S. 1996. Study of oxidative stress in isoniazid-induced hepatic injury in young rats with and without protein-energy malnutrition. J Biochem Toxicol 11:139–146.
  • Triglia T, Burns GF. 1983. A method for in vitro clearance of mycoplasma from human cell lines. J Immunol Methods 64:133–139.
  • Vulliamy T, Mason P, Luzzatto L. 1992. The molecular basis of glucose-6-phosphate dehydrogenase deficiency. Trends Genet 8:138–143.
  • Yue J, Peng RX, Yang J, Kong R, Liu J. 2004. CYP2E1 mediated isoniazid-induced hepatotoxicity in rats. Acta Pharmacol Sin 25:699–704.
  • Zhang Y, Young DB. 1993 Molecular mechanisms of isoniazid: a drug at the front line of tuberculosis control. Trends Microbiol 1:109–113.

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