Capillary Electrophoresis Mass Spectrometry for Metabolomics: Reflecting on the Next Steps

References

• Soga T , OhashiY, UenoY, NaraokaH, TomitaM, NishiokaT. Quantitative metabolome analysis using capillary electrophoresis mass spectrometry. J. Proteome Res.2(5), 488–494 (2003).
   PubMed Web of Science ®Google Scholar
• Nemes P . Mass spectrometry comes of age for subcellular organelles. Nat. Methods18(10), 1157–1158 (2021).
   PubMed Web of Science ®Google Scholar
• Ramautar R . Resolving volume-restricted metabolomics using sheathless capillary electrophoresis-mass spectrometry. LC GC Eur.30(12), 658–661 (2017).
   Web of Science ®Google Scholar
• Ramautar R . Capillary electrophoresis – mass spectrometry for metabolomics – from metabolite analysis to metabolic profiling. In: Capillary Electrophoresis – Mass Spectrometry for Metabolomics.The Royal Society of Chemistry, London, UK, 1–20 (2018).
   Google Scholar
• Drouin N , van MeverM, ZhangWet al. Capillary electrophoresis-mass spectrometry at trial by metabo-ring: effective electrophoretic mobility for reproducible and robust compound annotation. Anal. Chem.92(20), 14103–14112 (2020).
   PubMed Web of Science ®Google Scholar
• Shanmuganathan M , KroezenZ, GillBet al. The maternal serum metabolome by multisegment injection-capillary electrophoresis-mass spectrometry: a high-throughput platform and standardized data workflow for large-scale epidemiological studies. Nat. Protoc.16(4), 1966–1994 (2021).
   PubMed Web of Science ®Google Scholar
• Harada S , HirayamaA, ChanQet al. Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry. PLoS One13(1), e0191230 (2018).
   PubMed Web of Science ®Google Scholar
• Azab S , LyR, Britz-McKibbinP. Robust method for high-throughput screening of fatty acids by multisegment injection-nonaqueous capillary electrophoresis-mass spectrometry with stringent quality control. Anal. Chem.91(3), 2329–2336 (2019).
   PubMed Web of Science ®Google Scholar
• Latosinska A , SiwyJ, MischakH, FrantziM. Peptidomics and proteomics based on CE-MS as a robust tool in clinical application: the past, the present, and the future. Electrophoresis40(18–19), 2294–2308 (2019).
   PubMed Web of Science ®Google Scholar
• Tofte N , LindhardtM, AdamovaKet al. Early detection of diabetic kidney disease by urinary proteomics and subsequent intervention with spironolactone to delay progression (PRIORITY): a prospective observational study and embedded randomised placebo-controlled trial. Lancet Diabetes Endocrinol.8(4), 301–312 (2020).
   PubMed Web of Science ®Google Scholar
• Mamani-Huanca M , dela Fuente AG, OteroAet al. Enhancing confidence of metabolite annotation in capillary electrophoresis-mass spectrometry untargeted metabolomics with relative migration time and in-source fragmentation. J. Chromatogr. A1635, 461758 (2021).
   PubMed Web of Science ®Google Scholar
• Mischak H , VlahouA, IoannidisJP. Technical aspects and inter-laboratory variability in native peptide profiling: the CE-MS experience. Clin. Biochem.46(6), 432–443 (2013).
   PubMed Web of Science ®Google Scholar
• Kuehnbaum NL , Britz-McKibbinP. New advances in separation science for metabolomics: resolving chemical diversity in a post-genomic era. Chem. Rev.113(4), 2437–2468 (2013).
   PubMed Web of Science ®Google Scholar
• Ramautar R . Capillary electrophoresis-mass spectrometry for clinical metabolomics. Adv. Clin. Chem.74, 1–34 (2016).
   PubMed Web of Science ®Google Scholar
• Lapainis T , RubakhinSS, SweedlerJV. Capillary electrophoresis with electrospray ionization mass spectrometric detection for single-cell metabolomics. Anal. Chem.81(14), 5858–5864 (2009).
   PubMed Web of Science ®Google Scholar
• Portero EP , NemesP. Dual cationic–anionic profiling of metabolites in a single identified cell in a live Xenopus laevis embryo by microprobe CE-ESI-MS. Analyst144(3), 892–900 (2019).
   PubMed Web of Science ®Google Scholar
• Drouin N , PezzattiJ, GagnebinY, Gonzalez-RuizV, SchapplerJ, RudazS. Effective mobility as a robust criterion for compound annotation and identification in metabolomics: toward a mobility-based library. Anal. Chim. Acta1032, 178–187 (2018).
   PubMed Web of Science ®Google Scholar
• van Mever M , SegersK, DrouinNet al. Direct profiling of endogenous metabolites in rat brain microdialysis samples by capillary electrophoresis-mass spectrometry with on-line preconcentration. Microchem. J.156, 104949 (2020).
   Web of Science ®Google Scholar
• Zhang W , HankemeierT, RamautarR. Next-generation capillary electrophoresis-mass spectrometry approaches in metabolomics. Curr. Opin. Biotechnol.43, 1–7 (2017).
   PubMed Web of Science ®Google Scholar
• Zhang W , GuledF, HankemeierT, RamautarR. Profiling nucleotides in low numbers of mammalian cells by sheathless CE-MS in positive ion mode: circumventing corona discharge. Electrophoresis41(5–6), 360–369 (2020).
   PubMed Web of Science ®Google Scholar
• Kawai T , OtaN, OkadaKet al. Ultrasensitive single cell metabolomics by capillary electrophoresis-mass spectrometry with a thin-walled tapered emitter and large-volume dual sample preconcentration. Anal. Chem.91(16), 10564–10572 (2019).
   PubMed Web of Science ®Google Scholar
• Pickens CA , IsenbergSL, CuthbertC, PetritisK. Combining first and second-tier newborn screening in a single assay using high-throughput chip-based capillary electrophoresis coupled to high-resolution mass spectrometry. Clin. Chem.67(12), 1709–1720 (2021).
   PubMed Web of Science ®Google Scholar
• Tubaon RM , HaddadPR, QuirinoJP. Electrokinetic removal of dodecyl sulfate micelles from digested protein samples prior to electrospray-ionization mass spectrometry. Anal. Chem.89(24), 13058–13063 (2017).
   PubMed Web of Science ®Google Scholar
• Sanchez-Lopez E , KammeijerGSM, CregoALet al. Sheathless CE-MS based metabolic profiling of kidney tissue section samples from a mouse model of polycystic kidney disease. Sci. Rep.9(1), 806 (2019).
   PubMedGoogle Scholar
• Ramautar R , ShytiR, SchoenmakerBet al. Metabolic profiling of mouse cerebrospinal fluid by sheathless CE-MS. Anal. Bioanal. Chem.404(10), 2895–2900 (2012).
   PubMed Web of Science ®Google Scholar