Isolation of biologically active and morphologically intact exosomes from plasma of patients with cancer

References

• Cocucci E, Meldolesi J. Ectosomes and exosomes: shedding the confusion between extracellular vesicles. Trends Cell Biol. 2015; 25: 364–72. doi: http://dx.doi.org/10.1016/j.tcb.2015.01.004.
   Google Scholar
• Raposo G, Stoorvogel W. Extracellular vesicles: exosomes, microvesicles, and friends. J Cell Biol. 2013; 200: 373–83. doi: http://dx.doi.org/10.1083/jcb.201211138.
   Google Scholar
• Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, etal. Proteomic and immunologic analyses of brain tumor exosomes. FASEB J. 2009; 23: 1541–57. doi: http://dx.doi.org/10.1096/fj.08-122184.
   Google Scholar
• Brinton LT, Sloane HS, Kester M, Kelly KA. Formation and role of exosomes in cancer. Cell Mol Life Sci. 2015; 72: 659–71. doi: http://dx.doi.org/10.1007/s00018-014-1764-3.
   Google Scholar
• Peinado H, Aleckovic M, Lavotshkin S, Matei I, Costa-Silva B, Moreno-Bueno G, etal. Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med. 2012; 18: 883–91. doi: http://dx.doi.org/10.1038/nm.2753.
   Google Scholar
• Szczepanski MJ, Szajnik M, Welsh A, Whiteside TL, Boyiadzis M. Blast-derived microvesicles in sera from patients with acute myeloid leukemia suppress natural killer cell function via membrane-associated transforming growth factor-beta1. Haematologica. 2011; 96: 1302–9. doi: http://dx.doi.org/10.3324/haematol.2010.039743.
   Google Scholar
• De Toro J, Herschlik L, Waldner C, Mongini C. Emerging roles of exosomes in normal and pathological conditions: new insights for diagnosis and therapeutic applications. Front Immunol. 2015; 6: 203. doi: http://dx.doi.org/10.3389/fimmu.2015.00203.
   Google Scholar
• Thery C, Amigorena S, Raposo G, Clayton A. Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol. 2006. Chapter 3:Unit 3.22. doi: http://dx.doi.org/10.1002/0471143030.cb0322s30.
   Google Scholar
• Montecalvo A, Larregina AT, Morelli AE. Methods of purification of CTL-derived exosomes. Methods Mol Biol. 2014; 1186: 87–102. doi: http://dx.doi.org/10.1007/978-1-4939-1158-5_7.
   Google Scholar
• Taylor DD, Shah S. Methods of isolating extracellular vesicles impact down-stream analyses of their cargoes. Methods. 2015; 87: 3–10. doi: http://dx.doi.org/10.1016/j.ymeth.2015.02.019.
   Google Scholar
• Taylor DD, Chou IN, Black PH. Isolation of plasma membrane fragments from cultured murine melanoma cells. Biochem Biophys Res Commun. 1983; 113: 470–6.
   PubMed Web of Science ®Google Scholar
• Hong CS, Muller L, Boyiadzis M, Whiteside TL. Isolation and characterization of CD34+ blast-derived exosomes in acute myeloid leukemia. PLoS One. 2014; 9: e103310. doi: http://dx.doi.org/10.1371/journal.pone.0103310.
   Google Scholar
• Saze Z, Schuler PJ, Hong CS, Cheng D, Jackson EK, Whiteside TL. Adenosine production by human B cells and B cell-mediated suppression of activated T cells. Blood. 2013; 122: 9–18. doi: http://dx.doi.org/10.1182/blood-2013-02-482406.
   Google Scholar
• Muller L, Hong CS, Stolz DB, Watkins SC, Whiteside TL. Isolation of biologically-active exosomes from human plasma. J Immunol Methods. 2014; 411: 55–65. doi: http://dx.doi.org/10.1016/j.jim.2014.06.007.
   Google Scholar
• Strauss L, Bergmann C, Whiteside TL. Human circulating CD4+CD25highFoxp3+ regulatory T cells kill autologous CD8+ but not CD4+ responder cells by Fas-mediated apoptosis. J Immunol. 2009; 182: 1469–80.
   PubMed Web of Science ®Google Scholar
• Wieckowski EU, Visus C, Szajnik M, Szczepanski MJ, Storkus WJ, Whiteside TL. Tumor-derived microvesicles promote regulatory T cell expansion and induce apoptosis in tumor-reactive activated CD8+ T lymphocytes. J Immunol. 2009; 183: 3720–30. doi: http://dx.doi.org/10.4049/jimmunol.0900970.
   Google Scholar
• Hong CS, Muller L, Whiteside TL, Boyiadzis M. Plasma exosomes as markers of therapeutic response in patients with acute myeloid leukemia. Front Immunol. 2014; 5: 160. doi: http://dx.doi.org/10.3389/fimmu.2014.00160.
   Google Scholar
• Kim JW, Wieckowski E, Taylor DD, Reichert TE, Watkins S, Whiteside TL. Fas ligand-positive membranous vesicles isolated from sera of patients with oral cancer induce apoptosis of activated T lymphocytes. Clin Cancer Res. 2005; 11: 1010–20.
   PubMed Web of Science ®Google Scholar
• Whiteside TL. Immune modulation of T-cell and NK (natural killer) cell activities by TEXs (tumour-derived exosomes). Biochem Soc Trans. 2013; 41: 245–51. doi: http://dx.doi.org/10.1042/BST20120265.
   Google Scholar
• Taylor DD, Zacharias W, Gercel-Taylor C. Exosome isolation for proteomic analyses and RNA profiling. Methods Mol Biol. 2011; 728: 235–46. doi: http://dx.doi.org/10.1007/978-1-61779-068-3_15.
   Google Scholar
• Chen C, Skog J, Hsu CH, Lessard RT, Balaj L, Wurdinger T, etal. Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip. 2010; 10: 505–11. doi: http://dx.doi.org/10.1039/b916199f.
   Google Scholar
• Tauro BJ, Greening DW, Mathias RA, Ji H, Mathivanan S, Scott AM, etal. Comparison of ultracentrifugation, density gradient separation, and immunoaffinity capture methods for isolating human colon cancer cell line LIM1863-derived exosomes. Methods. 2012; 56: 293–304. doi: http://dx.doi.org/10.1016/j.ymeth.2012.01.002.
   Google Scholar
• Kowal J, Tkach M, Thery C. Biogenesis and secretion of exosomes. Curr Opin Cell Biol. 2014; 29: 116–25. doi: http://dx.doi.org/10.1016/j.ceb.2014.05.004.
   Google Scholar
• Martins VR, Dias MS, Hainaut P. Tumor-cell-derived microvesicles as carriers of molecular information in cancer. Curr Opin Oncol. 2013; 25: 66–75. doi: http://dx.doi.org/10.1097/CCO.0b013e32835b7c81.
   Google Scholar
• Muller L, Muller-Haegele S, Mitsuhashi M, Gooding W, Okada H, Whiteside TL. Exosomes isolated from plasma of glioma patients enrolled in a vaccination trial reflect antitumor immune activity and might predict survival. Oncoimmunology. 2015; 4: e1008347. doi: http://dx.doi.org/10.1080/2162402X.2015.1008347.
   Google Scholar
• Khan S, Aspe JR, Asumen MG, Almaguel F, Odumosu O, Acevedo-Martinez S, etal. Extracellular, cell-permeable survivin inhibits apoptosis while promoting proliferative and metastatic potential. Br J Cancer. 2009; 100: 1073–86. doi: http://dx.doi.org/10.1038/sj.bjc.6604978.
   Google Scholar
• Welton JL, Webber JP, Botos LA, Jones M, Clayton A. Ready-made chromatography columns for extracellular vesicle isolation from plasma. J Extracell Vesicles. 2015; 4: 27269. doi: http://dx.doi.org/10.3402/jev.v4.27269.
   Google Scholar
• Boing AN, van der Pol E, Grootemaat AE, Coumans FA, Sturk A, Nieuwland R. Single-step isolation of extracellular vesicles by size-exclusion chromatography. J Extracell Vesicles. 2014; 3: 23430. doi: http://dx.doi.org/10.3402/jev.v3.23430.
   Google Scholar
• de Menezes-Neto A, Saez MJ, Lozano-Ramos I, Segui-Barber J, Martin-Jaular L, Ullate JM, etal. Size-exclusion chromatography as a stand-alone methodology identifies novel markers in mass spectrometry analyses of plasma-derived vesicles from healthy individuals. J Extracell Vesicles. 2015; 4: 27378. doi: http://dx.doi.org/10.3402/jev.v4.27378.
   Google Scholar
• Bergmann C, Strauss L, Wieckowski E, Czystowska M, Albers A, Wang Y, etal. Tumor-derived microvesicles in sera of patients with head and neck cancer and their role in tumor progression. Head Neck. 2009; 31: 371–80. doi: http://dx.doi.org/10.1002/hed.20968.
   Google Scholar
• Balaj L, Atai NA, Chen W, Mu D, Tannous BA, Breakefield XO, etal. Heparin affinity purification of extracellular vesicles. Sci Rep. 2015; 5: 10266. doi: http://dx.doi.org/10.1038/srep10266.
   Google Scholar
• Andreola G, Rivoltini L, Castelli C, Huber V, Perego P, Deho P, etal. Induction of lymphocyte apoptosis by tumor cell secretion of FasL-bearing microvesicles. J Exp Med. 2002; 195: 1303–16.
   PubMed Web of Science ®Google Scholar
• Webber J, Steadman R, Mason MD, Tabi Z, Clayton A. Cancer exosomes trigger fibroblast to myofibroblast differentiation. Cancer Res. 2010; 70: 9621–30. doi: http://dx.doi.org/10.1158/0008-5472.CAN-10-1722.
   Google Scholar
• Szajnik M, Czystowska M, Szczepanski MJ, Mandapathil M, Whiteside TL. Tumor-derived microvesicles induce, expand and up-regulate biological activities of human regulatory T cells (Treg). PLoS One. 2010; 5: e11469. doi: http://dx.doi.org/10.1371/journal.pone.0011469.
   Google Scholar
• Mrizak D, Martin N, Barjon C, Jimenez-Pailhes AS, Mustapha R, Niki T, etal. Effect of nasopharyngeal carcinoma-derived exosomes on human regulatory T cells. J Natl Cancer Inst. 2015; 107: 363. doi: http://dx.doi.org/10.1093/jnci/dju363.
   Google Scholar
• Whiteside TL. Immune responses to cancer: are they potential biomarkers of prognosis?. Front Oncol. 2013; 3: 107. doi: http://dx.doi.org/10.3389/fonc.2013.00107.
   Google Scholar
• Hwang I, Ki D. Receptor-mediated T cell absorption of antigen presenting cell-derived molecules. Front Biosci (Landmark Ed). 2011; 16: 411–21.
   Web of Science ®Google Scholar
• Lane RE, Korbie D, Anderson W, Vaidyanathan R, Trau M. Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing. Sci Rep. 2015; 5: 7639. doi: http://dx.doi.org/10.1038/srep07639.
   Google Scholar