Genetic disruption of USP9X sensitizes colorectal cancer cells to 5-fluorouracil

References

• Letai AG. Diagnosing and exploiting cancer’s addiction to blocks in apoptosis. Nat Rev Cancer 2008; 8:121 - 32; http://dx.doi.org/10.1038/nrc2297; PMID: 18202696
   PubMed Web of Science ®Google Scholar
• Schwickart M, Huang X, Lill JR, Liu J, Ferrando R, French DM, et al. Deubiquitinase USP9X stabilizes MCL1 and promotes tumour cell survival. Nature 2010; 463:103 - 7; http://dx.doi.org/10.1038/nature08646; PMID: 20023629
   PubMed Web of Science ®Google Scholar
• Grasso D, Ropolo A, Lo Ré A, Boggio V, Molejón MI, Iovanna JL, et al. Zymophagy, a novel selective autophagy pathway mediated by VMP1-USP9x-p62, prevents pancreatic cell death. J Biol Chem 2011; 286:8308 - 24; http://dx.doi.org/10.1074/jbc.M110.197301; PMID: 21173155
   PubMed Web of Science ®Google Scholar
• Mouchantaf R, Azakir BA, McPherson PS, Millard SM, Wood SA, Angers A. The ubiquitin ligase itch is auto-ubiquitylated in vivo and in vitro but is protected from degradation by interacting with the deubiquitylating enzyme FAM/USP9X. J Biol Chem 2006; 281:38738 - 47; http://dx.doi.org/10.1074/jbc.M605959200; PMID: 17038327
   PubMed Web of Science ®Google Scholar
• Bernassola F, Karin M, Ciechanover A, Melino G. The HECT family of E3 ubiquitin ligases: multiple players in cancer development. Cancer Cell 2008; 14:10 - 21; http://dx.doi.org/10.1016/j.ccr.2008.06.001; PMID: 18598940
   PubMed Web of Science ®Google Scholar
• Hahn SA, Schutte M, Hoque AT, Moskaluk CA, da Costa LT, Rozenblum E, et al. DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science 1996; 271:350 - 3; http://dx.doi.org/10.1126/science.271.5247.350; PMID: 8553070
   PubMed Web of Science ®Google Scholar
• Dupont S, Mamidi A, Cordenonsi M, Montagner M, Zacchigna L, Adorno M, et al. FAM/USP9x, a deubiquitinating enzyme essential for TGFbeta signaling, controls Smad4 monoubiquitination. Cell 2009; 136:123 - 35; http://dx.doi.org/10.1016/j.cell.2008.10.051; PMID: 19135894
   PubMed Web of Science ®Google Scholar
• Vucic D, Dixit VM, Wertz IE. Ubiquitylation in apoptosis: a post-translational modification at the edge of life and death. Nat Rev Mol Cell Biol 2011; 12:439 - 52; http://dx.doi.org/10.1038/nrm3143; PMID: 21697901
   PubMed Web of Science ®Google Scholar
• Pérez-Mancera PA, Rust AG, van der Weyden L, Kristiansen G, Li A, Sarver AL, et al, Australian Pancreatic Cancer Genome Initiative. The deubiquitinase USP9X suppresses pancreatic ductal adenocarcinoma. Nature 2012; 486:266 - 70; PMID: 22699621
   PubMed Web of Science ®Google Scholar
• Lengauer C, Kinzler KW, Vogelstein B. Genetic instability in colorectal cancers. Nature 1997; 386:623 - 7; http://dx.doi.org/10.1038/386623a0; PMID: 9121588
   PubMed Web of Science ®Google Scholar
• Bunz F, Hwang PM, Torrance C, Waldman T, Zhang Y, Dillehay L, et al. Disruption of p53 in human cancer cells alters the responses to therapeutic agents. J Clin Invest 1999; 104:263 - 9; http://dx.doi.org/10.1172/JCI6863; PMID: 10430607
   PubMed Web of Science ®Google Scholar
• Hwang PM, Bunz F, Yu J, Rago C, Chan TA, Murphy MP, et al. Ferredoxin reductase affects p53-dependent, 5-fluorouracil-induced apoptosis in colorectal cancer cells. Nat Med 2001; 7:1111 - 7; http://dx.doi.org/10.1038/nm1001-1111; PMID: 11590433
   PubMed Web of Science ®Google Scholar
• Kim JS, Crooks H, Foxworth A, Waldman T. Proof-of-principle: oncogenic beta-catenin is a valid molecular target for the development of pharmacological inhibitors. Mol Cancer Ther 2002; 1:1355 - 9; PMID: 12516970
   PubMed Web of Science ®Google Scholar
• Wilsker D, Bunz F. Loss of ataxia telangiectasia mutated- and Rad3-related function potentiates the effects of chemotherapeutic drugs on cancer cell survival. Mol Cancer Ther 2007; 6:1406 - 13; http://dx.doi.org/10.1158/1535-7163.MCT-06-0679; PMID: 17431119
   PubMed Web of Science ®Google Scholar
• Sur S, Pagliarini R, Bunz F, Rago C, Diaz LA Jr., Kinzler KW, et al. A panel of isogenic human cancer cells suggests a therapeutic approach for cancers with inactivated p53. Proc Natl Acad Sci U S A 2009; 106:3964 - 9; http://dx.doi.org/10.1073/pnas.0813333106; PMID: 19225112
   PubMed Web of Science ®Google Scholar
• Sangster-Guity N, Conrad BH, Papadopoulos N, Bunz F. ATR mediates cisplatin resistance in a p53 genotype-specific manner. Oncogene 2011; 30:2526 - 33; http://dx.doi.org/10.1038/onc.2010.624; PMID: 21258400
   PubMed Web of Science ®Google Scholar
• Karakas B, Weeraratna AT, Abukhdeir AM, Konishi H, Gustin JP, Vitolo MI, et al. P21 gene knock down does not identify genetic effectors seen with gene knock out. Cancer Biol Ther 2007; 6:1025 - 30; http://dx.doi.org/10.4161/cbt.6.7.4202; PMID: 17611398
   PubMed Web of Science ®Google Scholar
• Rajagopalan H, Jallepalli PV, Rago C, Velculescu VE, Kinzler KW, Vogelstein B, et al. Inactivation of hCDC4 can cause chromosomal instability. Nature 2004; 428:77 - 81; http://dx.doi.org/10.1038/nature02313; PMID: 14999283
   PubMed Web of Science ®Google Scholar
• Papageorgis P, Cheng K, Ozturk S, Gong Y, Lambert AW, Abdolmaleky HM, et al. Smad4 inactivation promotes malignancy and drug resistance of colon cancer. Cancer Res 2011; 71:998 - 1008; http://dx.doi.org/10.1158/0008-5472.CAN-09-3269; PMID: 21245094
   PubMed Web of Science ®Google Scholar
• Conroy T, Desseigne F, Ychou M, Bouché O, Guimbaud R, Bécouarn Y, et al, Groupe Tumeurs Digestives of Unicancer, PRODIGE Intergroup. FOLFIRINOX versus gemcitabine for metastatic pancreatic cancer. N Engl J Med 2011; 364:1817 - 25; http://dx.doi.org/10.1056/NEJMoa1011923; PMID: 21561347
   PubMed Web of Science ®Google Scholar
• Topaloglu O, Hurley PJ, Yildirim O, Civin CI, Bunz F. Improved methods for the generation of human gene knockout and knockin cell lines. Nucleic Acids Res 2005; 33:e158; http://dx.doi.org/10.1093/nar/gni160; PMID: 16214806
   PubMed Web of Science ®Google Scholar
• Rago C, Vogelstein B, Bunz F. Genetic knockouts and knockins in human somatic cells. Nat Protoc 2007; 2:2734 - 46; http://dx.doi.org/10.1038/nprot.2007.408; PMID: 18007609
   PubMed Web of Science ®Google Scholar