p16/p53 expression and telomerase activity in immortalized human dental pulp cells

References

• Gronthos S, Brahim J, Li W, Fisher LW, Cherman N, Boyde A, et al. Stem Cell Properties of Human Dental Pulp Stem Cells. J Dent Res 2002; 81:531 - 535; PMID: 12147742; http://dx.doi.org/10.1177/154405910208100806
   PubMed Web of Science ®Google Scholar
• Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and invivo. Proc Natl Acad Sci USA 2000; 97:13625 - 13630; PMID: 11087820; http://dx.doi.org/10.1073/pnas.240309797
   PubMed Web of Science ®Google Scholar
• Karaöz E, Do an B, Aksoy A, Gacar G, Akyüz S, Ayhan S, et al. Isolation and in vitro characterisation of dental pulp stem cells from natal teeth. Histochem Cell Biol 2010; 133:95 - 112; PMID: 19816704; http://dx.doi.org/10.1007/s00418-009-0646-5
   PubMed Web of Science ®Google Scholar
• Thesleff I, Aberg T. Molecular regulation of tooth development. Bone 1999; 25:123 - 125; PMID: 10423036; http://dx.doi.org/10.1016/S8756-3282(99)00119-2
   PubMed Web of Science ®Google Scholar
• Chen S, Gluhak-Heinrich J, Wang YH, Wu YM, Chuang HH, Chen L, et al. Runx2, Osx and Dspp in Tooth Development. J Dent Res 2009; 88:904 - 909; PMID: 19783797; http://dx.doi.org/10.1177/0022034509342873
   PubMed Web of Science ®Google Scholar
• Tziafas D, Kodonas K. Differentiation Potential of Dental Papilla, Dental Pulp and Apical Papilla Progenitor Cells. J Endod 2010; 36:781 - 789; PMID: 20416419; http://dx.doi.org/10.1016/j.joen.2010.02.006
   PubMed Web of Science ®Google Scholar
• Tziafas D, Smith AJ, Lesot H. Designing new treatment strategies in vital pulp therapy. J Dent 2000; 28:77 - 92; PMID: 10666965; http://dx.doi.org/10.1016/S0300-5712(99)00047-0
   PubMed Web of Science ®Google Scholar
• Yamamura T. Differentiation of pulpal cells and inductive influences of various matrices with reference to pulpal wound-healing. J Dent Res 1985; 64:530 - 540; PMID: 3857253
   PubMed Web of Science ®Google Scholar
• Bender IB. Pulpal Pain Diagnosis—A Review. J Endod 2000; 26:175 - 179; PMID: 11199715; http://dx.doi.org/10.1097/00004770-200003000-00012
   PubMed Web of Science ®Google Scholar
• Jo YY, Lee HJ, Kook SY, Choung HW, Park JY, Chung JH, et al. Isolation and characterization of postnatal stem cells from human dental tissues. Tissue Eng 2007; 13:767 - 773; PMID: 17432951; http://dx.doi.org/10.1089/ten.2006.0192
   PubMedGoogle Scholar
• Mesgouez C, Oboeuf M, Mauro N, Colon P, MacDougall M, Machtou P, et al. Ultrastructural and immunocytochemical characterization of immortalized odontoblast MO6-G3. Int Endod J 2006; 39:453 - 463; PMID: 16674740; http://dx.doi.org/10.1111/j.1365-2591.2006.01089.x
   PubMed Web of Science ®Google Scholar
• Huang AHC, Chen YK, Lin LM, Shieh TY, Chan AWS. Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med 2008; 37:571 - 574; PMID: 18331285; http://dx.doi.org/10.1111/j.1600-0714.2008.00654.x
   PubMed Web of Science ®Google Scholar
• Huang AHC, Chen YK, Chan AWS, Shieh TY, Lin LM. Isolation and Characterization of Human Dental Pulp Stem/Stromal Cells From Nonextracted Crown-fractured Teeth Requiring Root Canal Therapy. J Endod 2009; 35:673 - 681; PMID: 19410081; http://dx.doi.org/10.1016/j.joen.2009.01.019
   PubMed Web of Science ®Google Scholar
• Fujita T, Otsuka-Tanaka Y, Tahara H, Ide T, Abiko Y, Mega Ji. Establishment of immortalized clonal cells derived from periodontal ligament cells by induction of the hTERT gene. J Oral Sci 2005; 47:177 - 184; PMID: 16415561; http://dx.doi.org/10.2334/josnusd.47.177
   PubMedGoogle Scholar
• Dirac AMG, Bernards R. Reversal of Senescence in Mouse Fibroblasts through Lentiviral Suppression of p53. J Biol Chem 2003; 278:11731 - 11734; PMID: 12551891; http://dx.doi.org/10.1074/jbc.C300023200
   PubMed Web of Science ®Google Scholar
• Ohtani N, Yamakoshi K, Takahashi A, Hara E. Realtime in vivo imaging of p16Ink4a gene expression: a new approach to study senescence stress signaling in living animals. Cell Div 2010; 5:1; PMID: 20157424; http://dx.doi.org/10.1186/1747-1028-5-1
   PubMed Web of Science ®Google Scholar
• Gil J, Peters G. Regulation of the INK4b-ARF-INK4a tumour suppressor locus: all for one or one for all. Nat Rev Mol Cell Biol 2006; 7:667 - 677; PMID: 16921403; http://dx.doi.org/10.1038/nrm1987
   PubMed Web of Science ®Google Scholar
• Beauséjour CM, Krtolica A, Galimi F, Narita M, Lowe SW, Yaswen P, et al. Reversal of human cellular senescence: roles of the p53 and p16 pathways. EMBO J 2003; 22:4212 - 4222; PMID: 12912919; http://dx.doi.org/10.1093/emboj/cdg417
   PubMed Web of Science ®Google Scholar
• Chapman EJ, Hurst CD, Pitt E, Chambers P, Aveyard JS, Knowles MA. Expression of hTERT immortalises normal human urothelial cells without inactivation of the p16/Rb pathway. Oncogene 2006; 25:5037 - 5045; PMID: 16619045; http://dx.doi.org/10.1038/sj.onc.1209513
   PubMed Web of Science ®Google Scholar
• Bodnar AG, Ouellette M, Frolkis M, Holt SE, Chiu CP, Morin GB, et al. Extension of life-span by introduction of telomerase into normal human cells. Science 1998; 279:349 - 352; PMID: 9454332; http://dx.doi.org/10.1126/science.279.5349.349
   PubMed Web of Science ®Google Scholar
• Carney SA, Tahara H, Swartz CD, Risinger JI, He H, Moore AB, et al. Immortalization of Human Uterine Leiomyoma and Myometrial Cell Lines After Induction of Telomerase Activity: Molecular and Phenotypic Characteristics. Lab Invest 2002; 82:719 - 728; PMID: 12065682
   PubMed Web of Science ®Google Scholar
• Greider CW. Telomerase RNA levels limit the telomere length equilibrium. Cold Spring Harb Symp Quant Biol 2006; 71:225 - 229; PMID: 17381301; http://dx.doi.org/10.1101/sqb.2006.71.063
   PubMedGoogle Scholar
• Galler KM, Schweikl H, Thonemann B, D'Souza RN, Schmalz G. Human pulp-derived cells immortalized with Simian Virus 40 T-antigen. Eur J Oral Sci 2006; 114:138 - 146; PMID: 16630306; http://dx.doi.org/10.1111/j.1600-0722.2006.00327.x
   PubMed Web of Science ®Google Scholar
• Kamata N, Fujimoto R, Tomonari M, Taki M, Nagayama M, Yasumoto S. Immortalization of human dental papilla, dental pulp, periodontal ligament cells and gingival fibroblasts by telomerase reverse transcriptase. J Oral Pathol Med 2004; 33:417 - 423; PMID: 15250834; http://dx.doi.org/10.1111/j.1600-0714.2004.00228.x
   PubMed Web of Science ®Google Scholar
• Böker W, Yin Z, Drosse I, Haasters F, Rossmann O, Wierer M, et al. Introducing a single-cell-derived human mesenchymal stem cell line expressing hTERT after lentiviral gene transfer. J Cell Mol Med 2008; 12:1347 - 1359; PMID: 18318690; http://dx.doi.org/10.1111/j.1582-4934.2008.00299.x
   PubMed Web of Science ®Google Scholar
• Shay JW, Pereira-Smith OM, Wright WE. A role for both RB and p53 in the regulation of human cellular senescence. Exp Cell Res 1991; 196:33 - 39; PMID: 1652450; http://dx.doi.org/10.1016/0014-4827(91)90453?2
   PubMed Web of Science ®Google Scholar
• Harvey DM, Levine AJ. p53 alteration is a common event in the spontaneous immortalization of primary balb/c murine embryo fibroblasts. Genes Dev 1991; 5:2375 - 2385; PMID: 1752433; http://dx.doi.org/10.1101/gad.5.12b.2375
   PubMed Web of Science ®Google Scholar
• Dickson MA, Hahn WC, Ino Y, Ronfard V, Wu JY, Weinberg RA, et al. Human Keratinocytes That Express hTERT and Also Bypass a p16INK4a-Enforced Mechanism That Limits Lifespan Become Immortal yet Retain Normal Growth and Differentiation Characteristics. Mol Cell Biol 2000; 20:1436 - 1447; PMID: 10648628; http://dx.doi.org/10.1128/MCB.20.4.1436-47.2000
   PubMed Web of Science ®Google Scholar
• Kiyono T, Foster SA, Koop JI, McDougall JK, Galloway DA, Klingelhutz AJ. Both Rb/p16INK4a inactivation and telomerase activity are required to immortalize human epithelial cells. Nature 1998; 396:84 - 88; PMID: 9817205; http://dx.doi.org/10.1038/23962
   PubMed Web of Science ®Google Scholar
• Kitagawa M, Ueda H, Iizuka S, Sakamoto K, Oka H, Kudo Y, et al. Immortalization and characterization of human dental pulp cells with odontoblastic differentiation. Arch Oral Biol 2007; 52:727 - 731; PMID: 17350587; http://dx.doi.org/10.1016/j.archoralbio.2007.02.006
   PubMed Web of Science ®Google Scholar
• Huang GTJ, Sonoyama W, Chen J, Park SH. In vitro characterization of human dental pulp cells: various isolation methods and culturing environments. Cell Tissue Res 2006; 324:225 - 236; PMID: 16440193; http://dx.doi.org/10.1007/s00441-005-0117-9
   PubMed Web of Science ®Google Scholar
• Counter CM, Hahn WC, Wei W, Caddle SD, Beijersbergen RL, Lansdorp PM, et al. Dissociation among in vitro telomerase activity, telomere maintenance and cellular immortalization. Proc Natl Acad Sci USA 1998; 95:14723 - 14728; PMID: 9843956; http://dx.doi.org/10.1073/pnas.95.25.14723
   PubMed Web of Science ®Google Scholar
• Kang MK, Kameta A, Shin KH, Baluda MA, Park N-H. Senescence occurs with hTERT repression and limited telomere shortening in human oral keratinocytes cultured with feeder cells. J Cell Physiol 2004; 199:364 - 370; PMID: 15095283; http://dx.doi.org/10.1002/jcp.10410
   PubMed Web of Science ®Google Scholar
• Tao Q, Lv B, Qiao B, Zheng Cq, Chen Zf. Immortalization of ameloblastoma cells via reactivation of telomerase function: Phenotypic and molecular characteristics. Oral Oncol 2009; 45:239 - 244; PMID: 19833545; http://dx.doi.org/10.1016/j.oraloncology.2009.08.007
   PubMed Web of Science ®Google Scholar
• Liu X, Dakic A, Chen RX, Disbrow GL, Zhang YY, Dai YH, et al. Cell-Restricted Immortalization by Human Papillomavirus Correlates with Telomerase Activation and Engagement of the hTERT Promoter by Myc. J Virol 2008; 82:11568 - 11576; PMID: 18818322; http://dx.doi.org/10.1128/JVI.01318-08
   PubMed Web of Science ®Google Scholar
• Haferkamp S, Tran SL, Becker TM, Scurr LL, Kefford RF, Rizos H. The relative contributions of the p53 and pRb pathways in oncogene induced melanocyte senescence. Aging (Albany, NY) 2009; 1:542 - 556; PMID: 20157537
   PubMedGoogle Scholar
• Wright WE, Shay JW. Telomere dynamics in cancer progression and prevention: fundamental differences in human and mouse telomere biology. Nat Med 2000; 6:849 - 851; PMID: 10932210; http://dx.doi.org/10.1038/78592
   PubMed Web of Science ®Google Scholar
• Chimenti C, Kajstura J, Torella D, Urbanek K, Heleniak H, Colussi C, et al. Senescence and death of primitive cells and myocytes lead to premature cardiac aging and heart failure. Circ Res 2003; 93:604 - 613; PMID: 12958145; http://dx.doi.org/10.1161/01.RES.0000093985.76901.AF
   PubMed Web of Science ®Google Scholar
• Torella D, Rota M, Nurzynska D, Musso E, Monsen A, Shiraishi I, et al. Cardiac stem cell and myocyte aging, heart failure and insulin-like growth factor-1 overexpression. Circ Res 2004; 94:514 - 524; PMID: 14726476; http://dx.doi.org/10.1161/01.RES.0000117306.10142.50
   PubMed Web of Science ®Google Scholar
• Migliaccio M, Amacker M, Just T, Reichenbach P, Valmori D, Cerottini JC, et al. Ectopic Human Telomerase Catalytic Subunit Expression Maintains Telomere Length But Is Not Sufficient for CD8+ T Lymphocyte Immortalization. J Immunol 2000; 165:4978 - 4984; PMID: 11046025
   PubMed Web of Science ®Google Scholar
• de Jonge HJM, Woolthuis CM, de Bont E, Huls G. Paradoxical downregulation of p16(INK4a) mRNA with advancing age in Acute Myeloid Leukemia. Aging (Albany, NY) 2009; 1:949 - 953
   PubMedGoogle Scholar
• Counter CM, Hahn WC, Wei WY, Caddle SD, Beijersbergen RL, Lansdorp PM, et al. Dissociation among in vitro telomerase activity, telomere maintenance and cellular immortalization. Proc Natl Acad Sci USA 1998; 95:14723 - 14728; PMID: 9843956; http://dx.doi.org/10.1073/pnas.95.25.14723
   PubMed Web of Science ®Google Scholar
• Oreffo ROC, Driessens FCM, Planell JA, Triffitt JT. Growth and differentiation of human bone marrow osteoprogenitors on novel calcium phosphate cements. Biomaterials 1998; 19:1845 - 1854; PMID: 9855185; http://dx.doi.org/10.1016/S0142-9612(98)00084-2
   PubMed Web of Science ®Google Scholar
• Rao J, Otto WR. Fluorimetric DNA assay for cell growth estimation. Anal Biochem 1992; 207:186 - 192; PMID: 1489093; http://dx.doi.org/10.1016/0003-2697(92)90521?8
   PubMed Web of Science ®Google Scholar