543
Views
32
CrossRef citations to date
0
Altmetric
Research Article

Studying the effect of LPS on cytotoxicity and apoptosis in PC12 neuronal cells: Role of Bax, Bcl-2, and Caspase-3 protein expression

, &
Pages 316-320 | Received 21 Jan 2010, Accepted 14 Apr 2010, Published online: 03 Jun 2010

References

  • Arai H, Furuya T, Yasuda T, Miura M, Mizuno Y, Mochizuki H. 2004. Neurotoxic effects of lipopolysaccharide on nigral dopaminergic neurons are mediated by microglial activation, interleukin-1beta, and expression of caspase-11 in mice. J Biol Chem 279:51647–41653.
  • Barger SW, Basile AS. 2001. Activation of microglia by secreted amyloid precursor protein evokes release of glutamate by cystine exchange and attenuates synaptic function. J Neurochem 76:846–854.
  • Chao CC, Hu S, Molitor TW, Shaskan EG, Peterson PK. 1992. Activated microglia mediate neuronal cell injury via a nitric oxide mechanism. J Immunol 149:2736–2741.
  • Dawson VL, Brahmbhatt HP, Mong JA, Dawson TM. 1994. Expression of inducible nitric oxide synthase causes delayed neurotoxicity in primary mixed neuronal-glial cortical cultures. Neuropharmacology 33:1425–1430.
  • Emre Y, Hurtaud C, Nubel T, Criscuolo F, Ricquier D, Cassard-Doulcier AM. 2007. Mitochondria contribute to LPS-induced MAPK activation via uncoupling protein UCP2 in macrophages. Biochem J 402:271–278.
  • Fadeel B, Orrenius S. 2005. Apoptosis: a basic biological phenomenon with wide-ranging implications in human disease. J Intern Med 258:479–517.
  • Friedlander RM. 2003. Apoptosis and caspases in neurodegenerative diseases. N Engl J Med 348:1365–1375.
  • Greene LA, Tischler AS. 1976. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA 73:2424–2428.
  • Heneka MT, Loschmann PA, Gleichmann M, Weller M, Schulz JB, Wullner U, Klockgether T. 1998. Induction of nitric oxide synthase and nitric oxide-mediated apoptosis in neuronal PC12 cells after stimulation with tumor necrosis factor-alpha/lipopolysaccharide. J Neurochem 71:88–94.
  • Johansson S, Bohman S, Radesäter AC, Oberg C, Luthman J. 2005. Salmonella lipopolysaccharide (LPS) mediated neurodegeneration in hippocampal slice cultures. Neurotox Res 8:207–220.
  • Kermer P, Liman J, Weishaupt JH, Bahr M. 2004. Neuronal apoptosis in neurodegenerative diseases: from basic research to clinical application. Neurodegener Dis 1:9–19.
  • Kim WG, Mohney RP, Wilson B, Jeohn GH, Liu B, Hong JS. 2000. Regional difference in susceptibility to lipopolysaccharide-induced neurotoxicity in the rat brain: role of microglia. J Neurosci 20:6309–6316.
  • Lavrik IN, Golks A, Krammer PH. 2005. Caspases: pharmacological manipulation of cell death. J Clin Invest 115:2665–2672.
  • Li Y, Liu L, Barger SW, Griffin WS. 2003. Interleukin-1 mediates pathological effects of microglia on tau phosphorylation and on synaptophysin synthesis in cortical neurons through a p38-MAPK pathway. J Neurosci 23:1605–1611.
  • Mosmann T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 65:55–63.
  • Qian L, Block ML, Wei SJ, Lin CF, Reece J, Pang H, Wilson B, Hong JS, Flood PM. 2006. Interleukin-10 protects lipopolysaccharide-induced neurotoxicity in primary midbrain cultures by inhibiting the function of NADPH oxidase. J Pharmacol Exp Ther 319:44–52.
  • Segura-Aguilar J, Kostrzewa RM. 2006. Neurotoxins and neurotoxicity mechanisms. An overview. Neurotox Res 10:263–287.
  • Selznick LA, Zheng TS, Flavell, RA, Rakic P, Roth KA. 2000. Amyloid beta-induced neuronal death is bax-dependent but caspase-independent. J Neuropathol Exp Neurol 59:271–279.
  • Sharifi AM, Baniasadi S, Jorjani M, Rahimi F, Bakhshayesh M. 2002. Investigation of acute lead poisoning on apoptosis in rat hippocampus in vivo. Neurosci Lett 329:45–48.
  • Sharifi AM, Eslami H, Larijani B, Davoodi J. 2009. Involvement of caspase-8, -9, and -3 in high glucose-induced apoptosis in PC12 cells. Neurosci Lett 459:47–51.
  • Sharifi AM, Mousavi, SH. 2008. Studying the effects of lead on DNA fragmentation and proapoptotic Bax and antiapoptotic Bcl-2 protein expression in PC12 cells. Toxicol Mech Meth 18:75–79.
  • Sharifi AM, Mousavi SH, Bakhshayesh M, Tehrani FK, Mahmoudian M, Oryan S. 2005. Study of correlation between lead-induced cytotoxicity and nitric oxide production in PC12 cells. Toxicol Lett 160:43–48.
  • Sharifi AM, Mousavi SH, Farhadi M, Larijani B. 2007. Study of high glucose-induced apoptosis in PC12 cells: role of bax protein. J Pharmacol Sci 104:258–262.
  • Sharifi AM, Schiffrin EL. 1998. Apoptosis in vasculature of spontaneously hypertensive rats: effect of an angiotensin converting enzyme inhibitor and a calcium channel antagonist. Am J Hypertens 11:1108–1116.
  • Shibata H, Katsuki H, Nishiwaki M, Kume T, Kaneko S, Akaike A. 2003. Lipopolysaccharide-induced dopaminergic cell death in rat midbrain slice cultures: role of inducible nitric oxide synthase and protection by indomethacin. J Neurochem 86:1201–1212.
  • Wang X, Chen S, Ma G, Ye M, Lu G. 2005. Involvement of proinflammatory factors, apoptosis, caspase-3 activation and Ca2+ disturbance in microglia activation-mediated dopaminergic cell degeneration. Mech Ageing Dev 126:1241–1254.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.