Novel peptide binds EWS-FLI1 and reduces the oncogenic potential in Ewing tumors

References

• Grier HE, Krailo MD, Tarbell NJ, Link MP, Fryer CJ, Pritchard DJ, et al. Addition of ifosfamide and etoposide to standard chemotherapy for Ewing's sarcoma and primitive neuroectodermal tumor of bone. N Engl J Med 2003; 348:694 - 701; PMID: 12594313; http://dx.doi.org/10.1056/NEJMoa020890
   PubMed Web of Science ®Google Scholar
• Üren A, Toretsky JA. Ewing's sarcoma oncoprotein EWS-FLI1: the perfect target without a therapeutic agent. Future Oncol 2005; 1:521 - 528; PMID: 16556028; http://dx.doi.org/10.2217/14796694.1.4.521
   PubMedGoogle Scholar
• Delattre O, Zucman J, Melot T, Garau XS, Zucker JM, Lenoir GM, et al. The Ewing family of tumors—a subgroup of small-round-cell tumors defined by specific chimeric transcripts. N Engl J Med 1994; 331:294 - 299; PMID: 8022439; http://dx.doi.org/10.1056/NEJM199408043310503
   PubMed Web of Science ®Google Scholar
• Le Deley MC, Delattre O, Schaefer KL, Burchill SA, Koehler G, Hogendoorn PC, et al. Impact of EWS-ETS fusion type on disease progression in Ewing's sarcoma/peripheral primitive neuroectodermal tumor: prospective results from the cooperative Euro-E.W.I.N.G. 99 trial. J Clin Oncol 2010; 28:1982 - 1988; PMID: 20308673; http://dx.doi.org/10.1200/JCO.2009.23.3585
   PubMed Web of Science ®Google Scholar
• Kovar H. Downstream EWS/FLI1—upstream Ewing's sarcoma. Genome Med 2010; 2:8; PMID: 20156317; http://dx.doi.org/10.1186/gm129
   PubMed Web of Science ®Google Scholar
• Burdach S, Plehm S, Unland R, Dirksen U, Borkhardt A, Staege MS, et al. Epigenetic maintenance of stemness and malignancy in peripheral neuroectodermal tumors by EZH2. Cell Cycle 2009; 8:1991 - 1996; PMID: 19502792; http://dx.doi.org/10.4161/cc.8.13.8929
   PubMed Web of Science ®Google Scholar
• Erkizan HV, Uversky VN, Toretsky JA. Oncogenic partnerships: EWS-FLI1 protein interactions initiate key pathways of Ewing's sarcoma. Clin Cancer Res 2010; 16:4077 - 4083; PMID: 20547696; http://dx.doi.org/10.1158/1078-0432.CCR-09-2261
   PubMed Web of Science ®Google Scholar
• Toretsky JA, Erkizan V, Levenson A, Abaan OD, Parvin JD, Cripe TP, et al. Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A. Cancer Res 2006; 66:5574 - 5581; PMID: 16740692; http://dx.doi.org/10.1158/0008-5472.CAN-05-3293
   PubMed Web of Science ®Google Scholar
• Petermann R, Mossier BM, Aryee DN, Khazak V, Golemis EA, Kovar H. Oncogenic EWS-Fli1 interacts with hsRPB7, a subunit of human RNA polymerase II. Oncogene 1998; 17:603 - 610; PMID: 9704926; http://dx.doi.org/10.1038/sj.onc.1201964
   PubMed Web of Science ®Google Scholar
• Spahn L, Petermann R, Siligan C, Schmid JA, Aryee DN, Kovar H. Interaction of the EWS NH2 terminus with BARD1 links the Ewing's sarcoma gene to a common tumor suppressor pathway. Cancer Res 2002; 62:4583 - 4587; PMID: 12183411
   PubMed Web of Science ®Google Scholar
• Nakatani F, Tanaka K, Sakimura R, Matsumoto Y, Matsunobu T, Li X, et al. Identification of p21WAF1/CIP1 as a direct target of EWS-Fli1 oncogenic fusion protein. J Biol Chem 2003; 278:15105 - 15115; PMID: 12560328; http://dx.doi.org/10.1074/jbc.M211470200
   PubMed Web of Science ®Google Scholar
• Kim S, Denny CT, Wisdom R. Cooperative DNA binding with AP-1 proteins is required for transformation by EWS-Ets fusion proteins. Mol Cell Biol 2006; 26:2467 - 2478; PMID: 16537893; http://dx.doi.org/10.1128/MCB.26.7.2467-2478.2006
   PubMed Web of Science ®Google Scholar
• Erkizan HV, Kong Y, Merchant M, Schlottmann S, Barber-Rotenberg JS, Yuan L, et al. A small molecule blocking oncogenic protein EWS-FLI1 interaction with RNA helicase A inhibits growth of Ewing's sarcoma. Nat Med 2009; 15:750 - 756; PMID: 19584866; http://dx.doi.org/10.1038/nm.1983
   PubMed Web of Science ®Google Scholar
• Metallo SJ. Intrinsically disordered proteins are potential drug targets. Curr Opin Chem Biol 2010; 14:481 - 488; PMID: 20598937; http://dx.doi.org/10.1016/j.cbpa.2010.06.169
   PubMed Web of Science ®Google Scholar
• Üren A, Tcherkasskaya O, Toretsky JA. Recombinant EWS-FLI1 oncoprotein activates transcription. Biochemistry 2004; 43:13579 - 13589; PMID: 15491164; http://dx.doi.org/10.1021/bi048776q
   PubMed Web of Science ®Google Scholar
• Ng KP, Potikyan G, Savene RO, Denny CT, Uversky VN, Lee KA. Multiple aromatic side chains within a disordered structure are critical for transcription and transforming activity of EWS family oncoproteins. Proc Natl Acad Sci USA 2007; 104:479 - 484; PMID: 17202261; http://dx.doi.org/10.1073/pnas.0607007104
   PubMed Web of Science ®Google Scholar
• Plescia J, Salz W, Xia F, Pennati M, Zaffaroni N, Daidone MG, et al. Rational design of shepherdin, a novel anticancer agent. Cancer Cell 2005; 7:457 - 468; PMID: 15894266; http://dx.doi.org/10.1016/j.ccr.2005.03.035
   PubMed Web of Science ®Google Scholar
• Mäe M, Langel U. Cell-penetrating peptides as vectors for peptide, protein and oligonucleotide delivery. Curr Opin Pharmacol 2006; 6:509 - 514; PMID: 16860608; http://dx.doi.org/10.1016/j.coph.2006.04.004
   PubMed Web of Science ®Google Scholar
• Zwerner JP, Joo J, Warner KL, Christensen L, Hu-Lieskovan S, Triche TJ, et al. The EWS/FLI1 oncogenic transcription factor deregulates GLI1. Oncogene 2008; 27:3282 - 3291; PMID: 18084326; http://dx.doi.org/10.1038/sj.onc.1210991
   PubMed Web of Science ®Google Scholar
• Beauchamp E, Bulut G, Abaan O, Chen K, Merchant A, Matsui W, et al. GLI1 is a direct transcriptional target of EWS-FLI1 oncoprotein. J Biol Chem 2009; 284:9074 - 9082; PMID: 19189974; http://dx.doi.org/10.1074/jbc.M806233200
   PubMed Web of Science ®Google Scholar
• Khan J, Wei JS, Ringner M, Saal LH, Ladanyi M, Westermann F, et al. Classification and diagnostic prediction of cancers using gene expression profiling and artificial neural networks. Nat Med 2001; 7:673 - 679; PMID: 11385503; http://dx.doi.org/10.1038/89044
   PubMed Web of Science ®Google Scholar
• Knoop LL, Baker SJ. The splicing factor U1C represses EWS/FLI-mediated transactivation. J Biol Chem 2000; 275:24865 - 24871; PMID: 10827180; http://dx.doi.org/10.1074/jbc.M001661200
   PubMed Web of Science ®Google Scholar
• Fuxreiter M, Simon I, Bondos S. Dynamic protein-DNA recognition: beyond what can be seen. Trends Biochem Sci 2011; In press
   PubMed Web of Science ®Google Scholar
• Pande J, Szewczyk MM, Grover AK. Phage display: concept, innovations, applications and future. Biotechnol Adv 2010; 28:849 - 858; PMID: 20659548; http://dx.doi.org/10.1016/j.biotechadv.2010.07.004
   PubMed Web of Science ®Google Scholar
• He J, Li J, Ye C, Zhou L, Zhu J, Wang J, et al. Cell cycle suspension: A novel process lurking in G2 arrest. Cell Cycle 2011; 10:1468 - 1476; PMID: 21455017; http://dx.doi.org/10.4161/cc.10.9.15510
   PubMed Web of Science ®Google Scholar
• Matsumoto Y, Tanaka K, Nakatani F, Matsunobu T, Matsuda S, Iwamoto Y. Downregulation and forced expression of EWS-Fli1 fusion gene results in changes in the expression of G(1)regulatory genes. Br J Cancer 2001; 84:768 - 775; PMID: 11259090; http://dx.doi.org/10.1054/bjoc.2000.1652
   PubMed Web of Science ®Google Scholar
• Hu HM, Zielinska-Kwiatkowska A, Munro K, Wilcox J, Wu DY, Yang L, et al. EWS/FLI1 suppresses retinoblastoma protein function and senescence in Ewing's sarcoma cells. J Orthop Res 2008; 26:886 - 893; PMID: 18271016; http://dx.doi.org/10.1002/jor.20597
   PubMed Web of Science ®Google Scholar
• Kauer M, Ban J, Kofler R, Walker B, Davis S, Meltzer P, et al. A molecular function map of Ewing's sarcoma. PLoS ONE 2009; 4:5415; PMID: 19404404; http://dx.doi.org/10.1371/journal.pone.0005415
   PubMed Web of Science ®Google Scholar
• Khati M. The future of aptamers in medicine. J Clin Pathol 2010; 63:480 - 487; PMID: 20360137; http://dx.doi.org/10.1136/jcp.2008.062786
   PubMed Web of Science ®Google Scholar
• Taraboletti G, Rusnati M, Ragona L, Colombo G. Targeting tumor angiogenesis with TSP-1-based compounds: rational design of antiangiogenic mimetics of endogenous inhibitors. Oncotarget 2010; 1:662 - 673; PMID: 21317461
   PubMed Web of Science ®Google Scholar
• Verdine GL, Walensky LD. The challenge of drugging undruggable targets in cancer: lessons learned from targeting BCL-2 family members. Clin Cancer Res 2007; 13:7264 - 7270; PMID: 18094406; http://dx.doi.org/10.1158/1078-0432.CCR-07-2184
   PubMed Web of Science ®Google Scholar
• Joseph TL, Lane D, Verma CS. Stapled peptides in the p53 pathway: computer simulations reveal novel interactions of the staples with the target protein. Cell Cycle 2010; 9:4560 - 4568; PMID: 21088491; http://dx.doi.org/10.4161/cc.9.22.13816
   PubMed Web of Science ®Google Scholar
• Anderson PM, Tomaras M, McConnell K. Mifamurtide in osteosarcoma—a practical review. Drugs Today (Barc) 2010; 46:327 - 337; PMID: 20517534
   PubMed Web of Science ®Google Scholar
• Borghouts C, Tittmann H, Delis N, Kirchenbauer M, Brill B, Groner B. The intracellular delivery of a recombinant peptide derived from the acidic domain of PIAS3 inhibits STAT3 transactivation and induces tumor cell death. Mol Cancer Res 2010; 8:539 - 553; PMID: 20371673; http://dx.doi.org/10.1158/1541-7786.MCR-09-0417
   PubMed Web of Science ®Google Scholar
• Yamabhai M, Kay BK. Examining the specificity of Src homology 3 domain—ligand interactions with alkaline phosphatase fusion proteins. Anal Biochem 1997; 247:143 - 151; PMID: 9126384; http://dx.doi.org/10.1006/abio.1997.2040
   PubMed Web of Science ®Google Scholar
• Toretsky JA, Kalebic T, Blakesley V, LeRoith D, Helman LJ. The insulin-like growth factor-I receptor is required for EWS/FLI-1 transformation of fibroblasts. J Biol Chem 1997; 272:30822 - 30827; PMID: 9388225; http://dx.doi.org/10.1074/jbc.272.49.30822
   PubMed Web of Science ®Google Scholar
• Voss SD, DeGrand AM, Romeo GR, Cantley LC, Frangioni JV. An integrated vector system for cellular studies of phage display-derived peptides. Anal Biochem 2002; 308:364 - 372; PMID: 12419351; http://dx.doi.org/10.1016/S0003-2697(02)00268-3
   PubMed Web of Science ®Google Scholar
• Beauchamp EM, Ringer L, Bulut G, Sajwan KP, Hall MD, Lee YC, et al. Arsenic trioxide inhibits human cancer cell growth and tumor development in mice by blocking Hedgehog/GLI pathway. J Clin Invest 2011; 121:148 - 160; PMID: 21183792; http://dx.doi.org/10.1172/JCI42874
   PubMed Web of Science ®Google Scholar