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SHORT COMMUNICATION

Transgene expression study of CXCR4 active mutants

Potential prospects in up-modulation of homing and engraftment efficiency of hematopoietic stem/progenitor cells

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Pages 384-388 | Received 22 Mar 2014, Accepted 20 May 2014, Published online: 20 Dec 2014

References

  • Sharma M, Afrin F, Satija N, Tripathi RP, Gangenahalli GU. Stromal-derived factor-1/CXCR4 signaling: indispensable role in homing and engraftment of hematopoietic stem cells in bone marrow. Stem Cells Dev 2011; 20:933-46; PMID:21186999; http://dx.doi.org/10.1089/scd.2010.0263
  • Lapidot T, Kollet O. The essential roles of the chemokine SDF-1 and its receptor CXCR4 in human stem cell homing and repopulation of transplanted immune-deficient NOD/SCID and NOD/SCID/B2m(null) mice. Leukemia 2002; 16:1992-2003; PMID:12357350; http://dx.doi.org/10.1038/sj.leu.2402684
  • Ma Q, Jones D, Borghesani PR, Segal RA, Nagasawa T, Kishimoto T, Bronson RT, Springer TA. Impaired B-lymphopoiesis, myelopoiesis, and derailed cerebellar neuron migration in CXCR4- and SDF-1-deficient mice. Proc Natl Acad Sci U S A 1998; 95:9448-53; PMID:9689100; http://dx.doi.org/10.1073/pnas.95.16.9448
  • Nagasawa T, Hirota S, Tachibana K, Takakura N, Nishikawa S, Kitamura Y, Yoshida N, Kikutani H, Kishimoto T. Defects of B-cell lymphopoiesis and bone-marrow myelopoiesis in mice lacking the CXC chemokine PBSF/SDF-1. Nature 1996; 382:635-8; PMID:8757135; http://dx.doi.org/10.1038/382635a0
  • Peled A, Petit I, Kollet O, Magid M, Ponomaryov T, Byk T, Nagler A, Ben-Hur H, Many A, Shultz L, et al. Dependence of human stem cell engraftment and repopulation of NOD/SCID mice on CXCR4. Science 1999; 283:845-8; PMID:9933168; http://dx.doi.org/10.1126/science.283.5403.845
  • Busillo JM, Benovic JL. Regulation of CXCR4 signaling. Biochim Biophys Acta 2007; 1768:952-63; PMID:17169327; http://dx.doi.org/10.1016/j.bbamem.2006.11.002
  • Peled A, Grabovsky V, Habler L, Sandbank J, Arenzana-Seisdedos F, Petit I, Ben-Hur H, Lapidot T, Alon R. The chemokine SDF-1 stimulates integrin-mediated arrest of CD34(+) cells on vascular endothelium under shear flow. J Clin Invest 1999; 104:1199-211; PMID:10545519; http://dx.doi.org/10.1172/JCI7615
  • Peled A, Kollet O, Ponomaryov T, Petit I, Franitza S, Grabovsky V, Slav MM, Nagler A, Lider O, Alon R, et al. The chemokine SDF-1 activates the integrins LFA-1, VLA-4, and VLA-5 on immature human CD34(+) cells: role in transendothelial/stromal migration and engraftment of NOD/SCID mice. Blood 2000; 95:3289-96; PMID:10828007
  • Lapidot T, Dar A, Kollet O. How do stem cells find their way home? Blood 2005; 106:1901-10; PMID:15890683; http://dx.doi.org/10.1182/blood-2005-04-1417
  • Ghanouni P, Steenhuis JJ, Farrens DL, Kobilka BK. Agonist-induced conformational changes in the G-protein-coupling domain of the beta 2 adrenergic receptor. Proc Natl Acad Sci U S A 2001; 98:5997-6002; PMID:11353823; http://dx.doi.org/10.1073/pnas.101126198
  • Zhang WB, Navenot JM, Haribabu B, Tamamura H, Hiramatu K, Omagari A, Pei G, Manfredi JP, Fujii N, Broach JR, et al. A point mutation that confers constitutive activity to CXCR4 reveals that T140 is an inverse agonist and that AMD3100 and ALX40-4C are weak partial agonists. J Biol Chem 2002; 277:24515-21; PMID:11923301; http://dx.doi.org/10.1074/jbc.M200889200
  • Lozzio BB, Lozzio CB. Properties and usefulness of the original K-562 human myelogenous leukemia cell line. Leuk Res 1979; 3:363-70; PMID:95026; http://dx.doi.org/10.1016/0145-2126(79)90033-X
  • Gupta SK, Pillarisetti K. Cutting edge: CXCR4-Lo: molecular cloning and functional expression of a novel human CXCR4 splice variant. J Immunol 1999; 163:2368-72; PMID:10452968
  • Gossen M, Bonin AL, Freundlieb S, Bujard H. Inducible gene expression systems for higher eukaryotic cells. Curr Opin Biotechnol 1994; 5:516-20; PMID:7765466; http://dx.doi.org/10.1016/0958-1669(94)90067-1
  • Gossen M, Freundlieb S, Bender G, Müller G, Hillen W, Bujard H. Transcriptional activation by tetracyclines in mammalian cells. Science 1995; 268:1766-9; PMID:7792603; http://dx.doi.org/10.1126/science.7792603
  • Bevilacqua MP, Gimbrone MA Jr. Inducible endothelial functions in inflammation and coagulation. Semin Thromb Hemost 1987; 13:425-33; PMID:3122325; http://dx.doi.org/10.1055/s-2007-1003519
  • Rice GE, Munro JM, Bevilacqua MP. Inducible cell adhesion molecule 110 (INCAM-110) is an endothelial receptor for lymphocytes. A CD11/CD18-independent adhesion mechanism. J Exp Med 1990; 171:1369-74; PMID:1691264; http://dx.doi.org/10.1084/jem.171.4.1369
  • M S, F A, Rp T, G G. Regulated expression of CXCR4 constitutive active mutants revealed the up-modulated chemotaxis and up-regulation of genes crucial for CXCR4 mediated homing and engraftment of hematopoietic stem/progenitor cells. J Stem Cells Regen Med 2013; 9:19-27; PMID:24693205
  • Ganju RK, Brubaker SA, Meyer J, Dutt P, Yang Y, Qin S, Newman W, Groopman JE. The alpha-chemokine, stromal cell-derived factor-1alpha, binds to the transmembrane G-protein-coupled CXCR-4 receptor and activates multiple signal transduction pathways. J Biol Chem 1998; 273:23169-75; PMID:9722546; http://dx.doi.org/10.1074/jbc.273.36.23169
  • Kukreja P, Abdel-Mageed AB, Mondal D, Liu K, Agrawal KC. Up-regulation of CXCR4 expression in PC-3 cells by stromal-derived factor-1alpha (CXCL12) increases endothelial adhesion and transendothelial migration: role of MEK/ERK signaling pathway-dependent NF-kappaB activation. Cancer Res 2005; 65:9891-8; PMID:16267013; http://dx.doi.org/10.1158/0008-5472.CAN-05-1293
  • Inukai K, Funaki M, Anai M, Ogihara T, Katagiri H, Fukushima Y, Sakoda H, Onishi Y, Ono H, Fujishiro M, et al. Five isoforms of the phosphatidylinositol 3-kinase regulatory subunit exhibit different associations with receptor tyrosine kinases and their tyrosine phosphorylations. FEBS Lett 2001; 490:32-8; PMID:11172806; http://dx.doi.org/10.1016/S0014-5793(01)02132-9
  • Wysoczynski M, Jankowski K, Miekus K, Kucia M, Janowska-Wieczorek A, Ratajczak J, Ratajczak MZ. Leukemia inhibitory factor: a newly identified chemoattractant and regulator of metastasis of rhabdomyosarcomas and neuroblastomas to bone marrow. Blood (ASH Annual Meeting Abstracts) 2004; 104: Abstract 1278.
  • Kahn J, Byk T, Jansson-Sjostrand L, Petit I, Shivtiel S, Nagler A, Hardan I, Deutsch V, Gazit Z, Gazit D, et al. Overexpression of CXCR4 on human CD34 +progenitors increases their proliferation, migration, and NOD/SCID repopulation. Blood 2004; 103:2942-9; PMID:15070669; http://dx.doi.org/10.1182/blood-2003-07-2607

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