A genome-wide approach reveals novel imprinted genes expressed in the human placenta

References

• Reik W, Walter J. Genomic imprinting: parental influence on the genome. Nat Rev Genet 2001; 2:21 - 32; http://dx.doi.org/10.1038/35047554; PMID: 11253064
   PubMed Web of Science ®Google Scholar
• Varrault A, Gueydan C, Delalbre A, Bellmann A, Houssami S, Aknin C, et al. Zac1 regulates an imprinted gene network critically involved in the control of embryonic growth. Dev Cell 2006; 11:711 - 22; http://dx.doi.org/10.1016/j.devcel.2006.09.003; PMID: 17084362
   PubMed Web of Science ®Google Scholar
• Graves JA. Genomic imprinting, development and disease--is pre-eclampsia caused by a maternally imprinted gene?. Reprod Fertil Dev 1998; 10:23 - 9; http://dx.doi.org/10.1071/R98014; PMID: 9727590
   PubMed Web of Science ®Google Scholar
• Fenstad MH, Johnson MP, Løset M, Mundal SB, Roten LT, Eide IP, et al. STOX2 but not STOX1 is differentially expressed in decidua from pre-eclamptic women: data from the Second Nord-Trondelag Health Study. Mol Hum Reprod 2010; 16:960 - 8; http://dx.doi.org/10.1093/molehr/gaq064; PMID: 20643876
   PubMed Web of Science ®Google Scholar
• Kanayama N, Takahashi K, Matsuura T, Sugimura M, Kobayashi T, Moniwa N, et al. Deficiency in p57Kip2 expression induces preeclampsia-like symptoms in mice. Mol Hum Reprod 2002; 8:1129 - 35; http://dx.doi.org/10.1093/molehr/8.12.1129; PMID: 12468647
   PubMed Web of Science ®Google Scholar
• Rigourd V, Chauvet C, Chelbi ST, Rebourcet R, Mondon F, Letourneur F, et al. STOX1 overexpression in choriocarcinoma cells mimics transcriptional alterations observed in preeclamptic placentas. PLoS One 2008; 3:e3905; http://dx.doi.org/10.1371/journal.pone.0003905; PMID: 19079545
   PubMed Web of Science ®Google Scholar
• van Dijk M, Mulders J, Poutsma A, Könst AA, Lachmeijer AM, Dekker GA, et al. Maternal segregation of the Dutch preeclampsia locus at 10q22 with a new member of the winged helix gene family. Nat Genet 2005; 37:514 - 9; http://dx.doi.org/10.1038/ng1541; PMID: 15806103
   PubMed Web of Science ®Google Scholar
• van Dijk M, van Bezu J, Poutsma A, Veerhuis R, Rozemuller AJ, Scheper W, et al. The pre-eclampsia gene STOX1 controls a conserved pathway in placenta and brain upregulated in late-onset Alzheimer’s disease. J Alzheimers Dis 2010; 19:673 - 9; PMID: 20110611
   PubMed Web of Science ®Google Scholar
• Brideau CM, Eilertson KE, Hagarman JA, Bustamante CD, Soloway PD. Successful computational prediction of novel imprinted genes from epigenomic features. Mol Cell Biol 2010; 30:3357 - 70; http://dx.doi.org/10.1128/MCB.01355-09; PMID: 20421412
   PubMed Web of Science ®Google Scholar
• Hutter B, Bieg M, Helms V, Paulsen M. Imprinted genes show unique patterns of sequence conservation. BMC Genomics 2010; 11:649; http://dx.doi.org/10.1186/1471-2164-11-649; PMID: 21092170
   PubMed Web of Science ®Google Scholar
• Luedi PP, Dietrich FS, Weidman JR, Bosko JM, Jirtle RL, Hartemink AJ. Computational and experimental identification of novel human imprinted genes. Genome Res 2007; 17:1723 - 30; http://dx.doi.org/10.1101/gr.6584707; PMID: 18055845
   PubMed Web of Science ®Google Scholar
• Luedi PP, Hartemink AJ, Jirtle RL. Genome-wide prediction of imprinted murine genes. Genome Res 2005; 15:875 - 84; http://dx.doi.org/10.1101/gr.3303505; PMID: 15930497
   PubMed Web of Science ®Google Scholar
• Pollard KS, Serre D, Wang X, Tao H, Grundberg E, Hudson TJ, et al. A genome-wide approach to identifying novel-imprinted genes. Hum Genet 2008; 122:625 - 34; http://dx.doi.org/10.1007/s00439-007-0440-1; PMID: 17955261
   PubMed Web of Science ®Google Scholar
• Wang Z, Fan H, Yang HH, Hu Y, Buetow KH, Lee MP. Comparative sequence analysis of imprinted genes between human and mouse to reveal imprinting signatures. Genomics 2004; 83:395 - 401; http://dx.doi.org/10.1016/j.ygeno.2003.09.007; PMID: 14962665
   PubMed Web of Science ®Google Scholar
• Yang HH, Hu Y, Edmonson M, Buetow K, Lee MP. Computation method to identify differential allelic gene expression and novel imprinted genes. Bioinformatics 2003; 19:952 - 5; http://dx.doi.org/10.1093/bioinformatics/btg127; PMID: 12761057
   PubMed Web of Science ®Google Scholar
• Choufani S, Shapiro JS, Susiarjo M, Butcher DT, Grafodatskaya D, Lou Y, et al. A novel approach identifies new differentially methylated regions (DMRs) associated with imprinted genes. Genome Res 2011; 21:465 - 76; http://dx.doi.org/10.1101/gr.111922.110; PMID: 21324877
   PubMed Web of Science ®Google Scholar
• Ruf N, Dünzinger U, Brinckmann A, Haaf T, Nürnberg P, Zechner U. Expression profiling of uniparental mouse embryos is inefficient in identifying novel imprinted genes. Genomics 2006; 87:509 - 19; http://dx.doi.org/10.1016/j.ygeno.2005.12.007; PMID: 16455231
   PubMed Web of Science ®Google Scholar
• Smith RJ, Dean W, Konfortova G, Kelsey G. Identification of novel imprinted genes in a genome-wide screen for maternal methylation. Genome Res 2003; 13:558 - 69; http://dx.doi.org/10.1101/gr.781503; PMID: 12670997
   PubMed Web of Science ®Google Scholar
• Sritanaudomchai H, Ma H, Clepper L, Gokhale S, Bogan R, Hennebold J, et al. Discovery of a novel imprinted gene by transcriptional analysis of parthenogenetic embryonic stem cells. Hum Reprod 2010; 25:1927 - 41; http://dx.doi.org/10.1093/humrep/deq144; PMID: 20522441
   PubMed Web of Science ®Google Scholar
• Ruf N, Bähring S, Galetzka D, Pliushch G, Luft FC, Nürnberg P, et al. Sequence-based bioinformatic prediction and QUASEP identify genomic imprinting of the KCNK9 potassium channel gene in mouse and human. Hum Mol Genet 2007; 16:2591 - 9; http://dx.doi.org/10.1093/hmg/ddm216; PMID: 17704508
   PubMed Web of Science ®Google Scholar
• Pant PV, Tao H, Beilharz EJ, Ballinger DG, Cox DR, Frazer KA. Analysis of allelic differential expression in human white blood cells. Genome Res 2006; 16:331 - 9; http://dx.doi.org/10.1101/gr.4559106; PMID: 16467561
   PubMed Web of Science ®Google Scholar
• Yoshida Y, Tsunoda T, Takashima Y, Fujimoto T, Doi K, Sasazuki T, et al. ZFAT is essential for endothelial cell assembly and the branch point formation of capillary-like structures in an angiogenesis model. Cell Mol Biol Lett 2010; 15:541 - 50; http://dx.doi.org/10.2478/s11658-010-0028-y; PMID: 20645017
   PubMed Web of Science ®Google Scholar
• Shirasawa S, Harada H, Furugaki K, Akamizu T, Ishikawa N, Ito K, et al. SNPs in the promoter of a B cell-specific antisense transcript, SAS-ZFAT, determine susceptibility to autoimmune thyroid disease. Hum Mol Genet 2004; 13:2221 - 31; http://dx.doi.org/10.1093/hmg/ddh245; PMID: 15294872
   PubMed Web of Science ®Google Scholar
• Cattanach BM, Kirk M. Differential activity of maternally and paternally derived chromosome regions in mice. Nature 1985; 315:496 - 8; http://dx.doi.org/10.1038/315496a0; PMID: 4000278
   PubMed Web of Science ®Google Scholar
• Kanber D, Berulava T, Ammerpohl O, Mitter D, Richter J, Siebert R, et al. The human retinoblastoma gene is imprinted. PLoS Genet 2009; 5:e1000790; http://dx.doi.org/10.1371/journal.pgen.1000790; PMID: 20041224
   PubMedGoogle Scholar
• Daelemans C, Ritchie ME, Smits G, Abu-Amero S, Sudbery IM, Forrest MS, et al. High-throughput analysis of candidate imprinted genes and allele-specific gene expression in the human term placenta. BMC Genet 2010; 11:25; http://dx.doi.org/10.1186/1471-2156-11-25; PMID: 20403199
   PubMed Web of Science ®Google Scholar
• Gimelbrant A, Hutchinson JN, Thompson BR, Chess A. Widespread monoallelic expression on human autosomes. Science 2007; 318:1136 - 40; http://dx.doi.org/10.1126/science.1148910; PMID: 18006746
   PubMed Web of Science ®Google Scholar
• Blagitko N, Mergenthaler S, Schulz U, Wollmann HA, Craigen W, Eggermann T, et al. Human GRB10 is imprinted and expressed from the paternal and maternal allele in a highly tissue- and isoform-specific fashion. Hum Mol Genet 2000; 9:1587 - 95; http://dx.doi.org/10.1093/hmg/9.11.1587; PMID: 10861285
   PubMed Web of Science ®Google Scholar
• Nakabayashi K, Trujillo AM, Tayama C, Camprubi C, Yoshida W, Lapunzina P, et al. Methylation screening of reciprocal genome-wide UPDs identifies novel human-specific imprinted genes. Hum Mol Genet 2011; 20:3188 - 97; http://dx.doi.org/10.1093/hmg/ddr224; PMID: 21593219
   PubMed Web of Science ®Google Scholar
• Tanabe Y, Hirano A, Iwasato T, Itohara S, Araki K, Yamaguchi T, et al. Molecular characterization and gene disruption of a novel zinc-finger protein, HIT-4, expressed in rodent brain. J Neurochem 2010; 112:1035 - 44; http://dx.doi.org/10.1111/j.1471-4159.2009.06525.x; PMID: 19968752
   PubMed Web of Science ®Google Scholar
• Gil OD, Zanazzi G, Struyk AF, Salzer JL. Neurotrimin mediates bifunctional effects on neurite outgrowth via homophilic and heterophilic interactions. J Neurosci 1998; 18:9312 - 25; PMID: 9801370
   PubMed Web of Science ®Google Scholar
• Marshall CR, Young EJ, Pani AM, Freckmann ML, Lacassie Y, Howald C, et al. Infantile spasms is associated with deletion of the MAGI2 gene on chromosome 7q11.23-q21.11. Am J Hum Genet 2008; 83:106 - 11; http://dx.doi.org/10.1016/j.ajhg.2008.06.001; PMID: 18565486
   PubMed Web of Science ®Google Scholar
• Doi K, Fujimoto T, Koyanagi M, Tsunoda T, Tanaka Y, Yoshida Y, et al. ZFAT is a critical molecule for cell survival in mouse embryonic fibroblasts. Cell Mol Biol Lett 2011; 16:89 - 100; http://dx.doi.org/10.2478/s11658-010-0041-1; PMID: 21225468
   PubMed Web of Science ®Google Scholar
• Fujimoto T, Doi K, Koyanagi M, Tsunoda T, Takashima Y, Yoshida Y, et al. ZFAT is an antiapoptotic molecule and critical for cell survival in MOLT-4 cells. FEBS Lett 2009; 583:568 - 72; http://dx.doi.org/10.1016/j.febslet.2008.12.063; PMID: 19162026
   PubMed Web of Science ®Google Scholar
• Kang HS, Kim YS, ZeRuth G, Beak JY, Gerrish K, Kilic G, et al. Transcription factor Glis3, a novel critical player in the regulation of pancreatic beta-cell development and insulin gene expression. Mol Cell Biol 2009; 29:6366 - 79; http://dx.doi.org/10.1128/MCB.01259-09; PMID: 19805515
   PubMed Web of Science ®Google Scholar
• Yang Y, Chang BH, Yechoor V, Chen W, Li L, Tsai MJ, et al. The Krüppel-like zinc finger protein GLIS3 transactivates neurogenin 3 for proper fetal pancreatic islet differentiation in mice. Diabetologia 2011; 54:2595 - 605; http://dx.doi.org/10.1007/s00125-011-2255-9; PMID: 21786021
   PubMed Web of Science ®Google Scholar
• Cetin I, Antonazzo P. The role of the placenta in intrauterine growth restriction (IUGR). Z Geburtshilfe Neonatol 2009; 213:84 - 8; http://dx.doi.org/10.1055/s-0029-1224143; PMID: 19536707
   PubMedGoogle Scholar
• Jansson T, Myatt L, Powell TL. The role of trophoblast nutrient and ion transporters in the development of pregnancy complications and adult disease. Curr Vasc Pharmacol 2009; 7:521 - 33; http://dx.doi.org/10.2174/157016109789043982; PMID: 19485888
   PubMed Web of Science ®Google Scholar
• King CE, Wang L, Winograd R, Madison BB, Mongroo PS, Johnstone CN, et al. LIN28B fosters colon cancer migration, invasion and transformation through let-7-dependent and -independent mechanisms. Oncogene 2011; 30:4185 - 93; http://dx.doi.org/10.1038/onc.2011.131; PMID: 21625210
   PubMed Web of Science ®Google Scholar
• Zhu H, Shyh-Chang N, Segrè AV, Shinoda G, Shah SP, Einhorn WS, et al, DIAGRAM Consortium, MAGIC Investigators. The Lin28/let-7 axis regulates glucose metabolism. Cell 2011; 147:81 - 94; http://dx.doi.org/10.1016/j.cell.2011.08.033; PMID: 21962509
   PubMed Web of Science ®Google Scholar
• Elks CE, Ong KK. Whole genome associated studies for age at menarche. Brief Funct Genomics 2011; 10:91 - 7; http://dx.doi.org/10.1093/bfgp/elq030; PMID: 21436305
   PubMed Web of Science ®Google Scholar
• Balzer E, Heine C, Jiang Q, Lee VM, Moss EG. LIN28 alters cell fate succession and acts independently of the let-7 microRNA during neurogliogenesis in vitro. Development 2010; 137:891 - 900; http://dx.doi.org/10.1242/dev.042895; PMID: 20179095
   PubMed Web of Science ®Google Scholar
• Polesskaya A, Cuvellier S, Naguibneva I, Duquet A, Moss EG, Harel-Bellan A. Lin-28 binds IGF-2 mRNA and participates in skeletal myogenesis by increasing translation efficiency. Genes Dev 2007; 21:1125 - 38; http://dx.doi.org/10.1101/gad.415007; PMID: 17473174
   PubMed Web of Science ®Google Scholar
• Lu L, Katsaros D, Shaverdashvili K, Qian B, Wu Y, de la Longrais IA, et al. Pluripotent factor lin-28 and its homologue lin-28b in epithelial ovarian cancer and their associations with disease outcomes and expression of let-7a and IGF-II. Eur J Cancer 2009; 45:2212 - 8; http://dx.doi.org/10.1016/j.ejca.2009.05.003; PMID: 19477633
   PubMed Web of Science ®Google Scholar
• Liang J, Wang J, Azfer A, Song W, Tromp G, Kolattukudy PE, et al. A novel CCCH-zinc finger protein family regulates proinflammatory activation of macrophages. J Biol Chem 2008; 283:6337 - 46; http://dx.doi.org/10.1074/jbc.M707861200; PMID: 18178554
   PubMed Web of Science ®Google Scholar
• Lockwood CJ, Huang SJ, Krikun G, Caze R, Rahman M, Buchwalder LF, et al. Decidual hemostasis, inflammation, and angiogenesis in pre-eclampsia. Semin Thromb Hemost 2011; 37:158 - 64; http://dx.doi.org/10.1055/s-0030-1270344; PMID: 21370218
   PubMed Web of Science ®Google Scholar
• Lockwood CJ, Krikun G, Caze R, Rahman M, Buchwalder LF, Schatz F. Decidual cell-expressed tissue factor in human pregnancy and its involvement in hemostasis and preeclampsia-related angiogenesis. Ann N Y Acad Sci 2008; 1127:67 - 72; http://dx.doi.org/10.1196/annals.1434.013; PMID: 18443332
   PubMed Web of Science ®Google Scholar
• Kim HN, Lee EJ, Jung SC, Lee JY, Chung HW, Kim HL. Genetic variants that affect length/height in infancy/early childhood in Vietnamese-Korean families. J Hum Genet 2010; 55:681 - 90; http://dx.doi.org/10.1038/jhg.2010.88; PMID: 20668459
   PubMed Web of Science ®Google Scholar
• Takeuchi F, Nabika T, Isono M, Katsuya T, Sugiyama T, Yamaguchi S, et al. Evaluation of genetic loci influencing adult height in the Japanese population. J Hum Genet 2009; 54:749 - 52; http://dx.doi.org/10.1038/jhg.2009.99; PMID: 19834501
   PubMed Web of Science ®Google Scholar
• Widén E, Ripatti S, Cousminer DL, Surakka I, Lappalainen T, Järvelin MR, et al. Distinct variants at LIN28B influence growth in height from birth to adulthood. Am J Hum Genet 2010; 86:773 - 82; http://dx.doi.org/10.1016/j.ajhg.2010.03.010; PMID: 20398887
   PubMed Web of Science ®Google Scholar
• Monk D, Arnaud P, Apostolidou S, Hills FA, Kelsey G, Stanier P, et al. Limited evolutionary conservation of imprinting in the human placenta. Proc Natl Acad Sci U S A 2006; 103:6623 - 8; http://dx.doi.org/10.1073/pnas.0511031103; PMID: 16614068
   PubMed Web of Science ®Google Scholar
• Blahnik KR, Dou L, Echipare L, Iyengar S, O’Geen H, Sanchez E, et al. Characterization of the contradictory chromatin signatures at the 3′ exons of zinc finger genes. PLoS One 2011; 6:e17121; http://dx.doi.org/10.1371/journal.pone.0017121; PMID: 21347206
   PubMedGoogle Scholar
• Li J, Bench AJ, Vassiliou GS, Fourouclas N, Ferguson-Smith AC, Green AR. Imprinting of the human L3MBTL gene, a polycomb family member located in a region of chromosome 20 deleted in human myeloid malignancies. Proc Natl Acad Sci U S A 2004; 101:7341 - 6; http://dx.doi.org/10.1073/pnas.0308195101; PMID: 15123827
   PubMed Web of Science ®Google Scholar
• Iida J, Ishizaki H, Okamoto-Tanaka M, Kawata A, Sumita K, Ohgake S, et al. Synaptic scaffolding molecule alpha is a scaffold to mediate N-methyl-D-aspartate receptor-dependent RhoA activation in dendrites. Mol Cell Biol 2007; 27:4388 - 405; http://dx.doi.org/10.1128/MCB.01901-06; PMID: 17438139
   PubMed Web of Science ®Google Scholar
• Kim J, Bergmann A, Lucas S, Stone R, Stubbs L. Lineage-specific imprinting and evolution of the zinc-finger gene ZIM2. Genomics 2004; 84:47 - 58; http://dx.doi.org/10.1016/j.ygeno.2004.02.007; PMID: 15203203
   PubMed Web of Science ®Google Scholar
• Zhang Y, Guan DG, Yang JH, Shao P, Zhou H, Qu LH. ncRNAimprint: a comprehensive database of mammalian imprinted noncoding RNAs. RNA 2010; 16:1889 - 901; http://dx.doi.org/10.1261/rna.2226910; PMID: 20801769
   PubMed Web of Science ®Google Scholar
• Koyanagi M, Nakabayashi K, Fujimoto T, Gu N, Baba I, Takashima Y, et al. ZFAT expression in B and T lymphocytes and identification of ZFAT-regulated genes. Genomics 2008; 91:451 - 7; http://dx.doi.org/10.1016/j.ygeno.2008.01.009; PMID: 18329245
   PubMed Web of Science ®Google Scholar
• Coan PM, Burton GJ, Ferguson-Smith AC. Imprinted genes in the placenta--a review. Placenta 2005; 26:Suppl A S10 - 20; http://dx.doi.org/10.1016/j.placenta.2004.12.009; PMID: 15837057
   PubMedGoogle Scholar
• Pham NV, Nguyen MT, Hu JF, Vu TH, Hoffman AR. Dissociation of IGF2 and H19 imprinting in human brain. Brain Res 1998; 810:1 - 8; http://dx.doi.org/10.1016/S0006-8993(98)00783-5; PMID: 9813220
   PubMed Web of Science ®Google Scholar
• Tsunoda T, Takashima Y, Tanaka Y, Fujimoto T, Doi K, Hirose Y, et al. Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands. Proc Natl Acad Sci U S A 2010; 107:14199 - 204; http://dx.doi.org/10.1073/pnas.1002494107; PMID: 20660741
   PubMed Web of Science ®Google Scholar
• Winn VD, Haimov-Kochman R, Paquet AC, Yang YJ, Madhusudhan MS, Gormley M, et al. Gene expression profiling of the human maternal-fetal interface reveals dramatic changes between midgestation and term. Endocrinology 2007; 148:1059 - 79; http://dx.doi.org/10.1210/en.2006-0683; PMID: 17170095
   PubMed Web of Science ®Google Scholar
• Slavin TP, Feng T, Schnell A, Zhu X, Elston RC. Two-marker association tests yield new disease associations for coronary artery disease and hypertension. Hum Genet 2011; 130:725 - 33; http://dx.doi.org/10.1007/s00439-011-1009-6; PMID: 21626137
   PubMed Web of Science ®Google Scholar
• Okae H, Hiura H, Nishida Y, Funayama R, Tanaka S, Chiba H, et al. Re-investigation and RNA sequencing-based identification of genes with placenta-specific imprinted expression. Hum Mol Genet 2012; 21:548 - 58; http://dx.doi.org/10.1093/hmg/ddr488; PMID: 22025075
   PubMed Web of Science ®Google Scholar
• Proudhon C, Bourc’his D. Identification and resolution of artifacts in the interpretation of imprinted gene expression. Brief Funct Genomics 2010; 9:374 - 84; http://dx.doi.org/10.1093/bfgp/elq020; PMID: 20829207
   PubMed Web of Science ®Google Scholar
• Wang X, Sun Q, McGrath SD, Mardis ER, Soloway PD, Clark AG. Transcriptome-wide identification of novel imprinted genes in neonatal mouse brain. PLoS One 2008; 3:e3839; http://dx.doi.org/10.1371/journal.pone.0003839; PMID: 19052635
   PubMed Web of Science ®Google Scholar
• Gascoin-Lachambre G, Buffat C, Rebourcet R, Chelbi ST, Rigourd V, Mondon F, et al. Cullins in human intra-uterine growth restriction: expressional and epigenetic alterations. Placenta 2010; 31:151 - 7; http://dx.doi.org/10.1016/j.placenta.2009.11.008; PMID: 20005570
   PubMed Web of Science ®Google Scholar
• Mondon F, Mignot TM, Rebourcet R, Jammes H, Danan JL, Ferré F, et al. Profiling of oxygen-modulated gene expression in early human placenta by systematic sequencing of suppressive subtractive hybridization products. Physiol Genomics 2005; 22:99 - 107; http://dx.doi.org/10.1152/physiolgenomics.00276.2004; PMID: 15797968
   PubMed Web of Science ®Google Scholar
• Borghese B, Mondon F, Noël JC, Fayt I, Mignot TM, Vaiman D, et al. Gene expression profile for ectopic versus eutopic endometrium provides new insights into endometriosis oncogenic potential. Mol Endocrinol 2008; 22:2557 - 62; http://dx.doi.org/10.1210/me.2008-0322; PMID: 18818281
   PubMed Web of Science ®Google Scholar
• Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 1987; 162:156 - 9; http://dx.doi.org/10.1016/0003-2697(87)90021-2; PMID: 2440339
   PubMed Web of Science ®Google Scholar
• Meller M, Vadachkoria S, Luthy DA, Williams MA. Evaluation of housekeeping genes in placental comparative expression studies. Placenta 2005; 26:601 - 7; http://dx.doi.org/10.1016/j.placenta.2004.09.009; PMID: 16085039
   PubMed Web of Science ®Google Scholar
• Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 2001; 29:e45; http://dx.doi.org/10.1093/nar/29.9.e45; PMID: 11328886
   PubMed Web of Science ®Google Scholar