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APPLICATION NOTE

Quantitative large scale gene expression profiling from human stem cell culture micro samples using multiplex pre-amplification

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Pages 84-91 | Received 20 Jan 2015, Accepted 17 Apr 2015, Published online: 03 Aug 2015

References

  • Adewumi, O., Aflatoonian, B., Ahrlund-Richter, L., Amit, M., Andrews, P.W., Beighton, G., et al. (2007) Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nat Biotechnol 25:803–816
  • Bernardo, V., Ribeiro Pinto, L.F. and Albano, R.M. (2013) Gene expression analysis by real-time PCR: experimental demonstration of PCR detection limits. Anal Biochem 432:131–133
  • Bock, C., Kiskinis, E., Verstappen, G., Gu, H., Boulting, G., Smith, Z.D., et al. (2011) Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines. Cell 144:439–452
  • Brambrink, T., Foreman, R., Welstead, G.G., Lengner, C.J., Wernig, M., Suh, H., et al. (2008) Sequential expression of pluripotency markers during direct reprogramming of mouse somatic cells. Cell Stem Cell 2:151–159
  • Dang, S.M., Gerecht-Nir, S., Chen, J., Itskovitz-Eldor, J. and Zandstra, P.W. (2004) Controlled, scalable embryonic stem cell differentiation culture. Stem Cells 22:275–282
  • De Los Angeles, A., Loh, Y.H., Tesar, P.J. and Daley, G.Q. (2012) Accessing naive human pluripotency. Curr Opin Genet Dev 22:272–282
  • Esmailpour, T. and Huang, T. (2012) TBX3 promotes human embryonic stem cell proliferation and neuroepithelial differentiation in a differentiation stage-dependent manner. Stem Cells 30:2152–2163
  • Kibschull, M., Mileikovsky, M., Michael, I.P., Lye, S.J. and Nagy, A. (2011) Human embryonic fibroblasts support single cell enzymatic expansion of human embryonic stem cells in xeno-free cultures. Stem Cell Res 6:70–82
  • Kralj, J.G. and Salit, M.L. (2013) Characterization of in vitro transcription amplification linearity and variability in the low copy number regime using External RNA Control Consortium (ERCC) spike-ins. Anal Bioanal Chem 405:315–320
  • Maddah, M., Shoukat-Mumtaz, U., Nassirpour, S. and Loewke, K. (2014) A system for automated, noninvasive, morphology-based evaluation of induced pluripotent stem cell cultures. J Lab Autom 19:454–460
  • Marti, M., Mulero, L., Pardo, C., Morera, C., Carrio, M., Laricchia-Robbio, L., et al. (2013) Characterization of pluripotent stem cells. Nat Protoc 8:223–253
  • Outten, J.T., Gadue, P., French, D.L. and Diamond, S.L. (2012) High-throughput screening assay for embryoid body differentiation of human embryonic stem cells. Curr Protoc Stem Cell Biol Chapter 1:Unit 1D 6
  • Pal, R., Mamidi, M.K., Das, A.K., Rao, M. and Bhonde, R. (2013) Development of a multiplex PCR assay for characterization of embryonic stem cells. Methods Mol Biol 1006:147–166
  • Song, H., Suehiro, J., Kanki, Y., Kawai, Y., Inoue, K., Daida, H., et al. (2009) Critical role for GATA3 in mediating Tie2 expression and function in large vessel endothelial cells. J Biol Chem 284:29109–29124
  • Tellinghuisen, J. and Spiess, A.N. (2015) Absolute copy number from the statistics of the quantification cycle in replicate quantitative polymerase chain reaction experiments. Anal Chem 87:1889–1895
  • Ungrin, M.D., Joshi, C., Nica, A., Bauwens, C. and Zandstra, P.W. (2008) Reproducible, ultra high-throughput formation of multicellular organization from single cell suspension-derived human embryonic stem cell aggregates. PLoS One 3:e1565
  • Vandesompele, J., De Preter, K., Pattyn, F., Poppe, B., Van Roy, N., De Paepe, A., et al. (2002) Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol 3:RESEARCH0034
  • Werth, D., Grassi, G., Konjer, N., Dapas, B., Farra, R., Giansante, C., et al. (2010) Proliferation of human primary vascular smooth muscle cells depends on serum response factor. Eur J Cell Biol 89:216–224
  • Williams, R., Schuldt, B. and Muller, F.J. (2011) A guide to stem cell identification: progress and challenges in system-wide predictive testing with complex biomarkers. Bioessays 33:880–890
  • Woltjen, K., Hamalainen, R., Kibschull, M., Mileikovsky, M. and Nagy, A. (2011) Transgene-free production of pluripotent stem cells using piggyBac transposons. Methods Mol Biol 767:87–103
  • Yang, L., Soonpaa, M.H., Adler, E.D., Roepke, T.K., Kattman, S.J., Kennedy, M., et al. (2008) Human cardiovascular progenitor cells develop from a KDR + embryonic-stem-cell-derived population. Nature 453:524–528
  • Yu, Y., Liu, L., Xie, N., Xue, H., Fazli, L., Buttyan, R., et al. (2013) Expression and function of the progesterone receptor in human prostate stroma provide novel insights to cell proliferation control. J Clin Endocrinol Metab 98:2887–2896
  • Zhang, P., Li, J., Tan, Z., Wang, C., Liu, T., Chen, L., et al. (2008) Short-term BMP-4 treatment initiates mesoderm induction in human embryonic stem cells. Blood 111:1933–1941

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