References
- Gámez-Valero A, Lozano-Ramos SI, Bancu I, Lauzurica-Valdemoros R, Borràs FE. Urinary extracellular vesicles as source of biomarkers in kidney diseases. Front Immunol. 2015; 6: doi: http://dx.doi.org/10.3389/fimmu.2015.00006.
- Gould SJ, Raposo G. As we wait: coping with an imperfect nomenclature for extracellular vesicles. J Extracell Vesicles. 2013; 2(20389): doi: http://dx.doi.org/10.3402/jev.v2i0.20389.
- Zubiri I, Posada-Ayala M, Sanz-Maroto A, Calvo E, Martin-Lorenzo M, Gonzalez-Calero L, etal. Diabetic nephropathy induces changes in the proteome of human urinary exosomes as revealed by label-free comparative analysis. J Proteomics. 2014; 96: 92–102. doi: http://dx.doi.org/10.1016/j.jprot.2013.10.037 [PubMed Abstract].
- Musante L, Tataruch D, Gu D, Liu X, Forsblom C, Groop PH, etal. Proteases and protease inhibitors of urinary extracellular vesicles in diabetic nephropathy. J Diabetes Res. 2015; 2015: 289734, doi: http://dx.doi.org/10.1155/2015/289734 [PubMed CentralFull Text].
- Valadi H, Ekström K, Bossios A, Sjöstrand M, Lee JJ, Lötvall JO. Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol. 2007; 9: 654–9. [PubMed Abstract].
- Dear JW, Street JM, Bailey MA. Urinary exosomes: a reservoir for biomarker discovery and potential mediators of intrarenal signalling. Proteomics. 2013; 13: 1572–80. doi: http://dx.doi.org/10.1002/pmic.201200285 [PubMed Abstract].
- Miranda KC, Bond DT, McKee M, Skog J, Păunescu TG, Da Silva N, etal. Nucleic acids within urinary exosomes/microvesicles are potential biomarkers for renal disease. Kidney Int. 2010; 78: 191–9. doi: http://dx.doi.org/10.1038/ki.2010.106 [PubMed Abstract] [PubMed CentralFull Text].
- Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, etal. The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 2000; 403: 901–6. [PubMed Abstract].
- Patel V, Noureddine L. MicroRNAs and fibrosis. Curr Opin Nephrol Hypertens. 2012; 21: 410–6. doi: http://dx.doi.org/10.1097/MNH.0b013e328354e559 [PubMed Abstract] [PubMed CentralFull Text].
- Noureddine L, Hajarnis S, Patel V. MicroRNAs and polycystic kidney disease. Drug Discov Today Dis Models. 2013; 10: 137–e43.
- Gildea JJ, Seaton JE, Victor KG, Reyes CM, Bigler Wang D, Pettigrew AC, etal. Exosomal transfer from human renal proximal tubule cells to distal tubule and collecting duct cells. Clin Biochem. 2014; 47: 89–94. doi: http://dx.doi.org/10.1016/j.clinbiochem.2014.06.018 [PubMed Abstract].
- Barutta F, Tricarico M, Corbelli A, Annaratone L, Pinach S, Grimaldi S, etal. Urinary exosomal microRNAs in incipient diabetic nephropathy. PLoS One. 2013; 8: e73798. doi: http://dx.doi.org/10.1371/journal.pone.0073798 [PubMed CentralFull Text].
- Miranda KC, Bond DT, Levin JZ, Adiconis X, Sivachenko A, Russ C, etal. Massively parallel sequencing of human urinary exosome/microvesicle RNA reveals a predominance of non-coding RNA. PLoS One. 2014; 9: e96094. doi: http://dx.doi.org/10.1371/journal.pone.0096094 [PubMed CentralFull Text].
- Lv LL, Cao Y, Liu D, Xu M, Liu H, Tang RN, etal. Isolation and quantification of microRNAs from urinary exosomes/microvesicles for biomarker discovery. Int J Biol Sci. 2013; 9: 1021–31. doi: http://dx.doi.org/10.7150/ijbs.6100 [PubMed Abstract] [PubMed CentralFull Text].
- Musante L, Tataruch D, Gu D, Benito-Martin A, Calzaferri G, Aherne S, etal. A simplified method to recover urinary vesicles for clinical applications, and sample banking. Sci Rep. 2014; 4: 7532. doi: http://dx.doi.org/10.1038/srep07532 [PubMed CentralFull Text].
- Eldh M, Lötvall J, Malmhäll C, Ekström K. Importance of RNA isolation methods for analysis of exosomal RNA: evaluation of different methods. Mol Immunol. 2012; 50: 278–86. doi: http://dx.doi.org/10.1016/j.molimm.2012.02.001 [PubMed Abstract].
- Channavajjhala SK, Rossato M, Morandini F, Castagna A, Pizzolo F, Bazzoni F, etal. Optimizing the purification and analysis of miRNAs from urinary exosomes. Clin Chem Lab Med. 2014; 52: 345–54. doi: http://dx.doi.org/10.1515/cclm-2013-0562 [PubMed Abstract].
- Alvarez ML, Khosroheidari M, Kanchi Ravi R, DiStefano JK. Comparison of protein, microRNA, and mRNA yields using different methods of urinary exosome isolation for the discovery of kidney disease biomarkers. Kidney Int. 2012; 82: 1024–32. doi: http://dx.doi.org/10.1038/ki.2012.256 [PubMed Abstract].
- Cheng L, Sun X, Scicluna BJ, Coleman BM, Hill AF. Characterization and deep sequencing analysis of exosomal and non-exosomal miRNA in human urine. Kidney Int. 2014; 86: 433–44. doi: http://dx.doi.org/10.1038/ki.2013.502 [PubMed Abstract].
- Li M, Zeringer E, Barta T, Schageman J, Cheng A, Vlassov AV. Analysis of the RNA content of the exosomes derived from blood serum and urine and its potential as biomarkers. Philos Trans R Soc Lond B Biol Sci. 2014; 369: 20130502. doi: http://dx.doi.org/10.1098/rstb.2013.0502.
- Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, etal. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009; 55: 611–22. doi: http://dx.doi.org/10.1373/clinchem.2008.112797 [PubMed Abstract].
- Hill AF, Pegtel DM, Lambertz U, Leonardi T, O'Driscoll L, Pluchino S, etal. ISEV position paper: extracellular vesicle RNA analysis and bioinformatics. J Extracell Vesicles. 2013; 23: 2. doi: http://dx.doi.org/10.3402/jev.v2i0.22859.
- Parikh VN, Chan SY. Analysis of microRNA niches: techniques to measure extracellular microRNA and intracellular microRNA in situ. Methods Mol Biol. 2013; 1024: 157–72. doi: http://dx.doi.org/10.1007/978-1-62703-4531_12 [PubMed Abstract] [PubMed CentralFull Text].
- Inman BA, Etienne W, Rubin R, Owusu RA, Oliveira TR, Rodriques DB, etal. The impact of temperature and urinary constituents on urine viscosity and its relevance to bladder hyperthermia treatment. Int J Hyperthermia. 2013; 29: 206–10. doi: http://dx.doi.org/10.3109/02656736.2013.775355 [PubMed Abstract].
- Momen-Heravi F, Balaj L, Alian S, Trachtenberg AJ, Hochberg FH, Skog J, etal. Impact of biofluid viscosity on size and sedimentation efficiency of the isolated microvesicles. Front Physiol. 2012; 3: 162. doi: http://dx.doi.org/10.3389/fphys.2012.00162 [PubMed Abstract] [PubMed CentralFull Text].
- Reddi KK. Purification and properties of a ribonuclease in human urine that hydrolyses polycytidylic acid. Prep Biochem. 1977; 7: 283–99. [PubMed Abstract].
- Murai K, Yamanaka M, Akagi K, Anai M, Mukai T, Omae T. Purification and properties of deoxyribonuclease from human urine. Biochim Biophys Acta. 1978; 517: 186–94. [PubMed Abstract].
- Mitchell PJ, Welton J, Staffurth J, Court J, Mason MD, Tabi Z, etal. Can urinary exosomes act as treatment response markers in prostate cancer?. J Transl Med. 2009; 7: 4. doi: http://dx.doi.org/10.1186/1479-5876-7-4 [PubMed CentralFull Text].
- Cvjetkovic A, Lötvall J, Lässer C. The influence of rotor type and centrifugation time on the yield and purity of extracellular vesicles. J Extracell Vesicles. 2014; 3: 23111, doi: http://dx.doi.org/10.3402/jev.v3.23111 [PubMed CentralFull Text].
- Verplaetse H, Verbeeck RM, Verbaeys A, Oosterlinck W. Solubility of calcium oxalate monohydrate and hydroxyapatite in EDTA solutions. J Urol. 1986; 135: 608–11. [PubMed Abstract].
- Hilt EE, McKinley L, Pearce MM, Rosenfeld AB, Zilliox MJ, Mueller ER, etal. Urine is not sterile: use of enhanced urine culture techniques to detect resident bacterial flora in the adult female bladder. J Clin Microbiol. 2014; 52: 871–6. doi: http://dx.doi.org/10.1128/JCM.02876-13 [PubMed Abstract] [PubMed CentralFull Text].
- Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Biochem. 1987; 162: 156–9. [PubMed Abstract].
- Kuschel M. Analysis of messenger RNA using the Agilent 2100 Bioanalyzer and the RNA 6000 LabChip® Kit. 04/2000. Application Note. Available from: https://www.genome.duke.edu/sites/default/files/AgilentmRNA.pdf.
- Neyra C, Paladino J, Le Borgne M. Oxidation of sialic acid using hydrogen peroxide as a new method to tune the reducing activity. Carbohydr Res. 2014; 386: 92–8. doi: http://dx.doi.org/10.1016/j.carres.2014.01.007 [PubMed Abstract].
- T009-Technical bulletin Nanodrop 1000 & 8000; 260/280 and 260/230 Ratios. Wilmington, Delaware: Thermo Scientific. Available from: http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf.
- Ben-Dov IZ, Whalen VM, Goilav B, Max KE, Tuschl T. ell and microvesicle urine microRNA deep sequencing profiles from healthy individuals: observations with potential impact on biomarkers studies. PLoS One. 2016; 11: e0147249. doi: http://dx.doi.org/10.1371/journal.pone.0147249.
- Gonzalez E, Falcon-Perez JM. Cell-derived extracellular vesicles as a platform to identify low-invasive disease biomarkers. Expert Rev Mol Diagn. 2015; 15: 907–23. doi: http://dx.doi.org/10.1586/14737159.1043272 [PubMed Abstract].
- Ramezani A, Devaney JM, Cohen S, Wing MR, Scott R, Knoblach S, etal. Circulating and urinary microRNA profile in focal segmental glomerulosclerosis: a pilot study. Eur J Clin Invest. 2015; 45: 394404. doi: http://dx.doi.org/10.1111/eci.12420.
- Redshaw N, Wilkes T, Whale A, Cowen S, Huggett J, Foy CA. A comparison of miRNA isolation and RTqPCR technologies and their effects on quantification accuracy and repeatability. Biotechniques. 2013; 54: 155–64. doi: http://dx.doi.org/10.2144/000114002 [PubMed Abstract].
- Rekker K, Saare M, Roost AM, Kubo AL, Zarovni N, Chiesi A, etal. Comparison of serum exosome isolation methods for microRNA profiling. Clin Biochem. 2014; 47: 135–8. doi: http://dx.doi.org/10.1016/j.clinbiochem.2013 [PubMed Abstract].
- Zeringer E, Li M, Barta T, Schageman J, Pedersen KW, Neurauter A, etal. Methods for the extraction and RNA profiling of exosomes. World J Methodol. 2013; 3: 11–18. doi: http://dx.doi.org/10.5662/wjm.v3.i1.11 [PubMed Abstract] [PubMed CentralFull Text].
- Crescitelli R, Lässer C, Szabó TG, Kittel A, Eldh M, Dianzani, etal. Distinct RNA profiles in subpopulations of extracellular vesicles: apoptotic bodies, microvesicles and exosomes. J Extracell Vesicles. 2013; 12: 2. doi: http://dx.doi.org/10.3402/jev.v2i0.20677.
- Lewis DA, Brown R, Williams J, White P, Jacobson SK, Marchesi JR, etal. The human urinary microbiome; bacterial DNA in voided urine of asymptomatic adults. Front Cell Infect Microbiol. 2013; 3: 41. doi: http://dx.doi.org/10.3389/fcimb.2013.00041.
- Fouts DE, Pieper R, Szpakowski S, Pohl H, Knoblach S, Suh MJ, etal. Integrated next-generation sequencing of 16S rDNA and metaproteomics differentiate the healthy urine microbiome from asymptomatic bacteriuria in neuropathic bladder associated with spinal cord injury. J Transl Med. 2012; 10: 174. doi: http://dx.doi.org/10.1186/1479-5876-10-174 [PubMed CentralFull Text].
- Frazee BW, Enriquez K, Ng V, Alter H. Abnormal urinalysis results are common, regardless of specimen collection technique, in women without urinary tract infections. J Emerg Med. 2015; 48: 706–11. doi: http://dx.doi.org/10.1016/j.jemermed.2015.02.020 [PubMed Abstract].
- Wolfe AJ, Toh E, Shibata N, Rong R, Kenton K, Fitzgerald M, etal. Evidence of uncultivated bacteria in the adult female bladder. Clin Microbiol. 2012; 50: 1376–83. doi: http://dx.doi.org/10.1128/JCM.05852-11.