Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis

References

• Bjornsti MA, Houghton PJ. The TOR pathway: a target for cancer therapy. Nat Rev Cancer 2004; 4:335 - 48; http://dx.doi.org/10.1038/nrc1362; PMID: 15122205
   PubMed Web of Science ®Google Scholar
• Wullschleger S, Loewith R, Hall MN. TOR signaling in growth and metabolism. Cell 2006; 124:471 - 84; http://dx.doi.org/10.1016/j.cell.2006.01.016; PMID: 16469695
   PubMed Web of Science ®Google Scholar
• Sabatini DM. mTOR and cancer: insights into a complex relationship. Nat Rev Cancer 2006; 6:729 - 34; http://dx.doi.org/10.1038/nrc1974; PMID: 16915295
   PubMed Web of Science ®Google Scholar
• Guertin DA, Sabatini DM. Defining the role of mTOR in cancer. Cancer Cell 2007; 12:9 - 22; http://dx.doi.org/10.1016/j.ccr.2007.05.008; PMID: 17613433
   PubMed Web of Science ®Google Scholar
• Bhaskar PT, Hay N. The two TORCs and Akt. Dev Cell 2007; 12:487 - 502; http://dx.doi.org/10.1016/j.devcel.2007.03.020; PMID: 17419990
   PubMed Web of Science ®Google Scholar
• Hall MN. mTOR-what does it do?. Transplant Proc 2008; 40:Suppl S5 - 8; http://dx.doi.org/10.1016/j.transproceed.2008.10.009; PMID: 19100909
   PubMedGoogle Scholar
• Peterson RT, Beal PA, Comb MJ, Schreiber SL. FKBP12-rapamycin-associated protein (FRAP) autophosphorylates at serine 2481 under translationally repressive conditions. J Biol Chem 2000; 275:7416 - 23; http://dx.doi.org/10.1074/jbc.275.10.7416; PMID: 10702316
   PubMed Web of Science ®Google Scholar
• Chiang GG, Abraham RT. Phosphorylation of mammalian target of rapamycin (mTOR) at Ser-2448 is mediated by p70S6 kinase. J Biol Chem 2005; 280:25485 - 90; http://dx.doi.org/10.1074/jbc.M501707200; PMID: 15899889
   PubMed Web of Science ®Google Scholar
• Holz MK, Blenis J. Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase. J Biol Chem 2005; 280:26089 - 93; http://dx.doi.org/10.1074/jbc.M504045200; PMID: 15905173
   PubMed Web of Science ®Google Scholar
• Copp J, Manning G, Hunter T. TORC-specific phosphorylation of mammalian target of rapamycin (mTOR): phospho-Ser2481 is a marker for intact mTOR signaling complex 2. Cancer Res 2009; 69:1821 - 7; http://dx.doi.org/10.1158/0008-5472.CAN-08-3014; PMID: 19244117
   PubMed Web of Science ®Google Scholar
• Sarbassov DD, Ali SM, Sengupta S, Sheen JH, Hsu PP, Bagley AF, et al. Prolonged rapamycin treatment inhibits mTORC2 assembly and Akt/PKB. Mol Cell 2006; 22:159 - 68; http://dx.doi.org/10.1016/j.molcel.2006.03.029; PMID: 16603397
   PubMed Web of Science ®Google Scholar
• Soliman GA, Acosta-Jaquez HA, Dunlop EA, Ekim B, Maj NE, Tee AR, et al. mTOR Ser-2481 autophosphorylation monitors mTORC-specific catalytic activity and clarifies rapamycin mechanism of action. J Biol Chem 2010; 285:7866 - 79; http://dx.doi.org/10.1074/jbc.M109.096222; PMID: 20022946
   PubMed Web of Science ®Google Scholar
• Yaba A, Bianchi V, Borini A, Johnson J. A putative mitotic checkpoint dependent on mTOR function controls cell proliferation and survival in ovarian granulosa cells. Reprod Sci 2008; 15:128 - 38; http://dx.doi.org/10.1177/1933719107312037; PMID: 18276949
   PubMed Web of Science ®Google Scholar
• Yu J, Yaba A, Kasiman C, Thomson T, Johnson J. mTOR controls ovarian follicle growth by regulating granulosa cell proliferation. PLoS One 2011; 6:e21415; http://dx.doi.org/10.1371/journal.pone.0021415; PMID: 21750711
   PubMed Web of Science ®Google Scholar
• Doghman M, El Wakil A, Cardinaud B, Thomas E, Wang J, Zhao W, et al. Regulation of insulin-like growth factor-mammalian target of rapamycin signaling by microRNA in childhood adrenocortical tumors. Cancer Res 2010; 70:4666 - 75; http://dx.doi.org/10.1158/0008-5472.CAN-09-3970; PMID: 20484036
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Oliveras-Ferraros C, Bernadó L, López-Bonet E, Menendez JA. The serine 2481-autophosphorylated form of mammalian Target Of Rapamycin (mTOR) is localized to midzone and midbody in dividing cancer cells. Biochem Biophys Res Commun 2009; 380:638 - 43; http://dx.doi.org/10.1016/j.bbrc.2009.01.153; PMID: 19285014
   PubMed Web of Science ®Google Scholar
• Lopez-Bonet E, Vazquez-Martin A, Pérez-Martínez MC, Oliveras-Ferraros C, Pérez-Bueno F, Bernadó L, et al. Serine 2481-autophosphorylation of mammalian target of rapamycin (mTOR) couples with chromosome condensation and segregation during mitosis: confocal microscopy characterization and immunohistochemical validation of PP-mTOR(Ser2481) as a novel high-contrast mitosis marker in breast cancer core biopsies. Int J Oncol 2010; 36:107 - 15; PMID: 19956839
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, López-Bonet E, Oliveras-Ferraros C, Pérez-Martínez MC, Bernadó L, Menendez JA. Mitotic kinase dynamics of the active form of AMPK (phospho-AMPKalphaThr172) in human cancer cells. Cell Cycle 2009; 8:788 - 91; http://dx.doi.org/10.4161/cc.8.5.7787; PMID: 19221486
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Oliveras-Ferraros C, Menendez JA. The active form of the metabolic sensor: AMP-activated protein kinase (AMPK) directly binds the mitotic apparatus and travels from centrosomes to the spindle midzone during mitosis and cytokinesis. Cell Cycle 2009; 8:2385 - 98; http://dx.doi.org/10.4161/cc.8.15.9082; PMID: 19556893
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Oliveras-Ferraros C, Lopez-Bonet E, Menendez JA. AMPK: Evidence for an energy-sensing cytokinetic tumor suppressor. Cell Cycle 2009; 8:3679 - 83; http://dx.doi.org/10.4161/cc.8.22.9905; PMID: 19844168
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Oliveras-Ferraros C, Cufí S, Menendez JA. Polo-like kinase 1 regulates activation of AMP-activated protein kinase (AMPK) at the mitotic apparatus. Cell Cycle 2011; 10:1295 - 302; http://dx.doi.org/10.4161/cc.10.8.15342; PMID: 21474997
   PubMed Web of Science ®Google Scholar
• Menendez JA, Vazquez-Martin A. AMPK: A bona fide resident of the mitotic spindle midzone. Cell Cycle 2012; 11:841 - 2; http://dx.doi.org/10.4161/cc.11.5.19461; PMID: 22336917
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Cufí S, Oliveras-Ferraros C, Menendez JA. Polo-like kinase 1 directs the AMPK-mediated activation of myosin regulatory light chain at the cytokinetic cleavage furrow independently of energy balance. Cell Cycle 2012; 11:2422 - 6; http://dx.doi.org/10.4161/cc.20438; PMID: 22672936
   PubMed Web of Science ®Google Scholar
• Pinter K, Jefferson A, Czibik G, Watkins H, Redwood C. Subunit composition of AMPK trimers present in the cytokinetic apparatus: Implications for drug target identification. Cell Cycle 2012; 11:917 - 21; http://dx.doi.org/10.4161/cc.11.5.19412; PMID: 22333580
   PubMed Web of Science ®Google Scholar
• Carmena M. A mitosis-specific AMPK trimer?. Cell Cycle 2012; 11:1269; http://dx.doi.org/10.4161/cc.19902; PMID: 22426423
   PubMed Web of Science ®Google Scholar
• Banko MR, Allen JJ, Schaffer BE, Wilker EW, Tsou P, White JL, et al. Chemical genetic screen for AMPKα2 substrates uncovers a network of proteins involved in mitosis. Mol Cell 2011; 44:878 - 92; http://dx.doi.org/10.1016/j.molcel.2011.11.005; PMID: 22137581
   PubMed Web of Science ®Google Scholar
• Robitaille AM, Hall MN. Ramping up mitosis: an AMPKα2-regulated signaling network promotes mitotic progression. Mol Cell 2012; 45:8 - 9; http://dx.doi.org/10.1016/j.molcel.2011.12.018; PMID: 22244327
   PubMed Web of Science ®Google Scholar
• Wrighton KH. AMPK moonlights in mitosis. Nat Rev Mol Cell Biol 2012; 13:64; PMID: 22251904
   PubMed Web of Science ®Google Scholar
• Thaiparambil JT, Eggers CM, Marcus AI. AMPK regulates mitotic spindle orientation through phosphorylation of myosin regulatory light chain. Mol Cell Biol 2012; 32:3203 - 17; http://dx.doi.org/10.1128/MCB.00418-12; PMID: 22688514
   PubMed Web of Science ®Google Scholar
• Schmidt T, Wahl P, Wüthrich RP, Vogetseder A, Picard N, Kaissling B, et al. Immunolocalization of phospho-S6 kinases: a new way to detect mitosis in tissue sections and in cell culture. Histochem Cell Biol 2007; 127:123 - 9; http://dx.doi.org/10.1007/s00418-006-0255-5; PMID: 17136413
   PubMed Web of Science ®Google Scholar
• Egervári G, Márk A, Hajdu M, Barna G, Sápi Z, Krenács T, et al. Mitotic lymphoma cells are characterized by high expression of phosphorylated ribosomal S6 protein. Histochem Cell Biol 2011; 135:409 - 17; http://dx.doi.org/10.1007/s00418-011-0803-5; PMID: 21424608
   PubMed Web of Science ®Google Scholar
• Rossi R, Pester JM, McDowell M, Soza S, Montecucco A, Lee-Fruman KK. Identification of S6K2 as a centrosome-located kinase. FEBS Lett 2007; 581:4058 - 64; http://dx.doi.org/10.1016/j.febslet.2007.07.047; PMID: 17678899
   PubMed Web of Science ®Google Scholar
• Gwinn DM, Asara JM, Shaw RJ. Raptor is phosphorylated by cdc2 during mitosis. PLoS One 2010; 5:e9197; http://dx.doi.org/10.1371/journal.pone.0009197; PMID: 20169205
   PubMed Web of Science ®Google Scholar
• Ramírez-Valle F, Badura ML, Braunstein S, Narasimhan M, Schneider RJ. Mitotic raptor promotes mTORC1 activity, G(2)/M cell cycle progression, and internal ribosome entry site-mediated mRNA translation. Mol Cell Biol 2010; 30:3151 - 64; http://dx.doi.org/10.1128/MCB.00322-09; PMID: 20439490
   PubMed Web of Science ®Google Scholar
• Vazquez-Martin A, Cufí S, Oliveras-Ferraros C, Menendez JA. Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 2011; 10:3140 - 52; http://dx.doi.org/10.4161/cc.10.18.17376; PMID: 21900751
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Aging and immortality: quasi-programmed senescence and its pharmacologic inhibition. Cell Cycle 2006; 5:2087 - 102; http://dx.doi.org/10.4161/cc.5.18.3288; PMID: 17012837
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. An anti-aging drug today: from senescence-promoting genes to anti-aging pill. Drug Discov Today 2007; 12:218 - 24; http://dx.doi.org/10.1016/j.drudis.2007.01.004; PMID: 17331886
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Prevention of cancer by inhibiting aging. Cancer Biol Ther 2008; 7:1520 - 4; http://dx.doi.org/10.4161/cbt.7.10.6663; PMID: 18769112
   PubMed Web of Science ®Google Scholar
• de Magalhães JP, Faragher RG. Cell divisions and mammalian aging: integrative biology insights from genes that regulate longevity. Bioessays 2008; 30:567 - 78; http://dx.doi.org/10.1002/bies.20760; PMID: 18478536
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Validation of anti-aging drugs by treating age-related diseases. Aging (Albany NY) 2009; 1:281 - 8; PMID: 20157517
   PubMedGoogle Scholar
• Blagosklonny MV. Aging-suppressants: cellular senescence (hyperactivation) and its pharmacologic deceleration. Cell Cycle 2009; 8:1883 - 7; http://dx.doi.org/10.4161/cc.8.12.8815; PMID: 19448395
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. TOR-driven aging: speeding car without brakes. Cell Cycle 2009; 8:4055 - 9; http://dx.doi.org/10.4161/cc.8.24.10310; PMID: 19923900
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Rapamycin and quasi-programmed aging: four years later. Cell Cycle 2010; 9:1859 - 62; http://dx.doi.org/10.4161/cc.9.10.11872; PMID: 20436272
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Molecular damage in cancer: an argument for mTOR-driven aging. Aging (Albany NY) 2011; 3:1130 - 41; PMID: 22246147
   PubMedGoogle Scholar
• Menendez JA, Cufí S, Oliveras-Ferraros C, Vellon L, Joven J, Vazquez-Martin A. Gerosuppressant metformin: less is more. Aging (Albany NY) 2011; 3:348 - 62; PMID: 21483040
   PubMedGoogle Scholar
• Menendez JA, Vellon L, Oliveras-Ferraros C, Cufí S, Vazquez-Martin A. mTOR-regulated senescence and autophagy during reprogramming of somatic cells to pluripotency: a roadmap from energy metabolism to stem cell renewal and aging. Cell Cycle 2011; 10:3658 - 77; http://dx.doi.org/10.4161/cc.10.21.18128; PMID: 22052357
   PubMed Web of Science ®Google Scholar
• Blagosklonny MV. Cell cycle arrest is not yet senescence, which is not just cell cycle arrest: terminology for TOR-driven aging. Aging (Albany NY) 2012; 4:159 - 65; PMID: 22394614
   PubMedGoogle Scholar