References
- Wurm FM. Production of recombinant protein therapeutics in cultivated mammalian cells. Nat Biotechnol 2004; 22:1393 - 8; http://dx.doi.org/10.1038/nbt1026; PMID: 15529164
- Barnes LM, Bentley CM, Dickson AJ. Stability of protein production from recombinant mammalian cells. Biotechnol Bioeng 2003; 81:631 - 9; http://dx.doi.org/10.1002/bit.10517; PMID: 12529877
- Chusainow J, Yang YS, Yeo JH, Toh PC, Asvadi P, Wong NS, Yap MG. A study of monoclonal antibody-producing CHO cell lines: what makes a stable high producer?. Biotechnol Bioeng 2009; 102:1182 - 96; http://dx.doi.org/10.1002/bit.22158; PMID: 18979540
- He L, Winterrowd C, Kadura I, Frye C. Transgene copy number distribution profiles in recombinant CHO cell lines revealed by single cell analyses. Biotechnol Bioeng 2012; 109:1713 - 22; http://dx.doi.org/10.1002/bit.24428; PMID: 22234778
- Jun SC, Kim MS, Hong HJ, Lee GM. Limitations to the development of humanized antibody producing Chinese hamster ovary cells using glutamine synthetase-mediated gene amplification. Biotechnol Prog 2006; 22:770 - 80; http://dx.doi.org/10.1021/bp060004t; PMID: 16739961
- Kim M, O’Callaghan PM, Droms KA, James DC. A mechanistic understanding of production instability in CHO cell lines expressing recombinant monoclonal antibodies. Biotechnol Bioeng 2011; 108:2434 - 46; http://dx.doi.org/10.1002/bit.23189; PMID: 21538334
- Osterlehner A, Simmeth S, Göpfert U. Promoter methylation and transgene copy numbers predict unstable protein production in recombinant Chinese hamster ovary cell lines. Biotechnol Bioeng 2011; 108:2670 - 81; http://dx.doi.org/10.1002/bit.23216; PMID: 21618470
- Yang Y, Mariati, Chusainow J, Yap MG. DNA methylation contributes to loss in productivity of monoclonal antibody-producing CHO cell lines. J Biotechnol 2010; 147:180 - 5; http://dx.doi.org/10.1016/j.jbiotec.2010.04.004; PMID: 20430058
- Paredes V, Park JS, Jeong Y, Yoon J, Baek K. Unstable expression of recombinant antibody during long-term culture of CHO cells is accompanied by histone H3 hypoacetylation. Biotechnol Lett 2013; 35:987 - 93; http://dx.doi.org/10.1007/s10529-013-1168-8; PMID: 23468139
- Swindle CS, Kim HG, Klug CA. Mutation of CpGs in the murine stem cell virus retroviral vector long terminal repeat represses silencing in embryonic stem cells. J Biol Chem 2004; 279:34 - 41; http://dx.doi.org/10.1074/jbc.M309128200; PMID: 14559924
- Lindahl Allen M, Antoniou M. Correlation of DNA methylation with histone modifications across the HNRPA2B1-CBX3 ubiquitously-acting chromatin open element (UCOE). Epigenetics 2007; 2:227 - 36; http://dx.doi.org/10.4161/epi.2.4.5231; PMID: 18032920
- Araki Y, Hamafuji T, Noguchi C, Shimizu N. Efficient recombinant production in mammalian cells using a novel IR/MAR gene amplification method. PLoS One 2012; 7:e41787; http://dx.doi.org/10.1371/journal.pone.0041787; PMID: 22844523
- Dang Q, Auten J, Plavec I. Human beta interferon scaffold attachment region inhibits de novo methylation and confers long-term, copy number-dependent expression to a retroviral vector. J Virol 2000; 74:2671 - 8; http://dx.doi.org/10.1128/JVI.74.6.2671-2678.2000; PMID: 10684282
- Festenstein R, Tolaini M, Corbella P, Mamalaki C, Parrington J, Fox M, Miliou A, Jones M, Kioussis D. Locus control region function and heterochromatin-induced position effect variegation. Science 1996; 271:1123 - 5; http://dx.doi.org/10.1126/science.271.5252.1123; PMID: 8599090
- Girod PA, Nguyen DQ, Calabrese D, Puttini S, Grandjean M, Martinet D, Regamey A, Saugy D, Beckmann JS, Bucher P, et al. Genome-wide prediction of matrix attachment regions that increase gene expression in mammalian cells. Nat Methods 2007; 4:747 - 53; http://dx.doi.org/10.1038/nmeth1076; PMID: 17676049
- Girod PA, Zahn-Zabal M, Mermod N. Use of the chicken lysozyme 5′ matrix attachment region to generate high producer CHO cell lines. Biotechnol Bioeng 2005; 91:1 - 11; http://dx.doi.org/10.1002/bit.20563; PMID: 15889435
- Kwaks TH, Barnett P, Hemrika W, Siersma T, Sewalt RG, Satijn DP, Brons JF, van Blokland R, Kwakman P, Kruckeberg AL, et al. Identification of anti-repressor elements that confer high and stable protein production in mammalian cells. Nat Biotechnol 2003; 21:553 - 8; http://dx.doi.org/10.1038/nbt814; PMID: 12679786
- Pikaart MJ, Recillas-Targa F, Felsenfeld G. Loss of transcriptional activity of a transgene is accompanied by DNA methylation and histone deacetylation and is prevented by insulators. Genes Dev 1998; 12:2852 - 62; http://dx.doi.org/10.1101/gad.12.18.2852; PMID: 9744862
- Zhang F, Frost AR, Blundell MP, Bales O, Antoniou MN, Thrasher AJ. A ubiquitous chromatin opening element (UCOE) confers resistance to DNA methylation-mediated silencing of lentiviral vectors. Mol Ther 2010; 18:1640 - 9; http://dx.doi.org/10.1038/mt.2010.132; PMID: 20588258
- Siegfried Z, Eden S, Mendelsohn M, Feng X, Tsuberi BZ, Cedar H. DNA methylation represses transcription in vivo. Nat Genet 1999; 22:203 - 6; http://dx.doi.org/10.1038/9727; PMID: 10369268
- Senigl F, Plachý J, Hejnar J. The core element of a CpG island protects avian sarcoma and leukosis virus-derived vectors from transcriptional silencing. J Virol 2008; 82:7818 - 27; http://dx.doi.org/10.1128/JVI.00419-08; PMID: 18550662
- Mariati, Ho SC, Yap MG, Yang Y. Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells. Protein Expr Purif 2010; 69:9 - 15; http://dx.doi.org/10.1016/j.pep.2009.08.010; PMID: 19899222
- Mariati, Yeo JH, Koh EY, Ho SC, Yang Y. Insertion of core CpG island element into human CMV promoter for enhancing recombinant protein expression stability in CHO cells. Biotechnol Prog 2014; 30:523 - 34; http://dx.doi.org/10.1002/btpr.1919; PMID: 24789630
- Blazeck J, Alper HS. Promoter engineering: recent advances in controlling transcription at the most fundamental level. Biotechnol J 2013; 8:46 - 58; http://dx.doi.org/10.1002/biot.201200120; PMID: 22890821
- Ho SCL, Yang Y. Identifying and engineering promoters for high level and sustainable therapeutic recombinant protein production in cultured mammalian cells. Biotechnol Lett 2014; 36:1569 - 79; http://dx.doi.org/10.1007/s10529-014-1523-4; PMID: 24737078
- Bailey LA, Hatton D, Field R, Dickson AJ. Determination of Chinese hamster ovary cell line stability and recombinant antibody expression during long-term culture. Biotechnol Bioeng 2012; 109:2093 - 103; http://dx.doi.org/10.1002/bit.24485; PMID: 22896849
- Majocchi S, Aritonovska E, Mermod N. Epigenetic regulatory elements associate with specific histone modifications to prevent silencing of telomeric genes. Nucleic Acids Res 2014; 42:193 - 204; http://dx.doi.org/10.1093/nar/gkt880; PMID: 24071586
- Chan KKK, Wu SM, Nissom PM, Oh SKW, Choo ABH. Generation of high-level stable transgene expressing human embryonic stem cell lines using Chinese hamster elongation factor-1 alpha promoter system. Stem Cells Dev 2008; 17:825 - 36; http://dx.doi.org/10.1089/scd.2007.0233; PMID: 18788934
- Running Deer J, Allison DS. High-level expression of proteins in mammalian cells using transcription regulatory sequences from the Chinese hamster EF-1alpha gene. Biotechnol Prog 2004; 20:880 - 9; http://dx.doi.org/10.1021/bp034383r; PMID: 15176895
- Hejnar J, Hájková P, Plachy J, Elleder D, Stepanets V, Svoboda J. CpG island protects Rous sarcoma virus-derived vectors integrated into nonpermissive cells from DNA methylation and transcriptional suppression. Proc Natl Acad Sci U S A 2001; 98:565 - 9; http://dx.doi.org/10.1073/pnas.98.2.565; PMID: 11209056