791
Views
4
CrossRef citations to date
0
Altmetric
Original Articles

Carrier free immobilization and characterization of trypsin

&
Pages 140-144 | Received 29 Aug 2013, Accepted 05 Oct 2013, Published online: 06 Nov 2013

References

  • Abbas A, Vercaigne-Marko D, Supiot P, Bocquet B, Vivien C, Guillochon D. 2009. Covalent attachment of trypsin on plasma polymerized allylamine. Colloid Surface B. 73:315–324.
  • Bradford M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 72:248–254.
  • Brady D, Jordaan J. 2009. Advances in enzyme immobilisation. Biotechnol Lett. 31:1639–1650.
  • Bryjak J, Kolarz BN. 1998. Immobilisation of trypsin on acrylic copolymers. Process Biochem. 33:409–417.
  • Cabana H, Jones JP, Agathos SN. 2007. Preparation and characterization of cross-linked laccase aggregates and their application to the elimination of endocrine disrupting chemicals. J Biotechnol. 132:23–31.
  • Chang TMS. 1971. Stabilization of enzymes by microencapsulation with a concentrated protein solution or by microencapsulation followed by cross linking with glutaraldehyde. Biochem Biophys Res Commun. 44:1531–1536.
  • Daglioglu C, Zihnioglu F. 2012. Covalent immobilization of trypsin on glutaraldehyde-activated silica for protein fragmentation. Artif Cell Blood Sub. 40:378–384.
  • Dalal S, Kapoor M, Gupta MN. 2007. Preparation and characterization of combi-CLEAs catalyzing multiple non-cascade reactions. J Mol Catal B Enzym. 44:128–132.
  • Gupta P, Dutt K, Misra S, Raghuwanshi S, Saxena RK. 2009. Characterization of cross-linked immobilized lipase from thermophilic mould Thermomyces lanuginosa using glutaraldehyde. Bioresour Technol. 100:4074–4076.
  • Kartal F, Janssen MHA, Hollmann F, Sheldon RA, Kılınc A. 2011. Improved esterification activity of Candida rugosa lipase in organic solvent by immobilization as Cross-linked enzyme aggregates (CLEAs). J Mol Catal B Enzym. 71:85–89.
  • Ktari N, Ben Khaled H, Nasri R, Jellouli K, Ghorbel S, Nasri M. 2012. Trypsin from zebra blenny (Salaria basilisca) viscera: purification, characterisation and potential application as a detergent additive. Food Chem. 130:467–474.
  • López-Serrano P, Cao L, van Rantwijk F, Sheldon RA. 2002. Cross-linked enzyme aggregates with enhanced activity: application to lipases. Biotechnol Lett. 24:1379–1383.
  • Massolini G, Calleri E. 2005. Immobilized trypsin systems coupled on-line to separation methods: recent developments and analytical applications. J Sep Sci. 28:7–21.
  • Migneault I, Dartiguenave C, Bertrand MJ, Waldron KC. 2004. Glutaraldehyde: behavior in aqueous solution, reaction with proteins, and application to enzyme crosslinking. Biotechniques. 37:790–802.
  • Monteiro FMF, Silva GMM, Silva JBR, Porto CS, Carvalho LB Jr , Lima Filho JL, et al. 2007. Immobilization of trypsin on polysaccharide film from Anacardium occidentale L. and its application as cutaneous dressing. Process Biochem. 42: 884–888.
  • Park J, Kim M, Lee D, Lee K, Min J, Kim Y. 2010. Immobilization of the cross-linked para-nitrobenzyl esterase of Bacillus subtilis aggregates onto magnetic beads. Process Biochem. 45:259–263.
  • Roessl U, Nahalka J, Nidetzky B. 2010. Carrier-free immobilized enzymes for biocatalysis. Biotechnol Lett. 32:341–350.
  • Sangeetha K, Abraham TE. 2008. Preparation and characterization of cross-linked enzyme aggregates (CLEA) of subtilisin for controlled release applications. Int J Biol Macromol. 43:314–319.
  • Schoevaart R, Wolbers MW, Golubovic M, Ottens M, Kieboom APG, Van Rantwijk F, et al. 2004. Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs). Biotechnol Bioeng. 87:754–762.
  • Shah S, Sharma A, Gupta MN. 2006. Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder. Anal Biochem. 351:207–213.
  • Sheldon RA. 2007. Cross-linked enzyme aggregates (CLEAs): stable and recyclable biocatalysts. Biochem Soc Trans. 35:1583–1587.
  • Sheldon RA. 2011. Cross-linked enzyme aggregates as industrial biocatalysts. Org Process Res Dev. 15:213–223.
  • Wu J, Luan M, Zhao J. 2006. Trypsin immobilization by direct adsorption on metal ion chelated macroporous chitosan-silica gel beads. Int J Biol Macromol. 39:185–191.
  • Yu HW, Chen H, Wang X, Yang YY, Ching CB. 2006. Cross- linked enzyme aggregates (CLEAs) with controlled particles: Application to Candida rugosa lipase. J Mol Catal B Enzym. 43: 124–127.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.